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The map label for this gene is acsA1 [H]

Identifier: 34497213

GI number: 34497213

Start: 1912484

End: 1914445

Strand: Reverse

Name: acsA1 [H]

Synonym: CV_1758

Alternate gene names: 34497213

Gene position: 1914445-1912484 (Counterclockwise)

Preceding gene: 34497214

Following gene: 34497212

Centisome position: 40.29

GC content: 67.33

Gene sequence:

>1962_bases
ATGCAAGCACGCGTTACCCCGATCTGGAGCCCGTCCGCCGAACGGCTGGCCGGCTGCCATCTCACCGCCTTCGCCCGCCT
GGCCGAAACCGTCTGGGGCAAGGAATTGCCCGATTACCACCGCCTGTGGCGCGCCAGCGTGGAAGACCCGGGCCGCTTCT
GGTCGCTGGTCTGGGACTACTGCGGCGTGATCGGCGACAAGGGCGCCGTCGCGCTGGAGAACGGCGACGACATGCTGGCC
GCCCGCTTCTTCCCCGAGGCCCGGCTCAACTTCGCCGAAAACTTGCTGCGCCGCGACGACGACGCGCTGGCGGTGGTGTT
CCGCGGCGAGGACAAGATCGAACAAAAGCTGAGCTGGCATGAGCTGAACCAGCTGGTCTCCCGGCTGCAGCAGGCGATGC
GCGCCGCCGGCATCCAGCCGGGCGACCGCGTCGCCGGCTTCATGCCCAATATGCCGGCCACGCTGGCGGCGATGCTGGCC
GCCAGCAGCCTGGGCGCGGTGTGGACCAGCGGCTCTCCCGACTTCGGCACCGACGGCGCGCTGGACCGCTTCGGCCAGAC
CGAGCCCAGGATCCTGTTCTGCCCGGACGGCTACTGGTACAACGGCAAGGCGGTGGACATCCGCGCCAAGATGATCCACC
TGGCGGAAAAGCTGCCCAGCGTGGAGCGCTTCGTGGTGGTGCCTTACCTGGGCGACGGCGCCGACTTCGCCGCCGCCGTG
CCGCGCGCGCAGACGCTGGACGCCTTCCTGGCCGGTTTCGACGCCCGTCCGGTCGAATACCTCCGCCTGCCGTTCAACCA
TCCGCTGTACATCCTCTATTCCAGCGGCACCACCGGCAAGCCCAAGTGCATCGTCCACGGCGCCGGCGGCACGCTGCTGC
AGCACCTGAAGGAGCACCAGCTGCACGCCGACGTTCATGCCGGCGACCACCTGTTCTACTTCACCACCTGCGGCTGGATG
ATGTGGAACTGGCTGGTCAGCGGCCTGGCCTCCGGCGCCGCGCTGATGCTGTACGACGGCTCGCCGTTCGCCGACGAGGG
ACGCGTGCTGTGGGACTACGCCGCCGAGCACGGCTTCACCCATTTCGGCACCTCGGCCAAGTACATCGACAGCCTGAAGA
AGACGCCCATCGTCCCCGCGCGCGACTGGCAGCTGCCCAAGCTGCGCAGCCTGTTCTCCACCGGCTCGCCGCTGGTGGCG
GAAAGCTACGACTGGGTGTACGAGAACATCAAGTCCGACCTCAACCTCGCCTCCATTTCCGGCGGCACCGACATCGTGTC
CTGCTTCGCGCTGGGCGCCGCCACGCTGCCGGTCTACCGCGGCGAGCTGCAATGCCGCGGCCTGGGCATGGCGGTGGACA
TCTACGACGAGCTGGGCCGCCCGCTGTACCGCGAGAAAGGCGAGCTGGTTTGCCTGAAGCCGTTCCCGTCGATGCCGATA
GGCTTCTGGAACGACCCGGACGGCGAGAAATACCGCCAGGCCTACTTCGGCCGCTTCCCCAACATCTGGTGCCACGGCGA
CTACGCCGAAGTCACCCAGCACGACGGCGTGATCATCTACGGCCGCTCCGACGCGGTGCTCAACCCCGGCGGCGTGCGCA
TCGGCACCGCCGAGATCTACCGCCAGGTGGAAGTTTTCCCGGAAGTGCTGGAAAGCCTGGCCGTCGGCCAGACCTGGCAG
GACGACGAGCGCGTGGTGCTGTTCGTCAAGCTGCGCGAAGGCGTTCAGCTTGACGAGGATCTGAGCAGCCGGATCAAGGC
CCAGATCAAGAATGGGGCCAGCCCTCGCCACGTGCCGGCCCGCATCGTCGCCGTGGCCGACATCCCGCGCACCGTCAGCG
GCAAGATTGTCGAGCTGGCGGTGAAGAACATCATCCATGGCCGTCCGGTCAGCAACGTCAGCGCGCTGGCCAATCCGGAA
GCGCTGAAACTGTTCGAAAACCTGAGCGAGCTGGGAGAATGA

Upstream 100 bases:

>100_bases
TCGTTCCGCCGCCGCGCGCCGAGCGTTGACGGAGCCCGAATCATACCGGCCGGCTCGCCCTTCCAATCAAAAAACATCGC
GGGCCGCCACAGGAGAAATC

Downstream 100 bases:

>100_bases
GCGGCGACCACGCATGCCATTGACAAACGAGGGCCGCGCGCCCTCGTTTCCTTTCGCGCCACGGCAAGCACACGCGGAAA
ACCGCCGCGGGTTTTAAGCC

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MQARVTPIWSPSAERLAGCHLTAFARLAETVWGKELPDYHRLWRASVEDPGRFWSLVWDYCGVIGDKGAVALENGDDMLA
ARFFPEARLNFAENLLRRDDDALAVVFRGEDKIEQKLSWHELNQLVSRLQQAMRAAGIQPGDRVAGFMPNMPATLAAMLA
ASSLGAVWTSGSPDFGTDGALDRFGQTEPRILFCPDGYWYNGKAVDIRAKMIHLAEKLPSVERFVVVPYLGDGADFAAAV
PRAQTLDAFLAGFDARPVEYLRLPFNHPLYILYSSGTTGKPKCIVHGAGGTLLQHLKEHQLHADVHAGDHLFYFTTCGWM
MWNWLVSGLASGAALMLYDGSPFADEGRVLWDYAAEHGFTHFGTSAKYIDSLKKTPIVPARDWQLPKLRSLFSTGSPLVA
ESYDWVYENIKSDLNLASISGGTDIVSCFALGAATLPVYRGELQCRGLGMAVDIYDELGRPLYREKGELVCLKPFPSMPI
GFWNDPDGEKYRQAYFGRFPNIWCHGDYAEVTQHDGVIIYGRSDAVLNPGGVRIGTAEIYRQVEVFPEVLESLAVGQTWQ
DDERVVLFVKLREGVQLDEDLSSRIKAQIKNGASPRHVPARIVAVADIPRTVSGKIVELAVKNIIHGRPVSNVSALANPE
ALKLFENLSELGE

Sequences:

