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The map label for this gene is pleD [H]

Identifier: 34498707

GI number: 34498707

Start: 3535824

End: 3537545

Strand: Direct

Name: pleD [H]

Synonym: CV_3252

Alternate gene names: 34498707

Gene position: 3535824-3537545 (Clockwise)

Preceding gene: 34498706

Following gene: 34498709

Centisome position: 74.42

GC content: 69.8

Gene sequence:

>1722_bases
ATGCGCCTGTCCAAGATATTCACCGTCGTCACCCTTGTCCTGTTCGTGCTCGCCTGCCTGCCGCTGGCCTGGATAGTCAA
TGGCGAGTGGGCGGTGTACCGCGCCGCCGACGAGGGGCTGGCTTCGATACAGATCGCCCATCTCGCCATGGTGGCGGTAG
AGAAGATTTCCTTCGAGCGGGGCCCGGCCAATGTCCTGATCGGCTCGCCGGCCGATCAGGACGCCCGCGAGCGGCTGCGC
CGCGCCCGGCAGACCAGCGACCGCGCGCTGCAGGATCTGGTCTCGGCCCTGTCGCCCTCGAGCTCCGCCTACCACCGGCA
GGCGCTGGCCGCGGTGCGCTCCGCCCGGCAGCGCTTGAGCGAGGCGCGCCTGGGCATGGACCGGGTCATCGCCTCGCCGA
GGCCCGCTTCGGCCGGGATCATGCCGTCTATCCGGCGGATGTTCGACGTGGTGCCCGCCATTCTCGAGGCGGTGACCGCC
CTCTCCTCGCAGGCATCGAGCGTTTATCCCCAGTTGTCCCATCCGCTGAGCGGCGCCCGCATGGCGACCGAACTGCGCGA
ATACGCCGGCCGGCTGGGCTCGACGCTGACGCCCGCGCTGGCCGAGCAACGGCCGCTGGCCGAAAGCGAAACCCGGGACA
TCCACATCCTGCGCGGTCGCATCGAACAGCTGCGCGGCACGATACTGCAGCGCCTCAACGCTCCGTCCCTGCAACCGGGA
CCGCGCCAGGCGGTGGGAGACATGGAAAGCCTCTATTTCCGCGAAGGACTGGCGCAGGTGGACGACATCCTGCTCGACAG
CCACCTCGGCCGCCCTTACCGGGTCAGCCTGGCCGAATTCACCGCCGGCTACGTGCCTACCCTGTACAGCATCGTCGAAC
TGCGCGACGCCATGCTGACCGCGGCGGCGGAGGAGGCCCGCGTCCAGCGGAGCGCCGCCTTCCGCCACCTGCTGGGCACC
ATCGCGCTGGGCGTGGTCGCACTGATCGCGCAACTGGCGCTGTTCGCCTACATCCGCTGGCTGATCGCCAGGCCGCTGCT
GGCCGCGTCGCGGCTGCTGTCCGACCTCGCCGACGGCAAGCTGGACGCGGATATTCCCGACAGCCACCGGAAAGACGAAA
TCGGCGACGTGCTGCGCGCCATCGCCATCCTGCGGCGCAACAGCCAGGAAAAGCGCGCGCTGGAGCATGAGCGCCAAAGG
TTGATCGACGAATTGACCGAGATGTCGCGGATCGACTTCCTGACCGGCATCACCAACCGGCGAGCGTTCACGCAGGCCGC
CCACGCCGAGATCGCGCGCGCCGGCCTGGCGCGGGAGTCGGCGACGCTGATCCTGTTCGACATCGATCACTTCAAGCAGG
TGAACGACCTGTACGGGCACGACTGCGGCGACGCGGTGCTGGTCCACGTCGCGGCCATCGTCGAGCGCGTCTGCCGCGAG
GGCGACCTGGCCGGCCGCTACGGCGGCGAGGAGTTCGTGGTGCTGGCCTGCTACCGCGGGGCGTCGAACGGCGAGGCGCT
GGCCGAACGGCTGCGCGCTGCCATCGCGGAATCGCCGTGCTTGCTGCCGTCCGGCCAGAAGCTGGCGGTCACCGCCAGTT
TCGGCGTCGCCACGCTGGGCGAAGCCGCTCCCTCGCTTGAGCGGCTGCTGCAGGCCGCCGACCGCGCGCTGTACGCGGCG
AAGCGGCTGGGGCGGAACCGGGTGATGGTGGACGCCAGCTGA

Upstream 100 bases:

>100_bases
CCGCAGCGGCAAATTTTCCTGCCGCGCCGCCGCCGCGCATATACTCAATCTTGCACAGCGCCTGCGCTGAACGCAGCATT
CCTTGCACTGAACGAGATCG

Downstream 100 bases:

>100_bases
CCTCGCCCCCGGCGCCCGTCACGCCTGCAGCCTGCCGAGTTCCCTCAGGCGCTCTCCGATGCGGGCGATTTCCGCTTCGC
CGGCCGCCAGCGCTTCCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRLSKIFTVVTLVLFVLACLPLAWIVNGEWAVYRAADEGLASIQIAHLAMVAVEKISFERGPANVLIGSPADQDARERLR
RARQTSDRALQDLVSALSPSSSAYHRQALAAVRSARQRLSEARLGMDRVIASPRPASAGIMPSIRRMFDVVPAILEAVTA
LSSQASSVYPQLSHPLSGARMATELREYAGRLGSTLTPALAEQRPLAESETRDIHILRGRIEQLRGTILQRLNAPSLQPG
PRQAVGDMESLYFREGLAQVDDILLDSHLGRPYRVSLAEFTAGYVPTLYSIVELRDAMLTAAAEEARVQRSAAFRHLLGT
IALGVVALIAQLALFAYIRWLIARPLLAASRLLSDLADGKLDADIPDSHRKDEIGDVLRAIAILRRNSQEKRALEHERQR
LIDELTEMSRIDFLTGITNRRAFTQAAHAEIARAGLARESATLILFDIDHFKQVNDLYGHDCGDAVLVHVAAIVERVCRE
GDLAGRYGGEEFVVLACYRGASNGEALAERLRAAIAESPCLLPSGQKLAVTASFGVATLGEAAPSLERLLQAADRALYAA
KRLGRNRVMVDAS

Sequences:

>Translated_573_residues
MRLSKIFTVVTLVLFVLACLPLAWIVNGEWAVYRAADEGLASIQIAHLAMVAVEKISFERGPANVLIGSPADQDARERLR
RARQTSDRALQDLVSALSPSSSAYHRQALAAVRSARQRLSEARLGMDRVIASPRPASAGIMPSIRRMFDVVPAILEAVTA
LSSQASSVYPQLSHPLSGARMATELREYAGRLGSTLTPALAEQRPLAESETRDIHILRGRIEQLRGTILQRLNAPSLQPG
PRQAVGDMESLYFREGLAQVDDILLDSHLGRPYRVSLAEFTAGYVPTLYSIVELRDAMLTAAAEEARVQRSAAFRHLLGT
IALGVVALIAQLALFAYIRWLIARPLLAASRLLSDLADGKLDADIPDSHRKDEIGDVLRAIAILRRNSQEKRALEHERQR
LIDELTEMSRIDFLTGITNRRAFTQAAHAEIARAGLARESATLILFDIDHFKQVNDLYGHDCGDAVLVHVAAIVERVCRE
GDLAGRYGGEEFVVLACYRGASNGEALAERLRAAIAESPCLLPSGQKLAVTASFGVATLGEAAPSLERLLQAADRALYAA
KRLGRNRVMVDAS
>Mature_573_residues
MRLSKIFTVVTLVLFVLACLPLAWIVNGEWAVYRAADEGLASIQIAHLAMVAVEKISFERGPANVLIGSPADQDARERLR
RARQTSDRALQDLVSALSPSSSAYHRQALAAVRSARQRLSEARLGMDRVIASPRPASAGIMPSIRRMFDVVPAILEAVTA
LSSQASSVYPQLSHPLSGARMATELREYAGRLGSTLTPALAEQRPLAESETRDIHILRGRIEQLRGTILQRLNAPSLQPG
PRQAVGDMESLYFREGLAQVDDILLDSHLGRPYRVSLAEFTAGYVPTLYSIVELRDAMLTAAAEEARVQRSAAFRHLLGT
IALGVVALIAQLALFAYIRWLIARPLLAASRLLSDLADGKLDADIPDSHRKDEIGDVLRAIAILRRNSQEKRALEHERQR
LIDELTEMSRIDFLTGITNRRAFTQAAHAEIARAGLARESATLILFDIDHFKQVNDLYGHDCGDAVLVHVAAIVERVCRE
GDLAGRYGGEEFVVLACYRGASNGEALAERLRAAIAESPCLLPSGQKLAVTASFGVATLGEAAPSLERLLQAADRALYAA
KRLGRNRVMVDAS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=37.6470588235294, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=40, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=35.9550561797753, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=39.7515527950311, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=39.0243902439024, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.3373493975904, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788956, Length=213, Percent_Identity=32.3943661971831, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=27.7456647398844, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=32.7044025157233, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=29.4478527607362, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=185, Percent_Identity=30.2702702702703, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62502; Mature: 62502

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSKIFTVVTLVLFVLACLPLAWIVNGEWAVYRAADEGLASIQIAHLAMVAVEKISFER
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
GPANVLIGSPADQDARERLRRARQTSDRALQDLVSALSPSSSAYHRQALAAVRSARQRLS
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EARLGMDRVIASPRPASAGIMPSIRRMFDVVPAILEAVTALSSQASSVYPQLSHPLSGAR
HHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHH
MATELREYAGRLGSTLTPALAEQRPLAESETRDIHILRGRIEQLRGTILQRLNAPSLQPG
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PRQAVGDMESLYFREGLAQVDDILLDSHLGRPYRVSLAEFTAGYVPTLYSIVELRDAMLT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHH
AAAEEARVQRSAAFRHLLGTIALGVVALIAQLALFAYIRWLIARPLLAASRLLSDLADGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDADIPDSHRKDEIGDVLRAIAILRRNSQEKRALEHERQRLIDELTEMSRIDFLTGITNR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RAFTQAAHAEIARAGLARESATLILFDIDHFKQVNDLYGHDCGDAVLVHVAAIVERVCRE
HHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GDLAGRYGGEEFVVLACYRGASNGEALAERLRAAIAESPCLLPSGQKLAVTASFGVATLG
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHH
EAAPSLERLLQAADRALYAAKRLGRNRVMVDAS
HCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MRLSKIFTVVTLVLFVLACLPLAWIVNGEWAVYRAADEGLASIQIAHLAMVAVEKISFER
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
GPANVLIGSPADQDARERLRRARQTSDRALQDLVSALSPSSSAYHRQALAAVRSARQRLS
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EARLGMDRVIASPRPASAGIMPSIRRMFDVVPAILEAVTALSSQASSVYPQLSHPLSGAR
HHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHH
MATELREYAGRLGSTLTPALAEQRPLAESETRDIHILRGRIEQLRGTILQRLNAPSLQPG
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PRQAVGDMESLYFREGLAQVDDILLDSHLGRPYRVSLAEFTAGYVPTLYSIVELRDAMLT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHH
AAAEEARVQRSAAFRHLLGTIALGVVALIAQLALFAYIRWLIARPLLAASRLLSDLADGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDADIPDSHRKDEIGDVLRAIAILRRNSQEKRALEHERQRLIDELTEMSRIDFLTGITNR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RAFTQAAHAEIARAGLARESATLILFDIDHFKQVNDLYGHDCGDAVLVHVAAIVERVCRE
HHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GDLAGRYGGEEFVVLACYRGASNGEALAERLRAAIAESPCLLPSGQKLAVTASFGVATLG
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHH
EAAPSLERLLQAADRALYAAKRLGRNRVMVDAS
HCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA