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The map label for this gene is mexA [H]

Identifier: 34497695

GI number: 34497695

Start: 2426145

End: 2427305

Strand: Direct

Name: mexA [H]

Synonym: CV_2240

Alternate gene names: 34497695

Gene position: 2426145-2427305 (Clockwise)

Preceding gene: 34497692

Following gene: 34497696

Centisome position: 51.07

GC content: 71.23

Gene sequence:

>1161_bases
ATGGTTTCCGTTTTCGCGGCTGGAATGCACTGGAATATGCGGATATTGACGGTTGTTGCGATGGCGGCGAGCCTGGCCGG
CTGCGGGGCGGCGGAGCGGACGCCGGAGGCGGGCAAGCCCCCCGAGGTGGGCGTGGTGAGGCTGGAAGCGAAGGATGTGG
CGATACGGGCCGAGCTGGCCGGCCGCACCGTCGCCTACCGCAGCGCCGAAGTCAGGCCGCAGGTGAACGGCATCGTGCTG
AAACGGCATTTCACCGAGGGCAGCCTGGTCAAGGCCGGCGACCTGCTGTACCAGATCGACCCGGCCGGCTATCAGGCGGC
CTTGCGGCAGGCGGAGGCGACGCTGGCCTCCGCCCGCGCCGCGCTGGGCAGCCTGAAGGGCCGGGCCGAGCGCTATGCCG
AGCTGGCCAAGATAGACGGCGTCAGCCGTCAGGAGCGGGAGGAGGCGGATGCCGACTATCGCAAGGGTTTGGCTGCGGCG
AAAGCCGGCGAGGCGGCGGTGGCGGCGGCCAGGGTGGATGTGTCGCGCACCCGCGTGCTGGCGCCGATTTCCGGCCGCAT
CGGCCGCTCCGCCGTCAGCGAGGGCGCTTTGGTCACTTCGGGCCAGAGCGCGGCGTTGGCCACCATCCAGCAGCTGGACC
CGATGTACGTGGACATCGTCCAGTCCAGCCAGGATCTGCTGGCGTTCAAGCGCCGGCAGGCCGACGGCGAGCTTGAGCCG
GCCGGCGCGGCCGCCAGGCTGAAACTGGAGGACGGCGCGCCTTACCGGCATCCCGGCAAGCTGCGCTTCACCGAATTGAA
CGTCGACCGCGTGTCCGGCGCGGTGACCTTGCGCGCCGAATTCCCCAACCCCGAAGGCCTGCTGCTGCCCGGCATGTATG
TGCGCGCCGAGCTGGAGCAGGGCGTGCGCCGCCAGGCGGTATTGGCGCCGCAGCAAGGCGTGGCGCGCGACGACAAGGGC
CGCGCCACCGCCCTGGTGCTGGGCGCCGGCGACAAGGTGGAGCAGCGGCTGTTGACGACCGAGGGCACCCACGGCGCCTA
CTGGCTGGTGACCGCAGGACTGAAAGCCGGCGACCGCCTGATCGTCGACGGCCTGCAGCGCGCCCGCCCGGGCGAGGCGG
CCAAGCCGGTGGCCTTGCCGGCCTATGCCGGAAGGAGCTGA

Upstream 100 bases:

>100_bases
CGACGCCGTTTGGACACTGGACTCGATTGAGCCCGGAGCGAGAGCCGTCAAGCCCCGTTGTCGATGACAGCATGCGTCCG
CGCGCACATCGGATGGGGGG

Downstream 100 bases:

>100_bases
GCGATGCTGGCGCGTTTCTTCATAGACCGCCCGGTCTTCGCCTGGGTGCTGGCCATCGTCATCATGCTGGCCGGCCTGTT
CGCGCTGCGCGCGCTGCCGG

Product: multidrug ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MVSVFAAGMHWNMRILTVVAMAASLAGCGAAERTPEAGKPPEVGVVRLEAKDVAIRAELAGRTVAYRSAEVRPQVNGIVL
KRHFTEGSLVKAGDLLYQIDPAGYQAALRQAEATLASARAALGSLKGRAERYAELAKIDGVSRQEREEADADYRKGLAAA
KAGEAAVAAARVDVSRTRVLAPISGRIGRSAVSEGALVTSGQSAALATIQQLDPMYVDIVQSSQDLLAFKRRQADGELEP
AGAAARLKLEDGAPYRHPGKLRFTELNVDRVSGAVTLRAEFPNPEGLLLPGMYVRAELEQGVRRQAVLAPQQGVARDDKG
RATALVLGAGDKVEQRLLTTEGTHGAYWLVTAGLKAGDRLIVDGLQRARPGEAAKPVALPAYAGRS

Sequences:

>Translated_386_residues
MVSVFAAGMHWNMRILTVVAMAASLAGCGAAERTPEAGKPPEVGVVRLEAKDVAIRAELAGRTVAYRSAEVRPQVNGIVL
KRHFTEGSLVKAGDLLYQIDPAGYQAALRQAEATLASARAALGSLKGRAERYAELAKIDGVSRQEREEADADYRKGLAAA
KAGEAAVAAARVDVSRTRVLAPISGRIGRSAVSEGALVTSGQSAALATIQQLDPMYVDIVQSSQDLLAFKRRQADGELEP
AGAAARLKLEDGAPYRHPGKLRFTELNVDRVSGAVTLRAEFPNPEGLLLPGMYVRAELEQGVRRQAVLAPQQGVARDDKG
RATALVLGAGDKVEQRLLTTEGTHGAYWLVTAGLKAGDRLIVDGLQRARPGEAAKPVALPAYAGRS
>Mature_386_residues
MVSVFAAGMHWNMRILTVVAMAASLAGCGAAERTPEAGKPPEVGVVRLEAKDVAIRAELAGRTVAYRSAEVRPQVNGIVL
KRHFTEGSLVKAGDLLYQIDPAGYQAALRQAEATLASARAALGSLKGRAERYAELAKIDGVSRQEREEADADYRKGLAAA
KAGEAAVAAARVDVSRTRVLAPISGRIGRSAVSEGALVTSGQSAALATIQQLDPMYVDIVQSSQDLLAFKRRQADGELEP
AGAAARLKLEDGAPYRHPGKLRFTELNVDRVSGAVTLRAEFPNPEGLLLPGMYVRAELEQGVRRQAVLAPQQGVARDDKG
RATALVLGAGDKVEQRLLTTEGTHGAYWLVTAGLKAGDRLIVDGLQRARPGEAAKPVALPAYAGRS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=362, Percent_Identity=49.171270718232, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1789665, Length=361, Percent_Identity=47.6454293628809, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1789929, Length=366, Percent_Identity=43.1693989071038, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI87082036, Length=314, Percent_Identity=30.5732484076433, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081951, Length=153, Percent_Identity=28.1045751633987, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40782; Mature: 40782

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVFAAGMHWNMRILTVVAMAASLAGCGAAERTPEAGKPPEVGVVRLEAKDVAIRAELA
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECC
GRTVAYRSAEVRPQVNGIVLKRHFTEGSLVKAGDLLYQIDPAGYQAALRQAEATLASARA
CCEEEEECCCCCCCCCCEEEEEECCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
ALGSLKGRAERYAELAKIDGVSRQEREEADADYRKGLAAAKAGEAAVAAARVDVSRTRVL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHCCCCHHHHHEEEEHHHHEEE
APISGRIGRSAVSEGALVTSGQSAALATIQQLDPMYVDIVQSSQDLLAFKRRQADGELEP
ECCCCCCCHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEHHCCHHHHHHHHHCCCCCCCC
AGAAARLKLEDGAPYRHPGKLRFTELNVDRVSGAVTLRAEFPNPEGLLLPGMYVRAELEQ
CCCEEEEEECCCCCCCCCCCEEEEEECHHHCCCEEEEEEECCCCCCEEECCHHHHHHHHH
GVRRQAVLAPQQGVARDDKGRATALVLGAGDKVEQRLLTTEGTHGAYWLVTAGLKAGDRL
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCCH
IVDGLQRARPGEAAKPVALPAYAGRS
HHHHHHHCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MVSVFAAGMHWNMRILTVVAMAASLAGCGAAERTPEAGKPPEVGVVRLEAKDVAIRAELA
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECC
GRTVAYRSAEVRPQVNGIVLKRHFTEGSLVKAGDLLYQIDPAGYQAALRQAEATLASARA
CCEEEEECCCCCCCCCCEEEEEECCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
ALGSLKGRAERYAELAKIDGVSRQEREEADADYRKGLAAAKAGEAAVAAARVDVSRTRVL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHCCCCHHHHHEEEEHHHHEEE
APISGRIGRSAVSEGALVTSGQSAALATIQQLDPMYVDIVQSSQDLLAFKRRQADGELEP
ECCCCCCCHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEHHCCHHHHHHHHHCCCCCCCC
AGAAARLKLEDGAPYRHPGKLRFTELNVDRVSGAVTLRAEFPNPEGLLLPGMYVRAELEQ
CCCEEEEEECCCCCCCCCCCEEEEEECHHHCCCEEEEEEECCCCCCEEECCHHHHHHHHH
GVRRQAVLAPQQGVARDDKGRATALVLGAGDKVEQRLLTTEGTHGAYWLVTAGLKAGDRL
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCCH
IVDGLQRARPGEAAKPVALPAYAGRS
HHHHHHHCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]