Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 34497142

GI number: 34497142

Start: 1828489

End: 1829484

Strand: Direct

Name: ansA [H]

Synonym: CV_1687

Alternate gene names: 34497142

Gene position: 1828489-1829484 (Clockwise)

Preceding gene: 34497141

Following gene: 34497144

Centisome position: 38.49

GC content: 69.78

Gene sequence:

>996_bases
ATGAGCCGAATCCTGGTGCTCTACACCGGTGGTACCATAGGCATGGACCACACCCCTGAAGGCCTGGCCCCCGTGCCGGG
CCTACTGCCGCGCCTGCTGCAGCGTTTCGCCCGTCCAGGCCTGGAGTTCGATATCGTCGAATTCGAACAACTGATCGACT
CCTCGGCGCTGACGCCGCGCCAATGGAACCAGATACTGGACGCGCTGGTTTCCCGCTATCACGACTACGACGGTTTCGTC
GTCATCCACGGCACCGACACCATGGCCTACACCGCCGGCGTGCTGGCCTTCGCGCTGCGCGGCCTGGCCAAGCCTGTGGT
GCTGACCGGCGCGCAGCTGCCGTTGGTCCATCCGCGCTCCGATGGCTGGAGCAATCTGGCCGACGCGCTGGAAGCTGCCT
GCCAGCCTGACCTCAACGAAGTGGCGATCGCCTTCGACCGGCTGCTGCTGCGCGGCAGCCGCGCCCGCAAGCTGGATGTC
GAGCGTTTCGCCGGCTTCAACAGCCCCAACGCCGAGCCGCTGGCTGAGTTCGCCATCCACGCGCATTGGCGCAAGGAGCG
GTGGCTGAAGCCCGACGGCGAGTGGAATCCGCTGCGGATAGAAGAAAATCTGAAGCTGGCCGCGCTGATGCTGACGCCCG
GCCACGGCAGCGCGCTCGCCGGGCAGGTCTTGCGCGACGGCGGGCTGGATGGCGCCGTCCTGCTGGCTTACGGCAGCGGC
AACGCGCCGGCCGACCCGGTTCTGCTCGACGGCATCGCGGCGATGACCGCCCGCGGCTGCCCGGTATTGACGCTGACCCA
GGCGCCCAGCGGCGCGGTGGAGGTGGGCGCTTACGCCGCCAGCCAGCCGCTGGCCCGCGCCGGCGCGCTGCCGGGCGCGG
ACCTGACGCCGGAGGCCGCGCTGGCCAAGCTGCAGGCGCTGCTGTCGGCCGGCGTGTCCGGCGACGAGTTGCGCAAGCGG
CTGGTCCGCCCGCTGCGTGGCGAAATGACGGTCTGA

Upstream 100 bases:

>100_bases
GTCCGACGCGCTGCGCTGATCCGGTCTGTTCCGCCGGCGTGTGACGCCGGCTGAGGCTTGCGCTACCATCGTCCGCAGAC
CAAGTGCGATGGAGTATGCA

Downstream 100 bases:

>100_bases
GTTTTCTCTGTTGGAGTGAAAGGCTGCCTAAAAAATGGGCAGCCTTTTTTCATGCTTTCCGTTCAGAGGCTTGCGAGGCT
TTTCCACAACGCTTCCACAT

Product: L-asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSRILVLYTGGTIGMDHTPEGLAPVPGLLPRLLQRFARPGLEFDIVEFEQLIDSSALTPRQWNQILDALVSRYHDYDGFV
VIHGTDTMAYTAGVLAFALRGLAKPVVLTGAQLPLVHPRSDGWSNLADALEAACQPDLNEVAIAFDRLLLRGSRARKLDV
ERFAGFNSPNAEPLAEFAIHAHWRKERWLKPDGEWNPLRIEENLKLAALMLTPGHGSALAGQVLRDGGLDGAVLLAYGSG
NAPADPVLLDGIAAMTARGCPVLTLTQAPSGAVEVGAYAASQPLARAGALPGADLTPEAALAKLQALLSAGVSGDELRKR
LVRPLRGEMTV

