Click here to switch to the map view.

The map label for this gene is ibeB [H]

Identifier: 34497697

GI number: 34497697

Start: 2430434

End: 2431813

Strand: Direct

Name: ibeB [H]

Synonym: CV_2242

Alternate gene names: 34497697

Gene position: 2430434-2431813 (Clockwise)

Preceding gene: 34497696

Following gene: 34497701

Centisome position: 51.16

GC content: 73.62

Gene sequence:

>1380_bases
ATGAAGCGGGCTTTCTGCTTATTGATGGGCGCGGCTTTGGCTGGCTGCGCGTCGCCGCAGCCGGATTACCAACGGCCCGC
CGCGCCGGTGCCGGCGCATTGGCCGGCGGCGGACGCGGAAAGCGGTCAGGCCGATATCGCCTGGCGGCGCTTCTTCGTCG
ACGACGGCCTGCGCGAAACCATCGCCCAGGCATTGGCCAACAACCGCGATTTGCGCGTAGCCGCCGCCAATGTCCAGGCG
GCGCGGGCGCAGTACCGGATACTGGACGCGGCGCGGCTGCCGGCCGTGCAGCTGCAGGGCAAGTCCGACCGCCAGCTGAC
CCGCGGCGGCGATCCGTCGCTGGCGGCGGACAGCCGCGGCGTCAGCGCCGGGCTGGGCGTCAGCGCGTTCGAGCTGGATC
TGTTCGGCCGGGTGCGGAGCCTGAGCGACGCGCAATGGCGGCGCTATCTGGCCAGCGAGGCCGGCGCGCAGGCGGCGCGG
GTCAGCCTGATCGCGGAAACCGCCAACGCCTACCTGACATTGGCCGCCGACCGCAGCCAGCTGGCCGCGGCGCGGACGAC
GCTGCGAAGCGCCGAGCGCTCGCTGGAACTGACGCGCGAGCGGCTGCGGCTGGGGGCGTCTTCCGCGCTGGATGTCAGCG
CCATGGAAACGGTGCGGCAGCAGGCGCGGGCCGATGTCGCCCGCTACCAAGGCCAGGCGGAGCGGGACCGCCTGGCGCTG
CAATTGCTGGTCGGCGCGGAACTGGACCAGACGCGGGTGCCGGCGGCGCTGGAGGACAGGCCGTACACGCTGGCGGCGCT
GCCGGCGGACGCGCCGGCCGATGCCTTGCTGCGCCGGCCGGATGTCCGCCAGGCGGAAAATCTGCTGCGGGCCGCCCATG
CCGACGTGGACGCCGCGCGGGCGGGCCTGTGGCCCAAGCTGGGGCTCAGCGCCAGCTCAGGGCTGAGCGGCGTGACGCTG
GCCGGGCTGCTGAACGCGCCGGCGCGGGCCTTGAGCCTGGCGCTGGACGCCAGCTTGCCGGCGCTTGACGGCGGCGCGGC
GAAGGCTGGAGTCGATCAGGCAGAGGCGCAGCGCCTGGCGGCGCTGGCCGCCTACGAGAAAGCTTTGCAGAACGGCTTCC
GTGAAGTGGCTGGCGCCTTGAGCCAGCGCCAGGCGCTGGCGGAGGAGAAACAGGCGCGGCAGGCCGCGCTCGCCAGCGCG
GAGGGCTCGCTGCGGCTGGCGGAGGCGCGCTATCGGCAGGGGCTGGACGGCTATCTGAGCCTGCTGGAGGCGCAGCGGAC
GGCGCAGGCGGCGCGCCTGCAGTGGATCGGCGCCCACCTGGCCGAAGCGGAGAATATGGCGGCGCTGTACCGGTCTCTGG
GCGGCGGGCTGGAGAGCTGA

Upstream 100 bases:

>100_bases
CGTTGTTGACTCTGTTCTTCGTGCCGCTGGCGCATGTGTTGATCCGGGGCCTGGCCGGCCGCGTCGCAGAGCGGCGCTGG
AAGCCGGCGGAGGCGCGGCC

Downstream 100 bases:

>100_bases
TCCCGCCGCGGCCTCAGCTCTGGATGTCCAGCCGCGGCAACTGGCCGCCGGCCATCCGGTTGTCGGGCGCGTAGGGAAAA
ATCAGTTCTATCCTGACGCC

Product: outer membrane drug efflux lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKRAFCLLMGAALAGCASPQPDYQRPAAPVPAHWPAADAESGQADIAWRRFFVDDGLRETIAQALANNRDLRVAAANVQA
ARAQYRILDAARLPAVQLQGKSDRQLTRGGDPSLAADSRGVSAGLGVSAFELDLFGRVRSLSDAQWRRYLASEAGAQAAR
VSLIAETANAYLTLAADRSQLAAARTTLRSAERSLELTRERLRLGASSALDVSAMETVRQQARADVARYQGQAERDRLAL
QLLVGAELDQTRVPAALEDRPYTLAALPADAPADALLRRPDVRQAENLLRAAHADVDAARAGLWPKLGLSASSGLSGVTL
AGLLNAPARALSLALDASLPALDGGAAKAGVDQAEAQRLAALAAYEKALQNGFREVAGALSQRQALAEEKQARQAALASA
EGSLRLAEARYRQGLDGYLSLLEAQRTAQAARLQWIGAHLAEAENMAALYRSLGGGLES

Sequences:

>Translated_459_residues
MKRAFCLLMGAALAGCASPQPDYQRPAAPVPAHWPAADAESGQADIAWRRFFVDDGLRETIAQALANNRDLRVAAANVQA
ARAQYRILDAARLPAVQLQGKSDRQLTRGGDPSLAADSRGVSAGLGVSAFELDLFGRVRSLSDAQWRRYLASEAGAQAAR
VSLIAETANAYLTLAADRSQLAAARTTLRSAERSLELTRERLRLGASSALDVSAMETVRQQARADVARYQGQAERDRLAL
QLLVGAELDQTRVPAALEDRPYTLAALPADAPADALLRRPDVRQAENLLRAAHADVDAARAGLWPKLGLSASSGLSGVTL
AGLLNAPARALSLALDASLPALDGGAAKAGVDQAEAQRLAALAAYEKALQNGFREVAGALSQRQALAEEKQARQAALASA
EGSLRLAEARYRQGLDGYLSLLEAQRTAQAARLQWIGAHLAEAENMAALYRSLGGGLES
>Mature_459_residues
MKRAFCLLMGAALAGCASPQPDYQRPAAPVPAHWPAADAESGQADIAWRRFFVDDGLRETIAQALANNRDLRVAAANVQA
ARAQYRILDAARLPAVQLQGKSDRQLTRGGDPSLAADSRGVSAGLGVSAFELDLFGRVRSLSDAQWRRYLASEAGAQAAR
VSLIAETANAYLTLAADRSQLAAARTTLRSAERSLELTRERLRLGASSALDVSAMETVRQQARADVARYQGQAERDRLAL
QLLVGAELDQTRVPAALEDRPYTLAALPADAPADALLRRPDVRQAENLLRAAHADVDAARAGLWPKLGLSASSGLSGVTL
AGLLNAPARALSLALDASLPALDGGAAKAGVDQAEAQRLAALAAYEKALQNGFREVAGALSQRQALAEEKQARQAALASA
EGSLRLAEARYRQGLDGYLSLLEAQRTAQAARLQWIGAHLAEAENMAALYRSLGGGLES

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=459, Percent_Identity=35.7298474945534, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI2367348, Length=479, Percent_Identity=24.4258872651357, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 48519; Mature: 48519

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAFCLLMGAALAGCASPQPDYQRPAAPVPAHWPAADAESGQADIAWRRFFVDDGLRET
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHH
IAQALANNRDLRVAAANVQAARAQYRILDAARLPAVQLQGKSDRQLTRGGDPSLAADSRG
HHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCC
VSAGLGVSAFELDLFGRVRSLSDAQWRRYLASEAGAQAARVSLIAETANAYLTLAADRSQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHH
LAAARTTLRSAERSLELTRERLRLGASSALDVSAMETVRQQARADVARYQGQAERDRLAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QLLVGAELDQTRVPAALEDRPYTLAALPADAPADALLRRPDVRQAENLLRAAHADVDAAR
HHHHCCCCCHHCCCCCCCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHCCHHHHH
AGLWPKLGLSASSGLSGVTLAGLLNAPARALSLALDASLPALDGGAAKAGVDQAEAQRLA
HCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
ALAAYEKALQNGFREVAGALSQRQALAEEKQARQAALASAEGSLRLAEARYRQGLDGYLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
LLEAQRTAQAARLQWIGAHLAEAENMAALYRSLGGGLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRAFCLLMGAALAGCASPQPDYQRPAAPVPAHWPAADAESGQADIAWRRFFVDDGLRET
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHH
IAQALANNRDLRVAAANVQAARAQYRILDAARLPAVQLQGKSDRQLTRGGDPSLAADSRG
HHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCC
VSAGLGVSAFELDLFGRVRSLSDAQWRRYLASEAGAQAARVSLIAETANAYLTLAADRSQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHH
LAAARTTLRSAERSLELTRERLRLGASSALDVSAMETVRQQARADVARYQGQAERDRLAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QLLVGAELDQTRVPAALEDRPYTLAALPADAPADALLRRPDVRQAENLLRAAHADVDAAR
HHHHCCCCCHHCCCCCCCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHCCHHHHH
AGLWPKLGLSASSGLSGVTLAGLLNAPARALSLALDASLPALDGGAAKAGVDQAEAQRLA
HCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
ALAAYEKALQNGFREVAGALSQRQALAEEKQARQAALASAEGSLRLAEARYRQGLDGYLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
LLEAQRTAQAARLQWIGAHLAEAENMAALYRSLGGGLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]