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The map label for this gene is bigR [H]

Identifier: 34498806

GI number: 34498806

Start: 3646071

End: 3646364

Strand: Reverse

Name: bigR [H]

Synonym: CV_3351

Alternate gene names: 34498806

Gene position: 3646364-3646071 (Counterclockwise)

Preceding gene: 34498816

Following gene: 34498804

Centisome position: 76.75

GC content: 58.16

Gene sequence:

>294_bases
CTGTTCAACGACGACCAGATCGAGCAGACGTCGCGGGCGATGAAGGCCATGTCGCATCCGCTGCGTTTGAAGATCATATC
GGTGCTGGAAGACAGGGAGGTGAGCGTGCAAGACATCGTCGAGCAGGTCGGCACTTCCAAGAGCAATATTTCCCAGCACT
TGGCCATTATGCGGGACAAGGGCGTTTTGCGCACGCGCAAGGACGCCAACCGGGTTTACTACCGGGTGGGCGACATCCGC
ACGCTGGACGTGCTGAGGATGATGCGCGAAGTGTTCTGTGGTTTCGGGGAGTAA

Upstream 100 bases:

>100_bases
TCAGAATATTAGAAAACCTTGATAGTATCTGGTTTTGTGGATACTATTGTACCGCAGTCAAATCAAAAAAATGTCAGGCT
ACATTAGGAGAAAAGCTTTG

Downstream 100 bases:

>100_bases
GCGCTTCACGGGACCCATATGGAAAAAGCCGCGAAACGCGGCTTTTTCCATATGGGTTTCGCGCTTGCAGCCGCTAACAA
AACCTTGTAGAGCGTCTGAG

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 97

Protein sequence:

>97_residues
MFNDDQIEQTSRAMKAMSHPLRLKIISVLEDREVSVQDIVEQVGTSKSNISQHLAIMRDKGVLRTRKDANRVYYRVGDIR
TLDVLRMMREVFCGFGE

Sequences:

>Translated_97_residues
MFNDDQIEQTSRAMKAMSHPLRLKIISVLEDREVSVQDIVEQVGTSKSNISQHLAIMRDKGVLRTRKDANRVYYRVGDIR
TLDVLRMMREVFCGFGE
>Mature_97_residues
MFNDDQIEQTSRAMKAMSHPLRLKIISVLEDREVSVQDIVEQVGTSKSNISQHLAIMRDKGVLRTRKDANRVYYRVGDIR
TLDVLRMMREVFCGFGE

Specific function: Represses an operon that comprises itself, XF_0764, XF_0765, XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=87, Percent_Identity=35.632183908046, Blast_Score=63, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 11244; Mature: 11244

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNDDQIEQTSRAMKAMSHPLRLKIISVLEDREVSVQDIVEQVGTSKSNISQHLAIMRDK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCC
GVLRTRKDANRVYYRVGDIRTLDVLRMMREVFCGFGE
CCHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNDDQIEQTSRAMKAMSHPLRLKIISVLEDREVSVQDIVEQVGTSKSNISQHLAIMRDK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCC
GVLRTRKDANRVYYRVGDIRTLDVLRMMREVFCGFGE
CCHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]