Click here to switch to the map view.

The map label for this gene is cbiJ [H]

Identifier: 34497017

GI number: 34497017

Start: 1674390

End: 1675934

Strand: Reverse

Name: cbiJ [H]

Synonym: CV_1562

Alternate gene names: 34497017

Gene position: 1675934-1674390 (Counterclockwise)

Preceding gene: 34497018

Following gene: 34497016

Centisome position: 35.27

GC content: 69.39

Gene sequence:

>1545_bases
ATGACCGGCAAACTCTACCTCGTCTCGGTCGGCCCCGGCCGCGCCGAACTGATCCCCGACCTCGCCCGCCAGGCGCTGGC
GGCGAGCGACGTGATCGTCTCCTACGACCTCTACCTGACCTGGGTGCAGCCGTGGATCGTCGGCAAAGACATCCGCACCC
TGCCGCTGACCCAGGAGCGCGAGCGCGCGCAGCTGGCGCTGGAGGAGGCCCGCGCCGGCAAGACCGTTGCCCTGCTGTCC
AGCGGCGACATCGGCGTCTACGCGATGGCCACGCTGGCCTACGAAGACATGCGCGAGGACGATGTGTTCGACGTGCAGCT
GATCCCCGGCATCACCTCGGCCAATTCCTGCGCGTCGCTGCTCGGCGCGCCGCTGTCGCACGATTTCGCCACGCTGAGCC
TGTCCGACCTGCTGTGCCCGTGGGAGTGGATAGAAACCCGCGCCCGCCACATCGCCCAGGCCGACCTGGCGGTGGTGTTC
TACAACGTGCAGAGCCGCCAACGCCAGGAAGGCGTGTACCGCATCCTGGACATCATGCTGGAGCACAAGAAGCCGGAAAC
GCTGTGCGGCATCGTCCGCAACGCCTACCGCGAAGACCAGGCGGTGGAGATCGTCACGCTGGCCGAGCTGCGGACCCGCC
GCTTCGACATGCTGACCAGCATCGTCATCGGCAACCGCTTCACCCGCCGCAAGCGCGAGTGGATGTTCACCCCGCGCGGC
TACTTCAACTGGTCCAACACCAGCGACACGGTGAAGGCCGACGCGCTGCCCGCCGGCGCGGCCTGGGTGTTTTCCGGCAC
CAGCGACGGCAACGCGCTGTCGCGCGCCATCGCCGAAACCGGCCGTCCGGTGGTGGTGAGCAGCGCCAGCGACTACGGCA
ACGAGCAGGCGCGGCAGGCCGTGCCCGGCGTCGCCACCGTCAGCGGCCGGCTGGGCGTGGAGCGCCGCCGCGAGCTGCTG
TCCGGCAGCCGGGCCAGCGCCATCGTCGACGCCACCCACCCTTACGCCAGCGAGATGTCGCAACAGCTGATGGCGCTGGC
CGAGGAACTGGCCCTGCCCTATCTGCGCTACGAACGGCCAGCCAGCGGCAGCGAACATCCGGTCATCCATTGCGCCGACG
CGGACGCGGCCGCCAGGCTGGCGATGGCTCGCGGCCAGCGCATCTTCCTCGCCACCGGCAGCAAGGAGCTGCACCGCTTC
CTCGCCGCCGACGCCGAGCAGAAACAGCAATGGTTCGTCCGCCTGGCGCCGGACCCGCAGCATTTGCAGCGGGCGCTGGA
CTTAGGTCTTCCGCGCGCGCGCATCTGCGCGATGCAGGGTCCGTTCTCGCAGGCCGCCAACGAGGCGCTGTGGCGCGACT
GGGGCATAGACTGCGTGATCAGCAAGGACGGCGGCGAGGCCGGCGGCTTCGACGCCAAGGCCGCGGCCGCGGCGGCGCTG
GGCATTCCCTTCATCGTCATCGACCGCCCGCAACTGGATTATCCGCAGGCCTTCGGCGACTTCGCCTCGCTGCTGGCGGC
CTTGCCCAAGGGAGAACAAGCATGA

