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The map label for this gene is tyrS

Identifier: 34498527

GI number: 34498527

Start: 3349453

End: 3350727

Strand: Direct

Name: tyrS

Synonym: CV_3072

Alternate gene names: 34498527

Gene position: 3349453-3350727 (Clockwise)

Preceding gene: 34498525

Following gene: 34498535

Centisome position: 70.5

GC content: 63.84

Gene sequence:

>1275_bases
ATGACGCAAACCGCCCTTCTTCAGGATCTGGAGGCCCGTGGCCTCATCGCGCAAACCACCGATATGGCCGCGCTGCAAGA
ACTGCTGAACAAGGAATCGGTTACGCTTTATTGCGGCTTCGACCCGACGGCGGACAGCCTGCACATCGGCAGCCTGGTGC
CCATCCTGATGCTGAAGCGCTTCCAGCAGGCCGGCCACCGTCCGGTGGCCCTGGTGGGCGGCGCCACCGGCATGATAGGC
GACCCGAGCTTCAAGGCCGCCGAGCGCAAGCTGAACACCCCGGACGTGATCGAGGGCTGGGTGGAAAAGATCCGCAAGCA
AGTGGAACCCTTCCTGTCCTTCGAAGGCGAAAACGCCGCCGTGATGGCCAACAACTACGACTGGTTCGGCAAAATGAACG
CGCTCGAGTTCCTGCGCGACATCGGCAAGCACTTCTCTGTCAACGCGATGATCAAGAAGGAATCGGTGCAGCAGCGGATC
AACCGCGACGACCAGGGCATCTCCTACACCGAGTTCTCCTACAGCCTGCTGCAGGGCTATGATTTCGCCGAGCTGAACCA
GCGGCTGGGCTGCAAGCTGCAGATCGGCGGTTCCGACCAGTGGGGCAACATCACCGCCGGCACCGACCTGACCCGCCGCC
TGAACCAGACCCAGGTATACGGCCTGACCATGCCGCTGGTGACCAAGGCCGACGGCACCAAATTCGGCAAGACCGAATCC
GGCACCATCTGGCTAGACGCGAAGAAGACCTCGCCCTACGCCTTCTACCAGTTCTGGCTCGGCACGGCCGACGCCGACGT
GTACAAGTTCCTGCGCTACTTCAGCTTCCTGTCGGTGGACGAGATCGCCTCGATCGAAGAAGCCGACAAGAGCCGCGAAG
GCAAGCCGGAAGGCCAGCGCATCCTGGCCGAGCAAGTCACCGAGCTGGTGCACGGCAAGGCCGCGCTGGAAGCCGCGCAG
CGCATCACCCACAGCCTGTTCAGCAACGACCTGACCAACCTGACCGCCGACGACTTCGCCCAGCTGGCGCAGGACGGCCT
GCCGACCATCAAGCTGGACAAGAGCGCCAGCGGCCTGATCGACGCGCTGGCCGCCGGCGGCCTGGCCAAGTCCAAGAGCG
AGGCGCGCACCTTCATCCAGAGCGGCGCCGTCAGCGTCAACGGCATCAAGGTGGACAGCCTGGAGCACGCGATCGGCGAC
GGCGAGCGCCTGTTCGGCCAGTACTCGCTGCTGAAGCGCGGTAAGAAGCTGTACGCGCTGGTCGACTGGCAGTAA

Upstream 100 bases:

>100_bases
ATGGACGTTTTTCCCGACGACGCGGCGCGGCGTCGGGCCCGCATGTCCCTGACGGCCCCCGAGGGCCGTTGTTTTTCTAC
CGTGTTGGAGACCCAGTCAA

Downstream 100 bases:

>100_bases
ACGCCCCGCGCAAACAAAAAGCCCGCGAAAGCGGGCTTTTTCATCGCCAATCCAACCTCGCGATGCGCCTGAGCGAAGAC
GCGCCGGCACCGTCGCCAAG

Product: tyrosine-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTQTALLQDLEARGLIAQTTDMAALQELLNKESVTLYCGFDPTADSLHIGSLVPILMLKRFQQAGHRPVALVGGATGMIG
DPSFKAAERKLNTPDVIEGWVEKIRKQVEPFLSFEGENAAVMANNYDWFGKMNALEFLRDIGKHFSVNAMIKKESVQQRI
NRDDQGISYTEFSYSLLQGYDFAELNQRLGCKLQIGGSDQWGNITAGTDLTRRLNQTQVYGLTMPLVTKADGTKFGKTES
GTIWLDAKKTSPYAFYQFWLGTADADVYKFLRYFSFLSVDEIASIEEADKSREGKPEGQRILAEQVTELVHGKAALEAAQ
RITHSLFSNDLTNLTADDFAQLAQDGLPTIKLDKSASGLIDALAAGGLAKSKSEARTFIQSGAVSVNGIKVDSLEHAIGD
GERLFGQYSLLKRGKKLYALVDWQ

Sequences:

>Translated_424_residues
MTQTALLQDLEARGLIAQTTDMAALQELLNKESVTLYCGFDPTADSLHIGSLVPILMLKRFQQAGHRPVALVGGATGMIG
DPSFKAAERKLNTPDVIEGWVEKIRKQVEPFLSFEGENAAVMANNYDWFGKMNALEFLRDIGKHFSVNAMIKKESVQQRI
NRDDQGISYTEFSYSLLQGYDFAELNQRLGCKLQIGGSDQWGNITAGTDLTRRLNQTQVYGLTMPLVTKADGTKFGKTES
GTIWLDAKKTSPYAFYQFWLGTADADVYKFLRYFSFLSVDEIASIEEADKSREGKPEGQRILAEQVTELVHGKAALEAAQ
RITHSLFSNDLTNLTADDFAQLAQDGLPTIKLDKSASGLIDALAAGGLAKSKSEARTFIQSGAVSVNGIKVDSLEHAIGD
GERLFGQYSLLKRGKKLYALVDWQ
>Mature_423_residues
TQTALLQDLEARGLIAQTTDMAALQELLNKESVTLYCGFDPTADSLHIGSLVPILMLKRFQQAGHRPVALVGGATGMIGD
PSFKAAERKLNTPDVIEGWVEKIRKQVEPFLSFEGENAAVMANNYDWFGKMNALEFLRDIGKHFSVNAMIKKESVQQRIN
RDDQGISYTEFSYSLLQGYDFAELNQRLGCKLQIGGSDQWGNITAGTDLTRRLNQTQVYGLTMPLVTKADGTKFGKTESG
TIWLDAKKTSPYAFYQFWLGTADADVYKFLRYFSFLSVDEIASIEEADKSREGKPEGQRILAEQVTELVHGKAALEAAQR
ITHSLFSNDLTNLTADDFAQLAQDGLPTIKLDKSASGLIDALAAGGLAKSKSEARTFIQSGAVSVNGIKVDSLEHAIGDG
ERLFGQYSLLKRGKKLYALVDWQ

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=439, Percent_Identity=34.1685649202733, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI1787925, Length=425, Percent_Identity=63.2941176470588, Blast_Score=574, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25145802, Length=436, Percent_Identity=36.9266055045872, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI25145805, Length=313, Percent_Identity=35.7827476038339, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325160, Length=401, Percent_Identity=34.6633416458853, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI20130383, Length=446, Percent_Identity=35.4260089686099, Blast_Score=285, Evalue=5e-77,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CHRVO (Q7NTI2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902742.1
- ProteinModelPortal:   Q7NTI2
- SMR:   Q7NTI2
- GeneID:   2548686
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3072
- NMPDR:   fig|243365.1.peg.3072
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- PhylomeDB:   Q7NTI2
- ProtClustDB:   PRK05912
- BioCyc:   CVIO243365:CV_3072-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46718; Mature: 46587

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 37-37 BINDING 174-174 BINDING 178-178 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTALLQDLEARGLIAQTTDMAALQELLNKESVTLYCGFDPTADSLHIGSLVPILMLKR
CCHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHH
FQQAGHRPVALVGGATGMIGDPSFKAAERKLNTPDVIEGWVEKIRKQVEPFLSFEGENAA
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCEE
VMANNYDWFGKMNALEFLRDIGKHFSVNAMIKKESVQQRINRDDQGISYTEFSYSLLQGY
EEECCCCHHCCHHHHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCC
DFAELNQRLGCKLQIGGSDQWGNITAGTDLTRRLNQTQVYGLTMPLVTKADGTKFGKTES
CHHHHHHHCCCEEEECCCCCCCCEECCCHHHHHHCCCEEEEEEECEEECCCCCCCCCCCC
GTIWLDAKKTSPYAFYQFWLGTADADVYKFLRYFSFLSVDEIASIEEADKSREGKPEGQR
CEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
ILAEQVTELVHGKAALEAAQRITHSLFSNDLTNLTADDFAQLAQDGLPTIKLDKSASGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCHHHHH
DALAAGGLAKSKSEARTFIQSGAVSVNGIKVDSLEHAIGDGERLFGQYSLLKRGKKLYAL
HHHHHCCCCCCHHHHHHHHHHCCEEECCEEECHHHHHHCCCHHHHHHHHHHHCCCEEEEE
VDWQ
ECCC
>Mature Secondary Structure 
TQTALLQDLEARGLIAQTTDMAALQELLNKESVTLYCGFDPTADSLHIGSLVPILMLKR
CHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHHH
FQQAGHRPVALVGGATGMIGDPSFKAAERKLNTPDVIEGWVEKIRKQVEPFLSFEGENAA
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCEE
VMANNYDWFGKMNALEFLRDIGKHFSVNAMIKKESVQQRINRDDQGISYTEFSYSLLQGY
EEECCCCHHCCHHHHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCC
DFAELNQRLGCKLQIGGSDQWGNITAGTDLTRRLNQTQVYGLTMPLVTKADGTKFGKTES
CHHHHHHHCCCEEEECCCCCCCCEECCCHHHHHHCCCEEEEEEECEEECCCCCCCCCCCC
GTIWLDAKKTSPYAFYQFWLGTADADVYKFLRYFSFLSVDEIASIEEADKSREGKPEGQR
CEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
ILAEQVTELVHGKAALEAAQRITHSLFSNDLTNLTADDFAQLAQDGLPTIKLDKSASGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCHHHHH
DALAAGGLAKSKSEARTFIQSGAVSVNGIKVDSLEHAIGDGERLFGQYSLLKRGKKLYAL
HHHHHCCCCCCHHHHHHHHHHCCEEECCEEECHHHHHHCCCHHHHHHHHHHHCCCEEEEE
VDWQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782