Click here to switch to the map view.

The map label for this gene is flgJ [H]

Identifier: 34498439

GI number: 34498439

Start: 3262599

End: 3263237

Strand: Reverse

Name: flgJ [H]

Synonym: CV_2984

Alternate gene names: 34498439

Gene position: 3263237-3262599 (Counterclockwise)

Preceding gene: 34498440

Following gene: 34498438

Centisome position: 68.68

GC content: 69.95

Gene sequence:

>639_bases
CTGGATCTCCCATCCGAGGGCGCGGGTTTTGCCGAGCAGTTTGCCGGCGTGCGCGCCGAGGTGGAGCGCTTCATCGCCGA
CGGCGGATCGGCTTCGGCGCAGCAATTGTCGCCGGAGGCCTGGCGGGTGCGGCAGCAGCTGGGCGCCGGTTCGGTCGACT
TGTCTGCAGTGCCGCCGGACAGGCAGGCTTTTGTCGAGGAAATGCTGCCGCACGCGCAGGCGGCGGCGGCCAGGCTGGGC
GTGACGCCCGATCTGGTGCTGGCCCATGCGGCGCTGGAGTCGGGTTGGGGGCGCAAGCCGCTGACGCGTGGCGACGGCGG
CAACAGCCATAACCTGTTCGGCATCAAGGCCGACGCGCGCTGGCGCGGCGAGGTGGCCGCCTCGCTCACCACCGAATTCA
TCCATGGCCAGAAGCAGAGCAGGGTGGAGTCGTTCCGCGCCTATCCGGATTACCGCGCGGCATTCGACGATTACGCCGAT
CTCCTGGCGTCGAATCCGCGCTACCGCGCGGCGCTGGGCGTTGGCGGCGACGCCAGGGCCTTCGCCGAGGCCTTGGCGCG
CGGAGGCTATGCGACCGATCCCGACTATGCCGGCAAGTTGGCCGGTTTGGCGGGGCAGTTCCGCAAGGGCATGCGGTGA

Upstream 100 bases:

>100_bases
CTGCAGCGCGTGCTCGAGGGCGAAAGCGATGTTTACGCAGCGCAGTGAGGGCTGGCAGGCTGCTGGCGCCGCGCAGCCGT
CGGACGCGGTGGCGCTGCCG

Downstream 100 bases:

>100_bases
GCGGCGAGTCAGTAAGGTTGGCACCATGGGCGCCAATTTTCATTTGTGCCGTTTTTTGAAAAATAAAAAGGAAAAAGCCG
AAATCTTGCGATTTCGGCTT

Product: flagellar protein flgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDLPSEGAGFAEQFAGVRAEVERFIADGGSASAQQLSPEAWRVRQQLGAGSVDLSAVPPDRQAFVEEMLPHAQAAAARLG
VTPDLVLAHAALESGWGRKPLTRGDGGNSHNLFGIKADARWRGEVAASLTTEFIHGQKQSRVESFRAYPDYRAAFDDYAD
LLASNPRYRAALGVGGDARAFAEALARGGYATDPDYAGKLAGLAGQFRKGMR

Sequences:

>Translated_212_residues
MDLPSEGAGFAEQFAGVRAEVERFIADGGSASAQQLSPEAWRVRQQLGAGSVDLSAVPPDRQAFVEEMLPHAQAAAARLG
VTPDLVLAHAALESGWGRKPLTRGDGGNSHNLFGIKADARWRGEVAASLTTEFIHGQKQSRVESFRAYPDYRAAFDDYAD
LLASNPRYRAALGVGGDARAFAEALARGGYATDPDYAGKLAGLAGQFRKGMR
>Mature_212_residues
MDLPSEGAGFAEQFAGVRAEVERFIADGGSASAQQLSPEAWRVRQQLGAGSVDLSAVPPDRQAFVEEMLPHAQAAAARLG
VTPDLVLAHAALESGWGRKPLTRGDGGNSHNLFGIKADARWRGEVAASLTTEFIHGQKQSRVESFRAYPDYRAAFDDYAD
LLASNPRYRAALGVGGDARAFAEALARGGYATDPDYAGKLAGLAGQFRKGMR

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=153, Percent_Identity=43.1372549019608, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 22549; Mature: 22549

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPSEGAGFAEQFAGVRAEVERFIADGGSASAQQLSPEAWRVRQQLGAGSVDLSAVPPD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCC
RQAFVEEMLPHAQAAAARLGVTPDLVLAHAALESGWGRKPLTRGDGGNSHNLFGIKADAR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCC
WRGEVAASLTTEFIHGQKQSRVESFRAYPDYRAAFDDYADLLASNPRYRAALGVGGDARA
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHH
FAEALARGGYATDPDYAGKLAGLAGQFRKGMR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLPSEGAGFAEQFAGVRAEVERFIADGGSASAQQLSPEAWRVRQQLGAGSVDLSAVPPD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCC
RQAFVEEMLPHAQAAAARLGVTPDLVLAHAALESGWGRKPLTRGDGGNSHNLFGIKADAR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCC
WRGEVAASLTTEFIHGQKQSRVESFRAYPDYRAAFDDYADLLASNPRYRAALGVGGDARA
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHH
FAEALARGGYATDPDYAGKLAGLAGQFRKGMR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]