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The map label for this gene is aceA [H]

Identifier: 34497096

GI number: 34497096

Start: 1776884

End: 1778182

Strand: Reverse

Name: aceA [H]

Synonym: CV_1641

Alternate gene names: 34497096

Gene position: 1778182-1776884 (Counterclockwise)

Preceding gene: 34497101

Following gene: 34497095

Centisome position: 37.43

GC content: 65.36

Gene sequence:

>1299_bases
ATGACGACTCGCGAACAACGCATTGCAGCCATCCAGAAGGACTGGGACGAAAACCCGCGCTGGAAGGGAATCAAGCGTGG
CTACAGCGCCGCCGACGTCGAACGCCTGCGCGGCTCGCTGCAGGTGGAGCACAGCCTGGCCCGCCATGGCGCGGAAAAAC
TGTGGCAGCTGGTCAACAACGAACCGTACATCAACTGTCTGGGCGCGCTGACCGGCGGCCAGGCGATGCAACAGGTGAAG
GCCGGCGTCAAGGCGATCTACCTGTCCGGCTGGCAGGTGGCCGCCGACAACAACGAATACTCGGCGATGTACCCGGACCA
GTCGCTGTACCCGGTGGATTCGGTGCCCAAGGTGGTGGAACGCATCAACAACGCCTTCACCCGCGCCGACGAGATCCAGC
ACGCGAAGGGCCTGGACAAGGGCGACAAGGGCTATGTCGACTACTTCGCCCCCATCGTCGCCGATGCCGAAGCCGGCTTC
GGCGGCGTGCTGAACGCGTATGAGCTGATGAAGGCGATGATCCGCGCCGGCGCCGCGGGCGTGCACTTCGAAGACCAGCT
GGCCTCGGTGAAGAAATGCGGCCACATGGGCGGCAAGGTGCTGGTGCCGACCCAGGAAGCGATCCAGAAGCTGATCGCCG
CCCGCCTGGCGGCCGACGTCTACGGCGTGCCGACCCTGGTGATCGCCCGCACCGACGCCGAAGCGGCCGACCTCTTGACC
AGCGACGTGGACCCGAACGACGAGCCCTTCCTGACCGGCGAACGCACCGCCGAAGGCTTCTACAAGACCAAGAAGGGCCT
GGAGCAGGCGATCAGCCGCGCGGTGGCCTACGCCGACTATGCCGACCTGGTGTGGTGCGAAACCGGCACGCCGGACCTGG
AGTTCGCCCGCAAGTTCGCCGAGGCCGTCCACGCCAAGCACCCGGGCAAGCTGCTGGCCTACAACTGCTCGCCGTCGTTC
AACTGGAAGAAGAACCTGGACGACGCCACCATCGCCAAGTTCCAGCGCGAGCTGGGCGCGATGGGCTACAAGTACCAGTT
CATCACCCTGGCCGGCATCCACAGCATGTGGTTCAACATGTACGACCTGGCGCAGGACTACGTGGCGCGCGGCATGTCCG
CATACGTCGAGAAGGTGCAGGAGCCCGAGTTCGCCGCCCGCGACCGCGGCTACAGCTTCGTGTCCCACCAGCAGGAAGTG
GGCACCGGCTACTTCGACGACGTCACCACCGTAATCCAGGGCGGAACCTCCAGCGTCACCGCGCTGACCGGCTCCACCGA
GGAAGAGCAGTTCCACTAA

Upstream 100 bases:

>100_bases
CCGGTTTCGACAAAAAATCCGGCACACCGGATACGCCGTACAGCCATCGAGGACGCCGGTTCGCCGGCGAGCACAGAAAC
GTTTCGAGAAGGGTTTCAAC

Downstream 100 bases:

>100_bases
AAGTGGGCGGCTGGCAGCCATGCCAGCCGCGTTGCCGCCACAAGCGGCCACTCATCAGCCCGCGTACCCCGCGGGCGGTG
CCGCCCCGTCTTACGGCCGG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTTREQRIAAIQKDWDENPRWKGIKRGYSAADVERLRGSLQVEHSLARHGAEKLWQLVNNEPYINCLGALTGGQAMQQVK
AGVKAIYLSGWQVAADNNEYSAMYPDQSLYPVDSVPKVVERINNAFTRADEIQHAKGLDKGDKGYVDYFAPIVADAEAGF
GGVLNAYELMKAMIRAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLIAARLAADVYGVPTLVIARTDAEAADLLT
SDVDPNDEPFLTGERTAEGFYKTKKGLEQAISRAVAYADYADLVWCETGTPDLEFARKFAEAVHAKHPGKLLAYNCSPSF
NWKKNLDDATIAKFQRELGAMGYKYQFITLAGIHSMWFNMYDLAQDYVARGMSAYVEKVQEPEFAARDRGYSFVSHQQEV
GTGYFDDVTTVIQGGTSSVTALTGSTEEEQFH

Sequences:

>Translated_432_residues
MTTREQRIAAIQKDWDENPRWKGIKRGYSAADVERLRGSLQVEHSLARHGAEKLWQLVNNEPYINCLGALTGGQAMQQVK
AGVKAIYLSGWQVAADNNEYSAMYPDQSLYPVDSVPKVVERINNAFTRADEIQHAKGLDKGDKGYVDYFAPIVADAEAGF
GGVLNAYELMKAMIRAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLIAARLAADVYGVPTLVIARTDAEAADLLT
SDVDPNDEPFLTGERTAEGFYKTKKGLEQAISRAVAYADYADLVWCETGTPDLEFARKFAEAVHAKHPGKLLAYNCSPSF
NWKKNLDDATIAKFQRELGAMGYKYQFITLAGIHSMWFNMYDLAQDYVARGMSAYVEKVQEPEFAARDRGYSFVSHQQEV
GTGYFDDVTTVIQGGTSSVTALTGSTEEEQFH
>Mature_431_residues
TTREQRIAAIQKDWDENPRWKGIKRGYSAADVERLRGSLQVEHSLARHGAEKLWQLVNNEPYINCLGALTGGQAMQQVKA
GVKAIYLSGWQVAADNNEYSAMYPDQSLYPVDSVPKVVERINNAFTRADEIQHAKGLDKGDKGYVDYFAPIVADAEAGFG
GVLNAYELMKAMIRAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLIAARLAADVYGVPTLVIARTDAEAADLLTS
DVDPNDEPFLTGERTAEGFYKTKKGLEQAISRAVAYADYADLVWCETGTPDLEFARKFAEAVHAKHPGKLLAYNCSPSFN
WKKNLDDATIAKFQRELGAMGYKYQFITLAGIHSMWFNMYDLAQDYVARGMSAYVEKVQEPEFAARDRGYSFVSHQQEVG
TGYFDDVTTVIQGGTSSVTALTGSTEEEQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=435, Percent_Identity=74.0229885057471, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=267, Percent_Identity=29.2134831460674, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17561814, Length=416, Percent_Identity=59.375, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71982926, Length=416, Percent_Identity=59.375, Blast_Score=506, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6320908, Length=291, Percent_Identity=36.0824742268041, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6325263, Length=253, Percent_Identity=39.1304347826087, Blast_Score=160, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47646; Mature: 47515

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTREQRIAAIQKDWDENPRWKGIKRGYSAADVERLRGSLQVEHSLARHGAEKLWQLVNN
CCCHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPYINCLGALTGGQAMQQVKAGVKAIYLSGWQVAADNNEYSAMYPDQSLYPVDSVPKVVE
CCCEEHHHHHCCHHHHHHHHHCHHEEEEECEEEEECCCCCEEECCCCCCCCCHHHHHHHH
RINNAFTRADEIQHAKGLDKGDKGYVDYFAPIVADAEAGFGGVLNAYELMKAMIRAGAAG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
VHFEDQLASVKKCGHMGGKVLVPTQEAIQKLIAARLAADVYGVPTLVIARTDAEAADLLT
CCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
SDVDPNDEPFLTGERTAEGFYKTKKGLEQAISRAVAYADYADLVWCETGTPDLEFARKFA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHH
EAVHAKHPGKLLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKYQFITLAGIHSMWFNM
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHH
YDLAQDYVARGMSAYVEKVQEPEFAARDRGYSFVSHQQEVGTGYFDDVTTVIQGGTSSVT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCEE
ALTGSTEEEQFH
EEECCCCCCCCC
>Mature Secondary Structure 
TTREQRIAAIQKDWDENPRWKGIKRGYSAADVERLRGSLQVEHSLARHGAEKLWQLVNN
CCHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPYINCLGALTGGQAMQQVKAGVKAIYLSGWQVAADNNEYSAMYPDQSLYPVDSVPKVVE
CCCEEHHHHHCCHHHHHHHHHCHHEEEEECEEEEECCCCCEEECCCCCCCCCHHHHHHHH
RINNAFTRADEIQHAKGLDKGDKGYVDYFAPIVADAEAGFGGVLNAYELMKAMIRAGAAG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
VHFEDQLASVKKCGHMGGKVLVPTQEAIQKLIAARLAADVYGVPTLVIARTDAEAADLLT
CCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
SDVDPNDEPFLTGERTAEGFYKTKKGLEQAISRAVAYADYADLVWCETGTPDLEFARKFA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHH
EAVHAKHPGKLLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKYQFITLAGIHSMWFNM
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHH
YDLAQDYVARGMSAYVEKVQEPEFAARDRGYSFVSHQQEVGTGYFDDVTTVIQGGTSSVT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCEE
ALTGSTEEEQFH
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]