Sequences:

>Translated_331_residues
MSRILVLYTGGTIGMDHTPEGLAPVPGLLPRLLQRFARPGLEFDIVEFEQLIDSSALTPRQWNQILDALVSRYHDYDGFV
VIHGTDTMAYTAGVLAFALRGLAKPVVLTGAQLPLVHPRSDGWSNLADALEAACQPDLNEVAIAFDRLLLRGSRARKLDV
ERFAGFNSPNAEPLAEFAIHAHWRKERWLKPDGEWNPLRIEENLKLAALMLTPGHGSALAGQVLRDGGLDGAVLLAYGSG
NAPADPVLLDGIAAMTARGCPVLTLTQAPSGAVEVGAYAASQPLARAGALPGADLTPEAALAKLQALLSAGVSGDELRKR
LVRPLRGEMTV
>Mature_330_residues
SRILVLYTGGTIGMDHTPEGLAPVPGLLPRLLQRFARPGLEFDIVEFEQLIDSSALTPRQWNQILDALVSRYHDYDGFVV
IHGTDTMAYTAGVLAFALRGLAKPVVLTGAQLPLVHPRSDGWSNLADALEAACQPDLNEVAIAFDRLLLRGSRARKLDVE
RFAGFNSPNAEPLAEFAIHAHWRKERWLKPDGEWNPLRIEENLKLAALMLTPGHGSALAGQVLRDGGLDGAVLLAYGSGN
APADPVLLDGIAAMTARGCPVLTLTQAPSGAVEVGAYAASQPLARAGALPGADLTPEAALAKLQALLSAGVSGDELRKRL
VRPLRGEMTV

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=40.1685393258427, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1788065, Length=335, Percent_Identity=41.7910447761194, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1789327, Length=271, Percent_Identity=27.6752767527675, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=364, Percent_Identity=36.2637362637363, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17558280, Length=364, Percent_Identity=36.2637362637363, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17558278, Length=364, Percent_Identity=36.2637362637363, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI193207374, Length=364, Percent_Identity=36.2637362637363, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24645530, Length=360, Percent_Identity=34.4444444444444, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=33.0601092896175, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35291; Mature: 35160

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILVLYTGGTIGMDHTPEGLAPVPGLLPRLLQRFARPGLEFDIVEFEQLIDSSALTPR
CCCEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCHH
QWNQILDALVSRYHDYDGFVVIHGTDTMAYTAGVLAFALRGLAKPVVLTGAQLPLVHPRS
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEECCCC
DGWSNLADALEAACQPDLNEVAIAFDRLLLRGSRARKLDVERFAGFNSPNAEPLAEFAIH
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHH
AHWRKERWLKPDGEWNPLRIEENLKLAALMLTPGHGSALAGQVLRDGGLDGAVLLAYGSG
HHHHHHCCCCCCCCCCCEEECCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCC
NAPADPVLLDGIAAMTARGCPVLTLTQAPSGAVEVGAYAASQPLARAGALPGADLTPEAA
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHCCCHHHHCCCCCCCCCHHHH
LAKLQALLSAGVSGDELRKRLVRPLRGEMTV
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRILVLYTGGTIGMDHTPEGLAPVPGLLPRLLQRFARPGLEFDIVEFEQLIDSSALTPR
CCEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCHH
QWNQILDALVSRYHDYDGFVVIHGTDTMAYTAGVLAFALRGLAKPVVLTGAQLPLVHPRS
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEECCCC
DGWSNLADALEAACQPDLNEVAIAFDRLLLRGSRARKLDVERFAGFNSPNAEPLAEFAIH
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHH
AHWRKERWLKPDGEWNPLRIEENLKLAALMLTPGHGSALAGQVLRDGGLDGAVLLAYGSG
HHHHHHCCCCCCCCCCCEEECCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCC
NAPADPVLLDGIAAMTARGCPVLTLTQAPSGAVEVGAYAASQPLARAGALPGADLTPEAA
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHCCCHHHHCCCCCCCCCHHHH
LAKLQALLSAGVSGDELRKRLVRPLRGEMTV
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]