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The map label for this gene is proP [H]

Identifier: 34498356

GI number: 34498356

Start: 3177752

End: 3179065

Strand: Direct

Name: proP [H]

Synonym: CV_2901

Alternate gene names: 34498356

Gene position: 3177752-3179065 (Clockwise)

Preceding gene: 34498354

Following gene: 34498358

Centisome position: 66.88

GC content: 67.35

Gene sequence:

>1314_bases
ATGGGCACCCGCTCCCTAGAACCGCACCGTCCATTGAACCGCCAGGACTGCAAGACGCTGTTCCTCGCCGCGCTGGGCGG
CGCGCTGGAGTTCTACGACTTCATCATCTTCGTGTTTTTCGCCGTGGTGCTGGGCAAGCTGTTCTTCCCGCCCTCGATGC
CCGACTGGCTGAGCCAGCTGCAGACTTTCGGCATCTTCGCCGCCGGCTATCTGGCGCGGCCGCTGGGCGGCATCGTGATG
GCGCATTTCGGCGATCTGATGGGCCGCAAGCGCATGTTCACGCTCAGCATCGTGCTGATGGCGCTGCCGACGCTGGCCAT
GGGCATGCTGCCCACCTACCACGACATCGGCATCGCCGCCCCGCTGCTGCTGTTGGCGATGCGCATCCTGCAGGGCGCGG
CGATAGGCGGCGAGGTGCCCGGCGCCTGGGTGTTCGTCGCCGAGCACGCGCCCGCCCGCCACACCGGCTTCGCCTGCAGC
GTGATCACGTCCGGCCTGACCATAGGCATCCTGCTGGGCTCGCTGATCGCCACCGCGATCAACCTGGCCTTCAGCCCGCA
GCAGATCGCCGACTGGGCGTGGCGCGTGCCCTTCCTGGTGGGCGGCGTGTTCGGCCTGGTGGCGATGCAGCTGCGCCAAT
GGCTGCATGAGACGCCGGTGTTCGCGGAGATGCAGGCGCGCAAGGCCCTGTCCGAGGAGCTGCCGCTGAAAACGGTGCTG
AAGAAGCACAAGGCCGCCGTCGCGCTGTCGATGTTCGTCACCTGGTTCCTGGCCATCTCCATCGTCGTCGCCATCCTGAT
GGCGCCGGCTTATCTGCAAAAACAGTTCGGCCTGCCGGCGGTGGACACGCTGCGCGCCAACAGCCTGGCCATCGTCGCGC
TGACGCTGGGCTGCCTGGTGGCGGGCGTGTCCAGCGACCGTCTGGGCAGCGGCCCCACTTTCATCGCCGGCAGCCTGCTG
CTGGGCGCCAGCAGCTACGCCTTCTATTCGTCGATGGCCGCGCACGGCGGCCAGCTGTACACGCTTTACCCGCTGCTGGG
CTTCAGCGTCGGCATCGTCGGCGCGGTGCCGCTGGTGATGGTGCGCGCCTTCCCGGCCCAGGTGCGCTTTTCCGGCCTGT
CCTTCTCCTACAACGTCGCCTACGCCTTCGCCGGCGGCCTGACGCCCATCCTGCTGTCGATCTGGCTGAAATACGACGCG
CTGGCGCCGGCCTACTACCTGATGCTGCAATGCCTGATCGGCCTGCTGGTGGGCTGCTACCTGCTGCTCGCCGGAAAGAA
AACCGGCTCGGCTGCGCTGGCTCAGGAAGGCTGA

Upstream 100 bases:

>100_bases
TGTCAGACGCCGCCGCCGTCATTTAATCTGATTGGCACGACCTGACATTCCTCCACCATACGGTCGATAGATAACTACCA
AAGACAAAGGAAATCGCCGC

Downstream 100 bases:

