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The map label for this gene is fliA [H]

Identifier: 34498445

GI number: 34498445

Start: 3266878

End: 3267573

Strand: Reverse

Name: fliA [H]

Synonym: CV_2990

Alternate gene names: 34498445

Gene position: 3267573-3266878 (Counterclockwise)

Preceding gene: 34498446

Following gene: 34498444

Centisome position: 68.78

GC content: 70.11

Gene sequence:

>696_bases
ATGTACGCCGAGTGCTACGAGCAGCCGCCCCAAGCCGCCGACGAGGCGCGGCACCTGGCCGTCTATGCGCCGCTGGTCAA
GCGCGCCGCGCGCCAGCTCTCCAGCCAGGCCGGCGGCGTGTTCGATCGCGAGGACATGGAGCAGATCGGCCTGATGGCCT
TGCTCGATTGCCTGCGCCGCTATGGCGGCGAGCCGGACGAGCGCTTCGCCGGCTTCGCCATGCTGCGCATCCGCGGCGCG
ATCCTGGACGAGCTGCGGCGGCTGGACTGGCGCCCGCGCTCGGTGCGGCAGGAGGCGCACCGGGTGCGCGACGGCTTGCG
CGAATTGCGGCGCAAGCTCAGGCGAGAGCCGACCGAGGCGGAGGCGCTGGGGCTGCTGGGGCTGGACGCCGACGCCTACC
GCGATGCCCTGTTGGCGGAAAACGCCGAGGCGATGGCCAGCTTCGACGAGCTGGTGGCTTCGGGGATGGAGCTGGCGGCC
GAAGGCACGCCCGAGGCGAGGCTGGAGCGGCAAAGCGAGCTGGCGCGCGCGCTGACCGCGCTCAACGAGCGCGAGCAGCA
GGTGATCCAGCTGTATTACGAGTTCGAACTGAGTCTCAAGGAGATCGCGGCGGTGCTGGAGCTGACCGAGGCCCGCATCT
GCCAGATCAACAAGAGCGCCCTGAAAAAGATGCAGGCCTGCCTGATGGCGGCCTGA

Upstream 100 bases:

>100_bases
AGCTGCTGGACCGTTCCTACCGCGCGACTTTCGCGCGCGAGTCGCGCGAGCAGCCGTTCACCGAGGTGATGGTCAGCAAG
CTGATCATCGAGTAAGCGCG

Downstream 100 bases:

>100_bases
AGCAAGGAAACACTCCATGCAGCAATTGTTTGGCATTCTGATCGTGCTGGTTTGCGTATTCGGCGGCTTCATGCTGCACG
GCGGCGTGCTGTCCATCATC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MYAECYEQPPQAADEARHLAVYAPLVKRAARQLSSQAGGVFDREDMEQIGLMALLDCLRRYGGEPDERFAGFAMLRIRGA
ILDELRRLDWRPRSVRQEAHRVRDGLRELRRKLRREPTEAEALGLLGLDADAYRDALLAENAEAMASFDELVASGMELAA
EGTPEARLERQSELARALTALNEREQQVIQLYYEFELSLKEIAAVLELTEARICQINKSALKKMQACLMAA

Sequences:

>Translated_231_residues
MYAECYEQPPQAADEARHLAVYAPLVKRAARQLSSQAGGVFDREDMEQIGLMALLDCLRRYGGEPDERFAGFAMLRIRGA
ILDELRRLDWRPRSVRQEAHRVRDGLRELRRKLRREPTEAEALGLLGLDADAYRDALLAENAEAMASFDELVASGMELAA
EGTPEARLERQSELARALTALNEREQQVIQLYYEFELSLKEIAAVLELTEARICQINKSALKKMQACLMAA
>Mature_231_residues
MYAECYEQPPQAADEARHLAVYAPLVKRAARQLSSQAGGVFDREDMEQIGLMALLDCLRRYGGEPDERFAGFAMLRIRGA
ILDELRRLDWRPRSVRQEAHRVRDGLRELRRKLRREPTEAEALGLLGLDADAYRDALLAENAEAMASFDELVASGMELAA
EGTPEARLERQSELARALTALNEREQQVIQLYYEFELSLKEIAAVLELTEARICQINKSALKKMQACLMAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=36.8663594470046, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26096; Mature: 26096

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAECYEQPPQAADEARHLAVYAPLVKRAARQLSSQAGGVFDREDMEQIGLMALLDCLRR
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YGGEPDERFAGFAMLRIRGAILDELRRLDWRPRSVRQEAHRVRDGLRELRRKLRREPTEA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EALGLLGLDADAYRDALLAENAEAMASFDELVASGMELAAEGTPEARLERQSELARALTA
HHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LNEREQQVIQLYYEFELSLKEIAAVLELTEARICQINKSALKKMQACLMAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MYAECYEQPPQAADEARHLAVYAPLVKRAARQLSSQAGGVFDREDMEQIGLMALLDCLRR
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YGGEPDERFAGFAMLRIRGAILDELRRLDWRPRSVRQEAHRVRDGLRELRRKLRREPTEA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EALGLLGLDADAYRDALLAENAEAMASFDELVASGMELAAEGTPEARLERQSELARALTA
HHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LNEREQQVIQLYYEFELSLKEIAAVLELTEARICQINKSALKKMQACLMAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]