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The map label for this gene is aniA [H]

Identifier: 34497462

GI number: 34497462

Start: 2188174

End: 2189652

Strand: Direct

Name: aniA [H]

Synonym: CV_2007

Alternate gene names: 34497462

Gene position: 2188174-2189652 (Clockwise)

Preceding gene: 34497461

Following gene: 34497463

Centisome position: 46.06

GC content: 65.92

Gene sequence:

>1479_bases
TTGCTGCATTCGCTACCGGCAAGCCGATATCAGGCGAGGAGTGTTGCGGTGAAAAGATTGCTGAAGAATGGATTTTTCCT
GCCCGGCGTTTTGGCGGCGGCGTTGTCCTGTCTGGCGGCGCCGACGGGCGCCGGGGCCTTGCCGATTATTCATCAGGCGT
TGGTGGCGCCGCCGCTGCTGCCGCCGCCGATCACGCGCAAGACGCCGGCCCGGGTGGTGGTGGATTTGACGGTGGAGGAG
GTTGAGCGCGAAATCGCGCCGGGTTCTCGCTACATGTTCTGGACCTTCGGCGGCACGGTGCCGGGCAAGATGATACGGGT
GCGCGCCGGCGATACCGTGGAACTGCATCTGAAGAACCTGGCCGGCAACAAGCTGCCGCACAATATCGATTTGCACGCGG
TGACCGGCCCGGGCGGCGGGGCGCCGCAAACGCTGGTGGCGCCGGGCAATCAGGCGGTGTTCACCTTCAAGGCGCTGAAT
CCGGGGCTCTACGTCTACCATTGCGCCACCGCGCCGGTGGGCATGCACATCGCCAACGGCATGTACGGCATGATCCTGGT
GGAGCCGGAGAAGCCGCTGCCCAGGGTGGACCGCGAATATTACGTGATGCAGGGGGAGTTCTACACCGGCACCGGCTATC
GCGCCGGCGGGCTGCAGCCCTTCGACATGCAGAAGGCGATAGACGAAAAGCCCAGCTACGTGCTGTTCAACGGCGCCGAC
CGCGCGCTGGCTGGCAACCAGGCCTTGACCGCCAAGGCGGGCGAGAAGGTGAGGATCTATTTCGGCGTCGGCGGCCCCAA
CCTCACTTCCAGCTTCCACATCATCGGCGCGATCTTCGACAGGGTGTACAGCGAGGGCGGGGCCAATATCCAGCGCAATG
TGCAGACCACGATGGTGCCGGCCGGCGGCTCAGCCATCGTCGAATTCACGCCCAGAGTGCCGGGCAATCTGACCCTGGTG
GACCACTCGCTGACCCGCGCGTTCAACAAGGGCGCGATCGGCCTGCTGGCGGTGAGCGGGCCGGAGCGGCCGGACATCTA
TTCCAAGGGGGTGATCAGCGCGCTGGGCGCGACGGCGCCGGTTCAGGCCAAGCCTGCTCCTGGCGAAGAGGCGCCGGTCA
ACGCCGAGCCGCCGCAAACGCGCGAGCCGGCGCCGGCCGACCACGGCCGGGCGGTGTTCAGCAAGAACTGCGTGGTCTGC
CACCAGGCCAATGCCCAGGGTTTGGACGGCGTGTTTCCGCCCTTGGCCAACTCCGACCTGATCCAGAAGAATCCGCTTGA
GGCCGCCAAGATCATCCTGGGCGGCCGCGCCGGACCGATCACCGTCAACGGCGTCGGCTACAACGGCGTGATGCCGGCGC
TGGACCTGTCGGACCAGGAAGTGGCCGACGCGCTCAACTATGTCAGCACGCAATTCAACCATGGCAAGCCGGTGGTCACG
GTGGACGAGGTGAAGAAGCTGCGGGCGCAGCGGCAATGA

Upstream 100 bases:

>100_bases
TGAATAGACAATTCTTCAGAATTTAACATGTATTAATTATGCATTTTAAGGCGGGCATTGCGCCCACGGTGTCAGTCTGG
CGTGTCCGGCGGCTGCGTCC

Downstream 100 bases:

>100_bases
GCGCGAGGCTGGCGCTCGCGCTCCTGTGCTGCTGGCTGGCGGCAAGCCTGCCGGCGCGGGCGGCGGATTACGTCGCCGTG
CCCGGGGGGCCCTTCCGCAG

Product: nitrite reductase

Products: NA

Alternate protein names: Major outer membrane protein Pan 1 [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLHSLPASRYQARSVAVKRLLKNGFFLPGVLAAALSCLAAPTGAGALPIIHQALVAPPLLPPPITRKTPARVVVDLTVEE
VEREIAPGSRYMFWTFGGTVPGKMIRVRAGDTVELHLKNLAGNKLPHNIDLHAVTGPGGGAPQTLVAPGNQAVFTFKALN
PGLYVYHCATAPVGMHIANGMYGMILVEPEKPLPRVDREYYVMQGEFYTGTGYRAGGLQPFDMQKAIDEKPSYVLFNGAD
RALAGNQALTAKAGEKVRIYFGVGGPNLTSSFHIIGAIFDRVYSEGGANIQRNVQTTMVPAGGSAIVEFTPRVPGNLTLV
DHSLTRAFNKGAIGLLAVSGPERPDIYSKGVISALGATAPVQAKPAPGEEAPVNAEPPQTREPAPADHGRAVFSKNCVVC
HQANAQGLDGVFPPLANSDLIQKNPLEAAKIILGGRAGPITVNGVGYNGVMPALDLSDQEVADALNYVSTQFNHGKPVVT
VDEVKKLRAQRQ

Sequences:

>Translated_492_residues
MLHSLPASRYQARSVAVKRLLKNGFFLPGVLAAALSCLAAPTGAGALPIIHQALVAPPLLPPPITRKTPARVVVDLTVEE
VEREIAPGSRYMFWTFGGTVPGKMIRVRAGDTVELHLKNLAGNKLPHNIDLHAVTGPGGGAPQTLVAPGNQAVFTFKALN
PGLYVYHCATAPVGMHIANGMYGMILVEPEKPLPRVDREYYVMQGEFYTGTGYRAGGLQPFDMQKAIDEKPSYVLFNGAD
RALAGNQALTAKAGEKVRIYFGVGGPNLTSSFHIIGAIFDRVYSEGGANIQRNVQTTMVPAGGSAIVEFTPRVPGNLTLV
DHSLTRAFNKGAIGLLAVSGPERPDIYSKGVISALGATAPVQAKPAPGEEAPVNAEPPQTREPAPADHGRAVFSKNCVVC
HQANAQGLDGVFPPLANSDLIQKNPLEAAKIILGGRAGPITVNGVGYNGVMPALDLSDQEVADALNYVSTQFNHGKPVVT
VDEVKKLRAQRQ
>Mature_492_residues
MLHSLPASRYQARSVAVKRLLKNGFFLPGVLAAALSCLAAPTGAGALPIIHQALVAPPLLPPPITRKTPARVVVDLTVEE
VEREIAPGSRYMFWTFGGTVPGKMIRVRAGDTVELHLKNLAGNKLPHNIDLHAVTGPGGGAPQTLVAPGNQAVFTFKALN
PGLYVYHCATAPVGMHIANGMYGMILVEPEKPLPRVDREYYVMQGEFYTGTGYRAGGLQPFDMQKAIDEKPSYVLFNGAD
RALAGNQALTAKAGEKVRIYFGVGGPNLTSSFHIIGAIFDRVYSEGGANIQRNVQTTMVPAGGSAIVEFTPRVPGNLTLV
DHSLTRAFNKGAIGLLAVSGPERPDIYSKGVISALGATAPVQAKPAPGEEAPVNAEPPQTREPAPADHGRAVFSKNCVVC
HQANAQGLDGVFPPLANSDLIQKNPLEAAKIILGGRAGPITVNGVGYNGVMPALDLSDQEVADALNYVSTQFNHGKPVVT
VDEVKKLRAQRQ

Specific function: Catalyzes the reduction of nitrite to nitric oxide (NO), probably with azurin as electron donor. Essential for growth and survival in oxygen-depleted environments. Can also provide protection against killing by normal human sera [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 plastocyanin-like domains [H]

Homologues:

None

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011707
- InterPro:   IPR008972
- InterPro:   IPR001287 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07732 Cu-oxidase_3 [H]

EC number: =1.7.2.1 [H]

Molecular weight: Translated: 52038; Mature: 52038

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS51007 CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHSLPASRYQARSVAVKRLLKNGFFLPGVLAAALSCLAAPTGAGALPIIHQALVAPPLL
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
PPPITRKTPARVVVDLTVEEVEREIAPGSRYMFWTFGGTVPGKMIRVRAGDTVELHLKNL
CCCCCCCCCCEEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEEEECC
AGNKLPHNIDLHAVTGPGGGAPQTLVAPGNQAVFTFKALNPGLYVYHCATAPVGMHIANG
CCCCCCCCEEEEEEECCCCCCCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCEECCC
MYGMILVEPEKPLPRVDREYYVMQGEFYTGTGYRAGGLQPFDMQKAIDEKPSYVLFNGAD
EEEEEEECCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCHHHHHHHCCCCCEEEECCCC
RALAGNQALTAKAGEKVRIYFGVGGPNLTSSFHIIGAIFDRVYSEGGANIQRNVQTTMVP
CCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEEEE
AGGSAIVEFTPRVPGNLTLVDHSLTRAFNKGAIGLLAVSGPERPDIYSKGVISALGATAP
CCCCEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCC
VQAKPAPGEEAPVNAEPPQTREPAPADHGRAVFSKNCVVCHQANAQGLDGVFPPLANSDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCC
IQKNPLEAAKIILGGRAGPITVNGVGYNGVMPALDLSDQEVADALNYVSTQFNHGKPVVT
CCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEE
VDEVKKLRAQRQ
HHHHHHHHHCCC
>Mature Secondary Structure
MLHSLPASRYQARSVAVKRLLKNGFFLPGVLAAALSCLAAPTGAGALPIIHQALVAPPLL
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
PPPITRKTPARVVVDLTVEEVEREIAPGSRYMFWTFGGTVPGKMIRVRAGDTVELHLKNL
CCCCCCCCCCEEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEEEECC
AGNKLPHNIDLHAVTGPGGGAPQTLVAPGNQAVFTFKALNPGLYVYHCATAPVGMHIANG
CCCCCCCCEEEEEEECCCCCCCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCEECCC
MYGMILVEPEKPLPRVDREYYVMQGEFYTGTGYRAGGLQPFDMQKAIDEKPSYVLFNGAD
EEEEEEECCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCHHHHHHHCCCCCEEEECCCC
RALAGNQALTAKAGEKVRIYFGVGGPNLTSSFHIIGAIFDRVYSEGGANIQRNVQTTMVP
CCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEEEE
AGGSAIVEFTPRVPGNLTLVDHSLTRAFNKGAIGLLAVSGPERPDIYSKGVISALGATAP
CCCCEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCC
VQAKPAPGEEAPVNAEPPQTREPAPADHGRAVFSKNCVVCHQANAQGLDGVFPPLANSDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCC
IQKNPLEAAKIILGGRAGPITVNGVGYNGVMPALDLSDQEVADALNYVSTQFNHGKPVVT
CCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEE
VDEVKKLRAQRQ
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1383156; 1398981 [H]