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The map label for this gene is liaR [H]

Identifier: 34498054

GI number: 34498054

Start: 2807009

End: 2807662

Strand: Direct

Name: liaR [H]

Synonym: CV_2599

Alternate gene names: 34498054

Gene position: 2807009-2807662 (Clockwise)

Preceding gene: 34498045

Following gene: 34498056

Centisome position: 59.08

GC content: 55.66

Gene sequence:

>654_bases
ATGATGACGCCAGCCGCCCCTTCCGACGCTGCCGTACAACGGGTACTTTTGGTAGAAGACCATTCCCTATTGAGCAATGG
CATCAAAAACCTGTTATCGGCATTGCCAAGCTATCATGTGGTCGGTGAAGTCAATGATGGTCTGGGAGTCTATTCCGCCT
GTCAGCGGTTAATGCCGGATTTGGTATTGCTGGACTTGGGTTTGCCAGGCATGGATGGCATCGACGTCATTCGCCAATTG
AAACAACGCTGGCCGGAGCTGAACATCATCGTGATCACCGCCGACAGCGCCGAACATAGAGCGCGGGCGGCGCTGGATGC
GGGAGCGCTCGCCTATGTGCTGAAGAAAAGCCCGCAACAGACTTTGCTGGCCGCGCTACAGATTGCATCCCAGGGTCATA
TTTTCCTCGACCCGTCCCTCGATCTGGAACAAATCACCGAAACCCGCAACGTGGAAGGCCCAACCCGGCTGACCATGCGG
GAAAACCAGGTGCTGAAGCTAATCGCGGAAGGCATGCGCAACCGGGACATCGCGGAGAGTCTGAAAATCACCATCAAGAC
AGTGGAAACCCACCGGCTGAATCTGATGCGCAAACTGGATGCCCACAATGCGGTAGAGCTGACCAACTGGGCGATCCGAC
TAGGCATACACTAG

Upstream 100 bases:

>100_bases
TTGACTGCCTACTTACTTACGGTACTTGACGGAATCGGTAGAGGATCAATACTCTGATGAGGACATTCCTAAAATCAGCG
ATGCCAAATAGGTAGACATC

Downstream 100 bases:

>100_bases
CTCGGAGGTTTGCTTGCCCAAAACCGAAGAAAAGAAGAAGGTTAGTTAGGCCCAAGCACAGATCCATCGATGTGAGTCCG
GCCCGCATGAAATCATCCCC

Product: LuxR family two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MMTPAAPSDAAVQRVLLVEDHSLLSNGIKNLLSALPSYHVVGEVNDGLGVYSACQRLMPDLVLLDLGLPGMDGIDVIRQL
KQRWPELNIIVITADSAEHRARAALDAGALAYVLKKSPQQTLLAALQIASQGHIFLDPSLDLEQITETRNVEGPTRLTMR
ENQVLKLIAEGMRNRDIAESLKITIKTVETHRLNLMRKLDAHNAVELTNWAIRLGIH

Sequences:

>Translated_217_residues
MMTPAAPSDAAVQRVLLVEDHSLLSNGIKNLLSALPSYHVVGEVNDGLGVYSACQRLMPDLVLLDLGLPGMDGIDVIRQL
KQRWPELNIIVITADSAEHRARAALDAGALAYVLKKSPQQTLLAALQIASQGHIFLDPSLDLEQITETRNVEGPTRLTMR
ENQVLKLIAEGMRNRDIAESLKITIKTVETHRLNLMRKLDAHNAVELTNWAIRLGIH
>Mature_217_residues
MMTPAAPSDAAVQRVLLVEDHSLLSNGIKNLLSALPSYHVVGEVNDGLGVYSACQRLMPDLVLLDLGLPGMDGIDVIRQL
KQRWPELNIIVITADSAEHRARAALDAGALAYVLKKSPQQTLLAALQIASQGHIFLDPSLDLEQITETRNVEGPTRLTMR
ENQVLKLIAEGMRNRDIAESLKITIKTVETHRLNLMRKLDAHNAVELTNWAIRLGIH

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=200, Percent_Identity=34.5, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=31.4009661835749, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788712, Length=198, Percent_Identity=30.3030303030303, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=28.5, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=31.8407960199005, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787473, Length=204, Percent_Identity=35.7843137254902, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=28.8659793814433, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786911, Length=128, Percent_Identity=37.5, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23913; Mature: 23913

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTPAAPSDAAVQRVLLVEDHSLLSNGIKNLLSALPSYHVVGEVNDGLGVYSACQRLMPD
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCHH
LVLLDLGLPGMDGIDVIRQLKQRWPELNIIVITADSAEHRARAALDAGALAYVLKKSPQQ
HEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHH
TLLAALQIASQGHIFLDPSLDLEQITETRNVEGPTRLTMRENQVLKLIAEGMRNRDIAES
HHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHH
LKITIKTVETHRLNLMRKLDAHNAVELTNWAIRLGIH
HEEEEEEHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MMTPAAPSDAAVQRVLLVEDHSLLSNGIKNLLSALPSYHVVGEVNDGLGVYSACQRLMPD
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCHH
LVLLDLGLPGMDGIDVIRQLKQRWPELNIIVITADSAEHRARAALDAGALAYVLKKSPQQ
HEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHH
TLLAALQIASQGHIFLDPSLDLEQITETRNVEGPTRLTMRENQVLKLIAEGMRNRDIAES
HHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHH
LKITIKTVETHRLNLMRKLDAHNAVELTNWAIRLGIH
HEEEEEEHHHHHHHHHHHHCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]