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The map label for this gene is gltX

Identifier: 34497392

GI number: 34497392

Start: 2107938

End: 2109323

Strand: Direct

Name: gltX

Synonym: CV_1937

Alternate gene names: 34497392

Gene position: 2107938-2109323 (Clockwise)

Preceding gene: 34497391

Following gene: 34497396

Centisome position: 44.37

GC content: 66.31

Gene sequence:

>1386_bases
ATGATCCGTACCCGTTTCGCCCCCAGCCCCACCGGTTACCTGCACATCGGCGGCGTGCGCACCGCGCTGTTCTCCTGGGC
CTACGCCCGCAAAAACAACGGCGTGTTCGTGCTGCGCATCGAAGACACCGATCTGGAGCGCTCCACGCCGGAATCGGTGA
AGGCGATCCTGGACGGCATGCACTGGGTGGGTCTGGACTACGACGAGGGCCCGTTCTACCAGACCCATCGTTTCGACCGC
TACAAGGAAGTGATCCAGCAGCTGCTGGACAGCGGCCACGCCTACCACTGCTACTGCAGCAAGGAAGAGCTGGAGGCGAT
GCGCGCCGAGCAGGAAGCCCGCGGCGACAAGCCGCGCTATGACCGCCGCTGGCGTCCGGAAGCCGGCAAGACCCTGCCGG
CCATCCCGGAAGGCGTGCAGCCGGTGGTGCGCTTCAAGACCCCGCTCTCCGGCGTGGTGGCTTGGGACGACGCGGTCAAG
GGCCGCATCGAGATTTCCAACGAAGAGCTGGACGATCTGATCATCGCCCGCCCGGACGGCAGCCCCACCTACAACTTCTG
CGTGGTGGTGGACGACTGGGACATGCGGATCACCCACGTGATCCGCGGCGACGACCATGTCAACAACACCCCGCGCCAGA
TCAACATCCTCAAGGCGCTGAACGCGCCGCTGCCGGTGTACGGCCACCTGCCGATGATCCTGAACGAGGACGGCCAGAAG
ATGTCCAAGCGCCGCGACGCGGTCAGCGTGGTGGATTACGCCGACAAGGGCATCCTGCCGGAAGCGCTGCTGAACTACCT
GGCGCGCCTGGGCTGGGGCCACGGCGACGACGAATTCTTCAGCATGGAGCAGTTCGTCGAGTGGTTCAGCCTGGAGGCGG
TGAGCCCGTCGGCCAGCCGCTTCAACCACGAAAAATTCATGTGGCTGAACGCCCAGCACATCAAGGCCGCCGACAACGCC
CGCCTGGCCGAGCTGATCGCGCCGCGCCTGGCCGCCGCCCATGTCGACACCGCCCATGGCCCGGCCATCGGCGACGTGCT
GGCACTGGTCAAGGAGCGCGTGCAGGATCTGAACGCGCTGGCGCTGGAAGTGGATTACTTCTACAAGAAGCGCGAACCGG
CCGCCGCCGATGTGGAAAAGCATCTGGCCGGCGACGCCGTCGAGCGCATGGGCCGCTTCGCCGACCGTCTGGCCGCGCTG
GAAGCCTGGACCGCCGAATCGATCCACGAGCTGTTCAAGCCGTTCTGCGCCGACGAGGGCATCAAGATGGGCCAGCTGGG
CATGCCGCTGCGCGTGCTGGTGTGCGGCACCACCCAGACGCCGTCGGTTGACGCGGTGCTGGCGCTGATCGGCAAGGAAG
AAGTGCTGCGCCGCATCCGCGGCTGA

Upstream 100 bases:

>100_bases
TGATGGTTTGAGCCCCGATTTTCCCGAGCCCGCGTTTGCGGGCTTTTTTGTTGGCGGCGTACAATGACGCCTTTGTGTTT
TTCTCAGAGTCCAACCCATC

Downstream 100 bases:

>100_bases
TTCTGTCCGGCTGGCATCATCGCCCCCGCCGCCCTGATGGGCCGCGGGGGTTTTGTTTGCCCTCAGCCGGAGGCCAGCCG
CGCCAAGTAGCGCAGGCCGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIRTRFAPSPTGYLHIGGVRTALFSWAYARKNNGVFVLRIEDTDLERSTPESVKAILDGMHWVGLDYDEGPFYQTHRFDR
YKEVIQQLLDSGHAYHCYCSKEELEAMRAEQEARGDKPRYDRRWRPEAGKTLPAIPEGVQPVVRFKTPLSGVVAWDDAVK
GRIEISNEELDDLIIARPDGSPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALNAPLPVYGHLPMILNEDGQK
MSKRRDAVSVVDYADKGILPEALLNYLARLGWGHGDDEFFSMEQFVEWFSLEAVSPSASRFNHEKFMWLNAQHIKAADNA
RLAELIAPRLAAAHVDTAHGPAIGDVLALVKERVQDLNALALEVDYFYKKREPAAADVEKHLAGDAVERMGRFADRLAAL
EAWTAESIHELFKPFCADEGIKMGQLGMPLRVLVCGTTQTPSVDAVLALIGKEEVLRRIRG

Sequences:

>Translated_461_residues
MIRTRFAPSPTGYLHIGGVRTALFSWAYARKNNGVFVLRIEDTDLERSTPESVKAILDGMHWVGLDYDEGPFYQTHRFDR
YKEVIQQLLDSGHAYHCYCSKEELEAMRAEQEARGDKPRYDRRWRPEAGKTLPAIPEGVQPVVRFKTPLSGVVAWDDAVK
GRIEISNEELDDLIIARPDGSPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALNAPLPVYGHLPMILNEDGQK
MSKRRDAVSVVDYADKGILPEALLNYLARLGWGHGDDEFFSMEQFVEWFSLEAVSPSASRFNHEKFMWLNAQHIKAADNA
RLAELIAPRLAAAHVDTAHGPAIGDVLALVKERVQDLNALALEVDYFYKKREPAAADVEKHLAGDAVERMGRFADRLAAL
EAWTAESIHELFKPFCADEGIKMGQLGMPLRVLVCGTTQTPSVDAVLALIGKEEVLRRIRG
>Mature_461_residues
MIRTRFAPSPTGYLHIGGVRTALFSWAYARKNNGVFVLRIEDTDLERSTPESVKAILDGMHWVGLDYDEGPFYQTHRFDR
YKEVIQQLLDSGHAYHCYCSKEELEAMRAEQEARGDKPRYDRRWRPEAGKTLPAIPEGVQPVVRFKTPLSGVVAWDDAVK
GRIEISNEELDDLIIARPDGSPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALNAPLPVYGHLPMILNEDGQK
MSKRRDAVSVVDYADKGILPEALLNYLARLGWGHGDDEFFSMEQFVEWFSLEAVSPSASRFNHEKFMWLNAQHIKAADNA
RLAELIAPRLAAAHVDTAHGPAIGDVLALVKERVQDLNALALEVDYFYKKREPAAADVEKHLAGDAVERMGRFADRLAAL
EAWTAESIHELFKPFCADEGIKMGQLGMPLRVLVCGTTQTPSVDAVLALIGKEEVLRRIRG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=31.5151515151515, Blast_Score=225, Evalue=8e-59,
Organism=Homo sapiens, GI4826960, Length=294, Percent_Identity=27.5510204081633, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788743, Length=459, Percent_Identity=54.9019607843137, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=32.9824561403509, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=30.188679245283, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17543332, Length=120, Percent_Identity=30.8333333333333, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=483, Percent_Identity=30.2277432712215, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324742, Length=114, Percent_Identity=35.0877192982456, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=235, Percent_Identity=26.8085106382979, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=30.7070707070707, Blast_Score=228, Evalue=5e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHRVO (Q7NWP4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901607.1
- ProteinModelPortal:   Q7NWP4
- SMR:   Q7NWP4
- GeneID:   2550471
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1937
- NMPDR:   fig|243365.1.peg.1937
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q7NWP4
- ProtClustDB:   PRK01406
- BioCyc:   CVIO243365:CV_1937-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51914; Mature: 51914

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTRFAPSPTGYLHIGGVRTALFSWAYARKNNGVFVLRIEDTDLERSTPESVKAILDGM
CCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCCC
HWVGLDYDEGPFYQTHRFDRYKEVIQQLLDSGHAYHCYCSKEELEAMRAEQEARGDKPRY
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCC
DRRWRPEAGKTLPAIPEGVQPVVRFKTPLSGVVAWDDAVKGRIEISNEELDDLIIARPDG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCEEEECCCCCCCEEEECCCC
SPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALNAPLPVYGHLPMILNEDGQK
CCCEEEEEEEECCCCEEEEEEECCCCCCCCCHHEEHHHHHCCCCCCCCCCCCEECCCHHH
MSKRRDAVSVVDYADKGILPEALLNYLARLGWGHGDDEFFSMEQFVEWFSLEAVSPSASR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
FNHEKFMWLNAQHIKAADNARLAELIAPRLAAAHVDTAHGPAIGDVLALVKERVQDLNAL
CCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ALEVDYFYKKREPAAADVEKHLAGDAVERMGRFADRLAALEAWTAESIHELFKPFCADEG
EEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IKMGQLGMPLRVLVCGTTQTPSVDAVLALIGKEEVLRRIRG
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure
MIRTRFAPSPTGYLHIGGVRTALFSWAYARKNNGVFVLRIEDTDLERSTPESVKAILDGM
CCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCCC
HWVGLDYDEGPFYQTHRFDRYKEVIQQLLDSGHAYHCYCSKEELEAMRAEQEARGDKPRY
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCC
DRRWRPEAGKTLPAIPEGVQPVVRFKTPLSGVVAWDDAVKGRIEISNEELDDLIIARPDG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCEEEECCCCCCCEEEECCCC
SPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALNAPLPVYGHLPMILNEDGQK
CCCEEEEEEEECCCCEEEEEEECCCCCCCCCHHEEHHHHHCCCCCCCCCCCCEECCCHHH
MSKRRDAVSVVDYADKGILPEALLNYLARLGWGHGDDEFFSMEQFVEWFSLEAVSPSASR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
FNHEKFMWLNAQHIKAADNARLAELIAPRLAAAHVDTAHGPAIGDVLALVKERVQDLNAL
CCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ALEVDYFYKKREPAAADVEKHLAGDAVERMGRFADRLAALEAWTAESIHELFKPFCADEG
EEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IKMGQLGMPLRVLVCGTTQTPSVDAVLALIGKEEVLRRIRG
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782