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The map label for this gene is pdxJ

Identifier: 34497525

GI number: 34497525

Start: 2256419

End: 2257147

Strand: Direct

Name: pdxJ

Synonym: CV_2070

Alternate gene names: 34497525

Gene position: 2256419-2257147 (Clockwise)

Preceding gene: 34497524

Following gene: 34497526

Centisome position: 47.49

GC content: 67.63

Gene sequence:

>729_bases
ATGATTTTGTTGGGCGTAAACATCGATCACGTCGCCACGCTGCGCCAGGCGCGCGGCACCCGCTATCCCAGCCCGGTGGA
AGCCGCGCTGGTGGCGGAGTCCAGCGGCGCCGACCTGATCACGCTGCATTTGCGCGAGGATCGCCGCCACATCCAGGACG
CCGACGTCAAGGCGATGCGCCCGGTGCTGAAGACGCGGATGAACCTGGAAATGGCGATGACGCCGGAGATGCTGGCGCAC
GCGCTGCAGGTGGCGCCGGAAGACGTGTGCCTGGTGCCGGAAAAGCGCGAGGAAGTCACCACCGAAGGCGGCCTGGATGT
GCGCGGCCACTACGCCGACGTCAAGCATTACACCGGCAAGCTGACCGAGGCCGGCATCCGCGTGTCCATCTTCATCGACC
CGGACCGCGAGCAGATCCAGAAGGCGTTTGACGCCGGCGCGCGCGTGATCGAGCTGCACACCGGCGCTTACGCCGACGCG
CCGCACGCCAGCGAGCGCGAAGCCGAGCTGGCCCGCATCCGCGAGGCCGCCGAATTCGGCGCCAGCCTCGGCATGGTGGT
CAACGCCGGCCACGGACTCAATTACCACAACGTCAAGCCCATCGCCGCGATTCCGCAGATCGCCGAGCTGAATATCGGGC
ACGCCATCGTCGCCCATGCGCTGTTCGTCGGCTTCCCGCAGGCGGTGCGCGAGATGAAGGCGCTGATGGAAAGCGCGCGC
GCCCGCTGA

Upstream 100 bases:

>100_bases
CTGTCCGCGCTGCTGGGCGACGAGCCGCTGGCGTCGCGCCAATTATTGCAGGCCATCCAGTCCCTTTCGGACTGATGGCG
GATAGACAAGGGGGATGGAA

Downstream 100 bases:

>100_bases
TGCCGACGGTCGTCACCCACGCGCTGGCCGGCGCCACGCTGGTCGGCTTGGCCTGCCGCAAGCCGTTGTCCGCGCCCGGC
GTTTTGCCGTGGCTCGCGCT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MILLGVNIDHVATLRQARGTRYPSPVEAALVAESSGADLITLHLREDRRHIQDADVKAMRPVLKTRMNLEMAMTPEMLAH
ALQVAPEDVCLVPEKREEVTTEGGLDVRGHYADVKHYTGKLTEAGIRVSIFIDPDREQIQKAFDAGARVIELHTGAYADA
PHASEREAELARIREAAEFGASLGMVVNAGHGLNYHNVKPIAAIPQIAELNIGHAIVAHALFVGFPQAVREMKALMESAR
AR

Sequences:

>Translated_242_residues
MILLGVNIDHVATLRQARGTRYPSPVEAALVAESSGADLITLHLREDRRHIQDADVKAMRPVLKTRMNLEMAMTPEMLAH
ALQVAPEDVCLVPEKREEVTTEGGLDVRGHYADVKHYTGKLTEAGIRVSIFIDPDREQIQKAFDAGARVIELHTGAYADA
PHASEREAELARIREAAEFGASLGMVVNAGHGLNYHNVKPIAAIPQIAELNIGHAIVAHALFVGFPQAVREMKALMESAR
AR
>Mature_242_residues
MILLGVNIDHVATLRQARGTRYPSPVEAALVAESSGADLITLHLREDRRHIQDADVKAMRPVLKTRMNLEMAMTPEMLAH
ALQVAPEDVCLVPEKREEVTTEGGLDVRGHYADVKHYTGKLTEAGIRVSIFIDPDREQIQKAFDAGARVIELHTGAYADA
PHASEREAELARIREAAEFGASLGMVVNAGHGLNYHNVKPIAAIPQIAELNIGHAIVAHALFVGFPQAVREMKALMESAR
AR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=239, Percent_Identity=62.7615062761506, Blast_Score=298, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_CHRVO (Q7NWC0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901740.1
- ProteinModelPortal:   Q7NWC0
- SMR:   Q7NWC0
- GeneID:   2547412
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2070
- NMPDR:   fig|243365.1.peg.2070
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q7NWC0
- ProtClustDB:   PRK05265
- BioCyc:   CVIO243365:CV_2070-MONOMER
- BRENDA:   2.6.99.2
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26455; Mature: 26455

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 191-191 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLGVNIDHVATLRQARGTRYPSPVEAALVAESSGADLITLHLREDRRHIQDADVKAMR
CEEEECCHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCEEEEEEEHHHHHHCCCCHHHHH
PVLKTRMNLEMAMTPEMLAHALQVAPEDVCLVPEKREEVTTEGGLDVRGHYADVKHYTGK
HHHHHHCCCCEECCHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHCCC
LTEAGIRVSIFIDPDREQIQKAFDAGARVIELHTGAYADAPHASEREAELARIREAAEFG
CCCCCEEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
ASLGMVVNAGHGLNYHNVKPIAAIPQIAELNIGHAIVAHALFVGFPQAVREMKALMESAR
CCCCEEEECCCCCCCCCCCCHHHCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHC
AR
CC
>Mature Secondary Structure
MILLGVNIDHVATLRQARGTRYPSPVEAALVAESSGADLITLHLREDRRHIQDADVKAMR
CEEEECCHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCEEEEEEEHHHHHHCCCCHHHHH
PVLKTRMNLEMAMTPEMLAHALQVAPEDVCLVPEKREEVTTEGGLDVRGHYADVKHYTGK
HHHHHHCCCCEECCHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHCCC
LTEAGIRVSIFIDPDREQIQKAFDAGARVIELHTGAYADAPHASEREAELARIREAAEFG
CCCCCEEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
ASLGMVVNAGHGLNYHNVKPIAAIPQIAELNIGHAIVAHALFVGFPQAVREMKALMESAR
CCCCEEEECCCCCCCCCCCCHHHCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782