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The map label for this gene is glyS [H]

Identifier: 34497111

GI number: 34497111

Start: 1793855

End: 1795915

Strand: Direct

Name: glyS [H]

Synonym: CV_1656

Alternate gene names: 34497111

Gene position: 1793855-1795915 (Clockwise)

Preceding gene: 34497110

Following gene: 34497112

Centisome position: 37.76

GC content: 69.19

Gene sequence:

>2061_bases
ATGAACGCGACCCTGCTGATCGAACTCCTGACCGAGGAGCTGCCGCCCAAGGCCCTGCCGCGTCTGGCCGCCAGTTTCGC
CCAGACCATCACCGAAGAACTGAAAAAGATGCAGTTCGTCGCCGCCGACGCCGCGGCGGTGACCTACGCGTCGCCGCGCC
GCCTGGCGGTGCAGCTGCCCGGCGTGCTCGCCGTGCAGCCGGACCAGCACATCACCCGCCAGGGTCCGGCCGCGGCCGCC
GGCATGAAGGACGGGCAGCCGACGCCGGCCCTGGCCGGCTTCGCCCGCTCCTGCGGCGTGGAAGTGTCAGAACTGTCCAT
CGTCAACAACGGCAAGCAGGACGTGTACGCCTATTCCAGCACCAAGCCGGGCGAGGCGCTGTCCGCGCAGCTGGCCGAGG
TGGTGTCGCTGGCGCTGAAGAAGCTGCCGGTGCCCAAGCTGATGCGCTGGGGCGATTCCGACGTGCAGTTCGTGCGCCCG
GTGCACGGCCTGATCATGTTGCACGGCGACAAGGTCATCGCTGGCGAGGTGCTGGGCCTGAACAGCGGCAACGCCACCCG
CGGCCACCGCTTCCTGTCGCAGGGCGAGGTCGTCGTCGCCAATGCCGACGCCTACGCCCGCGTTATGCATGAGCAGGGCA
AGGTGATCGCCAGCTTCGACGCCCGCCGCGAGCTGATCCGCCACAAGCTGGCCGAGGCCGCCGCCAAGCTCGGCGCCACC
ATCGCCGCGCCGGACGAGCTGTTCGACGAAGTGACCGCGCTGGTGGAATGGCCGGTGGTGCTGGAGGCCGGCTTCGAGGC
CGAGTTCCTGCAGGTGCCGCAGGAGTGCCTGATCCTGACCATGCAGCAGAACCAGAAGTACTTCCCGCTGCTGGACCGGA
ACGGCAAGCTGATGAACCGCTTCCTGCTGGTGTCCAATGTCGAGACCGCCGATCCGTCCTTCATCGTCGGCGGCAACGAG
CGCGTGCTGCGCGCCCGCCTGTCCGACGCCAAGTTCTTCTTCGAGCAGGACAAGAAGCACCGCCTGGACAGCCGCCTGCC
GCGCCTGGCCAACGTGGTGTACCACAACAAGATCGGCACGCAGCTGGAGCGCGTCGAGCGCCTGCAGAGCATCGCCGGCG
CCATCGCCCATCAGCTGGGCGCCGACGCCGCGCTGGCCGCCCGCGCCGCCTATCTGGCCAAGGCCGACCTGGTGACCGAC
ATGGTCGGCGAATTCCCGGAACTGCAGGGCATCATGGGCATGTACTACGCCCAGCACGACGGCGAGCACGCCGAAGTGGC
CGCCGCGGTGGAAGGGCATTACCATCCGCGCTTCGCCGGCGACACCCTGCCGGAAGGCAAGATCGCCACCGCGGTGGCGC
TGGCCGACAAGCTGGAGGCCATCGTCGGCATCTGGGGCATCGGCCTGATCCCGACCGGCGACAAGGATCCCTTCGCGCTG
CGCCGCGCCGCGCTGGGCGTGGTGCGCATGGCGCTGGAAGCCGACCTGGACCTGAAGCCGCTGCTGGCCATCGTCGCCGA
GGCCTTCCCGGCCGGCAAGCTGTCCGCCAACGTCGCCGACGAAGTGTTCGGCTTCATGATGGACCGCCTGAAGAACTTCC
TCGCCGCCGACTACAAGGGCGACGAGATCGACGCCGTGCTGGCGCTCTCGCCCAGCCGCCTGAACGAAGTGCGCGCGGTG
CTGGCCGCCGTGGCCGCGTTCAAGTCGCTGCCGGAAGCCGCCGCGCTGGCCGCCGCCAACAAGCGCGTCAACAACATCCT
GAAGAAGGCCGAAGGCGAAATCGGCGCGGTGGACGCCGCGCTGCTGCAGGAGGCCGCCGAGCAGGCGCTGTATGCCGCGG
TGCGGGATCTGGCGCCGGCGGTGGAGGTCAAGTTCGCCGCCCACGACTTCTCAGGCGCCTTGTCGCAGCTGGCCAGCCTG
AAAGCGCCGGTGGACGCGTTCTTCGACGGGGTGATGGTGATGGCCGAGGACGCCAGGGTGCGCGCCAACCGCATCGCGCT
GCTGGCCAGCCTGGCCGCGCTGTTCAACCGCGTGGCCGACATTTCGCTGCTGGCGGAGTGA

Upstream 100 bases:

>100_bases
GAAGTGGCAGCCGGTGAGCGAGGATTCGGTCGCCCACGCCATGATGAAGGTGATTTGCGAGGCGGCCGGAGCCGCATCCC
GATAATTGAAACGTTAGAGA

Downstream 100 bases:

>100_bases
GGGCAGGGCGGAGGCCCGGCTGTGCCGGGCGTTCCGCCAAGCTCGCAGTCGACGGGAATACAAACGGCGGAACGCCCCGG
CGGGGCTTCCGCCCTACTCA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MNATLLIELLTEELPPKALPRLAASFAQTITEELKKMQFVAADAAAVTYASPRRLAVQLPGVLAVQPDQHITRQGPAAAA
GMKDGQPTPALAGFARSCGVEVSELSIVNNGKQDVYAYSSTKPGEALSAQLAEVVSLALKKLPVPKLMRWGDSDVQFVRP
VHGLIMLHGDKVIAGEVLGLNSGNATRGHRFLSQGEVVVANADAYARVMHEQGKVIASFDARRELIRHKLAEAAAKLGAT
IAAPDELFDEVTALVEWPVVLEAGFEAEFLQVPQECLILTMQQNQKYFPLLDRNGKLMNRFLLVSNVETADPSFIVGGNE
RVLRARLSDAKFFFEQDKKHRLDSRLPRLANVVYHNKIGTQLERVERLQSIAGAIAHQLGADAALAARAAYLAKADLVTD
MVGEFPELQGIMGMYYAQHDGEHAEVAAAVEGHYHPRFAGDTLPEGKIATAVALADKLEAIVGIWGIGLIPTGDKDPFAL
RRAALGVVRMALEADLDLKPLLAIVAEAFPAGKLSANVADEVFGFMMDRLKNFLAADYKGDEIDAVLALSPSRLNEVRAV
LAAVAAFKSLPEAAALAAANKRVNNILKKAEGEIGAVDAALLQEAAEQALYAAVRDLAPAVEVKFAAHDFSGALSQLASL
KAPVDAFFDGVMVMAEDARVRANRIALLASLAALFNRVADISLLAE

Sequences:

>Translated_686_residues
MNATLLIELLTEELPPKALPRLAASFAQTITEELKKMQFVAADAAAVTYASPRRLAVQLPGVLAVQPDQHITRQGPAAAA
GMKDGQPTPALAGFARSCGVEVSELSIVNNGKQDVYAYSSTKPGEALSAQLAEVVSLALKKLPVPKLMRWGDSDVQFVRP
VHGLIMLHGDKVIAGEVLGLNSGNATRGHRFLSQGEVVVANADAYARVMHEQGKVIASFDARRELIRHKLAEAAAKLGAT
IAAPDELFDEVTALVEWPVVLEAGFEAEFLQVPQECLILTMQQNQKYFPLLDRNGKLMNRFLLVSNVETADPSFIVGGNE
RVLRARLSDAKFFFEQDKKHRLDSRLPRLANVVYHNKIGTQLERVERLQSIAGAIAHQLGADAALAARAAYLAKADLVTD
MVGEFPELQGIMGMYYAQHDGEHAEVAAAVEGHYHPRFAGDTLPEGKIATAVALADKLEAIVGIWGIGLIPTGDKDPFAL
RRAALGVVRMALEADLDLKPLLAIVAEAFPAGKLSANVADEVFGFMMDRLKNFLAADYKGDEIDAVLALSPSRLNEVRAV
LAAVAAFKSLPEAAALAAANKRVNNILKKAEGEIGAVDAALLQEAAEQALYAAVRDLAPAVEVKFAAHDFSGALSQLASL
KAPVDAFFDGVMVMAEDARVRANRIALLASLAALFNRVADISLLAE
>Mature_686_residues
MNATLLIELLTEELPPKALPRLAASFAQTITEELKKMQFVAADAAAVTYASPRRLAVQLPGVLAVQPDQHITRQGPAAAA
GMKDGQPTPALAGFARSCGVEVSELSIVNNGKQDVYAYSSTKPGEALSAQLAEVVSLALKKLPVPKLMRWGDSDVQFVRP
VHGLIMLHGDKVIAGEVLGLNSGNATRGHRFLSQGEVVVANADAYARVMHEQGKVIASFDARRELIRHKLAEAAAKLGAT
IAAPDELFDEVTALVEWPVVLEAGFEAEFLQVPQECLILTMQQNQKYFPLLDRNGKLMNRFLLVSNVETADPSFIVGGNE
RVLRARLSDAKFFFEQDKKHRLDSRLPRLANVVYHNKIGTQLERVERLQSIAGAIAHQLGADAALAARAAYLAKADLVTD
MVGEFPELQGIMGMYYAQHDGEHAEVAAAVEGHYHPRFAGDTLPEGKIATAVALADKLEAIVGIWGIGLIPTGDKDPFAL
RRAALGVVRMALEADLDLKPLLAIVAEAFPAGKLSANVADEVFGFMMDRLKNFLAADYKGDEIDAVLALSPSRLNEVRAV
LAAVAAFKSLPEAAALAAANKRVNNILKKAEGEIGAVDAALLQEAAEQALYAAVRDLAPAVEVKFAAHDFSGALSQLASL
KAPVDAFFDGVMVMAEDARVRANRIALLASLAALFNRVADISLLAE

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=686, Percent_Identity=46.6472303206997, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 73493; Mature: 73493

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATLLIELLTEELPPKALPRLAASFAQTITEELKKMQFVAADAAAVTYASPRRLAVQLP
CCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECC
GVLAVQPDQHITRQGPAAAAGMKDGQPTPALAGFARSCGVEVSELSIVNNGKQDVYAYSS
CEEEECCCHHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCCHHHEEEECCCCCCEEEECC
TKPGEALSAQLAEVVSLALKKLPVPKLMRWGDSDVQFVRPVHGLIMLHGDKVIAGEVLGL
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCEEEEEECCEEEEEEEEEC
NSGNATRGHRFLSQGEVVVANADAYARVMHEQGKVIASFDARRELIRHKLAEAAAKLGAT
CCCCCCHHHHHHCCCCEEEECCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
IAAPDELFDEVTALVEWPVVLEAGFEAEFLQVPQECLILTMQQNQKYFPLLDRNGKLMNR
CCCCHHHHHHHHHHHHCCHHEECCCCHHHHHCCHHHEEEECCCCCCCCCEECCCCHHHHH
FLLVSNVETADPSFIVGGNERVLRARLSDAKFFFEQDKKHRLDSRLPRLANVVYHNKIGT
HHEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLERVERLQSIAGAIAHQLGADAALAARAAYLAKADLVTDMVGEFPELQGIMGMYYAQHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
GEHAEVAAAVEGHYHPRFAGDTLPEGKIATAVALADKLEAIVGIWGIGLIPTGDKDPFAL
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHH
RRAALGVVRMALEADLDLKPLLAIVAEAFPAGKLSANVADEVFGFMMDRLKNFLAADYKG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DEIDAVLALSPSRLNEVRAVLAAVAAFKSLPEAAALAAANKRVNNILKKAEGEIGAVDAA
CCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLQEAAEQALYAAVRDLAPAVEVKFAAHDFSGALSQLASLKAPVDAFFDGVMVMAEDARV
HHHHHHHHHHHHHHHHHCHHHEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCHH
RANRIALLASLAALFNRVADISLLAE
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNATLLIELLTEELPPKALPRLAASFAQTITEELKKMQFVAADAAAVTYASPRRLAVQLP
CCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECC
GVLAVQPDQHITRQGPAAAAGMKDGQPTPALAGFARSCGVEVSELSIVNNGKQDVYAYSS
CEEEECCCHHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCCHHHEEEECCCCCCEEEECC
TKPGEALSAQLAEVVSLALKKLPVPKLMRWGDSDVQFVRPVHGLIMLHGDKVIAGEVLGL
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCEEEEEECCEEEEEEEEEC
NSGNATRGHRFLSQGEVVVANADAYARVMHEQGKVIASFDARRELIRHKLAEAAAKLGAT
CCCCCCHHHHHHCCCCEEEECCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
IAAPDELFDEVTALVEWPVVLEAGFEAEFLQVPQECLILTMQQNQKYFPLLDRNGKLMNR
CCCCHHHHHHHHHHHHCCHHEECCCCHHHHHCCHHHEEEECCCCCCCCCEECCCCHHHHH
FLLVSNVETADPSFIVGGNERVLRARLSDAKFFFEQDKKHRLDSRLPRLANVVYHNKIGT
HHEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLERVERLQSIAGAIAHQLGADAALAARAAYLAKADLVTDMVGEFPELQGIMGMYYAQHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
GEHAEVAAAVEGHYHPRFAGDTLPEGKIATAVALADKLEAIVGIWGIGLIPTGDKDPFAL
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHH
RRAALGVVRMALEADLDLKPLLAIVAEAFPAGKLSANVADEVFGFMMDRLKNFLAADYKG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DEIDAVLALSPSRLNEVRAVLAAVAAFKSLPEAAALAAANKRVNNILKKAEGEIGAVDAA
CCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLQEAAEQALYAAVRDLAPAVEVKFAAHDFSGALSQLASLKAPVDAFFDGVMVMAEDARV
HHHHHHHHHHHHHHHHHCHHHEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCHH
RANRIALLASLAALFNRVADISLLAE
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA