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The map label for this gene is rpfG [H]

Identifier: 34498660

GI number: 34498660

Start: 3487831

End: 3488937

Strand: Reverse

Name: rpfG [H]

Synonym: CV_3205

Alternate gene names: 34498660

Gene position: 3488937-3487831 (Counterclockwise)

Preceding gene: 34498663

Following gene: 34498658

Centisome position: 73.43

GC content: 66.58

Gene sequence:

>1107_bases
ATGGCTACGCTGCAACTGATGCCGGAAATCATCGATCCGATCTCGTTTCAAGACTTTCGCGACGCCTTGTCCGATTACGC
GCCGCAACTGGAACAGCTGGTCTGCCAGCTGGAGGATGGCAGCGGCAACGAGGAAAAGATCGCCCAGCTGTTCCGCATCC
TCCACAGCGTCAAGGGCGACGCCAGCCTGTGCCAGGTGCACTTCGTGGAGCCCTATGTGCACGTGCTGGAGCACCTGCTG
GACAGGGTGAGGCACAAGGAGCTTCCTTATCTGGACGTGCTGGGCGACGTGCTGCTGCTGGTGATGGACCGGCTGGTGCT
GATGCTGGACGCCGTCGCGCAGGATTGGCCGCTCGACGACCTGCGGATGGACGCCTTGCGGATGCGGCTGGAGCCGCTGC
TGGACAAGAGCGCCGACGAGCTGCCCCAGGCTTGCGCCAGGCTGGTGGCCAGCATGATGGGACAGGCGCCTTCCTCCTCC
CAGGCTGCGGGCGAGCATGGCGCTAGCCATGGAGGCCATCGCTTCGCCGACCTGCAGTTTTTCCGCTCGCTGGCGGTGCA
GCTGGAGCAGCGCGTTCCGGCCTATGCCGGCCGCACCGAGCGCAATCTGGCGCTGGCGCTGGAAACCAACCGGCTGGCCG
AGCAGCCGCTGCCTATGGAGCAACTGGCGGCTGCCATCTACATGCACGATATCGGCATGATGCTGCTGCCGGAGTCCTTG
TGGCTGAAGGCCGGGCGGTTGAGCGAGGAGGACAGGCGGGCGCTGTCCGCGCATCCGGGCTGGGCGTCGGGATTGCTGGA
GCGGATGCCCGGTTGGGAGGAGGCCGCGGTGATGGTGGCCCAGCATCACGAACGTCCGGACGGCAGCGGCTATCCGGATG
GTCTGACGGACGGGGCCATCTGCTCGGGCGCCAGCCTGCTGGCGCTGGTGGACGCGTTCGAGTCGGTGATGCTCAAGCAC
GCCCAGCAAGGCCAGCGCCGTTCGATGCTGCGGGCGGTGGCCGAGCTGAACGCGTCGGAGCGGCAGTTCGACCCCTACTG
GCTGCAGCTGTTCAACCAGGTGGTGCGCACCCGGCTGGAGCTGGGCAATGTGGTCGGCGGCACCTAG

Upstream 100 bases:

>100_bases
TTGGACAGGATATTTACATTGGGTGGAACGACGCTTGCCGGGATGCGGAGCGGCCTGGCTTGCATATACTGGATAGGATG
CCAATCCGAAAGGACCCCAT

Downstream 100 bases:

>100_bases
CCGGGCCGCTCAGCGCGCGCCGTGGCGGGCGAACCAGTCCAGCATCCTGCGCCAGGCGTCGCCGGCGTCGCGCGGATGAT
AGCTGGGACGGTAGTCGGCG

Product: hypothetical protein

Products: ADP; phosphorylated ASP [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MATLQLMPEIIDPISFQDFRDALSDYAPQLEQLVCQLEDGSGNEEKIAQLFRILHSVKGDASLCQVHFVEPYVHVLEHLL
DRVRHKELPYLDVLGDVLLLVMDRLVLMLDAVAQDWPLDDLRMDALRMRLEPLLDKSADELPQACARLVASMMGQAPSSS
QAAGEHGASHGGHRFADLQFFRSLAVQLEQRVPAYAGRTERNLALALETNRLAEQPLPMEQLAAAIYMHDIGMMLLPESL
WLKAGRLSEEDRRALSAHPGWASGLLERMPGWEEAAVMVAQHHERPDGSGYPDGLTDGAICSGASLLALVDAFESVMLKH
AQQGQRRSMLRAVAELNASERQFDPYWLQLFNQVVRTRLELGNVVGGT

Sequences:

>Translated_368_residues
MATLQLMPEIIDPISFQDFRDALSDYAPQLEQLVCQLEDGSGNEEKIAQLFRILHSVKGDASLCQVHFVEPYVHVLEHLL
DRVRHKELPYLDVLGDVLLLVMDRLVLMLDAVAQDWPLDDLRMDALRMRLEPLLDKSADELPQACARLVASMMGQAPSSS
QAAGEHGASHGGHRFADLQFFRSLAVQLEQRVPAYAGRTERNLALALETNRLAEQPLPMEQLAAAIYMHDIGMMLLPESL
WLKAGRLSEEDRRALSAHPGWASGLLERMPGWEEAAVMVAQHHERPDGSGYPDGLTDGAICSGASLLALVDAFESVMLKH
AQQGQRRSMLRAVAELNASERQFDPYWLQLFNQVVRTRLELGNVVGGT
>Mature_367_residues
ATLQLMPEIIDPISFQDFRDALSDYAPQLEQLVCQLEDGSGNEEKIAQLFRILHSVKGDASLCQVHFVEPYVHVLEHLLD
RVRHKELPYLDVLGDVLLLVMDRLVLMLDAVAQDWPLDDLRMDALRMRLEPLLDKSADELPQACARLVASMMGQAPSSSQ
AAGEHGASHGGHRFADLQFFRSLAVQLEQRVPAYAGRTERNLALALETNRLAEQPLPMEQLAAAIYMHDIGMMLLPESLW
LKAGRLSEEDRRALSAHPGWASGLLERMPGWEEAAVMVAQHHERPDGSGYPDGLTDGAICSGASLLALVDAFESVMLKHA
QQGQRRSMLRAVAELNASERQFDPYWLQLFNQVVRTRLELGNVVGGT

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 40972; Mature: 40840

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50894 HPT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLQLMPEIIDPISFQDFRDALSDYAPQLEQLVCQLEDGSGNEEKIAQLFRILHSVKGD
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
ASLCQVHFVEPYVHVLEHLLDRVRHKELPYLDVLGDVLLLVMDRLVLMLDAVAQDWPLDD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LRMDALRMRLEPLLDKSADELPQACARLVASMMGQAPSSSQAAGEHGASHGGHRFADLQF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHH
FRSLAVQLEQRVPAYAGRTERNLALALETNRLAEQPLPMEQLAAAIYMHDIGMMLLPESL
HHHHHHHHHHHCCHHCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHH
WLKAGRLSEEDRRALSAHPGWASGLLERMPGWEEAAVMVAQHHERPDGSGYPDGLTDGAI
HHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
CSGASLLALVDAFESVMLKHAQQGQRRSMLRAVAELNASERQFDPYWLQLFNQVVRTRLE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LGNVVGGT
HHHCCCCC
>Mature Secondary Structure 
ATLQLMPEIIDPISFQDFRDALSDYAPQLEQLVCQLEDGSGNEEKIAQLFRILHSVKGD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
ASLCQVHFVEPYVHVLEHLLDRVRHKELPYLDVLGDVLLLVMDRLVLMLDAVAQDWPLDD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LRMDALRMRLEPLLDKSADELPQACARLVASMMGQAPSSSQAAGEHGASHGGHRFADLQF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHH
FRSLAVQLEQRVPAYAGRTERNLALALETNRLAEQPLPMEQLAAAIYMHDIGMMLLPESL
HHHHHHHHHHHCCHHCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHH
WLKAGRLSEEDRRALSAHPGWASGLLERMPGWEEAAVMVAQHHERPDGSGYPDGLTDGAI
HHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
CSGASLLALVDAFESVMLKHAQQGQRRSMLRAVAELNASERQFDPYWLQLFNQVVRTRLE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LGNVVGGT
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ASP [C]

Specific reaction: Catalyzes the hydrolysis of ATP to ADP and the phosphorylation of the 4-carboxyl group of an aspartic acid in chemotaxis proteins cheY and cheB [C]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with a carboxyl group as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]