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The map label for this gene is kdsA

Identifier: 34498202

GI number: 34498202

Start: 2982400

End: 2983248

Strand: Direct

Name: kdsA

Synonym: CV_2747

Alternate gene names: 34498202

Gene position: 2982400-2983248 (Clockwise)

Preceding gene: 34498201

Following gene: 34498205

Centisome position: 62.77

GC content: 66.55

Gene sequence:

>849_bases
TTGATCAACGTCACTCTTTCCCCGTCCGTCACCGTCGGCAACGAAGCGCCGTTCACGCTGTTCGGCGGCATCAATGTGCT
GGAATCCCGCGACCTGGCGCTGCGCGCCGCCGAGGAATACGTCCGCGTCACCCAGAAGCTGGGCATCCCCTACGTGTTCA
AGGGCAGCTTCGACAAGGCCAACCGCTCGTCGATCCATTCTTACCGCGGCCCGGGCCTGGAAGAAGGCATGAAGATCCTG
CAGGCGGTGAAAGATACGTTCGGCGTGCCGGTGATCACCGACGTGCACGAGCCGTGGCAGGCCGCGCCGGTGGCCGAAGT
GGCCGACGTGGTGCAGCTGCCGGCCTTCCTCGCCCGTCAGACCGACCTGGTGGAAGCGCTGGCCAAGACCGGCCGCGCCG
TCAACATCAAGAAGCCGCAGTTCATGAGCCCGGCCCAGATTCAGAACGTGGTGGAAAAGTTCGTCGAAGCCGGCAACGAG
CAGCTGATCCTGTGCGACCGCGGCACCTGCCTCGGCTACGACAACCTGGTGGTGGACATGCTGGGCTTCGGCGTGATGAA
GAAAGTCAGCGGCAACCGCCCGGTGATCTTCGACGTCACCCACTCGCTGCAGCAGCGCGAATCCGGCGCCGCCGCTTCCG
GCGGCCGCCGCGCCCAGGTGGCGGAGCTGGCGCGCGCCGGCATGGCGGTGGGTTTGGCCGGCCTGTTCCTGGAGGCCCAC
CCGAATCCGGCCGAGGCCAAGTGCGACGGTCCCAGCGCGCTGCCGCTGGCGCAGCTGGAGCCCTTCCTGGCCCAAGTCAA
GGCCATCGACGATCTGGTCAAGTCCTTCGCCCCGCTGCAGATAGACTGA

Upstream 100 bases:

>100_bases
CGCCGGGATGGTCCGCGCCGCGCCGCGGGTATATCATGTCGCTTTGCTCAATCCGGCCGCGGCGTTCCGCCCGGCCGCGC
ACGCATACAGGAAGAATCCC

Downstream 100 bases:

>100_bases
GCCCGGCGGCCATGCGCCTCGCCGCGGCGGACCAGTCCGCCCGGCGCGGGTTCAAGCGCTCAGGCTGTTGAACAGCTCGT
CCTCCTGGGCGAAATGCAGT

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MINVTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKANRSSIHSYRGPGLEEGMKIL
QAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQTDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNE
QLILCDRGTCLGYDNLVVDMLGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAH
PNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVKSFAPLQID

Sequences:

>Translated_282_residues
MINVTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKANRSSIHSYRGPGLEEGMKIL
QAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQTDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNE
QLILCDRGTCLGYDNLVVDMLGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAH
PNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVKSFAPLQID
>Mature_282_residues
MINVTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKANRSSIHSYRGPGLEEGMKIL
QAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQTDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNE
QLILCDRGTCLGYDNLVVDMLGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAH
PNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVKSFAPLQID

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=270, Percent_Identity=77.4074074074074, Blast_Score=439, Evalue=1e-125,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_CHRVO (Q7NUF2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902417.1
- ProteinModelPortal:   Q7NUF2
- SMR:   Q7NUF2
- GeneID:   2547076
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2747
- NMPDR:   fig|243365.1.peg.2747
- HOGENOM:   HBG575990
- OMA:   QLILCDR
- PhylomeDB:   Q7NUF2
- ProtClustDB:   PRK05198
- BioCyc:   CVIO243365:CV_2747-MONOMER
- BRENDA:   2.5.1.55
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30220; Mature: 30220

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKA
CEEEEECCCEEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
NRSSIHSYRGPGLEEGMKILQAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQ
CHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNEQLILCDRGTCLGYDNLVVDM
HHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
LGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAH
HHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECC
PNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVKSFAPLQID
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MINVTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKA
CEEEEECCCEEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
NRSSIHSYRGPGLEEGMKILQAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQ
CHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNEQLILCDRGTCLGYDNLVVDM
HHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
LGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAH
HHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECC
PNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVKSFAPLQID
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782