Click here to switch to the map view.

The map label for this gene is cobD

Identifier: 34497030

GI number: 34497030

Start: 1691724

End: 1692662

Strand: Reverse

Name: cobD

Synonym: CV_1575

Alternate gene names: 34497030

Gene position: 1692662-1691724 (Counterclockwise)

Preceding gene: 34497032

Following gene: 34497029

Centisome position: 35.63

GC content: 71.46

Gene sequence:

>939_bases
TTGAATTCCGCACTGTTTCTGCCGATCGCGCTGCTGCTGGACCGCTTGCTGGGCGAACCGCCGCGCTGGCACCCGCTGGT
CGGCTTCGGCCGGCTGGTCAAGGCCGTGGAGCGCGCGGCCTACCCGGCCGCGCCCGGCGCGGAGCCTGTCTGGCGCATGC
GGCTGCGCGGCGCGGCGGCCATCGCGCTGTTATTGGCGCCCTTCACGCTGGCCGCCTGGGCGCTGGCGAGGCTGCCGCTG
CTGGAGATTATCGTTCCGGTCGCCCTGCTCTACCTGGCGGTCGGCGCTCGCAGCCTGGCCCAGCACGCCGAGGTGGTGCG
CAAGGCCTTGGCCGAAGGCGACCTTCAACTTGCCCGCGAGCGGGTGGGCTGGATCGTCAGCCGCGACACCCGCGAACTGG
ACGAAGCCGGCGTCGCCCGCGCGACGATTGAGTCGGTGCTGGAAAACGGCAGCGACGCCGTCTTCGCCGCGCTGTTCTGG
TTCCTGGTCCTGGGCGCGCCCGGCGCGGTGCTGTACCGGCTGGCCAACACGCTGGACGCGATGTGGGGCTACAAGAACGA
GCGCTACCTGCATTTCGGCTGGGCCGCCGCCCGCTTCGACGACATGCTGAATTATCTCCCGGCGCGTCTGACCGCCCTCA
CCTATCTGCTGCTGGGCCATGCCGCCAAGGGCTGGCGCTGCTGGCGAACCCAGGCCCCGACCTGGTACAGCCCCAACGCC
GGCCCGGTGATGGCCGCCGGCGCCGGCGCGCTGGGCGTCAGCCTGGGCGGCGGCGCGCGCTATCACGGCCAGTGGAAGGA
ACGTCCGCCGCTTGGCTGCGGCCCCACGCCCACGCATGAAGACATCGGCCGCGCGGTGCGGCTGGTCAACCGCGGCATGT
GGCTGTGGGCGGCGTTGTCGCTGGCGGCCGCCATCTTGATCGGAGCGATCCATGCTTGA

Upstream 100 bases:

>100_bases
CTTAGAATGGCGGCTTGATTCATTCCGGCCGCCCAAAGCGCGACAGACTTGCGCATTGGATAATTCCCGGCGGCGGCCGG
CCATTATATAGGGCTGTTCG

Downstream 100 bases:

>100_bases
ACACGGAGGCGGCCTGCTGCGCGCGGCCGCGGAATACGGCATACCCGTCGGCGACTGGCTGGACCTGTCCACCGGCGTCA
ATCCCAACGGCTGGCCGGTG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNSALFLPIALLLDRLLGEPPRWHPLVGFGRLVKAVERAAYPAAPGAEPVWRMRLRGAAAIALLLAPFTLAAWALARLPL
LEIIVPVALLYLAVGARSLAQHAEVVRKALAEGDLQLARERVGWIVSRDTRELDEAGVARATIESVLENGSDAVFAALFW
FLVLGAPGAVLYRLANTLDAMWGYKNERYLHFGWAAARFDDMLNYLPARLTALTYLLLGHAAKGWRCWRTQAPTWYSPNA
GPVMAAGAGALGVSLGGGARYHGQWKERPPLGCGPTPTHEDIGRAVRLVNRGMWLWAALSLAAAILIGAIHA

Sequences:

>Translated_312_residues
MNSALFLPIALLLDRLLGEPPRWHPLVGFGRLVKAVERAAYPAAPGAEPVWRMRLRGAAAIALLLAPFTLAAWALARLPL
LEIIVPVALLYLAVGARSLAQHAEVVRKALAEGDLQLARERVGWIVSRDTRELDEAGVARATIESVLENGSDAVFAALFW
FLVLGAPGAVLYRLANTLDAMWGYKNERYLHFGWAAARFDDMLNYLPARLTALTYLLLGHAAKGWRCWRTQAPTWYSPNA
GPVMAAGAGALGVSLGGGARYHGQWKERPPLGCGPTPTHEDIGRAVRLVNRGMWLWAALSLAAAILIGAIHA
>Mature_312_residues
MNSALFLPIALLLDRLLGEPPRWHPLVGFGRLVKAVERAAYPAAPGAEPVWRMRLRGAAAIALLLAPFTLAAWALARLPL
LEIIVPVALLYLAVGARSLAQHAEVVRKALAEGDLQLARERVGWIVSRDTRELDEAGVARATIESVLENGSDAVFAALFW
FLVLGAPGAVLYRLANTLDAMWGYKNERYLHFGWAAARFDDMLNYLPARLTALTYLLLGHAAKGWRCWRTQAPTWYSPNA
GPVMAAGAGALGVSLGGGARYHGQWKERPPLGCGPTPTHEDIGRAVRLVNRGMWLWAALSLAAAILIGAIHA

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_CHRVO (Q7NXQ1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901245.1
- GeneID:   2551115
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1575
- NMPDR:   fig|243365.1.peg.1575
- HOGENOM:   HBG647131
- OMA:   RWHPLVG
- ProtClustDB:   PRK01209
- BioCyc:   CVIO243365:CV_1575-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 33813; Mature: 33813

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xda7e0e4)-; HASH(0xf80830c)-; HASH(0xf15606c)-; HASH(0xc1497bc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSALFLPIALLLDRLLGEPPRWHPLVGFGRLVKAVERAAYPAAPGAEPVWRMRLRGAAA
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IALLLAPFTLAAWALARLPLLEIIVPVALLYLAVGARSLAQHAEVVRKALAEGDLQLARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGWIVSRDTRELDEAGVARATIESVLENGSDAVFAALFWFLVLGAPGAVLYRLANTLDA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MWGYKNERYLHFGWAAARFDDMLNYLPARLTALTYLLLGHAAKGWRCWRTQAPTWYSPNA
HHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
GPVMAAGAGALGVSLGGGARYHGQWKERPPLGCGPTPTHEDIGRAVRLVNRGMWLWAALS
CCEEECCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
LAAAILIGAIHA
HHHHHHHHHHCH
>Mature Secondary Structure
MNSALFLPIALLLDRLLGEPPRWHPLVGFGRLVKAVERAAYPAAPGAEPVWRMRLRGAAA
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IALLLAPFTLAAWALARLPLLEIIVPVALLYLAVGARSLAQHAEVVRKALAEGDLQLARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGWIVSRDTRELDEAGVARATIESVLENGSDAVFAALFWFLVLGAPGAVLYRLANTLDA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MWGYKNERYLHFGWAAARFDDMLNYLPARLTALTYLLLGHAAKGWRCWRTQAPTWYSPNA
HHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
GPVMAAGAGALGVSLGGGARYHGQWKERPPLGCGPTPTHEDIGRAVRLVNRGMWLWAALS
CCEEECCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
LAAAILIGAIHA
HHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782