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The map label for this gene is rpfG [H]

Identifier: 34498701

GI number: 34498701

Start: 3526400

End: 3527500

Strand: Reverse

Name: rpfG [H]

Synonym: CV_3246

Alternate gene names: 34498701

Gene position: 3527500-3526400 (Counterclockwise)

Preceding gene: 34498704

Following gene: 34498699

Centisome position: 74.25

GC content: 69.12

Gene sequence:

>1101_bases
ATGGAGAGGGCGACCGTGCTGATCGTCGACGACGCGCCGGAATGCCTGGCGGCGCTGGGCGAGCTGTTGCAGCCGCATTA
CCGGGTGCTGGTCGCCAATGGCGGACCGGCCGCGCTCAGGCTGGCCGAGGGTCCTATCCGGCCCGACCTGATCCTGCTGG
ACGTGATGATGCCGGAGATGGACGGCAGGGAGGTGTTCGCCGGGCTGCGCAAGCGCGAGGCCACGGCTGCGATTCCCGTC
GTTTTCGTCACCGCGATGAATGAATGGAGAGACGAACTGCTGGGGCTGGAATCCGGCGCCGTCGATTACATCACCAAGCC
CATCATTCCGCCGCTGGTGCTGGCGCGCGTGCGCACCCAGCTGGAGCTGAAACAGGCGCGCGACCGCCTGAGCGACCAGA
ACCGCTGGCTGGAGGTCGAGGTGTCCCGCCGCATGGCCGAGAACGATCTGACCCAGCGGATGGCCATCCACGCGCTGGCG
CGGCTGGCCGAGATGCGGGACCTGGAAACCGGCAACCATCTGTTGCGCACCCAGGGCTACGTCGATGCGCTGGCGAGGCG
CTTGCGGGAGTTGCCGCGCTACCGCGGCGAGCTGAGCGAGCGGCGGATCGAACTGATCGCCCGATCCGCGCCGCTGCACG
ATATCGGCAAGGTCGGAGTGCCGGACCGCATCCTGCTCAAGCCGGGCAGGCTGGACGCGGATGAATGGGACATCATGCGC
AGCCACGCCAGGCTGGGCGAGCAGGCGATCGCCCAGGCCGAGCGCGACATCGGCCACCAGGTGGAGTTTCTTTCCTGCGC
CAAGCAGATCGCCGGCCACCACCACGAGCGCTGGGATGGCGGCGGCTACCCGGACGGGCTGGCCGGCGAGCGGATTCCGC
TGCCGGCCAGGCTGATGGCGCTGGCCGACGTGTTCGACGCGCTGATCTCGCGCCGCTGCTACAAGGCGGCGATGTCGTTC
GAAGAAGCGAGGGGGCTGATCGCCGCCGGGCGCGGCAGCCAGTTCCAGCCGGAGCTGGTGGACTGTTTTCTCGACGGCTT
CGACGACTTCGCCGCCATCGCCGCCCGCCACGCCGACGAGCCGGAAGCGGTGGCGGGCTAG

Upstream 100 bases:

>100_bases
GGCAGCCCGGCTTCATGCCGGATGGACAATTCGACGTTTGCCTAGGAGCGGGCGGCCGCGTACGGTGAATAGGAGGGCGG
CGAGACAAGGGGAGGGGATG

Downstream 100 bases:

>100_bases
CGCGCTCAGGCCAGGTCGGCGATGACCAGCTTGGCGATGTTGAAGTAGATCACCAGGCCGGTGCCGTCGATGAAGGTGGC
GATGAAGGGCGCCGACACCA

Product: two component system, transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MERATVLIVDDAPECLAALGELLQPHYRVLVANGGPAALRLAEGPIRPDLILLDVMMPEMDGREVFAGLRKREATAAIPV
VFVTAMNEWRDELLGLESGAVDYITKPIIPPLVLARVRTQLELKQARDRLSDQNRWLEVEVSRRMAENDLTQRMAIHALA
RLAEMRDLETGNHLLRTQGYVDALARRLRELPRYRGELSERRIELIARSAPLHDIGKVGVPDRILLKPGRLDADEWDIMR
SHARLGEQAIAQAERDIGHQVEFLSCAKQIAGHHHERWDGGGYPDGLAGERIPLPARLMALADVFDALISRRCYKAAMSF
EEARGLIAAGRGSQFQPELVDCFLDGFDDFAAIAARHADEPEAVAG

Sequences:

>Translated_366_residues
MERATVLIVDDAPECLAALGELLQPHYRVLVANGGPAALRLAEGPIRPDLILLDVMMPEMDGREVFAGLRKREATAAIPV
VFVTAMNEWRDELLGLESGAVDYITKPIIPPLVLARVRTQLELKQARDRLSDQNRWLEVEVSRRMAENDLTQRMAIHALA
RLAEMRDLETGNHLLRTQGYVDALARRLRELPRYRGELSERRIELIARSAPLHDIGKVGVPDRILLKPGRLDADEWDIMR
SHARLGEQAIAQAERDIGHQVEFLSCAKQIAGHHHERWDGGGYPDGLAGERIPLPARLMALADVFDALISRRCYKAAMSF
EEARGLIAAGRGSQFQPELVDCFLDGFDDFAAIAARHADEPEAVAG
>Mature_366_residues
MERATVLIVDDAPECLAALGELLQPHYRVLVANGGPAALRLAEGPIRPDLILLDVMMPEMDGREVFAGLRKREATAAIPV
VFVTAMNEWRDELLGLESGAVDYITKPIIPPLVLARVRTQLELKQARDRLSDQNRWLEVEVSRRMAENDLTQRMAIHALA
RLAEMRDLETGNHLLRTQGYVDALARRLRELPRYRGELSERRIELIARSAPLHDIGKVGVPDRILLKPGRLDADEWDIMR
SHARLGEQAIAQAERDIGHQVEFLSCAKQIAGHHHERWDGGGYPDGLAGERIPLPARLMALADVFDALISRRCYKAAMSF
EEARGLIAAGRGSQFQPELVDCFLDGFDDFAAIAARHADEPEAVAG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=135, Percent_Identity=40, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=37.2881355932203, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=122, Percent_Identity=40.9836065573771, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 40755; Mature: 40755

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERATVLIVDDAPECLAALGELLQPHYRVLVANGGPAALRLAEGPIRPDLILLDVMMPEM
CCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCEEEEEECCCCC
DGREVFAGLRKREATAAIPVVFVTAMNEWRDELLGLESGAVDYITKPIIPPLVLARVRTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHH
LELKQARDRLSDQNRWLEVEVSRRMAENDLTQRMAIHALARLAEMRDLETGNHLLRTQGY
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VDALARRLRELPRYRGELSERRIELIARSAPLHDIGKVGVPDRILLKPGRLDADEWDIMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCHHHHHHH
SHARLGEQAIAQAERDIGHQVEFLSCAKQIAGHHHERWDGGGYPDGLAGERIPLPARLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LADVFDALISRRCYKAAMSFEEARGLIAAGRGSQFQPELVDCFLDGFDDFAAIAARHADE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCC
PEAVAG
CCCCCC
>Mature Secondary Structure
MERATVLIVDDAPECLAALGELLQPHYRVLVANGGPAALRLAEGPIRPDLILLDVMMPEM
CCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCEEEEEECCCCC
DGREVFAGLRKREATAAIPVVFVTAMNEWRDELLGLESGAVDYITKPIIPPLVLARVRTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHH
LELKQARDRLSDQNRWLEVEVSRRMAENDLTQRMAIHALARLAEMRDLETGNHLLRTQGY
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VDALARRLRELPRYRGELSERRIELIARSAPLHDIGKVGVPDRILLKPGRLDADEWDIMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCHHHHHHH
SHARLGEQAIAQAERDIGHQVEFLSCAKQIAGHHHERWDGGGYPDGLAGERIPLPARLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LADVFDALISRRCYKAAMSFEEARGLIAAGRGSQFQPELVDCFLDGFDDFAAIAARHADE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCC
PEAVAG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]