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The map label for this gene is thiP [H]

Identifier: 34498617

GI number: 34498617

Start: 3445143

End: 3446708

Strand: Reverse

Name: thiP [H]

Synonym: CV_3162

Alternate gene names: 34498617

Gene position: 3446708-3445143 (Counterclockwise)

Preceding gene: 34498618

Following gene: 34498616

Centisome position: 72.55

GC content: 71.26

Gene sequence:

>1566_bases
TTGAAGCAGTCCGCCGTGCCCCGGCTGATCCTGGCCGGACTTCCGCTGTCCTTCCTCTTGCTGCTGGCCGTCGCGCCGCT
GTTCCGCCTGTTGGCGGAAGGCGGCCTGCAGTTCAATCTCTCGCTGCTGGCCGACGACTACCTGCGCTGGCGGCTGCTGT
GGTCGCTGATCCAGGCGTCGGCCAGTTGCCTGCTGTGCCTGGTTCTGGGCGTGCCGCTGGCCTGGATGCTGGCCCGTTAC
CGCTTTCCCGGACGCGCGTTGCTGCTGCGCTTGCTGATGCTGCCTTTCGTGATGCCGACGCTGGTGGCGGCGATGGGCGT
GCTGGCGTTGTTCGGGCCGCGGGGCGCGTTCGGCGTCAACCTGCAGGACACGCCGTGGCTGCTGCTGTACGGCAATCTGT
TCTTCAATCTGCCGCTGGTGGTGCGCGCCGGCTGCGACGGCTTTTCCCAGGTGCCGGCCAGCCGCCTGGCCGCCGCGCGC
ACGCTGGGCGCCACCCGCTGGCGCGCCTTCTGGCGCGTGGAATGGTCGGCGGCCCTGCCCTGGCTGCTGTCCAGCCTGTG
CCTGGTATTCCTGTACTGCTTCTCCGGCCTGGGCCTGGCGCTGGTGCTGGGCGGCCAGCGCTACGCGACGCTGGAGGTGG
AGATCTACACCCTGGTGGCTTACGAGCTGAACCTGGCCGACGCCGGCGCGCTGGCCTTGCTGGTATTGCTGGTGTGCGGC
GGCATCGCCGCAGTTTATGCCGCCTTGGCGCGCCGGCTGGCTACCGTGGCGAAGGTGGAGCCGCTGCCGCTGCGGCCGGC
TGCGAGCGGGATCGAGCGGCTGGCTTTGGGCGCGACTTTGTCCGCGCTGCTGCTGTGCAGCGGCCTGCCGCTGCTGGCGG
TGCTGTGGCGTGGCCTTTGCGCCGGCGCGGAATGGGCGGCGCTGTGGGACGAGGATACGCGCCAGGCCTTGTGGAACAGC
GCCCGCTTCACCGGCGTGACCGTGCTGGCGGCCGGCGCGCTGGGCCTGGCGCATGCGCTGGCCTGCGGCCGCAGCCTGGC
GTTGCGCGCCGCCGTCTTTCTGCCCTTCGTCGCTTCGCCGGTGTGCGTGGCTTTCGGCCTGCTGTTGCTGTACCCGCAAT
GGAGCGGCGCGCTGGGCCTGCTGATGGCGGCTTACGCGCTGCTGGCCTACCCGCTGGTGGCCAAGGCTGCGCTGGCGTCG
CTGGACGGCCAGCCGCCGCATTGGCGGGCCGCCGCCCGCGGCCTGGGCGCCGGCCCGTGGCGCGCTTTCCGCCGCGTCAC
CTGGCCCTTGCTGCAGCCTGCGCTGCGGCGCGGGCTGGCTTTCGCCGCCGCCACCGCGGTCGGCGAGTTCGCCGTCACGC
TGTTCCTGTCCCGGCCGGAGTGGCTGACGCTGACCACGCTGATCTACCAGCGGCTGGGGCGACCCGGCGAAGCCAATGCC
GACGCGGCGATGCAGCTCTCCTGCGCGCTGATGCTGCTGGCGCTCATTGCTTTCTGGATCATTGAAACCGCCGGCGGCGC
CGGCCCGGAGGAGGACAGCCGTGCTGACGCTGCGAAACCTGAATAA

Upstream 100 bases:

>100_bases
TGGCCGCCCAGCTTGGCTTCGCCAAGGTGGGCCAGGCGCGAGATGACGACGGTTGCGAAGATGTGCTGACGGCCGATTGG
CCATTGCCTGGAGCCTTTGT

Downstream 100 bases:

>100_bases
GCGCTTCGGCGCGCGCGTGGTGGCCGACGATGTGTCGCTGGAGGTGTCCGCCGGCGAAACGCTGGCGATGCTGGGGCCGT
CCGGCTGCGGCAAGAGCACG

Product: thiamine transport system permease

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MKQSAVPRLILAGLPLSFLLLLAVAPLFRLLAEGGLQFNLSLLADDYLRWRLLWSLIQASASCLLCLVLGVPLAWMLARY
RFPGRALLLRLLMLPFVMPTLVAAMGVLALFGPRGAFGVNLQDTPWLLLYGNLFFNLPLVVRAGCDGFSQVPASRLAAAR
TLGATRWRAFWRVEWSAALPWLLSSLCLVFLYCFSGLGLALVLGGQRYATLEVEIYTLVAYELNLADAGALALLVLLVCG
GIAAVYAALARRLATVAKVEPLPLRPAASGIERLALGATLSALLLCSGLPLLAVLWRGLCAGAEWAALWDEDTRQALWNS
ARFTGVTVLAAGALGLAHALACGRSLALRAAVFLPFVASPVCVAFGLLLLYPQWSGALGLLMAAYALLAYPLVAKAALAS
LDGQPPHWRAAARGLGAGPWRAFRRVTWPLLQPALRRGLAFAAATAVGEFAVTLFLSRPEWLTLTTLIYQRLGRPGEANA
DAAMQLSCALMLLALIAFWIIETAGGAGPEEDSRADAAKPE

Sequences:

>Translated_521_residues
MKQSAVPRLILAGLPLSFLLLLAVAPLFRLLAEGGLQFNLSLLADDYLRWRLLWSLIQASASCLLCLVLGVPLAWMLARY
RFPGRALLLRLLMLPFVMPTLVAAMGVLALFGPRGAFGVNLQDTPWLLLYGNLFFNLPLVVRAGCDGFSQVPASRLAAAR
TLGATRWRAFWRVEWSAALPWLLSSLCLVFLYCFSGLGLALVLGGQRYATLEVEIYTLVAYELNLADAGALALLVLLVCG
GIAAVYAALARRLATVAKVEPLPLRPAASGIERLALGATLSALLLCSGLPLLAVLWRGLCAGAEWAALWDEDTRQALWNS
ARFTGVTVLAAGALGLAHALACGRSLALRAAVFLPFVASPVCVAFGLLLLYPQWSGALGLLMAAYALLAYPLVAKAALAS
LDGQPPHWRAAARGLGAGPWRAFRRVTWPLLQPALRRGLAFAAATAVGEFAVTLFLSRPEWLTLTTLIYQRLGRPGEANA
DAAMQLSCALMLLALIAFWIIETAGGAGPEEDSRADAAKPE
>Mature_521_residues
MKQSAVPRLILAGLPLSFLLLLAVAPLFRLLAEGGLQFNLSLLADDYLRWRLLWSLIQASASCLLCLVLGVPLAWMLARY
RFPGRALLLRLLMLPFVMPTLVAAMGVLALFGPRGAFGVNLQDTPWLLLYGNLFFNLPLVVRAGCDGFSQVPASRLAAAR
TLGATRWRAFWRVEWSAALPWLLSSLCLVFLYCFSGLGLALVLGGQRYATLEVEIYTLVAYELNLADAGALALLVLLVCG
GIAAVYAALARRLATVAKVEPLPLRPAASGIERLALGATLSALLLCSGLPLLAVLWRGLCAGAEWAALWDEDTRQALWNS
ARFTGVTVLAAGALGLAHALACGRSLALRAAVFLPFVASPVCVAFGLLLLYPQWSGALGLLMAAYALLAYPLVAKAALAS
LDGQPPHWRAAARGLGAGPWRAFRRVTWPLLQPALRRGLAFAAATAVGEFAVTLFLSRPEWLTLTTLIYQRLGRPGEANA
DAAMQLSCALMLLALIAFWIIETAGGAGPEEDSRADAAKPE

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=465, Percent_Identity=28.6021505376344, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788764, Length=159, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082099, Length=164, Percent_Identity=30.4878048780488, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 55693; Mature: 55693

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSAVPRLILAGLPLSFLLLLAVAPLFRLLAEGGLQFNLSLLADDYLRWRLLWSLIQAS
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
ASCLLCLVLGVPLAWMLARYRFPGRALLLRLLMLPFVMPTLVAAMGVLALFGPRGAFGVN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LQDTPWLLLYGNLFFNLPLVVRAGCDGFSQVPASRLAAARTLGATRWRAFWRVEWSAALP
CCCCCEEEEECHHHHCCCHHEEECCCCHHHCCHHHHHHHHHHHHHHHHHHEEEEHHHHHH
WLLSSLCLVFLYCFSGLGLALVLGGQRYATLEVEIYTLVAYELNLADAGALALLVLLVCG
HHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHH
GIAAVYAALARRLATVAKVEPLPLRPAASGIERLALGATLSALLLCSGLPLLAVLWRGLC
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AGAEWAALWDEDTRQALWNSARFTGVTVLAAGALGLAHALACGRSLALRAAVFLPFVASP
CCCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCVAFGLLLLYPQWSGALGLLMAAYALLAYPLVAKAALASLDGQPPHWRAAARGLGAGPW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHH
RAFRRVTWPLLQPALRRGLAFAAATAVGEFAVTLFLSRPEWLTLTTLIYQRLGRPGEANA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCC
DAAMQLSCALMLLALIAFWIIETAGGAGPEEDSRADAAKPE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQSAVPRLILAGLPLSFLLLLAVAPLFRLLAEGGLQFNLSLLADDYLRWRLLWSLIQAS
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
ASCLLCLVLGVPLAWMLARYRFPGRALLLRLLMLPFVMPTLVAAMGVLALFGPRGAFGVN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LQDTPWLLLYGNLFFNLPLVVRAGCDGFSQVPASRLAAARTLGATRWRAFWRVEWSAALP
CCCCCEEEEECHHHHCCCHHEEECCCCHHHCCHHHHHHHHHHHHHHHHHHEEEEHHHHHH
WLLSSLCLVFLYCFSGLGLALVLGGQRYATLEVEIYTLVAYELNLADAGALALLVLLVCG
HHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHH
GIAAVYAALARRLATVAKVEPLPLRPAASGIERLALGATLSALLLCSGLPLLAVLWRGLC
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AGAEWAALWDEDTRQALWNSARFTGVTVLAAGALGLAHALACGRSLALRAAVFLPFVASP
CCCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCVAFGLLLLYPQWSGALGLLMAAYALLAYPLVAKAALASLDGQPPHWRAAARGLGAGPW
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHH
RAFRRVTWPLLQPALRRGLAFAAATAVGEFAVTLFLSRPEWLTLTTLIYQRLGRPGEANA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCC
DAAMQLSCALMLLALIAFWIIETAGGAGPEEDSRADAAKPE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]