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The map label for this gene is hcnB [H]

Identifier: 34497138

GI number: 34497138

Start: 1825123

End: 1826529

Strand: Reverse

Name: hcnB [H]

Synonym: CV_1683

Alternate gene names: 34497138

Gene position: 1826529-1825123 (Counterclockwise)

Preceding gene: 34497139

Following gene: 34497137

Centisome position: 38.44

GC content: 70.22

Gene sequence:

>1407_bases
ATGAGCGCCAAACCCGTCATCGTAGGCGGCGGCCCGGCCGGCATGTCGGCCGCCATGGAGCTGGCGCGCCACGGCGTGGA
GTGCGTGCTGCTGGACGAGGCCTCCCGCGTGGGCGGCGTGGTCTATCGCGGCCCGCTGCGGGAAGGCGTGGTGCTGGACT
ACCTGGGCGAGCGCTATCGCGAAAACATGCAGGCGCTGCACCGCGAATTCGAACAAGTCCGCGACCGCATCGACATCCGG
CTGAACACCCGCGTCGTCGGCGGCGGAGAGGGCGAACTGTTCGCGCTGACGCCGGACGAGCGAGTCGAGGCCGTCCCCTT
TTCCCAGCTGCTGCTGGCCGCCGGCTGCCATGAGCGCAGCGTGCCCTTCCCCGGCTGGACCCTGCCCGGCGTGATCATGC
TGGGCGGCCTGCAGCTTCAGATCAAGAGCGGCGTGGTCAAGCCGCTGGGGCGCACCGTGCTGGTCGGCACCGGCCCGCTG
TTGCCGCTGGTGGCGTGCCAGCTGCACCGCGCCGGCGTGGAAGTGGCCGGCGTTTACGAAGCCAGCGCCTTCGGCCGGCT
GGCCAAGGAAACGATCGCCCTGCTCAACCAACCGCAGCTGTTCCTGGACGGCCTGAGCATGGTGGCCTACCTGAAGCGCC
ACGGCATCCCGCTGCACTACGGCTGGGGCGTGGTCGAGGCCCACGGCGACGCCGAGCTGGGCGAAGTGACCGTCGCGCCC
TACGACGCGGAATGGCGCGCCGACCTGACGCGCAGCCGCCGCCTGCCGGCGCAGACGCTGGCCGTCGGCTACGGCTTCAT
CCCGCGCACCCAGCTGACCCAGCAGATGGGCCTGGAGCACCGCTACGCCGACGACGGCTACCTGAAGCCGGTCAACGACG
AATGGCAGCGCAGCAGCCGCGGCAACATCCACCTGGCCGGCGACATCGGCGGCCTGCGCGGCGGCGAAGCGGCGATGATC
AGCGGCCGCATCGCCGCGCTGTCCATGCTGCTGCAGCTGGGCAAGCTGGACGAGCAACAGGCGCTGCGCCAACGCCAGGC
ATGCCTGGACCAGATGGCGTCCATCCTGCGCTTCCGCAAGGGCATAGACAGCTTCACCCGCCGCGGCCGCGGCCAGTTGG
CGCTGCCGGCGCGCGACACCGTGATCTGCCGCTGCGAGCACGCCACCCGCCAGGACATCGACACCGCGCTGGAACAGGGC
GTCAACGACCTGATCAGCCTGAAGATGCGCACCCGGGTCAGCATGGGCGACTGCCAGGGCAAGATGTGCGTCGGCTATTG
CAGCGACCGCCTGCGCGAGCACACCGGCAAGCGCGACGTGGGCTGGATACGCCCGCGCTTCCCGCTGGACCCGCTGCCGT
TCTCGGCCTTCTCCGCCATCGACGCCCAACAGACTGAAGAGATTTGA

Upstream 100 bases:

>100_bases
AGATAGACGGCCGCCACAAGCGCCGCGCCTGCCAGACCGTGGTCCGCCCCGGCATGAAGGTGGAAACCGCCAGCAACCGT
CTGAATACGGAGGGTTTGCA

Downstream 100 bases:

>100_bases
CATGAGCAAGCAATACGACATCGTGATCGCCGGCGGCGGCGTGATCGGCGCCTCCTGCGCCTACCAGCTGTCCAAGCGCC
GCGATCTGAAAATCGCGCTG

Product: hydrogen cyanide synthase HcnB

Products: Dihydroxyacetone phosphate; Ubiquinol-8 [C]

Alternate protein names: Octopine oxidase subunit A [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSAKPVIVGGGPAGMSAAMELARHGVECVLLDEASRVGGVVYRGPLREGVVLDYLGERYRENMQALHREFEQVRDRIDIR
LNTRVVGGGEGELFALTPDERVEAVPFSQLLLAAGCHERSVPFPGWTLPGVIMLGGLQLQIKSGVVKPLGRTVLVGTGPL
LPLVACQLHRAGVEVAGVYEASAFGRLAKETIALLNQPQLFLDGLSMVAYLKRHGIPLHYGWGVVEAHGDAELGEVTVAP
YDAEWRADLTRSRRLPAQTLAVGYGFIPRTQLTQQMGLEHRYADDGYLKPVNDEWQRSSRGNIHLAGDIGGLRGGEAAMI
SGRIAALSMLLQLGKLDEQQALRQRQACLDQMASILRFRKGIDSFTRRGRGQLALPARDTVICRCEHATRQDIDTALEQG
VNDLISLKMRTRVSMGDCQGKMCVGYCSDRLREHTGKRDVGWIRPRFPLDPLPFSAFSAIDAQQTEEI

Sequences:

>Translated_468_residues
MSAKPVIVGGGPAGMSAAMELARHGVECVLLDEASRVGGVVYRGPLREGVVLDYLGERYRENMQALHREFEQVRDRIDIR
LNTRVVGGGEGELFALTPDERVEAVPFSQLLLAAGCHERSVPFPGWTLPGVIMLGGLQLQIKSGVVKPLGRTVLVGTGPL
LPLVACQLHRAGVEVAGVYEASAFGRLAKETIALLNQPQLFLDGLSMVAYLKRHGIPLHYGWGVVEAHGDAELGEVTVAP
YDAEWRADLTRSRRLPAQTLAVGYGFIPRTQLTQQMGLEHRYADDGYLKPVNDEWQRSSRGNIHLAGDIGGLRGGEAAMI
SGRIAALSMLLQLGKLDEQQALRQRQACLDQMASILRFRKGIDSFTRRGRGQLALPARDTVICRCEHATRQDIDTALEQG
VNDLISLKMRTRVSMGDCQGKMCVGYCSDRLREHTGKRDVGWIRPRFPLDPLPFSAFSAIDAQQTEEI
>Mature_467_residues
SAKPVIVGGGPAGMSAAMELARHGVECVLLDEASRVGGVVYRGPLREGVVLDYLGERYRENMQALHREFEQVRDRIDIRL
NTRVVGGGEGELFALTPDERVEAVPFSQLLLAAGCHERSVPFPGWTLPGVIMLGGLQLQIKSGVVKPLGRTVLVGTGPLL
PLVACQLHRAGVEVAGVYEASAFGRLAKETIALLNQPQLFLDGLSMVAYLKRHGIPLHYGWGVVEAHGDAELGEVTVAPY
DAEWRADLTRSRRLPAQTLAVGYGFIPRTQLTQQMGLEHRYADDGYLKPVNDEWQRSSRGNIHLAGDIGGLRGGEAAMIS
GRIAALSMLLQLGKLDEQQALRQRQACLDQMASILRFRKGIDSFTRRGRGQLALPARDTVICRCEHATRQDIDTALEQGV
NDLISLKMRTRVSMGDCQGKMCVGYCSDRLREHTGKRDVGWIRPRFPLDPLPFSAFSAIDAQQTEEI

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To T-protein and to dimethylglycine dehydrogenase [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR017224
- InterPro:   IPR000103 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF07992 Pyr_redox_2 [H]

EC number: 1.1.99.5 [C]

Molecular weight: Translated: 51321; Mature: 51190

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKPVIVGGGPAGMSAAMELARHGVECVLLDEASRVGGVVYRGPLREGVVLDYLGERYR
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHCCEEEECCCCCCHHHHHHHHHHH
ENMQALHREFEQVRDRIDIRLNTRVVGGGEGELFALTPDERVEAVPFSQLLLAAGCHERS
HHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCC
VPFPGWTLPGVIMLGGLQLQIKSGVVKPLGRTVLVGTGPLLPLVACQLHRAGVEVAGVYE
CCCCCCCCCHHHHCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEC
ASAFGRLAKETIALLNQPQLFLDGLSMVAYLKRHGIPLHYGWGVVEAHGDAELGEVTVAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCEEEECC
YDAEWRADLTRSRRLPAQTLAVGYGFIPRTQLTQQMGLEHRYADDGYLKPVNDEWQRSSR
CCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCC
GNIHLAGDIGGLRGGEAAMISGRIAALSMLLQLGKLDEQQALRQRQACLDQMASILRFRK
CCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIDSFTRRGRGQLALPARDTVICRCEHATRQDIDTALEQGVNDLISLKMRTRVSMGDCQG
HHHHHHHCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
KMCVGYCSDRLREHTGKRDVGWIRPRFPLDPLPFSAFSAIDAQQTEEI
CEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCC
>Mature Secondary Structure 
SAKPVIVGGGPAGMSAAMELARHGVECVLLDEASRVGGVVYRGPLREGVVLDYLGERYR
CCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHCCEEEECCCCCCHHHHHHHHHHH
ENMQALHREFEQVRDRIDIRLNTRVVGGGEGELFALTPDERVEAVPFSQLLLAAGCHERS
HHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCC
VPFPGWTLPGVIMLGGLQLQIKSGVVKPLGRTVLVGTGPLLPLVACQLHRAGVEVAGVYE
CCCCCCCCCHHHHCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEC
ASAFGRLAKETIALLNQPQLFLDGLSMVAYLKRHGIPLHYGWGVVEAHGDAELGEVTVAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCEEEECC
YDAEWRADLTRSRRLPAQTLAVGYGFIPRTQLTQQMGLEHRYADDGYLKPVNDEWQRSSR
CCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCC
GNIHLAGDIGGLRGGEAAMISGRIAALSMLLQLGKLDEQQALRQRQACLDQMASILRFRK
CCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIDSFTRRGRGQLALPARDTVICRCEHATRQDIDTALEQGVNDLISLKMRTRVSMGDCQG
HHHHHHHCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
KMCVGYCSDRLREHTGKRDVGWIRPRFPLDPLPFSAFSAIDAQQTEEI
CEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glycerol 3-phosphate; Ubiquinone-8 [C]

Specific reaction: Glycerol 3-phosphate + Ubiquinone-8 --> Dihydroxyacetone phosphate + Ubiquinol-8 [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA