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The map label for this gene is radA [H]

Identifier: 34498429

GI number: 34498429

Start: 3251353

End: 3252714

Strand: Reverse

Name: radA [H]

Synonym: CV_2974

Alternate gene names: 34498429

Gene position: 3252714-3251353 (Counterclockwise)

Preceding gene: 34498430

Following gene: 34498428

Centisome position: 68.46

GC content: 66.96

Gene sequence:

>1362_bases
ATGGCCAAGAACAAAACCGTATTCAGCTGCACCGAGTGCGGCGGACAGTCGCCCAAGTGGCAGGGCCAGTGCCCGCACTG
CAGCGCCTGGAACACGCTGGTGGAGGCGGTGGCCGCGCCGGCCGCCGCCAGTCCCCGCTTCCAGTCCTGGGCGGCCAACG
TCACCAAGGTGCAGAAACTGTCCGAGGTGCAGACCGAGGAAACCCCGCGCGATCCGTCCGGCATCGACGAACTGGACCGC
GTGCTGGGCGGCGGCATCGTGCGCGGCGCGGTGGTGCTGATCGGCGGCGACCCGGGCATCGGCAAGTCCACGCTGCTGCT
GCAGGCCTTGTCTCAGATCGGCCGCAGCCGCAAGGTGCTCTACGTTTCCGGCGAGGAATCGGCGCAGCAGATCGCCTTGC
GCGCCTCGCGACTGGCGCTGGATACCAGCAGCGTCGATCTGCTGGCCGAGATCTGCATCGAGAACATTCTGGAAACGCTG
AAGCGAGAGCAGCCGGAAGTGGTGGTGATCGACTCGATCCAGACGCTTTACACCGAGCAGGTGACGTCGGCGCCGGGCTC
GGTGTCGCAGGTGCGCGAGTGCGCGGCGCAGCTGACGCGGATGGCCAAGCAGAGCGGCATCACCGTGTTGCTGGTCGGCC
ATGTGACCAAGGAAGGTTCGCTGGCCGGTCCGCGCGTGCTGGAGCACATGGTGGACACCGTGCTGTATTTCGAGGGCGAC
TCCCACTCCAGCTACCGGATGATACGCGCGATCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCGATGAC
CGACCGCGGCCTGAAGGGCGTGTCCAATCCGTCGGCCATCTTCCTTTCCTCGTATCGCGACGACGTCGCCGGCTCCTGCG
TGCTGGTGACGCAGGAAGGCACGCGGCCGCTGCTGGTGGAAATCCAGGCCTTGGTCGACGACTGCCACGGCTTCCAGCCC
AAGCGTCTCACCGTCGGCCTGGAGCAGAACCGTCTGTCCATGCTGCTGGCGGTGCTGCACCGCCACGGCGGCGTCGCCTG
TTTCGACCAGGACGTGTTCCTCAACGCGGTGGGCGGCGTCAAGATCAACGAGCCGGCCGCCGACCTCGCCATCATCCTGG
CCATGGTTTCGTCGCTGCGCAACAAGGCGCTGCCGGAAAAGCTGGTGGTGTTCGGCGAGGTGGGCCTGGCCGGCGAGGTG
CGGCCGGTGACGCGCGGCCAGGAACGCTTGAAGGAGGCGGCCAAGCTGGGCTTCACCCGCGCCATCGTGCCCACCGCCAA
CAAGCCGCGGCAGGAAATCGAGGGCCTGAAGGTCTTGGCCGTGGACCGCCTGGATCAGGCGGTGGAATACTGCAGGGAGT
AG

Upstream 100 bases:

>100_bases
CCGCGGCCGGGCGCGGAGCCGGCGCCAAGGCGTTTTTTGACTGCCGGTTTCGCGCCGTCCTGCTATCCTTCCGCCTTTGG
TACCCCCTGATTTTTCTCCG

Downstream 100 bases:

>100_bases
CGCATGACGGTCGATCCGCAGGCGATAGAACAAGAACGTCCTTATCTGCTGCGCTACGCGCTGGCCCAGCTGCGCGAGCG
CGACGCCGCCGAGGAGGCAG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAKNKTVFSCTECGGQSPKWQGQCPHCSAWNTLVEAVAAPAAASPRFQSWAANVTKVQKLSEVQTEETPRDPSGIDELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGRSRKVLYVSGEESAQQIALRASRLALDTSSVDLLAEICIENILETL
KREQPEVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPTANKPRQEIEGLKVLAVDRLDQAVEYCRE

Sequences:

>Translated_453_residues
MAKNKTVFSCTECGGQSPKWQGQCPHCSAWNTLVEAVAAPAAASPRFQSWAANVTKVQKLSEVQTEETPRDPSGIDELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGRSRKVLYVSGEESAQQIALRASRLALDTSSVDLLAEICIENILETL
KREQPEVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPTANKPRQEIEGLKVLAVDRLDQAVEYCRE
>Mature_452_residues
AKNKTVFSCTECGGQSPKWQGQCPHCSAWNTLVEAVAAPAAASPRFQSWAANVTKVQKLSEVQTEETPRDPSGIDELDRV
LGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGRSRKVLYVSGEESAQQIALRASRLALDTSSVDLLAEICIENILETLK
REQPEVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGDS
HSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQPK
RLTVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEVR
PVTRGQERLKEAAKLGFTRAIVPTANKPRQEIEGLKVLAVDRLDQAVEYCRE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=454, Percent_Identity=58.5903083700441, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48804; Mature: 48673

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKTVFSCTECGGQSPKWQGQCPHCSAWNTLVEAVAAPAAASPRFQSWAANVTKVQKL
CCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SEVQTEETPRDPSGIDELDRVLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGRSRKVL
HHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
YVSGEESAQQIALRASRLALDTSSVDLLAEICIENILETLKREQPEVVVIDSIQTLYTEQ
EEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHH
VTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
HHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEEEECC
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEG
CCCHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCCCEEEEEHHCCCCCCCEEEEECCC
TRPLLVEIQALVDDCHGFQPKRLTVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGV
CCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCHHHEECCCE
KINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEVRPVTRGQERLKEAAKLGFTR
EECCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCE
AIVPTANKPRQEIEGLKVLAVDRLDQAVEYCRE
EECCCCCCCHHHHCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKNKTVFSCTECGGQSPKWQGQCPHCSAWNTLVEAVAAPAAASPRFQSWAANVTKVQKL
CCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SEVQTEETPRDPSGIDELDRVLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGRSRKVL
HHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
YVSGEESAQQIALRASRLALDTSSVDLLAEICIENILETLKREQPEVVVIDSIQTLYTEQ
EEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHH
VTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
HHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEEEECC
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEG
CCCHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCCCEEEEEHHCCCCCCCEEEEECCC
TRPLLVEIQALVDDCHGFQPKRLTVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGV
CCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCHHHEECCCE
KINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEVRPVTRGQERLKEAAKLGFTR
EECCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCE
AIVPTANKPRQEIEGLKVLAVDRLDQAVEYCRE
EECCCCCCCHHHHCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]