>100_bases
ACCGGCATCACGCTCTCTTCGTCCACCGGCGGCAGCCTGGCCGCCGGGTCCTGGCGATAGAACTGGCTGAGCAGCCGGTA
CAGCTCGGGATAATCCCGCC

Product: MFS family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MGTRSLEPHRPLNRQDCKTLFLAALGGALEFYDFIIFVFFAVVLGKLFFPPSMPDWLSQLQTFGIFAAGYLARPLGGIVM
AHFGDLMGRKRMFTLSIVLMALPTLAMGMLPTYHDIGIAAPLLLLAMRILQGAAIGGEVPGAWVFVAEHAPARHTGFACS
VITSGLTIGILLGSLIATAINLAFSPQQIADWAWRVPFLVGGVFGLVAMQLRQWLHETPVFAEMQARKALSEELPLKTVL
KKHKAAVALSMFVTWFLAISIVVAILMAPAYLQKQFGLPAVDTLRANSLAIVALTLGCLVAGVSSDRLGSGPTFIAGSLL
LGASSYAFYSSMAAHGGQLYTLYPLLGFSVGIVGAVPLVMVRAFPAQVRFSGLSFSYNVAYAFAGGLTPILLSIWLKYDA
LAPAYYLMLQCLIGLLVGCYLLLAGKKTGSAALAQEG

Sequences:

>Translated_437_residues
MGTRSLEPHRPLNRQDCKTLFLAALGGALEFYDFIIFVFFAVVLGKLFFPPSMPDWLSQLQTFGIFAAGYLARPLGGIVM
AHFGDLMGRKRMFTLSIVLMALPTLAMGMLPTYHDIGIAAPLLLLAMRILQGAAIGGEVPGAWVFVAEHAPARHTGFACS
VITSGLTIGILLGSLIATAINLAFSPQQIADWAWRVPFLVGGVFGLVAMQLRQWLHETPVFAEMQARKALSEELPLKTVL
KKHKAAVALSMFVTWFLAISIVVAILMAPAYLQKQFGLPAVDTLRANSLAIVALTLGCLVAGVSSDRLGSGPTFIAGSLL
LGASSYAFYSSMAAHGGQLYTLYPLLGFSVGIVGAVPLVMVRAFPAQVRFSGLSFSYNVAYAFAGGLTPILLSIWLKYDA
LAPAYYLMLQCLIGLLVGCYLLLAGKKTGSAALAQEG
>Mature_436_residues
GTRSLEPHRPLNRQDCKTLFLAALGGALEFYDFIIFVFFAVVLGKLFFPPSMPDWLSQLQTFGIFAAGYLARPLGGIVMA
HFGDLMGRKRMFTLSIVLMALPTLAMGMLPTYHDIGIAAPLLLLAMRILQGAAIGGEVPGAWVFVAEHAPARHTGFACSV
ITSGLTIGILLGSLIATAINLAFSPQQIADWAWRVPFLVGGVFGLVAMQLRQWLHETPVFAEMQARKALSEELPLKTVLK
KHKAAVALSMFVTWFLAISIVVAILMAPAYLQKQFGLPAVDTLRANSLAIVALTLGCLVAGVSSDRLGSGPTFIAGSLLL
GASSYAFYSSMAAHGGQLYTLYPLLGFSVGIVGAVPLVMVRAFPAQVRFSGLSFSYNVAYAFAGGLTPILLSIWLKYDAL
APAYYLMLQCLIGLLVGCYLLLAGKKTGSAALAQEG

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=436, Percent_Identity=30.7339449541284, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788292, Length=413, Percent_Identity=30.2663438256659, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789941, Length=438, Percent_Identity=30.5936073059361, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788942, Length=444, Percent_Identity=29.5045045045045, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87082231, Length=449, Percent_Identity=24.0534521158129, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082404, Length=420, Percent_Identity=22.8571428571429, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081723, Length=208, Percent_Identity=29.3269230769231, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46672; Mature: 46541

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GTRSLEPHRPLNRQDCKTLFLAALGGALEFYDFIIFVFFAVVLGKLFFPPSMPDWLSQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH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HHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA