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The map label for this gene is hemB [H]

Identifier: 34497103

GI number: 34497103

Start: 1785639

End: 1786646

Strand: Direct

Name: hemB [H]

Synonym: CV_1648

Alternate gene names: 34497103

Gene position: 1785639-1786646 (Clockwise)

Preceding gene: 34497100

Following gene: 34497104

Centisome position: 37.58

GC content: 63.69

Gene sequence:

>1008_bases
ATGATTTTTGCCAATCGCTATTTCCCGGTGACCCGCATGCGCAGGATGCGCAAGGATGCGTTCAGCCGCCGGTTGATGCG
CGAGAACGTGCTGACCACCAACGACCTGATCTACCCGGTCTTCGTGATGGAGGGCAGCAATCAGGTGGTGGATGTGCCGT
CGATGCCGGGCGCGCCGCGGCTGACGCTGGACAAGCTGCTGCCGGTGGCCGAGGAGGCGCTGGAGCTGGGCATCCCGATG
CTGTCGCTGTTCCCGGTGATCACCGGGAACAAGGACAATGAAGGCAGCGAGGCCTACAATCCGGAAGGGCTGGTGCCGCG
CGTGGTGCGGGAGCTGAAGAAGCGCTTCCCGGAGCTGGGCGTGATGACCGACGGCGCGCTGGATCCTTATACCATCCACG
GCCAGGACGGCGTGATCGACGCCAACGGCTACGTGCTGAACGACGAGACCACGGAAATCCTGATCAAGCAGGGCCTGTGC
CATGCCGAAGCCGGCGTCGACGTGTTCGGCCCCTCCGACATGATGGACGGCCGCATCGGCGCGATCCGGGAGGCCTTCGA
GGAGGAAGGCTTCATCCACACCAAGATCCTGGCCTACTCCGCCAAGTATTCGTCCGGCTTCTACGGCCCGTTCCGCGACG
CGCTGGGCTCGTCCGCCAACCTCGGCAAGGCCGACAAGAACAGCTACCAGATGGATCCGGCCAATATCGAAGAGGCGATC
CAGGAGGTGGCCCAGGACTTGGAAGAGGGCGCCGACATGGTGATGATCAAGCCCGGCATGCCCTACCTGGACGTGATCCG
CCGCGTCAAGGACACCTTCCATGTGCCGACCTTCGCCTACCAGGTGTCCGGCGAGTATGCGATGCTGAAGGCGGCCTTCC
AGAACGGTTGGCTCAATGAAGAGAAGTGCATGATGGAGAGCCTGCTGGCGTTCAAGCGCGCCGGCGCCGACGGCATCCTC
ACTTATTTCGCGATGGACGCCGCCCGGCTGCTGCGCCAGCGCGGATAG

Upstream 100 bases:

>100_bases
CCTCGCTGCAGGAACGCATCGCGACGATGCGTAAAGCCCGCTGCCGCGTCGGGGGGATTGGGCGTACAATTGCCGATGTC
TTTTTTAGGGGGCTGTCGTC

Downstream 100 bases:

>100_bases
CCGATAATAGGTAGTGACGGACCGCACCCCTTGGCGCGAGCGTGGCAAGGGGTGTTCGCTTTTCGAGGATGAACCATGGA
CGTGCTGCATTCCACCTGGT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MIFANRYFPVTRMRRMRKDAFSRRLMRENVLTTNDLIYPVFVMEGSNQVVDVPSMPGAPRLTLDKLLPVAEEALELGIPM
LSLFPVITGNKDNEGSEAYNPEGLVPRVVRELKKRFPELGVMTDGALDPYTIHGQDGVIDANGYVLNDETTEILIKQGLC
HAEAGVDVFGPSDMMDGRIGAIREAFEEEGFIHTKILAYSAKYSSGFYGPFRDALGSSANLGKADKNSYQMDPANIEEAI
QEVAQDLEEGADMVMIKPGMPYLDVIRRVKDTFHVPTFAYQVSGEYAMLKAAFQNGWLNEEKCMMESLLAFKRAGADGIL
TYFAMDAARLLRQRG

Sequences:

>Translated_335_residues
MIFANRYFPVTRMRRMRKDAFSRRLMRENVLTTNDLIYPVFVMEGSNQVVDVPSMPGAPRLTLDKLLPVAEEALELGIPM
LSLFPVITGNKDNEGSEAYNPEGLVPRVVRELKKRFPELGVMTDGALDPYTIHGQDGVIDANGYVLNDETTEILIKQGLC
HAEAGVDVFGPSDMMDGRIGAIREAFEEEGFIHTKILAYSAKYSSGFYGPFRDALGSSANLGKADKNSYQMDPANIEEAI
QEVAQDLEEGADMVMIKPGMPYLDVIRRVKDTFHVPTFAYQVSGEYAMLKAAFQNGWLNEEKCMMESLLAFKRAGADGIL
TYFAMDAARLLRQRG
>Mature_335_residues
MIFANRYFPVTRMRRMRKDAFSRRLMRENVLTTNDLIYPVFVMEGSNQVVDVPSMPGAPRLTLDKLLPVAEEALELGIPM
LSLFPVITGNKDNEGSEAYNPEGLVPRVVRELKKRFPELGVMTDGALDPYTIHGQDGVIDANGYVLNDETTEILIKQGLC
HAEAGVDVFGPSDMMDGRIGAIREAFEEEGFIHTKILAYSAKYSSGFYGPFRDALGSSANLGKADKNSYQMDPANIEEAI
QEVAQDLEEGADMVMIKPGMPYLDVIRRVKDTFHVPTFAYQVSGEYAMLKAAFQNGWLNEEKCMMESLLAFKRAGADGIL
TYFAMDAARLLRQRG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=39.6103896103896, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI87081728, Length=325, Percent_Identity=44, Blast_Score=244, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6321398, Length=306, Percent_Identity=35.9477124183007, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21358291, Length=295, Percent_Identity=36.6101694915254, Blast_Score=204, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37320; Mature: 37320

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFANRYFPVTRMRRMRKDAFSRRLMRENVLTTNDLIYPVFVMEGSNQVVDVPSMPGAPR
CEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCC
LTLDKLLPVAEEALELGIPMLSLFPVITGNKDNEGSEAYNPEGLVPRVVRELKKRFPELG
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VMTDGALDPYTIHGQDGVIDANGYVLNDETTEILIKQGLCHAEAGVDVFGPSDMMDGRIG
CEECCCCCCEEECCCCCEEECCCEEECCCHHHHHHHHCCCHHHCCCCCCCCCHHCCCHHH
AIREAFEEEGFIHTKILAYSAKYSSGFYGPFRDALGSSANLGKADKNSYQMDPANIEEAI
HHHHHHHHCCCEEEEHEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
QEVAQDLEEGADMVMIKPGMPYLDVIRRVKDTFHVPTFAYQVSGEYAMLKAAFQNGWLNE
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCH
EKCMMESLLAFKRAGADGILTYFAMDAARLLRQRG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFANRYFPVTRMRRMRKDAFSRRLMRENVLTTNDLIYPVFVMEGSNQVVDVPSMPGAPR
CEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCCC
LTLDKLLPVAEEALELGIPMLSLFPVITGNKDNEGSEAYNPEGLVPRVVRELKKRFPELG
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VMTDGALDPYTIHGQDGVIDANGYVLNDETTEILIKQGLCHAEAGVDVFGPSDMMDGRIG
CEECCCCCCEEECCCCCEEECCCEEECCCHHHHHHHHCCCHHHCCCCCCCCCHHCCCHHH
AIREAFEEEGFIHTKILAYSAKYSSGFYGPFRDALGSSANLGKADKNSYQMDPANIEEAI
HHHHHHHHCCCEEEEHEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
QEVAQDLEEGADMVMIKPGMPYLDVIRRVKDTFHVPTFAYQVSGEYAMLKAAFQNGWLNE
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCH
EKCMMESLLAFKRAGADGILTYFAMDAARLLRQRG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]