Click here to switch to the map view.

The map label for this gene is tsf

Identifier: 34497652

GI number: 34497652

Start: 2368239

End: 2369105

Strand: Direct

Name: tsf

Synonym: CV_2197

Alternate gene names: 34497652

Gene position: 2368239-2369105 (Clockwise)

Preceding gene: 34497651

Following gene: 34497653

Centisome position: 49.85

GC content: 61.71

Gene sequence:

>867_bases
ATGGCGGAAATCACCGCAAAAATGGTTTCGGATCTGCGCGTGGCCACCGGCCTCGGCATGATGGAGTGCAAGAAGGCTCT
GGTTGAAGCTGAAGGCGACTTCGCCAAGGCTGAAGAGATCCTGCGCATCAAGTCCGGCAGCAAGGCCTCCAAGATGGCCG
GCCGCCTGGCTGCCGAAGGCATCATCGGTTCCTACGTCGAAGGCGGCGTGGGCGCGCTGGTGGAAGTGAACTGCGAAACC
GACTTCGTGGCCAAGGATCCGACCTTCATCGCCCTGGCTAACGCTGCCGCCAAGGCCGTGGCCATCGCCAATCCGGCCGA
TGTGGAAGCCCTGGCCGCCGTTGAAGTCGACGGCCAACAAGTGGAAGAAATCCGCAAGGCTGCCATCGCCAAGCTGGGCG
AGAACATGACCATCCGCCGCTTCGTTCGCTACCAGACCGAAGGCGCGATCTCCACCTACCTGCACGGCGCCAAGATCGGC
GTGATCGTCGATTTCACCGGTCCGGAGCAAGTGGGCAAGGACGTCGCCATGCACGTCGCCGCCTCCAAGCCGATCTGCGT
GTCCAAGGACCAGGTTTCCGCCGAGACCCTGGACCAGGAACGCAAGATCTACAGCGCCCAGGCTGCCGAATCCGGCAAGC
CGGCCGACATCGTCGCCAAGATGGTTGAAGGCCGCATCAACAAGTTCCTGGCCGAGGTGACCCTGCTGGGTCAGCCCTTC
GTCAAGAACCCGGATGTGACCGTCGAGAAGCTGCTGGCTGAGCAGAAGGCTTCCGTCAAGGCGTTCGCCATGTTCGTCGT
AGGCGAAGGCATCGAGAAGAAAGTAGTAGACTACGCAGCTGAAGTGGCTGCGGCTGCCAAGCTGTAA

Upstream 100 bases:

>100_bases
GCTGCTGAAACGGCCCAGGCCGAGTAAGCGGAAAAGGGGCGAAAGCCCCTTTTTTTGGACCCGAACATTCACGTAATCTA
AATCATTAGGAGTTAGCAAG

Downstream 100 bases:

>100_bases
GCTCAGGCTTACCGCTTTATTGACGGCACCCTGCTTGCAGGGTGCCGTTTTGCAAACTGAACACACCACAAACCGCCAAT
CCGAGGACATCATGAGCCAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAEITAKMVSDLRVATGLGMMECKKALVEAEGDFAKAEEILRIKSGSKASKMAGRLAAEGIIGSYVEGGVGALVEVNCET
DFVAKDPTFIALANAAAKAVAIANPADVEALAAVEVDGQQVEEIRKAAIAKLGENMTIRRFVRYQTEGAISTYLHGAKIG
VIVDFTGPEQVGKDVAMHVAASKPICVSKDQVSAETLDQERKIYSAQAAESGKPADIVAKMVEGRINKFLAEVTLLGQPF
VKNPDVTVEKLLAEQKASVKAFAMFVVGEGIEKKVVDYAAEVAAAAKL

Sequences:

>Translated_288_residues
MAEITAKMVSDLRVATGLGMMECKKALVEAEGDFAKAEEILRIKSGSKASKMAGRLAAEGIIGSYVEGGVGALVEVNCET
DFVAKDPTFIALANAAAKAVAIANPADVEALAAVEVDGQQVEEIRKAAIAKLGENMTIRRFVRYQTEGAISTYLHGAKIG
VIVDFTGPEQVGKDVAMHVAASKPICVSKDQVSAETLDQERKIYSAQAAESGKPADIVAKMVEGRINKFLAEVTLLGQPF
VKNPDVTVEKLLAEQKASVKAFAMFVVGEGIEKKVVDYAAEVAAAAKL
>Mature_287_residues
AEITAKMVSDLRVATGLGMMECKKALVEAEGDFAKAEEILRIKSGSKASKMAGRLAAEGIIGSYVEGGVGALVEVNCETD
FVAKDPTFIALANAAAKAVAIANPADVEALAAVEVDGQQVEEIRKAAIAKLGENMTIRRFVRYQTEGAISTYLHGAKIGV
IVDFTGPEQVGKDVAMHVAASKPICVSKDQVSAETLDQERKIYSAQAAESGKPADIVAKMVEGRINKFLAEVTLLGQPFV
KNPDVTVEKLLAEQKASVKAFAMFVVGEGIEKKVVDYAAEVAAAAKL

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=301, Percent_Identity=28.2392026578073, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI291084500, Length=322, Percent_Identity=27.9503105590062, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI291084498, Length=97, Percent_Identity=41.2371134020619, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI291084502, Length=97, Percent_Identity=41.2371134020619, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786366, Length=288, Percent_Identity=49.3055555555556, Blast_Score=244, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17561440, Length=295, Percent_Identity=26.1016949152542, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921466, Length=285, Percent_Identity=29.1228070175439, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_CHRVO (Q7NVZ3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901867.1
- ProteinModelPortal:   Q7NVZ3
- SMR:   Q7NVZ3
- GeneID:   2549216
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2197
- NMPDR:   fig|243365.1.peg.2197
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   Q7NVZ3
- ProtClustDB:   PRK09377
- BioCyc:   CVIO243365:CV_2197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30359; Mature: 30228

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITAKMVSDLRVATGLGMMECKKALVEAEGDFAKAEEILRIKSGSKASKMAGRLAAEG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
IIGSYVEGGVGALVEVNCETDFVAKDPTFIALANAAAKAVAIANPADVEALAAVEVDGQQ
HHHHHHCCCCCEEEEEECCCCEEECCCCEEEEECHHHHEEEECCCCCCCEEEEEECCHHH
VEEIRKAAIAKLGENMTIRRFVRYQTEGAISTYLHGAKIGVIVDFTGPEQVGKDVAMHVA
HHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHH
ASKPICVSKDQVSAETLDQERKIYSAQAAESGKPADIVAKMVEGRINKFLAEVTLLGQPF
CCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VKNPDVTVEKLLAEQKASVKAFAMFVVGEGIEKKVVDYAAEVAAAAKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEITAKMVSDLRVATGLGMMECKKALVEAEGDFAKAEEILRIKSGSKASKMAGRLAAEG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
IIGSYVEGGVGALVEVNCETDFVAKDPTFIALANAAAKAVAIANPADVEALAAVEVDGQQ
HHHHHHCCCCCEEEEEECCCCEEECCCCEEEEECHHHHEEEECCCCCCCEEEEEECCHHH
VEEIRKAAIAKLGENMTIRRFVRYQTEGAISTYLHGAKIGVIVDFTGPEQVGKDVAMHVA
HHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHH
ASKPICVSKDQVSAETLDQERKIYSAQAAESGKPADIVAKMVEGRINKFLAEVTLLGQPF
CCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VKNPDVTVEKLLAEQKASVKAFAMFVVGEGIEKKVVDYAAEVAAAAKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782