Upstream 100 bases:

>100_bases
CGCACGCAGACAACCCCAGGCAGCCCCTGTTGCCCGAGAAACCATCTGCAAACCAATACCCGACATTGCATAGCCATCAT
CCATGAATACGAATGAACCC

Downstream 100 bases:

>100_bases
GCGCGCCGCGCCTTCCCGTCACCGTGTTGACCGGTTTCCTCGGCGCCGGCAAGACCACGCTGCTGTCCAAGCTGCTGGAG
AACAACAAGCAGCGGCGGCT

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3B C(17)-methyltransferase; Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-8X methylmutase; Cobalt-precorrin isomerase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTGKLYLVSVGPGRAELIPDLARQALAASDVIVSYDLYLTWVQPWIVGKDIRTLPLTQERERAQLALEEARAGKTVALLS
SGDIGVYAMATLAYEDMREDDVFDVQLIPGITSANSCASLLGAPLSHDFATLSLSDLLCPWEWIETRARHIAQADLAVVF
YNVQSRQRQEGVYRILDIMLEHKKPETLCGIVRNAYREDQAVEIVTLAELRTRRFDMLTSIVIGNRFTRRKREWMFTPRG
YFNWSNTSDTVKADALPAGAAWVFSGTSDGNALSRAIAETGRPVVVSSASDYGNEQARQAVPGVATVSGRLGVERRRELL
SGSRASAIVDATHPYASEMSQQLMALAEELALPYLRYERPASGSEHPVIHCADADAAARLAMARGQRIFLATGSKELHRF
LAADAEQKQQWFVRLAPDPQHLQRALDLGLPRARICAMQGPFSQAANEALWRDWGIDCVISKDGGEAGGFDAKAAAAAAL
GIPFIVIDRPQLDYPQAFGDFASLLAALPKGEQA

Sequences:

>Translated_514_residues
MTGKLYLVSVGPGRAELIPDLARQALAASDVIVSYDLYLTWVQPWIVGKDIRTLPLTQERERAQLALEEARAGKTVALLS
SGDIGVYAMATLAYEDMREDDVFDVQLIPGITSANSCASLLGAPLSHDFATLSLSDLLCPWEWIETRARHIAQADLAVVF
YNVQSRQRQEGVYRILDIMLEHKKPETLCGIVRNAYREDQAVEIVTLAELRTRRFDMLTSIVIGNRFTRRKREWMFTPRG
YFNWSNTSDTVKADALPAGAAWVFSGTSDGNALSRAIAETGRPVVVSSASDYGNEQARQAVPGVATVSGRLGVERRRELL
SGSRASAIVDATHPYASEMSQQLMALAEELALPYLRYERPASGSEHPVIHCADADAAARLAMARGQRIFLATGSKELHRF
LAADAEQKQQWFVRLAPDPQHLQRALDLGLPRARICAMQGPFSQAANEALWRDWGIDCVISKDGGEAGGFDAKAAAAAAL
GIPFIVIDRPQLDYPQAFGDFASLLAALPKGEQA
>Mature_513_residues
TGKLYLVSVGPGRAELIPDLARQALAASDVIVSYDLYLTWVQPWIVGKDIRTLPLTQERERAQLALEEARAGKTVALLSS
GDIGVYAMATLAYEDMREDDVFDVQLIPGITSANSCASLLGAPLSHDFATLSLSDLLCPWEWIETRARHIAQADLAVVFY
NVQSRQRQEGVYRILDIMLEHKKPETLCGIVRNAYREDQAVEIVTLAELRTRRFDMLTSIVIGNRFTRRKREWMFTPRGY
FNWSNTSDTVKADALPAGAAWVFSGTSDGNALSRAIAETGRPVVVSSASDYGNEQARQAVPGVATVSGRLGVERRRELLS
GSRASAIVDATHPYASEMSQQLMALAEELALPYLRYERPASGSEHPVIHCADADAAARLAMARGQRIFLATGSKELHRFL
AADAEQKQQWFVRLAPDPQHLQRALDLGLPRARICAMQGPFSQAANEALWRDWGIDCVISKDGGEAGGFDAKAAAAAALG
IPFIVIDRPQLDYPQAFGDFASLLAALPKGEQA

Specific function: Bifunctional enzyme with a methyltransferase domain that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 and an isomerase domain that catalyzes the conversion of cobalt-precorrin-8X to cobyrinic acid; a methyl migration

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CobH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014422
- InterPro:   IPR003722
- InterPro:   IPR006363 [H]

Pfam domain/function: PF02570 CbiC; PF00590 TP_methylase [H]

EC number: 2.1.1.131; 1.3.1.54

Molecular weight: Translated: 56301; Mature: 56169

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKLYLVSVGPGRAELIPDLARQALAASDVIVSYDLYLTWVQPWIVGKDIRTLPLTQER
CCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCEEECCCCEECCCCCHH
ERAQLALEEARAGKTVALLSSGDIGVYAMATLAYEDMREDDVFDVQLIPGITSANSCASL
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
LGAPLSHDFATLSLSDLLCPWEWIETRARHIAQADLAVVFYNVQSRQRQEGVYRILDIML
HCCCCCCCHHEEEHHHHCCCHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHH
EHKKPETLCGIVRNAYREDQAVEIVTLAELRTRRFDMLTSIVIGNRFTRRKREWMFTPRG
HCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCC
YFNWSNTSDTVKADALPAGAAWVFSGTSDGNALSRAIAETGRPVVVSSASDYGNEQARQA
CCCCCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
VPGVATVSGRLGVERRRELLSGSRASAIVDATHPYASEMSQQLMALAEELALPYLRYERP
CCCCEEECCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHCCCC
ASGSEHPVIHCADADAAARLAMARGQRIFLATGSKELHRFLAADAEQKQQWFVRLAPDPQ
CCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCHHHHHHHHHCCHHCCCCEEEEECCCHH
HLQRALDLGLPRARICAMQGPFSQAANEALWRDWGIDCVISKDGGEAGGFDAKAAAAAAL
HHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
GIPFIVIDRPQLDYPQAFGDFASLLAALPKGEQA
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TGKLYLVSVGPGRAELIPDLARQALAASDVIVSYDLYLTWVQPWIVGKDIRTLPLTQER
CCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCEEECCCCEECCCCCHH
ERAQLALEEARAGKTVALLSSGDIGVYAMATLAYEDMREDDVFDVQLIPGITSANSCASL
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
LGAPLSHDFATLSLSDLLCPWEWIETRARHIAQADLAVVFYNVQSRQRQEGVYRILDIML
HCCCCCCCHHEEEHHHHCCCHHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHH
EHKKPETLCGIVRNAYREDQAVEIVTLAELRTRRFDMLTSIVIGNRFTRRKREWMFTPRG
HCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCC
YFNWSNTSDTVKADALPAGAAWVFSGTSDGNALSRAIAETGRPVVVSSASDYGNEQARQA
CCCCCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
VPGVATVSGRLGVERRRELLSGSRASAIVDATHPYASEMSQQLMALAEELALPYLRYERP
CCCCEEECCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHCCCC
ASGSEHPVIHCADADAAARLAMARGQRIFLATGSKELHRFLAADAEQKQQWFVRLAPDPQ
CCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCHHHHHHHHHCCHHCCCCEEEEECCCHH
HLQRALDLGLPRARICAMQGPFSQAANEALWRDWGIDCVISKDGGEAGGFDAKAAAAAAL
HHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
GIPFIVIDRPQLDYPQAFGDFASLLAALPKGEQA
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]