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The map label for this gene is yfcF [H]

Identifier: 34498479

GI number: 34498479

Start: 3301143

End: 3301754

Strand: Reverse

Name: yfcF [H]

Synonym: CV_3024

Alternate gene names: 34498479

Gene position: 3301754-3301143 (Counterclockwise)

Preceding gene: 34498480

Following gene: 34498478

Centisome position: 69.49

GC content: 69.12

Gene sequence:

>612_bases
ATGCTGAAACTCTATGTCGATCACGATTTTTTCAGCCCCTACGCGATGGCCGCCTTCGTCGCGCTGAGCGAAAAGGGCCT
GCCCTTCGAAACGCTGACCCGCGATCTGGCAGCGGGCCAGCAGTTCGAGCCCGGCTACCGCTCGCAATCGCTGGCTTCCC
GCGTGCCGATGCTGTTGGACGGCGATTTCGCGCTCAGCGAATCGTCGGCCATCATCGAATATCTGGAAGACGCCTATCCC
GACACCGTCCGCGTGTTGCCGGCCGATCCAAAAGCCCGCGCCCGCGCGCGCCAGCTGCAGGCGTGGCTGCGCAGCGACCT
GCTGCCGCTGCGCGACGAACGCAGCACCGAGACGGTGTTCGGCCGCAAGCCGGCTTTGCCGCTGGGCGAGGCGGCGCGCA
AGGCGGCGGACAAGCTGATCGCGGTGGCCGAGGCGGTGGCGCCGGCGGACGGCGGCGATCTGTTCGGCGCCTGGTGCGTG
GCGGACGCCGAGCTGGCCCTGCTGCTGAAGCGGCTGACGGCGGACGAGTTGCCGCCGTGGTTGTCCGCTTACGCCGAAAG
GCAATGGCAGCGGCCGTCGCTGGCCGCTTGGCGCGGCCGGCAGGCTCGCTGA

Upstream 100 bases:

>100_bases
ACTGGATTGTTCGATCCTGCTTGACGCCCGGTGCTCCGCCGCCGGCTTGGCAGCGGACGCTTCGGCGATTAGCATGGCGT
TTTCCCATCAAGATGCCGCC

Downstream 100 bases:

>100_bases
TCCGGCCCGACTCTCTCTATACTGCCGGTAAAGAAGAGCGGGGACGGAGACGATGAGGGGTGGGCAAACGAACCTCCGGC
TATGGGCCGGCCTGCTGCTG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLKLYVDHDFFSPYAMAAFVALSEKGLPFETLTRDLAAGQQFEPGYRSQSLASRVPMLLDGDFALSESSAIIEYLEDAYP
DTVRVLPADPKARARARQLQAWLRSDLLPLRDERSTETVFGRKPALPLGEAARKAADKLIAVAEAVAPADGGDLFGAWCV
ADAELALLLKRLTADELPPWLSAYAERQWQRPSLAAWRGRQAR

Sequences:

>Translated_203_residues
MLKLYVDHDFFSPYAMAAFVALSEKGLPFETLTRDLAAGQQFEPGYRSQSLASRVPMLLDGDFALSESSAIIEYLEDAYP
DTVRVLPADPKARARARQLQAWLRSDLLPLRDERSTETVFGRKPALPLGEAARKAADKLIAVAEAVAPADGGDLFGAWCV
ADAELALLLKRLTADELPPWLSAYAERQWQRPSLAAWRGRQAR
>Mature_203_residues
MLKLYVDHDFFSPYAMAAFVALSEKGLPFETLTRDLAAGQQFEPGYRSQSLASRVPMLLDGDFALSESSAIIEYLEDAYP
DTVRVLPADPKARARARQLQAWLRSDLLPLRDERSTETVFGRKPALPLGEAARKAADKLIAVAEAVAPADGGDLFGAWCV
ADAELALLLKRLTADELPPWLSAYAERQWQRPSLAAWRGRQAR

Specific function: Unknown

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788639, Length=197, Percent_Identity=48.2233502538071, Blast_Score=177, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 22461; Mature: 22461

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLYVDHDFFSPYAMAAFVALSEKGLPFETLTRDLAAGQQFEPGYRSQSLASRVPMLLD
CEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEC
GDFALSESSAIIEYLEDAYPDTVRVLPADPKARARARQLQAWLRSDLLPLRDERSTETVF
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHC
GRKPALPLGEAARKAADKLIAVAEAVAPADGGDLFGAWCVADAELALLLKRLTADELPPW
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LSAYAERQWQRPSLAAWRGRQAR
HHHHHHHHCCCCCHHHHCCCCCC
>Mature Secondary Structure
MLKLYVDHDFFSPYAMAAFVALSEKGLPFETLTRDLAAGQQFEPGYRSQSLASRVPMLLD
CEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEC
GDFALSESSAIIEYLEDAYPDTVRVLPADPKARARARQLQAWLRSDLLPLRDERSTETVF
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHC
GRKPALPLGEAARKAADKLIAVAEAVAPADGGDLFGAWCVADAELALLLKRLTADELPPW
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LSAYAERQWQRPSLAAWRGRQAR
HHHHHHHHCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]