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The map label for this gene is nfdA [H]

Identifier: 34498125

GI number: 34498125

Start: 2886315

End: 2888231

Strand: Direct

Name: nfdA [H]

Synonym: CV_2670

Alternate gene names: 34498125

Gene position: 2886315-2888231 (Clockwise)

Preceding gene: 34498123

Following gene: 34498126

Centisome position: 60.75

GC content: 64.42

Gene sequence:

>1917_bases
ATGAGCGATCATCACCACCACGACCATCATCTGGGCTGCGCTTGCTGCGCGCTGCCGTTGCTGGAACTGCTGGACCAGCC
GCAACTGTCGCAGGATGAATGGCAGCGAGAACTGGAAACGCTGGCGCCAGAACTGGGCAAGCGGTTCCCACGGCCCGGCC
AGCAGGAAAGCGCCATCTTTCACGGCGGCAGCATCCGACCGCTGGCGGCGGCCGGCCCCAGCCGCGTAGCCGCGCTGGGC
ATGTCCGGCGGGCGCATCGTGGCCATGGGGGACCTGGACGACGTGCGCGCGGCGATGGCCAGCCACCGTCCGTTCCGCGA
GATCGACCTGGACGGCCGCGCGCTGCTGCCGGGCCTGGTGGACCCTCACATGCACATCATGCCCAGCGCGCTGTTCAAAA
GCTGGATATCGCTGAGCGCCTTCGGCGTCACCCGAAACAGCCAGGACGGCGAAGACGCCCAGGTGCTGAATCCGGACTAC
AGCTACCAACAGATCAAGGACAAGCTCCTGCCGGCCATCGCGCAGGCAAAACAACCCGGCGCCGCATCCAACTGGGTGCT
CGGCTTCGGCGTCGACCCCTCGCTGATGCGGGAATGGGTGGATATCAAGGCCTCCTCCCTGGACGAGCTGGCTCCGCCGG
ACGACAACATCGCCGTCTTCCTGCTCAACGCCTCCGGCCACATCGGCTACGCCAACACCGCGGCGATGAAGGAAGCCGGA
CTCACGCCGGAACAGATCAAAAAGTGCGAAGGCGTGCTGACGGAAGCCAACATTATCCAGATCATGGGCAAGATTCCTAA
ACGGATACTGGCGAAAGACCTGCTGCGCAACCTGCTTATGGTGCTGCACGACGCGGCGTCCAGGGGCAACACCATGCTGT
TCGACGCGGGGCTGGGCTCGTCGCTCAAGGAAGCGGAAGTCCGGTTGCTCCAGCTGATCGCGAAACTCGGCCTGTCCCCG
GTGCGCATCGCCGGCGCCCTGTACTCCAACGAGCTGACGCAGATGCGGCAGTGGATGAAGCTCTACCAGCCTGACCTGAA
CAGCCAGGACGACAGCCACTTCAGCATCAAGGCGCTGAAACTGGTGGCCGACGGCTCCAACCAAGGCCTGACCGGCTTCC
AGTTCAGCCCCTACAAAAACGTCGCGCAACACCCGGTGCCGGACGTGCCGCCCATCGGCCTGTTCAACTTCAAAATCCCG
GATGCCCCCAGCTCGGAGAACGAGAATCGGCTGCCGGACTCTCTGGCAAGTTACCTCCATCTGGCGCTGGGCTCCGGCTG
GCCAGTGCTGGTGCACTCCAACGGCGAGCAGGCGATGGAGCTGGTGCTGGACAGCTACCAGGAGGCGATCAGCGCCACCC
GCGCCGACGCGCGCCACCTCCGCCTGCGGGTGGAGCACGCCTCGCTTCTCACCGACGCCACCTTGAGCCGCATGGCCAGC
ATGGGTCTGTCGCCCAGTTTCCTGATCGGCCACGTCGGCTATTGGGGTTATGCCCTTCAAAACGATATTCTGGGCGAAGC
GCGCACGGAGCTGCTGGACCGCTGTAAATCGGCGCGTGACGCCGGTATTCGCATCAGCCTTCATTCCGACCACTTCGTCT
CGCCCCTGGGCTCGCTGCGGATGGCGGAGCAGGCGATTTACCGCAAGATGGAAGGCGCGCCGCAAGAGGAAAAGCCCGTG
CTGAACAAAGAAGAATGTCTCAGCCATGAACAGGCGCTGGCCGCGGTCACGCTGGACGCGGCCTGGCAATGCCACATGGA
TCACCTGGTCGGCTCGCTGGAGCCGGGCAAGCTGGCCGACATGGTGATTCTGGGCCAGGACCCGCTCGACGATGGCGTGA
CCGACCTGCGCCACATCAACGTCCATGAAACCTGGCGCGGCGGCGCGAGGGTGTACGCCAATCCCAACACGCATTAA

Upstream 100 bases:

>100_bases
GGGCCACTCGCGGGACGGCGCAATCATGCCATGCGATTATCATCAAGCCGTACACGATGCCGGCAAACGGCGCCGGCCCA
AACCAAGGAGATGCACTGCC

Downstream 100 bases:

>100_bases
TCGCCAACACCCGGCCTGCGCCGGGACCATGCGCGGCCTGTCCTGCAATGGGCAGGCCGCTTGCCATATTTGAACATCAA
GCCGTCACCGCCCTAGAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSDHHHHDHHLGCACCALPLLELLDQPQLSQDEWQRELETLAPELGKRFPRPGQQESAIFHGGSIRPLAAAGPSRVAALG
MSGGRIVAMGDLDDVRAAMASHRPFREIDLDGRALLPGLVDPHMHIMPSALFKSWISLSAFGVTRNSQDGEDAQVLNPDY
SYQQIKDKLLPAIAQAKQPGAASNWVLGFGVDPSLMREWVDIKASSLDELAPPDDNIAVFLLNASGHIGYANTAAMKEAG
LTPEQIKKCEGVLTEANIIQIMGKIPKRILAKDLLRNLLMVLHDAASRGNTMLFDAGLGSSLKEAEVRLLQLIAKLGLSP
VRIAGALYSNELTQMRQWMKLYQPDLNSQDDSHFSIKALKLVADGSNQGLTGFQFSPYKNVAQHPVPDVPPIGLFNFKIP
DAPSSENENRLPDSLASYLHLALGSGWPVLVHSNGEQAMELVLDSYQEAISATRADARHLRLRVEHASLLTDATLSRMAS
MGLSPSFLIGHVGYWGYALQNDILGEARTELLDRCKSARDAGIRISLHSDHFVSPLGSLRMAEQAIYRKMEGAPQEEKPV
LNKEECLSHEQALAAVTLDAAWQCHMDHLVGSLEPGKLADMVILGQDPLDDGVTDLRHINVHETWRGGARVYANPNTH

Sequences:

>Translated_638_residues
MSDHHHHDHHLGCACCALPLLELLDQPQLSQDEWQRELETLAPELGKRFPRPGQQESAIFHGGSIRPLAAAGPSRVAALG
MSGGRIVAMGDLDDVRAAMASHRPFREIDLDGRALLPGLVDPHMHIMPSALFKSWISLSAFGVTRNSQDGEDAQVLNPDY
SYQQIKDKLLPAIAQAKQPGAASNWVLGFGVDPSLMREWVDIKASSLDELAPPDDNIAVFLLNASGHIGYANTAAMKEAG
LTPEQIKKCEGVLTEANIIQIMGKIPKRILAKDLLRNLLMVLHDAASRGNTMLFDAGLGSSLKEAEVRLLQLIAKLGLSP
VRIAGALYSNELTQMRQWMKLYQPDLNSQDDSHFSIKALKLVADGSNQGLTGFQFSPYKNVAQHPVPDVPPIGLFNFKIP
DAPSSENENRLPDSLASYLHLALGSGWPVLVHSNGEQAMELVLDSYQEAISATRADARHLRLRVEHASLLTDATLSRMAS
MGLSPSFLIGHVGYWGYALQNDILGEARTELLDRCKSARDAGIRISLHSDHFVSPLGSLRMAEQAIYRKMEGAPQEEKPV
LNKEECLSHEQALAAVTLDAAWQCHMDHLVGSLEPGKLADMVILGQDPLDDGVTDLRHINVHETWRGGARVYANPNTH
>Mature_637_residues
SDHHHHDHHLGCACCALPLLELLDQPQLSQDEWQRELETLAPELGKRFPRPGQQESAIFHGGSIRPLAAAGPSRVAALGM
SGGRIVAMGDLDDVRAAMASHRPFREIDLDGRALLPGLVDPHMHIMPSALFKSWISLSAFGVTRNSQDGEDAQVLNPDYS
YQQIKDKLLPAIAQAKQPGAASNWVLGFGVDPSLMREWVDIKASSLDELAPPDDNIAVFLLNASGHIGYANTAAMKEAGL
TPEQIKKCEGVLTEANIIQIMGKIPKRILAKDLLRNLLMVLHDAASRGNTMLFDAGLGSSLKEAEVRLLQLIAKLGLSPV
RIAGALYSNELTQMRQWMKLYQPDLNSQDDSHFSIKALKLVADGSNQGLTGFQFSPYKNVAQHPVPDVPPIGLFNFKIPD
APSSENENRLPDSLASYLHLALGSGWPVLVHSNGEQAMELVLDSYQEAISATRADARHLRLRVEHASLLTDATLSRMASM
GLSPSFLIGHVGYWGYALQNDILGEARTELLDRCKSARDAGIRISLHSDHFVSPLGSLRMAEQAIYRKMEGAPQEEKPVL
NKEECLSHEQALAAVTLDAAWQCHMDHLVGSLEPGKLADMVILGQDPLDDGVTDLRHINVHETWRGGARVYANPNTH

