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The map label for this gene is mdlC [H]

Identifier: 34498556

GI number: 34498556

Start: 3387614

End: 3389530

Strand: Direct

Name: mdlC [H]

Synonym: CV_3101

Alternate gene names: 34498556

Gene position: 3387614-3389530 (Clockwise)

Preceding gene: 34498555

Following gene: 34498557

Centisome position: 71.3

GC content: 66.98

Gene sequence:

>1917_bases
ATGAGCGTGGACGAGGCAGGGGGACAAAAGCGCTGGGCGCGCGATTACGTATTCGACATCCTGAGCCAGCTGGGCATACA
CCGGATTTTCGGCGTGCCCGGCACCAACGAGATTCCCATCATCGACGGCACCTCCTACCCGGAAAACCAGGTGGAGTACA
TCGAGTGCCTGCACGAGAACATCGCCATCGGCGCGGCGATGGGCTCGGCGCGGATGACCGGCAAGCCCGGCGTGCTGGTG
GTGCACGTGACGCCCGGCATCGCCCACGCCATCGGCAACCTGTTCAACGCCTGGCGCTCGCAAGCGCCGCTGGTGGTCCT
GTGCTGCCAGCAGCAGAACGAGCTGGTGACGCAGGAGCCGCTCTTGGCGTCCAACCTGGTGGACCTGGCCCGCCAGTACA
CCAAGTGGGCGCACGAGGTCCGCACCGAACAGGAATTCCCGATGGTGCTGCAACGCGCGTTCAAGGAGGCGATGGCGCCG
CCCAACGGCCCGGTTTTCGTCGCCATTCCCTGGGAGTTCACGATGCGCCGCATCGGCGACGACGACCGCATCAAGGGCGT
GACCCGGATCTCGCCGCACTTCACCGCCGACCGCCGGGCGATAGACCAGGCTGCGCAATTGCTGCGCGAAGCCCGGAACC
CCATCATCGTCACCGGCGACGCCGCCGGCTACGCCGAGGCCTGGCCCGAGCTGCAGCGGATGGCTGAGCTGCTGGGCGCG
CCGGTGCTGCAGCAGACCTTCAGCAGCCTGGCCAATTTCCCCAACAACGACTACCACTGGCAGGGCGAGCTGCCCGGCAG
CCAGTCCGGCGTTCAACAGGTGTTCGCCGGCCATGACGTCGCCTTCCTGTGCGGCTTCAGCAACCAGGCGCAGATCACGG
TCTACAAGTACAGCGACGGTCCGCTGATCCCGCCCGACGTCACCCAGGTCTACCTCAGCAACCACACCTGGGACATCGGC
AAGAACTACTACGGCGAAGCGGCGCTGTTCGGCGACCTGAAAGCCACGCTGCCGCTGATCAACCAGCTGATCGGCGACCA
GCCCTCGCCCGCAGCCGCCGCCCGCAACGCCAAGCTGAAGGAGATGGCCGCCCAGCGCAAGGTCGCCTGGGACGCCTACC
TGCGCGAGGCGATGCACCAGAACGAGATCTGGGCGGTGGTGATCGCCGACGCGCTGCGCAAGGAAATCGGCGAGCGCAAG
CTGGAGAAGCAGTTCGTCTACGTGCACGAGGCGGTGTCCGACCCGGCGCCGTTCCAGTACCTGCTGCCGTTCACCGACGA
GGCGGCGGCGCCGATCAGCTATTACTGCGTCGGCGGCGGCTCGCTGGGCTGGTCGATGCCGGCCACGCTGGGCATCAAGC
TGGAGCCCAGCGGCTGCCAGGACATCAATACCCGCTTCGTCGTCGCCGCCACCGGGGACGGCTCCTCGCTGTTCTACCCG
CAAACCTGGTGGACCGCGCAGCACCGCCAACTGGGCGTGCTCTACATCATCATCACCAACAACCATGAATACCACACGCT
GCAGATGGGGCTGACCCAGGTGGAGGCGATGTACGGCGAAGCGCCCGGCTACGAGTGGACAGCCAGGACGCAAGACCCGG
AATACCTGCGCATCCACCGGCCCAAGCCCGACTTCGTCACCCTGGCCAAGGCCTTCGGCGGCATGGAGGGCGAGATCGTC
CGCCACCCGGAGGACGTGCGCGCCGCGGTACGGCGCGGCATAGACCACGCGCTGAGCGGACACGCCTATGTGCTGGACAT
GCATACCGTCGGCCTGGACCAGCCGCCCCCCACGCCGGAGGACGCCAATGCCCGCTACGACAAGCAACCGCGCCTGGACT
GCTTCCATTTCGGCGCGGACGCGCAGCAACGCAATGACGGCGCCGGCCTGCCGGGCAACGTGCCGGTGATCTTCTGA

Upstream 100 bases:

>100_bases
GTGTGGAAATAAATCCGACAAGCTCCCTTCCATATCCTTCGTTTTCCCGCTGGATTACCACTCACGGTATCAATCCGAAT
TTTCCAGGGGGAGAACAAGC

Downstream 100 bases:

>100_bases
GCCCCATCCACTCCCGAGGAGGAGACCGCATCATGGCTGAAGAAACCTTCGACATCGCCATCGTCGGCGGCGGCGTATCC
GGCGTCTACTGCGCCTGGCG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSVDEAGGQKRWARDYVFDILSQLGIHRIFGVPGTNEIPIIDGTSYPENQVEYIECLHENIAIGAAMGSARMTGKPGVLV
VHVTPGIAHAIGNLFNAWRSQAPLVVLCCQQQNELVTQEPLLASNLVDLARQYTKWAHEVRTEQEFPMVLQRAFKEAMAP
PNGPVFVAIPWEFTMRRIGDDDRIKGVTRISPHFTADRRAIDQAAQLLREARNPIIVTGDAAGYAEAWPELQRMAELLGA
PVLQQTFSSLANFPNNDYHWQGELPGSQSGVQQVFAGHDVAFLCGFSNQAQITVYKYSDGPLIPPDVTQVYLSNHTWDIG
KNYYGEAALFGDLKATLPLINQLIGDQPSPAAAARNAKLKEMAAQRKVAWDAYLREAMHQNEIWAVVIADALRKEIGERK
LEKQFVYVHEAVSDPAPFQYLLPFTDEAAAPISYYCVGGGSLGWSMPATLGIKLEPSGCQDINTRFVVAATGDGSSLFYP
QTWWTAQHRQLGVLYIIITNNHEYHTLQMGLTQVEAMYGEAPGYEWTARTQDPEYLRIHRPKPDFVTLAKAFGGMEGEIV
RHPEDVRAAVRRGIDHALSGHAYVLDMHTVGLDQPPPTPEDANARYDKQPRLDCFHFGADAQQRNDGAGLPGNVPVIF

Sequences:

>Translated_638_residues
MSVDEAGGQKRWARDYVFDILSQLGIHRIFGVPGTNEIPIIDGTSYPENQVEYIECLHENIAIGAAMGSARMTGKPGVLV
VHVTPGIAHAIGNLFNAWRSQAPLVVLCCQQQNELVTQEPLLASNLVDLARQYTKWAHEVRTEQEFPMVLQRAFKEAMAP
PNGPVFVAIPWEFTMRRIGDDDRIKGVTRISPHFTADRRAIDQAAQLLREARNPIIVTGDAAGYAEAWPELQRMAELLGA
PVLQQTFSSLANFPNNDYHWQGELPGSQSGVQQVFAGHDVAFLCGFSNQAQITVYKYSDGPLIPPDVTQVYLSNHTWDIG
KNYYGEAALFGDLKATLPLINQLIGDQPSPAAAARNAKLKEMAAQRKVAWDAYLREAMHQNEIWAVVIADALRKEIGERK
LEKQFVYVHEAVSDPAPFQYLLPFTDEAAAPISYYCVGGGSLGWSMPATLGIKLEPSGCQDINTRFVVAATGDGSSLFYP
QTWWTAQHRQLGVLYIIITNNHEYHTLQMGLTQVEAMYGEAPGYEWTARTQDPEYLRIHRPKPDFVTLAKAFGGMEGEIV
RHPEDVRAAVRRGIDHALSGHAYVLDMHTVGLDQPPPTPEDANARYDKQPRLDCFHFGADAQQRNDGAGLPGNVPVIF
>Mature_637_residues
SVDEAGGQKRWARDYVFDILSQLGIHRIFGVPGTNEIPIIDGTSYPENQVEYIECLHENIAIGAAMGSARMTGKPGVLVV
HVTPGIAHAIGNLFNAWRSQAPLVVLCCQQQNELVTQEPLLASNLVDLARQYTKWAHEVRTEQEFPMVLQRAFKEAMAPP
NGPVFVAIPWEFTMRRIGDDDRIKGVTRISPHFTADRRAIDQAAQLLREARNPIIVTGDAAGYAEAWPELQRMAELLGAP
VLQQTFSSLANFPNNDYHWQGELPGSQSGVQQVFAGHDVAFLCGFSNQAQITVYKYSDGPLIPPDVTQVYLSNHTWDIGK
NYYGEAALFGDLKATLPLINQLIGDQPSPAAAARNAKLKEMAAQRKVAWDAYLREAMHQNEIWAVVIADALRKEIGERKL
EKQFVYVHEAVSDPAPFQYLLPFTDEAAAPISYYCVGGGSLGWSMPATLGIKLEPSGCQDINTRFVVAATGDGSSLFYPQ
TWWTAQHRQLGVLYIIITNNHEYHTLQMGLTQVEAMYGEAPGYEWTARTQDPEYLRIHRPKPDFVTLAKAFGGMEGEIVR
HPEDVRAAVRRGIDHALSGHAYVLDMHTVGLDQPPPTPEDANARYDKQPRLDCFHFGADAQQRNDGAGLPGNVPVIF

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=23.3449477351916, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI87081685, Length=598, Percent_Identity=23.9130434782609, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1786717, Length=501, Percent_Identity=25.5489021956088, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790104, Length=500, Percent_Identity=22.4, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1788716, Length=238, Percent_Identity=24.7899159663866, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787096, Length=341, Percent_Identity=23.7536656891496, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531299, Length=369, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=369, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=250, Percent_Identity=24.8, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320816, Length=237, Percent_Identity=22.7848101265823, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922626, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 70738; Mature: 70607

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDEAGGQKRWARDYVFDILSQLGIHRIFGVPGTNEIPIIDGTSYPENQVEYIECLHEN
CCCCCCCCCHHHHHHHHHHHHHHCCHHEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHH
IAIGAAMGSARMTGKPGVLVVHVTPGIAHAIGNLFNAWRSQAPLVVLCCQQQNELVTQEP
HEEEEECCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
LLASNLVDLARQYTKWAHEVRTEQEFPMVLQRAFKEAMAPPNGPVFVAIPWEFTMRRIGD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHCCC
DDRIKGVTRISPHFTADRRAIDQAAQLLREARNPIIVTGDAAGYAEAWPELQRMAELLGA
HHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCC
PVLQQTFSSLANFPNNDYHWQGELPGSQSGVQQVFAGHDVAFLCGFSNQAQITVYKYSDG
HHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCC
PLIPPDVTQVYLSNHTWDIGKNYYGEAALFGDLKATLPLINQLIGDQPSPAAAARNAKLK
CCCCCCHHEEEECCCCEECCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCHHHHHCCHHH
EMAAQRKVAWDAYLREAMHQNEIWAVVIADALRKEIGERKLEKQFVYVHEAVSDPAPFQY
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLPFTDEAAAPISYYCVGGGSLGWSMPATLGIKLEPSGCQDINTRFVVAATGDGSSLFYP
ECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEECC
QTWWTAQHRQLGVLYIIITNNHEYHTLQMGLTQVEAMYGEAPGYEWTARTQDPEYLRIHR
CCCCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEC
PKPDFVTLAKAFGGMEGEIVRHPEDVRAAVRRGIDHALSGHAYVLDMHTVGLDQPPPTPE
CCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHCHHHHHCCCEEEEEEEECCCCCCCCCCC
DANARYDKQPRLDCFHFGADAQQRNDGAGLPGNVPVIF
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEC
>Mature Secondary Structure 
SVDEAGGQKRWARDYVFDILSQLGIHRIFGVPGTNEIPIIDGTSYPENQVEYIECLHEN
CCCCCCCCHHHHHHHHHHHHHHCCHHEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHH
IAIGAAMGSARMTGKPGVLVVHVTPGIAHAIGNLFNAWRSQAPLVVLCCQQQNELVTQEP
HEEEEECCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
LLASNLVDLARQYTKWAHEVRTEQEFPMVLQRAFKEAMAPPNGPVFVAIPWEFTMRRIGD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHCCC
DDRIKGVTRISPHFTADRRAIDQAAQLLREARNPIIVTGDAAGYAEAWPELQRMAELLGA
HHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCC
PVLQQTFSSLANFPNNDYHWQGELPGSQSGVQQVFAGHDVAFLCGFSNQAQITVYKYSDG
HHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCC
PLIPPDVTQVYLSNHTWDIGKNYYGEAALFGDLKATLPLINQLIGDQPSPAAAARNAKLK
CCCCCCHHEEEECCCCEECCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCHHHHHCCHHH
EMAAQRKVAWDAYLREAMHQNEIWAVVIADALRKEIGERKLEKQFVYVHEAVSDPAPFQY
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLPFTDEAAAPISYYCVGGGSLGWSMPATLGIKLEPSGCQDINTRFVVAATGDGSSLFYP
ECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEECC
QTWWTAQHRQLGVLYIIITNNHEYHTLQMGLTQVEAMYGEAPGYEWTARTQDPEYLRIHR
CCCCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEC
PKPDFVTLAKAFGGMEGEIVRHPEDVRAAVRRGIDHALSGHAYVLDMHTVGLDQPPPTPE
CCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHCHHHHHCCCEEEEEEEECCCCCCCCCCC
DANARYDKQPRLDCFHFGADAQQRNDGAGLPGNVPVIF
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]