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The map label for this gene is cobQ

Identifier: 34497031

GI number: 34497031

Start: 1692796

End: 1694268

Strand: Direct

Name: cobQ

Synonym: CV_1576

Alternate gene names: 34497031

Gene position: 1692796-1694268 (Clockwise)

Preceding gene: 34497009

Following gene: 34497034

Centisome position: 35.63

GC content: 70.26

Gene sequence:

>1473_bases
ATGAGCGCCAAAACCATCATGATCCAGGGCGCCACGTCCGACGCCGGCAAGAGCGCGCTGGCCACCGGCCTGTGCCGGCT
GCTGGCCCGGCGCGGCGTGAAAGTGGCGCCGTTCAAGCCGCAGAACATGGCGCTGAACAGCGCGGTGACGGCGGACGGCG
GCGAGATCGGCCGCTCGCAGGCGGTGCAGGCGCAGGCCTGCGGCGTGGAGCCGCACACCGACATGAACCCGGTATTGCTC
AAGCCCAACAGCCACACCGGCTCGCAGGTGATCATCCAGGGCCGCGCCATCGGCAATATGGAGGCGCGCGGCTACCACGG
CTACAAGCCGGTCGCGATGAAGGCGGTGCTGGCGTCGCACGCCAGGCTGGCGGCGCAATACCAGTGCATCGTCGCCGAAG
GCGCCGGCAGTCCGGCCGAGATCAATCTGCGCGACGGCGACATCGCCAATATGGGCTTCGCCGAGGCGGTGGACTGCCCG
GTGATCCTGGTGGCCGACATCGAGCGCGGCGGCGTGTTCGCCCACCTGGCGGGCACGCTGGCGCTGCTGTCGGAATCCGA
GCGCGCCCGCGTGGTCGGCCTGGTGATCAACCGCTTCCGCGGCGATCCGTCCTTGCTCAAGACCGGCGTCGACTGGCTGG
AGCGGCACACCGGCAAGCCGGTGCTGGGCGTGCTGCCCTATCTGTACGACCTGCACCTGGAGGCCGAGGACAGCATGGGC
CTGGACAAGCCGGGCGGCGCTGTGGGCGGCGGCGACAAGCTGCGGGTGGCGGCGCCGGCCGCGCCGCGCGTCAGCAACCA
CACCGATTTCGATCCGTTGCGCCTGCACCCGCAGGTGGAATTCACCTTAGTGCGCGCCGGCCAAGCCATCCCGCCGGCCG
ACCTGATCGTTTTGCCCGGCAGCAAGAACGTGCTGGCGGACCTGGCCTGGCTGCGGGCGCAGGGCTGGGACGAGGCGCTG
CGCCGCCACCTGCGCTACGGCGGCAAGGTGCTGGGCATCTGCGGCGGCCTGCAGATGCTGGGCAAGAAGCTGCATGATCC
GGACGGGCTGGAAGGCGAGCCGGGCAGCGGCGACGGCCTGGGCTGGCTGGACATGGAAACCACGCTGGCGCCGGAGAAGC
GGCTGACGCGGATGCGCGGCCGGCTGACATTGGGCGACGCCGAGGCTGCCGGCTACGAAATCCACATGGGGGTCAGCCAA
GGGCCGGCCTTGGCCAGGCCCGCGGTGAGATTCGATGATGGCTCGGCCGACGGCGCGCGCTCGGAAGACGACCAGATCTT
CGCCACTTATCTGCACGGCGTGTTCGACGAACCGGCCGCCTGCCAGGCCATCCTGCGCTGGGCAGGGCTGAAGGAGCCGG
AGGCGCTGGACTACCCGGCGCTGCGCGAGGCCAATATCGACAAGCTGGCGGACATGCTGGAGCGGCATCTGGATCTGGAG
CCGATCCGGCGCTGGCTGCCCGAGACCCGCTGA

Upstream 100 bases:

>100_bases
GGAATTATCCAATGCGCAAGTCTGTCGCGCTTTGGGCGGCCGGAATGAATCAAGCCGCCATTCTAAGCGTTGTCAGCCAT
CCATGCGCAGGGAGATCGCC

Downstream 100 bases:

>100_bases
GCAAACCCCTGCGCTGCGCCTGCCGGTCGCACGACTTGCGCCGCCTGCGCCGGCGCGTCGTGGACCGGGCGCGGCGCAAG
CTTGGCCCAGCGGTGTTCAA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSAKTIMIQGATSDAGKSALATGLCRLLARRGVKVAPFKPQNMALNSAVTADGGEIGRSQAVQAQACGVEPHTDMNPVLL
KPNSHTGSQVIIQGRAIGNMEARGYHGYKPVAMKAVLASHARLAAQYQCIVAEGAGSPAEINLRDGDIANMGFAEAVDCP
VILVADIERGGVFAHLAGTLALLSESERARVVGLVINRFRGDPSLLKTGVDWLERHTGKPVLGVLPYLYDLHLEAEDSMG
LDKPGGAVGGGDKLRVAAPAAPRVSNHTDFDPLRLHPQVEFTLVRAGQAIPPADLIVLPGSKNVLADLAWLRAQGWDEAL
RRHLRYGGKVLGICGGLQMLGKKLHDPDGLEGEPGSGDGLGWLDMETTLAPEKRLTRMRGRLTLGDAEAAGYEIHMGVSQ
GPALARPAVRFDDGSADGARSEDDQIFATYLHGVFDEPAACQAILRWAGLKEPEALDYPALREANIDKLADMLERHLDLE
PIRRWLPETR

Sequences:

>Translated_490_residues
MSAKTIMIQGATSDAGKSALATGLCRLLARRGVKVAPFKPQNMALNSAVTADGGEIGRSQAVQAQACGVEPHTDMNPVLL
KPNSHTGSQVIIQGRAIGNMEARGYHGYKPVAMKAVLASHARLAAQYQCIVAEGAGSPAEINLRDGDIANMGFAEAVDCP
VILVADIERGGVFAHLAGTLALLSESERARVVGLVINRFRGDPSLLKTGVDWLERHTGKPVLGVLPYLYDLHLEAEDSMG
LDKPGGAVGGGDKLRVAAPAAPRVSNHTDFDPLRLHPQVEFTLVRAGQAIPPADLIVLPGSKNVLADLAWLRAQGWDEAL
RRHLRYGGKVLGICGGLQMLGKKLHDPDGLEGEPGSGDGLGWLDMETTLAPEKRLTRMRGRLTLGDAEAAGYEIHMGVSQ
GPALARPAVRFDDGSADGARSEDDQIFATYLHGVFDEPAACQAILRWAGLKEPEALDYPALREANIDKLADMLERHLDLE
PIRRWLPETR
>Mature_489_residues
SAKTIMIQGATSDAGKSALATGLCRLLARRGVKVAPFKPQNMALNSAVTADGGEIGRSQAVQAQACGVEPHTDMNPVLLK
PNSHTGSQVIIQGRAIGNMEARGYHGYKPVAMKAVLASHARLAAQYQCIVAEGAGSPAEINLRDGDIANMGFAEAVDCPV
ILVADIERGGVFAHLAGTLALLSESERARVVGLVINRFRGDPSLLKTGVDWLERHTGKPVLGVLPYLYDLHLEAEDSMGL
DKPGGAVGGGDKLRVAAPAAPRVSNHTDFDPLRLHPQVEFTLVRAGQAIPPADLIVLPGSKNVLADLAWLRAQGWDEALR
RHLRYGGKVLGICGGLQMLGKKLHDPDGLEGEPGSGDGLGWLDMETTLAPEKRLTRMRGRLTLGDAEAAGYEIHMGVSQG
PALARPAVRFDDGSADGARSEDDQIFATYLHGVFDEPAACQAILRWAGLKEPEALDYPALREANIDKLADMLERHLDLEP
IRRWLPETR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_CHRVO (Q7NXQ0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901246.1
- ProteinModelPortal:   Q7NXQ0
- SMR:   Q7NXQ0
- GeneID:   2551152
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1576
- NMPDR:   fig|243365.1.peg.1576
- HOGENOM:   HBG302271
- OMA:   AMSYRER
- ProtClustDB:   PRK00784
- BioCyc:   CVIO243365:CV_1576-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 52371; Mature: 52239

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKTIMIQGATSDAGKSALATGLCRLLARRGVKVAPFKPQNMALNSAVTADGGEIGRSQ
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEECCCHHCCHHH
AVQAQACGVEPHTDMNPVLLKPNSHTGSQVIIQGRAIGNMEARGYHGYKPVAMKAVLASH
HHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEECCEECCCCCCCCCCCCHHHHHHHHHHH
ARLAAQYQCIVAEGAGSPAEINLRDGDIANMGFAEAVDCPVILVADIERGGVFAHLAGTL
HHHHHEEEEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHH
ALLSESERARVVGLVINRFRGDPSLLKTGVDWLERHTGKPVLGVLPYLYDLHLEAEDSMG
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCC
LDKPGGAVGGGDKLRVAAPAAPRVSNHTDFDPLRLHPQVEFTLVRAGQAIPPADLIVLPG
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECC
SKNVLADLAWLRAQGWDEALRRHLRYGGKVLGICGGLQMLGKKLHDPDGLEGEPGSGDGL
CCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCE
GWLDMETTLAPEKRLTRMRGRLTLGDAEAAGYEIHMGVSQGPALARPAVRFDDGSADGAR
EEEECCHHCCCHHHHHHHHCCEEECCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCC
SEDDQIFATYLHGVFDEPAACQAILRWAGLKEPEALDYPALREANIDKLADMLERHLDLE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHH
PIRRWLPETR
HHHHHCCCCC
>Mature Secondary Structure 
SAKTIMIQGATSDAGKSALATGLCRLLARRGVKVAPFKPQNMALNSAVTADGGEIGRSQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEECCCHHCCHHH
AVQAQACGVEPHTDMNPVLLKPNSHTGSQVIIQGRAIGNMEARGYHGYKPVAMKAVLASH
HHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEECCEECCCCCCCCCCCCHHHHHHHHHHH
ARLAAQYQCIVAEGAGSPAEINLRDGDIANMGFAEAVDCPVILVADIERGGVFAHLAGTL
HHHHHEEEEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHH
ALLSESERARVVGLVINRFRGDPSLLKTGVDWLERHTGKPVLGVLPYLYDLHLEAEDSMG
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCC
LDKPGGAVGGGDKLRVAAPAAPRVSNHTDFDPLRLHPQVEFTLVRAGQAIPPADLIVLPG
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECC
SKNVLADLAWLRAQGWDEALRRHLRYGGKVLGICGGLQMLGKKLHDPDGLEGEPGSGDGL
CCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCE
GWLDMETTLAPEKRLTRMRGRLTLGDAEAAGYEIHMGVSQGPALARPAVRFDDGSADGAR
EEEECCHHCCCHHHHHHHHCCEEECCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCC
SEDDQIFATYLHGVFDEPAACQAILRWAGLKEPEALDYPALREANIDKLADMLERHLDLE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHH
PIRRWLPETR
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782