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The map label for this gene is gph2 [H]

Identifier: 34498547

GI number: 34498547

Start: 3377082

End: 3377729

Strand: Reverse

Name: gph2 [H]

Synonym: CV_3092

Alternate gene names: 34498547

Gene position: 3377729-3377082 (Counterclockwise)

Preceding gene: 34498548

Following gene: 34498545

Centisome position: 71.09

GC content: 64.2

Gene sequence:

>648_bases
ATGATCAAGGCAGTATTGTTCGATTTGGACGGCACGTTGGCCGACACCGCCCGCGACTTGGGCGCGGCGCTGAACCGCCT
GTTGGCGGAGGAGGGGCTAGCCCCTCTGCCTTACGAGCGTATCCGGCCCGTCGCCAGCCACGGCGCGCGCGGCCTGGTTC
AGCTCGGCTTTGGCGCAGACCTGCCCGCCGAGAGGATGGAGGCGCTGCGGGTGCGCTTCATGGATCTCTATGACGCCAAT
CTCGCGGGGGAAACCACGCTGTTCGATGGCGTCAACGAGCTGATCGCCGAACTGGACAAGCGCGGCCTGGCCTGGGGCAT
CATCACCAACAAGTCGATGCGCTTCACCGACCGCCTGGTGCCGTGGCTGCCTTTCGCCATCCCGCCGGCCGTGATCGTCA
GCGGCGATACCGTCGGCGTGGCCAAGCCCGATCCCAAGCCCATGCTGCATGCGACCGGGCAGATCGGCATCGCGCCGGAG
CAGTGCATGTATGTCGGCGACGCCGAGCGCGACATCCAGGCGGGTCGCAACGTCGGAATGAAAACGGTGTTGGTCAACTG
GGGATACTTCTCCGAACAGGATAGGCCTGAGCAGTGGGGGGCGGACATCGATATCGATCATCCCTTGCAGCTGCTTGATC
ATCTATAA

Upstream 100 bases:

>100_bases
TAGAGGAAACCGAAAATCCGGCCTATCGATTGTTCCTTGGCAAGACCATCCGTTGATGGTGCAATGTCGGCCTCATTCCC
AATCCGCTGAAGCAGGAATC

Downstream 100 bases:

>100_bases
AGACGAGTTTGGACGATACTGGAACAGGCCGGCGGCAACGCCGGCCTGTTTGTTTTCTGCCGTTGGCGCGGGGGTGAGGC
GGACAGGCATGCGCTTTGCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIKAVLFDLDGTLADTARDLGAALNRLLAEEGLAPLPYERIRPVASHGARGLVQLGFGADLPAERMEALRVRFMDLYDAN
LAGETTLFDGVNELIAELDKRGLAWGIITNKSMRFTDRLVPWLPFAIPPAVIVSGDTVGVAKPDPKPMLHATGQIGIAPE
QCMYVGDAERDIQAGRNVGMKTVLVNWGYFSEQDRPEQWGADIDIDHPLQLLDHL

Sequences:

>Translated_215_residues
MIKAVLFDLDGTLADTARDLGAALNRLLAEEGLAPLPYERIRPVASHGARGLVQLGFGADLPAERMEALRVRFMDLYDAN
LAGETTLFDGVNELIAELDKRGLAWGIITNKSMRFTDRLVPWLPFAIPPAVIVSGDTVGVAKPDPKPMLHATGQIGIAPE
QCMYVGDAERDIQAGRNVGMKTVLVNWGYFSEQDRPEQWGADIDIDHPLQLLDHL
>Mature_215_residues
MIKAVLFDLDGTLADTARDLGAALNRLLAEEGLAPLPYERIRPVASHGARGLVQLGFGADLPAERMEALRVRFMDLYDAN
LAGETTLFDGVNELIAELDKRGLAWGIITNKSMRFTDRLVPWLPFAIPPAVIVSGDTVGVAKPDPKPMLHATGQIGIAPE
QCMYVGDAERDIQAGRNVGMKTVLVNWGYFSEQDRPEQWGADIDIDHPLQLLDHL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=227, Percent_Identity=32.15859030837, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23555; Mature: 23555

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAVLFDLDGTLADTARDLGAALNRLLAEEGLAPLPYERIRPVASHGARGLVQLGFGAD
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHCCCCCEEEECCCCC
LPAERMEALRVRFMDLYDANLAGETTLFDGVNELIAELDKRGLAWGIITNKSMRFTDRLV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
PWLPFAIPPAVIVSGDTVGVAKPDPKPMLHATGQIGIAPEQCMYVGDAERDIQAGRNVGM
HHCCCCCCCEEEECCCEEECCCCCCCCCEEECCCCCCCHHHHEEECCCHHHHHHCCCCCE
KTVLVNWGYFSEQDRPEQWGADIDIDHPLQLLDHL
EEEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MIKAVLFDLDGTLADTARDLGAALNRLLAEEGLAPLPYERIRPVASHGARGLVQLGFGAD
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHCCCCCEEEECCCCC
LPAERMEALRVRFMDLYDANLAGETTLFDGVNELIAELDKRGLAWGIITNKSMRFTDRLV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
PWLPFAIPPAVIVSGDTVGVAKPDPKPMLHATGQIGIAPEQCMYVGDAERDIQAGRNVGM
HHCCCCCCCEEEECCCEEECCCCCCCCCEEECCCCCCCHHHHEEECCCHHHHHHCCCCCE
KTVLVNWGYFSEQDRPEQWGADIDIDHPLQLLDHL
EEEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]