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The map label for this gene is gluQ

Identifier: 34498888

GI number: 34498888

Start: 3730152

End: 3731048

Strand: Reverse

Name: gluQ

Synonym: CV_3433

Alternate gene names: 34498888

Gene position: 3731048-3730152 (Counterclockwise)

Preceding gene: 34498890

Following gene: 34498887

Centisome position: 78.53

GC content: 70.46

Gene sequence:

>897_bases
ATGTCGAATGCACTCTACCGGGGCCGTTTCGCCCCCAGTCCCACCGGCCTGCTGCACGCGGGTTCGCTCAGCACCGCCAT
CGGCAGTTACCTGGAAGCGCGCAGCCGAGGCGGCGAGTGGCTGCTGCGGATGGAAGACCTGGACCCGCCGCGCGAGGTGC
CCGGCGCGGCCGACGACATCCTGCGCACGCTGGAGGCATTCGGCTTCGAGTGGGACGGCGAGGTGGTTTACCAGAGCCGC
CGGCATGGCTTGTACCGCGCCGCGCTGGAGCGGCTGATCGCCTCAGGACGCGCTTACGCCTGCTGCTGCACCCGCAAGGA
GATCGCTGCGACGGCGCGGCGGGGCCTGGACGGCTACGTCTATCCCGGCACCTGCCGCAACGGTTGTCCAGACGGCCGCG
AGGGGCGCGCCTGGCGGCTGCGGGTGGATGAGGGCGAGTGGACGGCGCATGACCGGCTGCAGGGCGAGCATCGGCAGGAT
CTGGCGCGCGACATCGGCGACTTCGTGCTGCTGCGCGCTGACGGCTTCTGGGCCTATCAGCTGGCGGTGGTGGTCGACGA
CGCCGAGCAGGGCGTCACCGATATCGTCCGCGGCGCCGATCTGCTGGTGTCCACGCCGCGGCAACTGGCGGTTTACGACG
CGTTGGGCCAATCCGCGCCCGGCTATTGCCATCTGCCGGTGCTGACCAATGCCGCCGGCGAGAAGCTGTCCAAGCAGACG
CTGGCGCCGGCGATCTCGACCCGCGACGCCGCGCGGCAGCTGCGCGAGGCCCTGGCGTGGCTGGGCCATGTTCCGCCAGC
GGATTGCGGAAGCCTGGACGAGCTGTGGCCTTGGGCTGTGGCGAATTGGTCATTGAGCCGCGTGCCGGCCGGCCCGCTGC
AACTCCCTCTTTCCTGA

Upstream 100 bases:

>100_bases
TACGCCATTCTCGTATCCTCAGTAAGGCTGGGTCGTGATTCGGGATAATCGTCATTTTAGCGATAAATCTTCGTTCCGAC
GCTCCGGCAAATCCCTCACG

Downstream 100 bases:

>100_bases
CGACTTTTCATTGCGATGACATAAGCATCTGTCTGTTAACGGATTTTTGTTGAAAAAATCGTCAATGCGACATGTTTTTA
CGTCAAAACTGTGTTTTAAT

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSNALYRGRFAPSPTGLLHAGSLSTAIGSYLEARSRGGEWLLRMEDLDPPREVPGAADDILRTLEAFGFEWDGEVVYQSR
RHGLYRAALERLIASGRAYACCCTRKEIAATARRGLDGYVYPGTCRNGCPDGREGRAWRLRVDEGEWTAHDRLQGEHRQD
LARDIGDFVLLRADGFWAYQLAVVVDDAEQGVTDIVRGADLLVSTPRQLAVYDALGQSAPGYCHLPVLTNAAGEKLSKQT
LAPAISTRDAARQLREALAWLGHVPPADCGSLDELWPWAVANWSLSRVPAGPLQLPLS

Sequences:

>Translated_298_residues
MSNALYRGRFAPSPTGLLHAGSLSTAIGSYLEARSRGGEWLLRMEDLDPPREVPGAADDILRTLEAFGFEWDGEVVYQSR
RHGLYRAALERLIASGRAYACCCTRKEIAATARRGLDGYVYPGTCRNGCPDGREGRAWRLRVDEGEWTAHDRLQGEHRQD
LARDIGDFVLLRADGFWAYQLAVVVDDAEQGVTDIVRGADLLVSTPRQLAVYDALGQSAPGYCHLPVLTNAAGEKLSKQT
LAPAISTRDAARQLREALAWLGHVPPADCGSLDELWPWAVANWSLSRVPAGPLQLPLS
>Mature_297_residues
SNALYRGRFAPSPTGLLHAGSLSTAIGSYLEARSRGGEWLLRMEDLDPPREVPGAADDILRTLEAFGFEWDGEVVYQSRR
HGLYRAALERLIASGRAYACCCTRKEIAATARRGLDGYVYPGTCRNGCPDGREGRAWRLRVDEGEWTAHDRLQGEHRQDL
ARDIGDFVLLRADGFWAYQLAVVVDDAEQGVTDIVRGADLLVSTPRQLAVYDALGQSAPGYCHLPVLTNAAGEKLSKQTL
APAISTRDAARQLREALAWLGHVPPADCGSLDELWPWAVANWSLSRVPAGPLQLPLS

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=251, Percent_Identity=37.8486055776892, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI226510925, Length=293, Percent_Identity=48.122866894198, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1788743, Length=245, Percent_Identity=33.0612244897959, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=35.4581673306773, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6324540, Length=299, Percent_Identity=34.1137123745819, Blast_Score=152, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24665321, Length=251, Percent_Identity=35.8565737051793, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_CHRVO (Q7NSJ1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903103.1
- ProteinModelPortal:   Q7NSJ1
- SMR:   Q7NSJ1
- GeneID:   2548309
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3433
- NMPDR:   fig|243365.1.peg.3433
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- PhylomeDB:   Q7NSJ1
- ProtClustDB:   PRK05710
- BioCyc:   CVIO243365:CV_3433-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: 6.1.1.17

Molecular weight: Translated: 32637; Mature: 32506

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 45-45 BINDING 179-179 BINDING 197-197 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNALYRGRFAPSPTGLLHAGSLSTAIGSYLEARSRGGEWLLRMEDLDPPREVPGAADDI
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCHHHH
LRTLEAFGFEWDGEVVYQSRRHGLYRAALERLIASGRAYACCCTRKEIAATARRGLDGYV
HHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEE
YPGTCRNGCPDGREGRAWRLRVDEGEWTAHDRLQGEHRQDLARDIGDFVLLRADGFWAYQ
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHEEEEEECCCEEEE
LAVVVDDAEQGVTDIVRGADLLVSTPRQLAVYDALGQSAPGYCHLPVLTNAAGEKLSKQT
EEEEEECHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHH
LAPAISTRDAARQLREALAWLGHVPPADCGSLDELWPWAVANWSLSRVPAGPLQLPLS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNALYRGRFAPSPTGLLHAGSLSTAIGSYLEARSRGGEWLLRMEDLDPPREVPGAADDI
CCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCHHHH
LRTLEAFGFEWDGEVVYQSRRHGLYRAALERLIASGRAYACCCTRKEIAATARRGLDGYV
HHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEE
YPGTCRNGCPDGREGRAWRLRVDEGEWTAHDRLQGEHRQDLARDIGDFVLLRADGFWAYQ
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHEEEEEECCCEEEE
LAVVVDDAEQGVTDIVRGADLLVSTPRQLAVYDALGQSAPGYCHLPVLTNAAGEKLSKQT
EEEEEECHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHH
LAPAISTRDAARQLREALAWLGHVPPADCGSLDELWPWAVANWSLSRVPAGPLQLPLS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782