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The map label for this gene is mltB [H]

Identifier: 34497064

GI number: 34497064

Start: 1736038

End: 1737078

Strand: Direct

Name: mltB [H]

Synonym: CV_1609

Alternate gene names: 34497064

Gene position: 1736038-1737078 (Clockwise)

Preceding gene: 34497063

Following gene: 34497065

Centisome position: 36.54

GC content: 68.01

Gene sequence:

>1041_bases
ATGAAATCGATGTTGAAACTGGCCATCGCCGCCGCCCTGGCCGGCGCGGCCCTCACCGCCCCGCCAGCCCAGGCTGACGC
CGCCTTCCTGTCTCGCCCCGATGTGCAGCGCTACATCGACGAGCAGGTGGCGAGCGGCCAGTTCAATCGCCCCGAACTGG
AGGCGGTGTTCGCCAATGTCGAGCTGAAGCCCAACATCATCCGCATCCTGGACAAGCCGTCCACCTCGCGGCCGTGGTAC
CAGTTCCGCGCGTCCACCGTCAACGACAAGCTGATCGCGGACGGAACCGCGTTCTGGCAGGCCAACGCCGACGCGCTGTC
CCGCGCCGAGCGCCAGTACGGCGTGGCGCCGGAGGTGATCGTCGCCATTCTGGGCGTGGAAACCCATTACGGCCGCAACA
CCGGCAGCTTCCGCCTGGTGGATTCCTTGTCCACCATCGGCTTCGACTACCCGCGCCGCGCCGCGTACTTCCGCGGCGAA
CTGACCCAGTTCCTGCTGCTGGCGAAGGAGGAGGGCAAGGACCCGCTGACGCTGAAGGGGTCCTACGCCGGCGCGATGGG
CCTGCCGCAGTTCATGCCGTCCAGCTACCGCAAGTGGGCGGTGGACTTTGACGGCAGCGGCCACCGCGACATCTGGAACA
ACCCGCATGACGCCATCGGCAGCGTCGCCCATTATTTCCAGCTGCACGGCTGGCGGCACGGCGACGACGTGGTGGCGCCG
GCCAGCGTGGCGCCAGGCGCCCAGGTCGACAAGCTGCTGGCCGACAAATTCAACTTGCACTACACGGTGGGCGAGCTGCG
CAAGCTGGGCGTGGCGCCGCAGGCGCAGCTGGCCGACGACGTGCCGGCGGTGCTGTTCTCGCTGGAGACCGCGCCCGGCG
TCACCGAATACTGGCTGGGCCTGAACAACTTCTACGCGATCACCCGCTACAACCGCAGCACGCTGTACGCCAAGGCGGTG
CAGGAAATCGCCGATCAGCTGCGCGCGCGCTACTACGCGACGCTGGCGCAGCGGCCGGACCCGGCGTCCGTCGCGCAGTA
A

Upstream 100 bases:

>100_bases
GAGCGGGTGATCCCCATCGTCGACATCACCGCCCGCCTGGTCAGCAACGCGCTGTCCTTCCGTGAATGAATCCGAGCCAT
ACCCAAAAGAAAGCAGAATC

Downstream 100 bases:

>100_bases
GGCCGGGCCCGCGCAACCGGCCACCGGCGCCGCCCGCGCGGCCGCGCCGGTGAAACCTGTCGCCTTGCGCGGCAATTCCC
GTTAAAATCCGCCCCCATGA

Product: peptidoglycan N-acetylmuramoylhydrolase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKSMLKLAIAAALAGAALTAPPAQADAAFLSRPDVQRYIDEQVASGQFNRPELEAVFANVELKPNIIRILDKPSTSRPWY
QFRASTVNDKLIADGTAFWQANADALSRAERQYGVAPEVIVAILGVETHYGRNTGSFRLVDSLSTIGFDYPRRAAYFRGE
LTQFLLLAKEEGKDPLTLKGSYAGAMGLPQFMPSSYRKWAVDFDGSGHRDIWNNPHDAIGSVAHYFQLHGWRHGDDVVAP
ASVAPGAQVDKLLADKFNLHYTVGELRKLGVAPQAQLADDVPAVLFSLETAPGVTEYWLGLNNFYAITRYNRSTLYAKAV
QEIADQLRARYYATLAQRPDPASVAQ

Sequences:

>Translated_346_residues
MKSMLKLAIAAALAGAALTAPPAQADAAFLSRPDVQRYIDEQVASGQFNRPELEAVFANVELKPNIIRILDKPSTSRPWY
QFRASTVNDKLIADGTAFWQANADALSRAERQYGVAPEVIVAILGVETHYGRNTGSFRLVDSLSTIGFDYPRRAAYFRGE
LTQFLLLAKEEGKDPLTLKGSYAGAMGLPQFMPSSYRKWAVDFDGSGHRDIWNNPHDAIGSVAHYFQLHGWRHGDDVVAP
ASVAPGAQVDKLLADKFNLHYTVGELRKLGVAPQAQLADDVPAVLFSLETAPGVTEYWLGLNNFYAITRYNRSTLYAKAV
QEIADQLRARYYATLAQRPDPASVAQ
>Mature_346_residues
MKSMLKLAIAAALAGAALTAPPAQADAAFLSRPDVQRYIDEQVASGQFNRPELEAVFANVELKPNIIRILDKPSTSRPWY
QFRASTVNDKLIADGTAFWQANADALSRAERQYGVAPEVIVAILGVETHYGRNTGSFRLVDSLSTIGFDYPRRAAYFRGE
LTQFLLLAKEEGKDPLTLKGSYAGAMGLPQFMPSSYRKWAVDFDGSGHRDIWNNPHDAIGSVAHYFQLHGWRHGDDVVAP
ASVAPGAQVDKLLADKFNLHYTVGELRKLGVAPQAQLADDVPAVLFSLETAPGVTEYWLGLNNFYAITRYNRSTLYAKAV
QEIADQLRARYYATLAQRPDPASVAQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=312, Percent_Identity=44.2307692307692, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 38077; Mature: 38077

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMLKLAIAAALAGAALTAPPAQADAAFLSRPDVQRYIDEQVASGQFNRPELEAVFANV
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECC
ELKPNIIRILDKPSTSRPWYQFRASTVNDKLIADGTAFWQANADALSRAERQYGVAPEVI
EECCCEEEEECCCCCCCCHHEEECCCCCCCEEECCCCEEECCHHHHHHHHHHHCCCHHHH
VAILGVETHYGRNTGSFRLVDSLSTIGFDYPRRAAYFRGELTQFLLLAKEEGKDPLTLKG
HHHHHCHHHCCCCCCCEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
SYAGAMGLPQFMPSSYRKWAVDFDGSGHRDIWNNPHDAIGSVAHYFQLHGWRHGDDVVAP
CCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ASVAPGAQVDKLLADKFNLHYTVGELRKLGVAPQAQLADDVPAVLFSLETAPGVTEYWLG
CCCCCCHHHHHHHHHHCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHC
LNNFYAITRYNRSTLYAKAVQEIADQLRARYYATLAQRPDPASVAQ
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKSMLKLAIAAALAGAALTAPPAQADAAFLSRPDVQRYIDEQVASGQFNRPELEAVFANV
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECC
ELKPNIIRILDKPSTSRPWYQFRASTVNDKLIADGTAFWQANADALSRAERQYGVAPEVI
EECCCEEEEECCCCCCCCHHEEECCCCCCCEEECCCCEEECCHHHHHHHHHHHCCCHHHH
VAILGVETHYGRNTGSFRLVDSLSTIGFDYPRRAAYFRGELTQFLLLAKEEGKDPLTLKG
HHHHHCHHHCCCCCCCEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
SYAGAMGLPQFMPSSYRKWAVDFDGSGHRDIWNNPHDAIGSVAHYFQLHGWRHGDDVVAP
CCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ASVAPGAQVDKLLADKFNLHYTVGELRKLGVAPQAQLADDVPAVLFSLETAPGVTEYWLG
CCCCCCHHHHHHHHHHCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHC
LNNFYAITRYNRSTLYAKAVQEIADQLRARYYATLAQRPDPASVAQ
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]