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The map label for this gene is nrdB [H]

Identifier: 34497739

GI number: 34497739

Start: 2469359

End: 2470441

Strand: Reverse

Name: nrdB [H]

Synonym: CV_2284

Alternate gene names: 34497739

Gene position: 2470441-2469359 (Counterclockwise)

Preceding gene: 34497740

Following gene: 34497738

Centisome position: 52.0

GC content: 58.73

Gene sequence:

>1083_bases
ATGCTGAATTTCGAAGACGCCGTCCCCGCCCCGTCCGCCGCCCCCTCCTCCATGGACGCCCATGGCACCGGCCGCGTAAA
CGTGGTGGACAAGAAGGTGATCAACGGCACGACCGACGTCAACCAGCTGGTACCGTTCAAGCACAAGTGGGCTTGGGAAA
AGTACCTGGCGCAGTGCGCCAACCACTGGATGCCGCAGGAAGTGAACATGCAGCGCGACATCGAACAATGGAAAACCGGC
CAGCTGACCGAAGACGAAATGCGCATCGTCAAGCGCAACCTGGGCTTCTTCGTCACCGCGGATTCCCTGGCCGCAAACAA
CATCGTGCTGGGCACCTATCGCCAGATCACCAGCCCGGAATGCCGCCAGTTCCTGCTGCGCCAGGCCTTTGACGAAGCCA
TCCACACCCACGCCTACCAATACATCGTGGAAAGCCTGGGCCTGGACGAAGGCGAAGTGTTCAACGCCTATCACGAAGTG
AAGTCCATCCGCGACAAGGACGAATTCCTGATCCCGTTCATCGACGTGCTGTGCAACCCGGAATTCAAGACCGGCACCGT
CGAAAACGACCAGAAGCTGCTCAAATCCCTGATCGTGTTCGCCTGCATCATGGAAGGCCTGTTCTTCTACGTCGGCTTCG
TGCAAATCCTGGCCCTGGGCCGCCAGAACAAGATGACCGGCGCCGCCGAACAGTACCAGTACATCCTGCGCGACGAGTCC
ATGCACTGCAACTTCGGCATCGACCTGATCAACACCATCAAGCTGGAAAACCCGCTGCTGTGGACCGACAGCTTCAAGGC
CGAGATCGTGGACCTGTTCAAGCAAGCCGTCGAGCTGGAATACGCCTACGCCGAAGACACCATGCCGCGCGGCGTGCTGG
GCCTGAACGCCAGCATGTTCAAGGAATACCTGCGCTTCATCGCCAACCGCCGCATGCAGCAAATTGGCCTGGACCCGCTG
TTCCCGGGTGTCAACAACCCCTTCCCGTGGATGAGCGAGATGATCGACTTGAAGAAGGAAAAGAACTTCTTTGAGACGCG
GGTTACTGAATATCAGACTGGCGGGGCGTTGAGCTGGGATTGA

Upstream 100 bases:

>100_bases
CCGGCCCAGGCCATGCAGACGCCGCAAGCAGAACCAGCCGTAAAAACCGCATAAGCAAACCGCCGCGCCCGCGCGGCAAA
ACCAAGAATCAGGAGCACCC

Downstream 100 bases:

>100_bases
TTTGACATTAAGATTTGACAGCTCCTTATGCAAAAGGCACACTCTTAGTGTGCCTTTTTCTTTAAGGAAAAAAGATGTCT
ACCAATAATACTGTGAACCA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLNFEDAVPAPSAAPSSMDAHGTGRVNVVDKKVINGTTDVNQLVPFKHKWAWEKYLAQCANHWMPQEVNMQRDIEQWKTG
QLTEDEMRIVKRNLGFFVTADSLAANNIVLGTYRQITSPECRQFLLRQAFDEAIHTHAYQYIVESLGLDEGEVFNAYHEV
KSIRDKDEFLIPFIDVLCNPEFKTGTVENDQKLLKSLIVFACIMEGLFFYVGFVQILALGRQNKMTGAAEQYQYILRDES
MHCNFGIDLINTIKLENPLLWTDSFKAEIVDLFKQAVELEYAYAEDTMPRGVLGLNASMFKEYLRFIANRRMQQIGLDPL
FPGVNNPFPWMSEMIDLKKEKNFFETRVTEYQTGGALSWD

Sequences:

>Translated_360_residues
MLNFEDAVPAPSAAPSSMDAHGTGRVNVVDKKVINGTTDVNQLVPFKHKWAWEKYLAQCANHWMPQEVNMQRDIEQWKTG
QLTEDEMRIVKRNLGFFVTADSLAANNIVLGTYRQITSPECRQFLLRQAFDEAIHTHAYQYIVESLGLDEGEVFNAYHEV
KSIRDKDEFLIPFIDVLCNPEFKTGTVENDQKLLKSLIVFACIMEGLFFYVGFVQILALGRQNKMTGAAEQYQYILRDES
MHCNFGIDLINTIKLENPLLWTDSFKAEIVDLFKQAVELEYAYAEDTMPRGVLGLNASMFKEYLRFIANRRMQQIGLDPL
FPGVNNPFPWMSEMIDLKKEKNFFETRVTEYQTGGALSWD
>Mature_360_residues
MLNFEDAVPAPSAAPSSMDAHGTGRVNVVDKKVINGTTDVNQLVPFKHKWAWEKYLAQCANHWMPQEVNMQRDIEQWKTG
QLTEDEMRIVKRNLGFFVTADSLAANNIVLGTYRQITSPECRQFLLRQAFDEAIHTHAYQYIVESLGLDEGEVFNAYHEV
KSIRDKDEFLIPFIDVLCNPEFKTGTVENDQKLLKSLIVFACIMEGLFFYVGFVQILALGRQNKMTGAAEQYQYILRDES
MHCNFGIDLINTIKLENPLLWTDSFKAEIVDLFKQAVELEYAYAEDTMPRGVLGLNASMFKEYLRFIANRRMQQIGLDPL
FPGVNNPFPWMSEMIDLKKEKNFFETRVTEYQTGGALSWD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI289177074, Length=351, Percent_Identity=24.5014245014245, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI260064013, Length=319, Percent_Identity=26.6457680250784, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557845, Length=319, Percent_Identity=26.6457680250784, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI42544136, Length=356, Percent_Identity=24.438202247191, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI289177076, Length=295, Percent_Identity=25.7627118644068, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788567, Length=296, Percent_Identity=26.6891891891892, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17551968, Length=351, Percent_Identity=27.6353276353276, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI193206379, Length=303, Percent_Identity=22.7722772277228, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567153, Length=227, Percent_Identity=25.5506607929515, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322434, Length=330, Percent_Identity=29.0909090909091, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321619, Length=324, Percent_Identity=23.4567901234568, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18079279, Length=319, Percent_Identity=25.705329153605, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 41406; Mature: 41406

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFEDAVPAPSAAPSSMDAHGTGRVNVVDKKVINGTTDVNQLVPFKHKWAWEKYLAQCA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHH
NHWMPQEVNMQRDIEQWKTGQLTEDEMRIVKRNLGFFVTADSLAANNIVLGTYRQITSPE
HCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEECHHHCCCHH
CRQFLLRQAFDEAIHTHAYQYIVESLGLDEGEVFNAYHEVKSIRDKDEFLIPFIDVLCNP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EFKTGTVENDQKLLKSLIVFACIMEGLFFYVGFVQILALGRQNKMTGAAEQYQYILRDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
MHCNFGIDLINTIKLENPLLWTDSFKAEIVDLFKQAVELEYAYAEDTMPRGVLGLNASMF
CCCCCCCHHHHHEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
KEYLRFIANRRMQQIGLDPLFPGVNNPFPWMSEMIDLKKEKNFFETRVTEYQTGGALSWD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLNFEDAVPAPSAAPSSMDAHGTGRVNVVDKKVINGTTDVNQLVPFKHKWAWEKYLAQCA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHH
NHWMPQEVNMQRDIEQWKTGQLTEDEMRIVKRNLGFFVTADSLAANNIVLGTYRQITSPE
HCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEECHHHCCCHH
CRQFLLRQAFDEAIHTHAYQYIVESLGLDEGEVFNAYHEVKSIRDKDEFLIPFIDVLCNP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EFKTGTVENDQKLLKSLIVFACIMEGLFFYVGFVQILALGRQNKMTGAAEQYQYILRDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
MHCNFGIDLINTIKLENPLLWTDSFKAEIVDLFKQAVELEYAYAEDTMPRGVLGLNASMF
CCCCCCCHHHHHEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
KEYLRFIANRRMQQIGLDPLFPGVNNPFPWMSEMIDLKKEKNFFETRVTEYQTGGALSWD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]