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 69768; Mature: 69637

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHHHHDHHLGCACCALPLLELLDQPQLSQDEWQRELETLAPELGKRFPRPGQQESAIF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
HGGSIRPLAAAGPSRVAALGMSGGRIVAMGDLDDVRAAMASHRPFREIDLDGRALLPGLV
ECCCCCCHHCCCCCCEEEEECCCCEEEEECCHHHHHHHHHCCCCCCEECCCCCEECCCCC
DPHMHIMPSALFKSWISLSAFGVTRNSQDGEDAQVLNPDYSYQQIKDKLLPAIAQAKQPG
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCC
AASNWVLGFGVDPSLMREWVDIKASSLDELAPPDDNIAVFLLNASGHIGYANTAAMKEAG
CCCCCEEEECCCHHHHHHHHHHHHHCHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHCC
LTPEQIKKCEGVLTEANIIQIMGKIPKRILAKDLLRNLLMVLHDAASRGNTMLFDAGLGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
SLKEAEVRLLQLIAKLGLSPVRIAGALYSNELTQMRQWMKLYQPDLNSQDDSHFSIKALK
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
LVADGSNQGLTGFQFSPYKNVAQHPVPDVPPIGLFNFKIPDAPSSENENRLPDSLASYLH
EEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
LALGSGWPVLVHSNGEQAMELVLDSYQEAISATRADARHLRLRVEHASLLTDATLSRMAS
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHH
MGLSPSFLIGHVGYWGYALQNDILGEARTELLDRCKSARDAGIRISLHSDHFVSPLGSLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCHHHHHH
MAEQAIYRKMEGAPQEEKPVLNKEECLSHEQALAAVTLDAAWQCHMDHLVGSLEPGKLAD
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
MVILGQDPLDDGVTDLRHINVHETWRGGARVYANPNTH
EEEECCCCCCCCHHHHEECCCHHCCCCCCEEECCCCCC
>Mature Secondary Structure 
SDHHHHDHHLGCACCALPLLELLDQPQLSQDEWQRELETLAPELGKRFPRPGQQESAIF
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
HGGSIRPLAAAGPSRVAALGMSGGRIVAMGDLDDVRAAMASHRPFREIDLDGRALLPGLV
ECCCCCCHHCCCCCCEEEEECCCCEEEEECCHHHHHHHHHCCCCCCEECCCCCEECCCCC
DPHMHIMPSALFKSWISLSAFGVTRNSQDGEDAQVLNPDYSYQQIKDKLLPAIAQAKQPG
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCC
AASNWVLGFGVDPSLMREWVDIKASSLDELAPPDDNIAVFLLNASGHIGYANTAAMKEAG
CCCCCEEEECCCHHHHHHHHHHHHHCHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHCC
LTPEQIKKCEGVLTEANIIQIMGKIPKRILAKDLLRNLLMVLHDAASRGNTMLFDAGLGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
SLKEAEVRLLQLIAKLGLSPVRIAGALYSNELTQMRQWMKLYQPDLNSQDDSHFSIKALK
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
LVADGSNQGLTGFQFSPYKNVAQHPVPDVPPIGLFNFKIPDAPSSENENRLPDSLASYLH
EEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
LALGSGWPVLVHSNGEQAMELVLDSYQEAISATRADARHLRLRVEHASLLTDATLSRMAS
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHH
MGLSPSFLIGHVGYWGYALQNDILGEARTELLDRCKSARDAGIRISLHSDHFVSPLGSLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCHHHHHH
MAEQAIYRKMEGAPQEEKPVLNKEECLSHEQALAAVTLDAAWQCHMDHLVGSLEPGKLAD
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
MVILGQDPLDDGVTDLRHINVHETWRGGARVYANPNTH
EEEECCCCCCCCHHHHEECCCHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA