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The map label for this gene is gloA [H]

Identifier: 34497115

GI number: 34497115

Start: 1798049

End: 1798438

Strand: Direct

Name: gloA [H]

Synonym: CV_1660

Alternate gene names: 34497115

Gene position: 1798049-1798438 (Clockwise)

Preceding gene: 34497114

Following gene: 34497116

Centisome position: 37.85

GC content: 63.59

Gene sequence:

>390_bases
ATGAGAATGCTTCACACCATGCTGCGCGTCGGCAACCTGGATCGCTCGATCGCCTTCTACCAGGAAGTGCTGGGCATGAA
GCTGCTGCGCCGCAACGACTTTCCGGAAGGCCGTTTCACGCTGGCCTTCGTCGGCTACGGCGACGAGGCCGAGCACACCG
TGCTGGAGCTGACCCACAACTGGGACACCGAATCCTACGATCTGGGCAGCGGCTATGGCCACATCGCCATCGAGGTGGAC
GACGCCTACGCCGCCTGTGACATGGCGCGCGCCAAGGGCGGCAAGGTGACGCGCGAGGCCGGCCCGATGAAGCACGGCAC
CACCGTGATCGCCTTCATCGAGGACCCGGACGGCTACAAGATCGAGTTCATCCAGAAAGGCAGCCGCTAA

Upstream 100 bases:

>100_bases
ACCGAGCGTCTCTGCCCCGGCTGGCGGGATGCCCGCCGCTGGCTGCGCCAGCACGGCGGCGGCTTGTTTGTCTTGGATTG
ATCTTTAAAAGGAAAACGCC

Downstream 100 bases:

>100_bases
GAGCGGCTAACAAAACCCCTGATGCTGCGTGGCGCCGGCTTGCCGTACGGCTTGTGCCGTCTGCGTCGGCGCGCCTTGCC
TCAGCCGTAAAACTGGGTTT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MRMLHTMLRVGNLDRSIAFYQEVLGMKLLRRNDFPEGRFTLAFVGYGDEAEHTVLELTHNWDTESYDLGSGYGHIAIEVD
DAYAACDMARAKGGKVTREAGPMKHGTTVIAFIEDPDGYKIEFIQKGSR

Sequences:

>Translated_129_residues
MRMLHTMLRVGNLDRSIAFYQEVLGMKLLRRNDFPEGRFTLAFVGYGDEAEHTVLELTHNWDTESYDLGSGYGHIAIEVD
DAYAACDMARAKGGKVTREAGPMKHGTTVIAFIEDPDGYKIEFIQKGSR
>Mature_129_residues
MRMLHTMLRVGNLDRSIAFYQEVLGMKLLRRNDFPEGRFTLAFVGYGDEAEHTVLELTHNWDTESYDLGSGYGHIAIEVD
DAYAACDMARAKGGKVTREAGPMKHGTTVIAFIEDPDGYKIEFIQKGSR

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=149, Percent_Identity=38.255033557047, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=65.0793650793651, Blast_Score=179, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=30.0751879699248, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=43.6619718309859, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=38, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=29.7101449275362, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14505; Mature: 14505

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGNLDRSIAFYQEVLGMKLLRRNDFPEGRFTLAFVGYGDEAEHTVLELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTESYDLGSGYGHIAIEVDDAYAACDMARAKGGKVTREAGPMKHGTTVIAFIEDPDGYK
CCCCCEECCCCCEEEEEEECCCHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCE
IEFIQKGSR
EEEEECCCC
>Mature Secondary Structure
MRMLHTMLRVGNLDRSIAFYQEVLGMKLLRRNDFPEGRFTLAFVGYGDEAEHTVLELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTESYDLGSGYGHIAIEVDDAYAACDMARAKGGKVTREAGPMKHGTTVIAFIEDPDGYK
CCCCCEECCCCCEEEEEEECCCHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCE
IEFIQKGSR
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]