>Translated_653_residues
MQARVTPIWSPSAERLAGCHLTAFARLAETVWGKELPDYHRLWRASVEDPGRFWSLVWDYCGVIGDKGAVALENGDDMLA
ARFFPEARLNFAENLLRRDDDALAVVFRGEDKIEQKLSWHELNQLVSRLQQAMRAAGIQPGDRVAGFMPNMPATLAAMLA
ASSLGAVWTSGSPDFGTDGALDRFGQTEPRILFCPDGYWYNGKAVDIRAKMIHLAEKLPSVERFVVVPYLGDGADFAAAV
PRAQTLDAFLAGFDARPVEYLRLPFNHPLYILYSSGTTGKPKCIVHGAGGTLLQHLKEHQLHADVHAGDHLFYFTTCGWM
MWNWLVSGLASGAALMLYDGSPFADEGRVLWDYAAEHGFTHFGTSAKYIDSLKKTPIVPARDWQLPKLRSLFSTGSPLVA
ESYDWVYENIKSDLNLASISGGTDIVSCFALGAATLPVYRGELQCRGLGMAVDIYDELGRPLYREKGELVCLKPFPSMPI
GFWNDPDGEKYRQAYFGRFPNIWCHGDYAEVTQHDGVIIYGRSDAVLNPGGVRIGTAEIYRQVEVFPEVLESLAVGQTWQ
DDERVVLFVKLREGVQLDEDLSSRIKAQIKNGASPRHVPARIVAVADIPRTVSGKIVELAVKNIIHGRPVSNVSALANPE
ALKLFENLSELGE
>Mature_653_residues
MQARVTPIWSPSAERLAGCHLTAFARLAETVWGKELPDYHRLWRASVEDPGRFWSLVWDYCGVIGDKGAVALENGDDMLA
ARFFPEARLNFAENLLRRDDDALAVVFRGEDKIEQKLSWHELNQLVSRLQQAMRAAGIQPGDRVAGFMPNMPATLAAMLA
ASSLGAVWTSGSPDFGTDGALDRFGQTEPRILFCPDGYWYNGKAVDIRAKMIHLAEKLPSVERFVVVPYLGDGADFAAAV
PRAQTLDAFLAGFDARPVEYLRLPFNHPLYILYSSGTTGKPKCIVHGAGGTLLQHLKEHQLHADVHAGDHLFYFTTCGWM
MWNWLVSGLASGAALMLYDGSPFADEGRVLWDYAAEHGFTHFGTSAKYIDSLKKTPIVPARDWQLPKLRSLFSTGSPLVA
ESYDWVYENIKSDLNLASISGGTDIVSCFALGAATLPVYRGELQCRGLGMAVDIYDELGRPLYREKGELVCLKPFPSMPI
GFWNDPDGEKYRQAYFGRFPNIWCHGDYAEVTQHDGVIIYGRSDAVLNPGGVRIGTAEIYRQVEVFPEVLESLAVGQTWQ
DDERVVLFVKLREGVQLDEDLSSRIKAQIKNGASPRHVPARIVAVADIPRTVSGKIVELAVKNIIHGRPVSNVSALANPE
ALKLFENLSELGE

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=654, Percent_Identity=44.4954128440367, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI8923896, Length=687, Percent_Identity=25.9097525473071, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI253970489, Length=697, Percent_Identity=25.3945480631277, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI28416953, Length=600, Percent_Identity=24.6666666666667, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI13375727, Length=646, Percent_Identity=25.077399380805, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI115511026, Length=527, Percent_Identity=22.3908918406072, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1790505, Length=640, Percent_Identity=26.25, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1786529, Length=601, Percent_Identity=25.7903494176373, Blast_Score=140, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17569507, Length=656, Percent_Identity=37.5, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71983001, Length=684, Percent_Identity=24.4152046783626, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71982997, Length=677, Percent_Identity=24.519940915805, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319264, Length=647, Percent_Identity=26.4296754250386, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323182, Length=649, Percent_Identity=25.4237288135593, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24667955, Length=657, Percent_Identity=26.7884322678843, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI62472339, Length=561, Percent_Identity=28.3422459893048, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24667959, Length=524, Percent_Identity=28.2442748091603, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355225, Length=625, Percent_Identity=23.68, Blast_Score=126, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 72216; Mature: 72216

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARVTPIWSPSAERLAGCHLTAFARLAETVWGKELPDYHRLWRASVEDPGRFWSLVWDY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHH
CGVIGDKGAVALENGDDMLAARFFPEARLNFAENLLRRDDDALAVVFRGEDKIEQKLSWH
HHHCCCCCCEEEECCCCEEHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
ELNQLVSRLQQAMRAAGIQPGDRVAGFMPNMPATLAAMLAASSLGAVWTSGSPDFGTDGA
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCH
LDRFGQTEPRILFCPDGYWYNGKAVDIRAKMIHLAEKLPSVERFVVVPYLGDGADFAAAV
HHHCCCCCCEEEECCCCEEECCEEEEHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHC
PRAQTLDAFLAGFDARPVEYLRLPFNHPLYILYSSGTTGKPKCIVHGAGGTLLQHLKEHQ
CCHHHHHHHHHCCCCCCHHEEECCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHH
LHADVHAGDHLFYFTTCGWMMWNWLVSGLASGAALMLYDGSPFADEGRVLWDYAAEHGFT
HHEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEHHHHCCCC
HFGTSAKYIDSLKKTPIVPARDWQLPKLRSLFSTGSPLVAESYDWVYENIKSDLNLASIS
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHCCHHHHHHHHHCCCCEEECC
GGTDIVSCFALGAATLPVYRGELQCRGLGMAVDIYDELGRPLYREKGELVCLKPFPSMPI
CCHHHHHHHHHHHHHCCEECCCEEEECCCEEEEHHHHCCCCHHHCCCCEEEECCCCCCCC
GFWNDPDGEKYRQAYFGRFPNIWCHGDYAEVTQHDGVIIYGRSDAVLNPGGVRIGTAEIY
CCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHCCCCEEEECCCCCEECCCCCEEEHHHHH
RQVEVFPEVLESLAVGQTWQDDERVVLFVKLREGVQLDEDLSSRIKAQIKNGASPRHVPA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCE
RIVAVADIPRTVSGKIVELAVKNIIHGRPVSNVSALANPEALKLFENLSELGE
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQARVTPIWSPSAERLAGCHLTAFARLAETVWGKELPDYHRLWRASVEDPGRFWSLVWDY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHH
CGVIGDKGAVALENGDDMLAARFFPEARLNFAENLLRRDDDALAVVFRGEDKIEQKLSWH
HHHCCCCCCEEEECCCCEEHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
ELNQLVSRLQQAMRAAGIQPGDRVAGFMPNMPATLAAMLAASSLGAVWTSGSPDFGTDGA
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCH
LDRFGQTEPRILFCPDGYWYNGKAVDIRAKMIHLAEKLPSVERFVVVPYLGDGADFAAAV
HHHCCCCCCEEEECCCCEEECCEEEEHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHC
PRAQTLDAFLAGFDARPVEYLRLPFNHPLYILYSSGTTGKPKCIVHGAGGTLLQHLKEHQ
CCHHHHHHHHHCCCCCCHHEEECCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHH
LHADVHAGDHLFYFTTCGWMMWNWLVSGLASGAALMLYDGSPFADEGRVLWDYAAEHGFT
HHEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEHHHHCCCC
HFGTSAKYIDSLKKTPIVPARDWQLPKLRSLFSTGSPLVAESYDWVYENIKSDLNLASIS
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHCCHHHHHHHHHCCCCEEECC
GGTDIVSCFALGAATLPVYRGELQCRGLGMAVDIYDELGRPLYREKGELVCLKPFPSMPI
CCHHHHHHHHHHHHHCCEECCCEEEECCCEEEEHHHHCCCCHHHCCCCEEEECCCCCCCC
GFWNDPDGEKYRQAYFGRFPNIWCHGDYAEVTQHDGVIIYGRSDAVLNPGGVRIGTAEIY
CCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHCCCCEEEECCCCCEECCCCCEEEHHHHH
RQVEVFPEVLESLAVGQTWQDDERVVLFVKLREGVQLDEDLSSRIKAQIKNGASPRHVPA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCE
RIVAVADIPRTVSGKIVELAVKNIIHGRPVSNVSALANPEALKLFENLSELGE
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]