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The map label for this gene is dusC [H]

Identifier: 34497784

GI number: 34497784

Start: 2519757

End: 2520704

Strand: Direct

Name: dusC [H]

Synonym: CV_2329

Alternate gene names: 34497784

Gene position: 2519757-2520704 (Clockwise)

Preceding gene: 34497773

Following gene: 34497785

Centisome position: 53.04

GC content: 64.77

Gene sequence:

>948_bases
ATGAAGCTGGTGCTTGCCCCGATGGAGGGCCTGGTTGACGATGTGATGCGCGATGTGCTGACTCGCGTGGGCGGCATCGA
TCTTTGCGTGACCGAGTTCGTGCGCGTCACCTCCGTGCTGCTGCCCACCCGCACCTTCATGCGTTTGGCGCCGGAGCTCG
ACCATCAGGGCAAGACCCGGGCCGGCGTGCCGGTGCGGGTGCAGTTGCTGGGCTCCGATGTTTCCTGTCTGGCCGAGAAT
GGCGCCAAGGCGGCTAGCTTGGGCGCGCCGGGCGTGGACCTGAATTTCGGCTGTCCGGCGCCCACTGTCAACCGCCACCG
CGGCGGCGCGGTTTTGCTGAACGAGCCGGAACTGCTGAACCAGATCGTCGCCGCGGTTCGCCGCGCGGTGCCGCCGGAAG
TGCCGGTGACGGCCAAGATGCGCTTGGGCTTCGAGGATAAAAGCCGGGCGCTGGAGTGCGCGCAGGCGATAGCCAGCGCC
GGCGCCGACGAATTGACCGTCCACGGCCGCACCAAGGTGGAGGGCTACAAGCCGCCGGCGCATTGGGACTGGATCGCCCG
GATCCGGGAAAGCGTGACGATTCCGGTCACGGCCAACGGCGAGGTATGGACGCTGGCCGATTACCGCGCGATCCGGGAGG
AGAGCGGCTGCGACACAGTGATGATAGGGCGCGGCCTGGTGGCCTGCCCGGATCTGGCGCTCAGGATTGCGGAAGGTGGG
CCGGCCGAACCCATGTCCTGGGGCGAAATGATGCCTTGGGTGCTGGACTTCTTCCGTCTGTGCCGGCAAAAGTCAGGAGA
AGAGCGTTATCCGGCAGCCAGGCTCAAGCAATGGCTGGGTCTGCTCAAGCGCACCTGGCCAGAGGCCGAGCGTTTGTTTG
AAACGGTAAGGCGCGAGCAAGAGGCTGAAAAGATTGAGAATATTCTGATTGCAAGACGGGATAAGTAA

Upstream 100 bases:

>100_bases
CTCCGGGGTGGCGATGCTCAACTGTATGTCCATCTACAATCTCCGTGATGGGAAGGGCGGCGCTGGGGTACAATAACGCC
CTTTTTCAGCAGGTTAGACA

Downstream 100 bases:

>100_bases
ATATACTCATACCGCAATGCGGAAACTGTTGCTAACATCAAAACGCGTGCTGCTTTGGTAAAGCAGCGCGCTGTAACATC
TGCCGGCCTGCCTGGCTGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKLVLAPMEGLVDDVMRDVLTRVGGIDLCVTEFVRVTSVLLPTRTFMRLAPELDHQGKTRAGVPVRVQLLGSDVSCLAEN
GAKAASLGAPGVDLNFGCPAPTVNRHRGGAVLLNEPELLNQIVAAVRRAVPPEVPVTAKMRLGFEDKSRALECAQAIASA
GADELTVHGRTKVEGYKPPAHWDWIARIRESVTIPVTANGEVWTLADYRAIREESGCDTVMIGRGLVACPDLALRIAEGG
PAEPMSWGEMMPWVLDFFRLCRQKSGEERYPAARLKQWLGLLKRTWPEAERLFETVRREQEAEKIENILIARRDK

Sequences:

>Translated_315_residues
MKLVLAPMEGLVDDVMRDVLTRVGGIDLCVTEFVRVTSVLLPTRTFMRLAPELDHQGKTRAGVPVRVQLLGSDVSCLAEN
GAKAASLGAPGVDLNFGCPAPTVNRHRGGAVLLNEPELLNQIVAAVRRAVPPEVPVTAKMRLGFEDKSRALECAQAIASA
GADELTVHGRTKVEGYKPPAHWDWIARIRESVTIPVTANGEVWTLADYRAIREESGCDTVMIGRGLVACPDLALRIAEGG
PAEPMSWGEMMPWVLDFFRLCRQKSGEERYPAARLKQWLGLLKRTWPEAERLFETVRREQEAEKIENILIARRDK
>Mature_315_residues
MKLVLAPMEGLVDDVMRDVLTRVGGIDLCVTEFVRVTSVLLPTRTFMRLAPELDHQGKTRAGVPVRVQLLGSDVSCLAEN
GAKAASLGAPGVDLNFGCPAPTVNRHRGGAVLLNEPELLNQIVAAVRRAVPPEVPVTAKMRLGFEDKSRALECAQAIASA
GADELTVHGRTKVEGYKPPAHWDWIARIRESVTIPVTANGEVWTLADYRAIREESGCDTVMIGRGLVACPDLALRIAEGG
PAEPMSWGEMMPWVLDFFRLCRQKSGEERYPAARLKQWLGLLKRTWPEAERLFETVRREQEAEKIENILIARRDK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=229, Percent_Identity=33.1877729257642, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=31.3807531380753, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=27.4900398406374, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI239788483, Length=293, Percent_Identity=25.938566552901, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI239788462, Length=293, Percent_Identity=25.938566552901, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788462, Length=300, Percent_Identity=47, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1789660, Length=227, Percent_Identity=33.920704845815, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693211, Length=273, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=29.9065420560748, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=232, Percent_Identity=30.1724137931034, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=247, Percent_Identity=26.7206477732794, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17510279, Length=249, Percent_Identity=27.3092369477912, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=171, Percent_Identity=32.1637426900585, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=186, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921524, Length=159, Percent_Identity=36.4779874213836, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24580595, Length=238, Percent_Identity=31.0924369747899, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920448, Length=238, Percent_Identity=31.0924369747899, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585320, Length=297, Percent_Identity=27.6094276094276, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45549423, Length=138, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34765; Mature: 34765

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVLAPMEGLVDDVMRDVLTRVGGIDLCVTEFVRVTSVLLPTRTFMRLAPELDHQGKTR
CCEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AGVPVRVQLLGSDVSCLAENGAKAASLGAPGVDLNFGCPAPTVNRHRGGAVLLNEPELLN
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHH
QIVAAVRRAVPPEVPVTAKMRLGFEDKSRALECAQAIASAGADELTVHGRTKVEGYKPPA
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCC
HWDWIARIRESVTIPVTANGEVWTLADYRAIREESGCDTVMIGRGLVACPDLALRIAEGG
HHHHHHHHHCCEEEEEECCCCEEEHHHHHHHHHCCCCCEEEECCCCCCCCHHHEEECCCC
PAEPMSWGEMMPWVLDFFRLCRQKSGEERYPAARLKQWLGLLKRTWPEAERLFETVRREQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EAEKIENILIARRDK
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLVLAPMEGLVDDVMRDVLTRVGGIDLCVTEFVRVTSVLLPTRTFMRLAPELDHQGKTR
CCEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AGVPVRVQLLGSDVSCLAENGAKAASLGAPGVDLNFGCPAPTVNRHRGGAVLLNEPELLN
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHH
QIVAAVRRAVPPEVPVTAKMRLGFEDKSRALECAQAIASAGADELTVHGRTKVEGYKPPA
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCC
HWDWIARIRESVTIPVTANGEVWTLADYRAIREESGCDTVMIGRGLVACPDLALRIAEGG
HHHHHHHHHCCEEEEEECCCCEEEHHHHHHHHHCCCCCEEEECCCCCCCCHHHEEECCCC
PAEPMSWGEMMPWVLDFFRLCRQKSGEERYPAARLKQWLGLLKRTWPEAERLFETVRREQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EAEKIENILIARRDK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA