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The map label for this gene is kefB [H]

Identifier: 34498781

GI number: 34498781

Start: 3624631

End: 3626601

Strand: Reverse

Name: kefB [H]

Synonym: CV_3326

Alternate gene names: 34498781

Gene position: 3626601-3624631 (Counterclockwise)

Preceding gene: 34498797

Following gene: 34498774

Centisome position: 76.33

GC content: 66.87

Gene sequence:

>1971_bases
ATGCATTCCATGGCCCCCATCGTCCTCGTCCTGCTGGCCGCCGTGCTGGTGGTCACCCTGTGCCGCAGCCTGAAGGTGCC
GGCCATGCTCGGCTACCTGGTGGTGGGCTTCCTGACGGGGCCGGGGGTGATGAACCTGATTCCGCAGGGCAAGGAGACTG
CCTTCCTCGGCGAGATCGGCATCGTGTTCATGATGTTCACCATAGGCCTGGAGTTTTCGCTGCCCAGGCTGAGGGCGATG
CGCCAGCTGGTGTTCGGCGTCGGTTTCGCCCAGGTGGCGCTGACCATGCTGCTGGTGGCGATGGCCATCGCCTATCTGAC
AGGCAGCCCGCTGACTGGTTTCGCCATCGGCGGGGCATTGGCGATGTCGTCCACCGCCATCGTCAGCAAACTGCTGGCGG
AACGGCTGGAATTGAACCAGCCGCACGGCCAGCTGGCGATCGGCGTGCTGCTGTTCCAGGACATCGCCGTGGTGCCGCTG
CTGATCATGCTGCCGGCCTTCGCCGGCGGCAGCGACACGCTGTGGCTGGATCTGGCCAAGGCGGGCGGCAAGGTGCTGGT
GGTGCTGGCCTTGCTGCTGTTCTTCGGCCAGCGTCTGGTGCGGCCCTGGTTCCACCTGGTGGCGCGCCAGCGTTCCGGCG
AATTGTTCATGATCAACGTGCTGCTGGTGACGCTGGGCATCGCCTGGATGACCGAGCTCTCCGGCCTCAGCCTGGCGCTG
GGGGCCTTCGTCGCCGGCATGCTGATCTCGGAAACCGAGTACCGCTACCAGGTGGAGGAGGACATCAAGCCTTTCCGCGA
CATCCTGCTGGGTTTCTTCTTCATCACCGTGGGCATGAAGCTGGAATTGTCGGTGCTGTTCGGCCGCTTCGGCGAGGTTT
TGCTGTTGCTGGCGCTGCTGCTGCCTTTGAAGCTGGCGGTGGTGTTCGGTCTGGGCCGTTTGTTCGGCCATCGTTCCAAC
GACGCGATGCGCTCGGCGCTGGCTCTGGCCCAGGGCGGCGAGTTCGGCTTCGTGCTGCTGTCGCTGGCGCTGAACTTGAA
GCTGGTGGCCGCGCCGATGGCCCAGGCCGCCATCGCCGCGATACTGATCTCCATGCTGGCGGCGCCTTTCCTGATCATGC
ACGGCGAGCGCATCACCCGCAGGCTGATCAAGCAGGACTGGATGCTGCAATCGCTGGACCTGCACCAGATGCTGGTGGCC
GGCATGAGCAAGGACGAGCATGTGCTGATTTGCGGCTACGGCCGCAGCGGCCAGGCGCTGGCCCGTTTGCTGGAGACCGA
GAAGATCAATATGTTCGCGCTGGACATGGATCCGGAGCGGGTGAGGGAGGCGGGCGAGGCCGGCGACCCGGTGGTGTTCG
GCGACGCCGGCAAGAAGGAGGTGCTGCTCGCCGCCGGCCTGATGCGCGCCAAGGTGGTGGTGGTGACCTTCGCCGACACC
CATGCCGCCTTGCGCATTTTGCACACGGTGCGCGAGGTGCGGCCGGATCTGCCGGTGATCGTGCGCACCATAGATGAAAG
CGAGCTGGACGTGCTGCGCCAGGCCGGCGCCGACGAGGTGGTGGCCGAGGTGATGGAAGGCAGCCTGATGCTGGCCTCGC
AGGCGCTGCTGGAGGCGGGCGTGCCGCCCAGCCGGGTGCTGCGGCGCATCCGCGCGGTGCGCGAGGAACGCTACGATCTG
TTCCGCGGCTTCTTCCGCGGCAGCAGCGACGAGACGGAAAGCCTGGAGGAGTCGCTGGTGCCCCGGCTGCTGAGCGTGCA
GGTATGCGGCGGCGCGGCGGCGATCGGCCAGCCGCTGGGCGCGCTGGGCCTGGACGCGCTGGGCGTGGAGCTGAAGGCGA
TCCGCCGCAACCGCGTGCGCCGCACCGATTTCGACGACAGCTTCGAGGTGGCGCCGGACGACGTGATGGTGCTGCTCGGC
ACGCCGGAGCAGCTGGCGCTGGCTGAGTCGAGAATCCTGCAGGGAGATTGA

Upstream 100 bases:

>100_bases
ATATCAAAGGTTATAATCGATTGACGCGGGTGCTGGTGCAAGTTTTGCTTGGCGGTCGTGTTTTCCGTTGCCAATTCTTA
CCCAGGAGCCCCGTCCGCCG

Downstream 100 bases:

>100_bases
GCCCCGCGCCCAATGAAAAAGCCCCGGTTTCCCGGGGCTTTTCGTTTGGGGCCGTCGGCTCAGGGCAGTATCGTGGTGAT
CTGCTTCTGAAGCTGCTGCT

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MHSMAPIVLVLLAAVLVVTLCRSLKVPAMLGYLVVGFLTGPGVMNLIPQGKETAFLGEIGIVFMMFTIGLEFSLPRLRAM
RQLVFGVGFAQVALTMLLVAMAIAYLTGSPLTGFAIGGALAMSSTAIVSKLLAERLELNQPHGQLAIGVLLFQDIAVVPL
LIMLPAFAGGSDTLWLDLAKAGGKVLVVLALLLFFGQRLVRPWFHLVARQRSGELFMINVLLVTLGIAWMTELSGLSLAL
GAFVAGMLISETEYRYQVEEDIKPFRDILLGFFFITVGMKLELSVLFGRFGEVLLLLALLLPLKLAVVFGLGRLFGHRSN
DAMRSALALAQGGEFGFVLLSLALNLKLVAAPMAQAAIAAILISMLAAPFLIMHGERITRRLIKQDWMLQSLDLHQMLVA
GMSKDEHVLICGYGRSGQALARLLETEKINMFALDMDPERVREAGEAGDPVVFGDAGKKEVLLAAGLMRAKVVVVTFADT
HAALRILHTVREVRPDLPVIVRTIDESELDVLRQAGADEVVAEVMEGSLMLASQALLEAGVPPSRVLRRIRAVREERYDL
FRGFFRGSSDETESLEESLVPRLLSVQVCGGAAAIGQPLGALGLDALGVELKAIRRNRVRRTDFDDSFEVAPDDVMVLLG
TPEQLALAESRILQGD

Sequences:

>Translated_656_residues
MHSMAPIVLVLLAAVLVVTLCRSLKVPAMLGYLVVGFLTGPGVMNLIPQGKETAFLGEIGIVFMMFTIGLEFSLPRLRAM
RQLVFGVGFAQVALTMLLVAMAIAYLTGSPLTGFAIGGALAMSSTAIVSKLLAERLELNQPHGQLAIGVLLFQDIAVVPL
LIMLPAFAGGSDTLWLDLAKAGGKVLVVLALLLFFGQRLVRPWFHLVARQRSGELFMINVLLVTLGIAWMTELSGLSLAL
GAFVAGMLISETEYRYQVEEDIKPFRDILLGFFFITVGMKLELSVLFGRFGEVLLLLALLLPLKLAVVFGLGRLFGHRSN
DAMRSALALAQGGEFGFVLLSLALNLKLVAAPMAQAAIAAILISMLAAPFLIMHGERITRRLIKQDWMLQSLDLHQMLVA
GMSKDEHVLICGYGRSGQALARLLETEKINMFALDMDPERVREAGEAGDPVVFGDAGKKEVLLAAGLMRAKVVVVTFADT
HAALRILHTVREVRPDLPVIVRTIDESELDVLRQAGADEVVAEVMEGSLMLASQALLEAGVPPSRVLRRIRAVREERYDL
FRGFFRGSSDETESLEESLVPRLLSVQVCGGAAAIGQPLGALGLDALGVELKAIRRNRVRRTDFDDSFEVAPDDVMVLLG
TPEQLALAESRILQGD
>Mature_656_residues
MHSMAPIVLVLLAAVLVVTLCRSLKVPAMLGYLVVGFLTGPGVMNLIPQGKETAFLGEIGIVFMMFTIGLEFSLPRLRAM
RQLVFGVGFAQVALTMLLVAMAIAYLTGSPLTGFAIGGALAMSSTAIVSKLLAERLELNQPHGQLAIGVLLFQDIAVVPL
LIMLPAFAGGSDTLWLDLAKAGGKVLVVLALLLFFGQRLVRPWFHLVARQRSGELFMINVLLVTLGIAWMTELSGLSLAL
GAFVAGMLISETEYRYQVEEDIKPFRDILLGFFFITVGMKLELSVLFGRFGEVLLLLALLLPLKLAVVFGLGRLFGHRSN
DAMRSALALAQGGEFGFVLLSLALNLKLVAAPMAQAAIAAILISMLAAPFLIMHGERITRRLIKQDWMLQSLDLHQMLVA
GMSKDEHVLICGYGRSGQALARLLETEKINMFALDMDPERVREAGEAGDPVVFGDAGKKEVLLAAGLMRAKVVVVTFADT
HAALRILHTVREVRPDLPVIVRTIDESELDVLRQAGADEVVAEVMEGSLMLASQALLEAGVPPSRVLRRIRAVREERYDL
FRGFFRGSSDETESLEESLVPRLLSVQVCGGAAAIGQPLGALGLDALGVELKAIRRNRVRRTDFDDSFEVAPDDVMVLLG
TPEQLALAESRILQGD

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=384, Percent_Identity=28.3854166666667, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789749, Length=545, Percent_Identity=34.4954128440367, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1786232, Length=564, Percent_Identity=32.4468085106383, Blast_Score=258, Evalue=9e-70,
Organism=Escherichia coli, GI1786685, Length=536, Percent_Identity=32.089552238806, Blast_Score=227, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71053; Mature: 71053

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSMAPIVLVLLAAVLVVTLCRSLKVPAMLGYLVVGFLTGPGVMNLIPQGKETAFLGEIG
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHH
IVFMMFTIGLEFSLPRLRAMRQLVFGVGFAQVALTMLLVAMAIAYLTGSPLTGFAIGGAL
HHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AMSSTAIVSKLLAERLELNQPHGQLAIGVLLFQDIAVVPLLIMLPAFAGGSDTLWLDLAK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHH
AGGKVLVVLALLLFFGQRLVRPWFHLVARQRSGELFMINVLLVTLGIAWMTELSGLSLAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHH
GAFVAGMLISETEYRYQVEEDIKPFRDILLGFFFITVGMKLELSVLFGRFGEVLLLLALL
HHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LPLKLAVVFGLGRLFGHRSNDAMRSALALAQGGEFGFVLLSLALNLKLVAAPMAQAAIAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILISMLAAPFLIMHGERITRRLIKQDWMLQSLDLHQMLVAGMSKDEHVLICGYGRSGQAL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCHHHH
ARLLETEKINMFALDMDPERVREAGEAGDPVVFGDAGKKEVLLAAGLMRAKVVVVTFADT
HHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCEEEEEEECCH
HAALRILHTVREVRPDLPVIVRTIDESELDVLRQAGADEVVAEVMEGSLMLASQALLEAG
HHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCC
VPPSRVLRRIRAVREERYDLFRGFFRGSSDETESLEESLVPRLLSVQVCGGAAAIGQPLG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHH
ALGLDALGVELKAIRRNRVRRTDFDDSFEVAPDDVMVLLGTPEQLALAESRILQGD
HHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHSMAPIVLVLLAAVLVVTLCRSLKVPAMLGYLVVGFLTGPGVMNLIPQGKETAFLGEIG
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHH
IVFMMFTIGLEFSLPRLRAMRQLVFGVGFAQVALTMLLVAMAIAYLTGSPLTGFAIGGAL
HHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AMSSTAIVSKLLAERLELNQPHGQLAIGVLLFQDIAVVPLLIMLPAFAGGSDTLWLDLAK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHH
AGGKVLVVLALLLFFGQRLVRPWFHLVARQRSGELFMINVLLVTLGIAWMTELSGLSLAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHH
GAFVAGMLISETEYRYQVEEDIKPFRDILLGFFFITVGMKLELSVLFGRFGEVLLLLALL
HHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LPLKLAVVFGLGRLFGHRSNDAMRSALALAQGGEFGFVLLSLALNLKLVAAPMAQAAIAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILISMLAAPFLIMHGERITRRLIKQDWMLQSLDLHQMLVAGMSKDEHVLICGYGRSGQAL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCHHHH
ARLLETEKINMFALDMDPERVREAGEAGDPVVFGDAGKKEVLLAAGLMRAKVVVVTFADT
HHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCEEEEEEECCH
HAALRILHTVREVRPDLPVIVRTIDESELDVLRQAGADEVVAEVMEGSLMLASQALLEAG
HHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCC
VPPSRVLRRIRAVREERYDLFRGFFRGSSDETESLEESLVPRLLSVQVCGGAAAIGQPLG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHH
ALGLDALGVELKAIRRNRVRRTDFDDSFEVAPDDVMVLLGTPEQLALAESRILQGD
HHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA