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The map label for this gene is ghrA [H]

Identifier: 34497179

GI number: 34497179

Start: 1864970

End: 1865896

Strand: Reverse

Name: ghrA [H]

Synonym: CV_1724

Alternate gene names: 34497179

Gene position: 1865896-1864970 (Counterclockwise)

Preceding gene: 34497182

Following gene: 34497175

Centisome position: 39.27

GC content: 68.07

Gene sequence:

>927_bases
ATGATCCTCGTACACACGCCCAATCCCAAGGAAGTCGCCGCCTATCTCGACCTGTTCCGCGCCGAGATGCCGGAACAGAC
CTTCGCCAGCCTGGAACATGGCGATTGCGCCGAAGCGCAGTACCTGATCGCCTGGGGTCTGCCCGATGGCCTGCTCGGCA
GGCTGCCCAAGCTGCGGGCGCTGTTCGCGCTGGGCGCGGGCGTGGACAAGCTACTGTCGCGGCCCGATCTGCCGGCCAGC
CTGCCGATCTACCGGCTGCTGGACGGCGGCATGGCGTCCCAGATGACCGAGTACATCCGCTACGGCGTGCTGGGTTACCA
GCGCAATATGGACATCTACCGCCGGCAGCAGGCGGCTGGACAGTGGCGGATGCTAGCGCCCCGCCTGCCCGACGAGATCC
GGGTCGGCATCCTGGGCCTGGGAGAGATCGGCTCGGCGGTGGCCAAGGCGCTGGCGAAGGACGGTTACCGGGTCAGCGGC
TGGAGCCGCGGCCCCAAGCATATCCACCACGTCCACAACCTGCATGGCGAAGCCGGGCTGGAAAAACTGCTGGAGAACAG
CGACGTGCTGGCCTGCGTGCTGCCTTCCACCCCGCAGACCCGTGGCCTGCTGAACGGCGAGCGGCTGTCGCTGATGCCCG
CAGGCGCCATGCTGATCAATGCCGGCCGGGGAGACCTGCTGGATCAGGATGCCCTGCTGGCCCTGCTCAACAACGGCCAC
ATCCGCTGCGCGCAACTGGACGTGTTCGCCGAAGAGCCCTTGCCGCACGGACATCCGCTGTGGAGCCATCCGTCGGTCGC
CGTCACCCCTCACATCGCCGCGATCACGCTGCGCCGCCAGGCCGTGGAACAGATCGCCGCCAATCTGCGCAAGCTCGCCG
CCGGGCAAGCGGCGGACGGCCGGGTGGAACGCGGCAACGGCTACTGA

Upstream 100 bases:

>100_bases
AATCTCAGGCCATCCGCAAGAACAGACCCGCAGCGGCAAAGCCTTGGCGGCCAGGCGCCGATGTATACTGGGGGCATAAT
CCCAACAAAGCGTCGCCGCC

Downstream 100 bases:

>100_bases
GCCCCTGCCCTGGCCGCCTGGACGGCGGCCTGTCCCAACATCCAGTTGACCCGCAAAGCATTCATACAGTCGTTTGATAA
GAATCTGAATTAAAAGGCAT

Product: phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MILVHTPNPKEVAAYLDLFRAEMPEQTFASLEHGDCAEAQYLIAWGLPDGLLGRLPKLRALFALGAGVDKLLSRPDLPAS
LPIYRLLDGGMASQMTEYIRYGVLGYQRNMDIYRRQQAAGQWRMLAPRLPDEIRVGILGLGEIGSAVAKALAKDGYRVSG
WSRGPKHIHHVHNLHGEAGLEKLLENSDVLACVLPSTPQTRGLLNGERLSLMPAGAMLINAGRGDLLDQDALLALLNNGH
IRCAQLDVFAEEPLPHGHPLWSHPSVAVTPHIAAITLRRQAVEQIAANLRKLAAGQAADGRVERGNGY

Sequences:

>Translated_308_residues
MILVHTPNPKEVAAYLDLFRAEMPEQTFASLEHGDCAEAQYLIAWGLPDGLLGRLPKLRALFALGAGVDKLLSRPDLPAS
LPIYRLLDGGMASQMTEYIRYGVLGYQRNMDIYRRQQAAGQWRMLAPRLPDEIRVGILGLGEIGSAVAKALAKDGYRVSG
WSRGPKHIHHVHNLHGEAGLEKLLENSDVLACVLPSTPQTRGLLNGERLSLMPAGAMLINAGRGDLLDQDALLALLNNGH
IRCAQLDVFAEEPLPHGHPLWSHPSVAVTPHIAAITLRRQAVEQIAANLRKLAAGQAADGRVERGNGY
>Mature_308_residues
MILVHTPNPKEVAAYLDLFRAEMPEQTFASLEHGDCAEAQYLIAWGLPDGLLGRLPKLRALFALGAGVDKLLSRPDLPAS
LPIYRLLDGGMASQMTEYIRYGVLGYQRNMDIYRRQQAAGQWRMLAPRLPDEIRVGILGLGEIGSAVAKALAKDGYRVSG
WSRGPKHIHHVHNLHGEAGLEKLLENSDVLACVLPSTPQTRGLLNGERLSLMPAGAMLINAGRGDLLDQDALLALLNNGH
IRCAQLDVFAEEPLPHGHPLWSHPSVAVTPHIAAITLRRQAVEQIAANLRKLAAGQAADGRVERGNGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=156, Percent_Identity=35.8974358974359, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI23308577, Length=172, Percent_Identity=33.7209302325581, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI145580578, Length=265, Percent_Identity=27.1698113207547, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4557499, Length=265, Percent_Identity=27.1698113207547, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=28.0155642023346, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=28.0155642023346, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI145580575, Length=265, Percent_Identity=27.1698113207547, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI87081824, Length=300, Percent_Identity=40, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082289, Length=248, Percent_Identity=31.8548387096774, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789279, Length=154, Percent_Identity=33.1168831168831, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=29.4820717131474, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324055, Length=249, Percent_Identity=27.3092369477912, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322116, Length=178, Percent_Identity=29.7752808988764, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=178, Percent_Identity=29.7752808988764, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325144, Length=183, Percent_Identity=28.4153005464481, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574286, Length=268, Percent_Identity=30.5970149253731, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28571528, Length=175, Percent_Identity=32, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574284, Length=165, Percent_Identity=35.1515151515151, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585514, Length=165, Percent_Identity=35.1515151515151, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28574282, Length=165, Percent_Identity=35.1515151515151, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI45551003, Length=165, Percent_Identity=35.1515151515151, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45552429, Length=165, Percent_Identity=35.1515151515151, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921140, Length=146, Percent_Identity=32.1917808219178, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33499; Mature: 33499

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVHTPNPKEVAAYLDLFRAEMPEQTFASLEHGDCAEAQYLIAWGLPDGLLGRLPKLRA
CEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCHHHHH
LFALGAGVDKLLSRPDLPASLPIYRLLDGGMASQMTEYIRYGVLGYQRNMDIYRRQQAAG
HHHHHCCHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
QWRMLAPRLPDEIRVGILGLGEIGSAVAKALAKDGYRVSGWSRGPKHIHHVHNLHGEAGL
CEEEECCCCCHHHEEEEEECHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCHHHH
EKLLENSDVLACVLPSTPQTRGLLNGERLSLMPAGAMLINAGRGDLLDQDALLALLNNGH
HHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHCCCC
IRCAQLDVFAEEPLPHGHPLWSHPSVAVTPHIAAITLRRQAVEQIAANLRKLAAGQAADG
EEEEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVERGNGY
CCCCCCCC
>Mature Secondary Structure
MILVHTPNPKEVAAYLDLFRAEMPEQTFASLEHGDCAEAQYLIAWGLPDGLLGRLPKLRA
CEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCHHHHH
LFALGAGVDKLLSRPDLPASLPIYRLLDGGMASQMTEYIRYGVLGYQRNMDIYRRQQAAG
HHHHHCCHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
QWRMLAPRLPDEIRVGILGLGEIGSAVAKALAKDGYRVSGWSRGPKHIHHVHNLHGEAGL
CEEEECCCCCHHHEEEEEECHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCHHHH
EKLLENSDVLACVLPSTPQTRGLLNGERLSLMPAGAMLINAGRGDLLDQDALLALLNNGH
HHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHCCCC
IRCAQLDVFAEEPLPHGHPLWSHPSVAVTPHIAAITLRRQAVEQIAANLRKLAAGQAADG
EEEEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVERGNGY
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]