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The map label for this gene is ackA

Identifier: 34496986

GI number: 34496986

Start: 1638380

End: 1639573

Strand: Reverse

Name: ackA

Synonym: CV_1531

Alternate gene names: 34496986

Gene position: 1639573-1638380 (Counterclockwise)

Preceding gene: 34496995

Following gene: 34496985

Centisome position: 34.51

GC content: 65.24

Gene sequence:

>1194_bases
ATGAATTCCAGCATCCTCGTCATCAATTGCGGCAGTTCCTCCCTGAAATTCGCGCTGATCGACAGCGCCAGCCACGAGGC
GGTCATGACCGGCCTGGCCGAGAAGCTGGGCCTGGCCGACGCCTGCATCACCTTCAAGCACAACGGCGACAAGCAAACCG
CCCTGCTGTCCGCACCGGACCACGCCGCCGCCATGCACGCCATCATCGAGAAGCTGCAGCAGGTATCGCTGCTGGACAGC
GTGAAGGCCATCGGCCACCGCGTGGTTCACGGCGGCGAACACTTCAAGCAATCCGCCCTGCTCGACGAAACCGCCATCAA
CGAGATCGAGCGCTGCATCAAGCTGGCCCCGCTGCACAACCCGGCGCACATCCTGGGCATCCGCACCGCGATCAAGGAGT
TCCCGAGCCTGCCGCAAGTGGCCGTGTTCGACACCTCCTTCCACCAGACCATGCCGGAAAAGGCCTACCTGTACGCGGTG
CCGATGAAGCTGTACCGCGAAAACAGCCTGCGCCGCTACGGCATGCACGGCACCAGCTACCGCTTCGTGGCTGAAGAGGC
CGCCAAGATGCTGGGCAAGCCGGCCAATGAAACCAGCCTGGTGATCGCCCACCTGGGCAACGGCGCATCCATCTCCGCCA
TCCGCAACGGCAAGTGCGCCGACACCTCCATGGGCCTGACCCCGCTGGAAGGCCTGGTGATGGGCACCCGCTCCGGCGAC
ATCGACCCCAGCGTGTTCGGCTACCTGGCCACCGAGCGCGGCATGGACATCCAGTCCATCACCAATATGCTGAACAAGGA
ATCGGGCCTCTTGGGCCTGTCCGAGCTGTCCAACGACTGCCGCGAACTGGAAGAAGCCGCCGCCAAGGGCCACGAAGGCG
CCAAGCGCGCGCTGGAAGTGTTCGCCTACCGTCTGGCCAAGTACGTGGCCTCGATGAGCGTGGGCGCCGGCCGCCTGGAC
GCCGTGGTGTTCACCGGCGGCATCGGCGAGAACTCGTCCTTCCTGCGCGCCGAAGTGATCAACCAGCTGGGCTTCCTGGG
CCTGAAGCTGGACGCGGCCGCCAACGAAGCCTGCATCCGCGGCAACGCCGGCCGCATCACCGCCGCCGACTCCGTGCCGG
CGCTGGTGATCAACACCAACGAAGAGCTGATGATCGCCATGGACACCGCCAAGCTGGCCGGCCTGGCCAACTAA

Upstream 100 bases:

>100_bases
GCCCCGGACCGGATATCCGGCAGCAAATTGGCTGCAGGCGCATCGCAGTGGATTTTTTCACCGATTTAGTCCTTTTTTTT
CAAATTACATGTTCAAAGCT

Downstream 100 bases:

>100_bases
GGGGAAGCAGGCCATGCAGACATTCTTCCTCGCCCCCACCGGCTTCAATGCCGGCCTGACCTCGGTCACGCTGGGCGCCA
TCCGTTCGCTGGAGCAGGCC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNSSILVINCGSSSLKFALIDSASHEAVMTGLAEKLGLADACITFKHNGDKQTALLSAPDHAAAMHAIIEKLQQVSLLDS
VKAIGHRVVHGGEHFKQSALLDETAINEIERCIKLAPLHNPAHILGIRTAIKEFPSLPQVAVFDTSFHQTMPEKAYLYAV
PMKLYRENSLRRYGMHGTSYRFVAEEAAKMLGKPANETSLVIAHLGNGASISAIRNGKCADTSMGLTPLEGLVMGTRSGD
IDPSVFGYLATERGMDIQSITNMLNKESGLLGLSELSNDCRELEEAAAKGHEGAKRALEVFAYRLAKYVASMSVGAGRLD
AVVFTGGIGENSSFLRAEVINQLGFLGLKLDAAANEACIRGNAGRITAADSVPALVINTNEELMIAMDTAKLAGLAN

Sequences:

>Translated_397_residues
MNSSILVINCGSSSLKFALIDSASHEAVMTGLAEKLGLADACITFKHNGDKQTALLSAPDHAAAMHAIIEKLQQVSLLDS
VKAIGHRVVHGGEHFKQSALLDETAINEIERCIKLAPLHNPAHILGIRTAIKEFPSLPQVAVFDTSFHQTMPEKAYLYAV
PMKLYRENSLRRYGMHGTSYRFVAEEAAKMLGKPANETSLVIAHLGNGASISAIRNGKCADTSMGLTPLEGLVMGTRSGD
IDPSVFGYLATERGMDIQSITNMLNKESGLLGLSELSNDCRELEEAAAKGHEGAKRALEVFAYRLAKYVASMSVGAGRLD
AVVFTGGIGENSSFLRAEVINQLGFLGLKLDAAANEACIRGNAGRITAADSVPALVINTNEELMIAMDTAKLAGLAN
>Mature_397_residues
MNSSILVINCGSSSLKFALIDSASHEAVMTGLAEKLGLADACITFKHNGDKQTALLSAPDHAAAMHAIIEKLQQVSLLDS
VKAIGHRVVHGGEHFKQSALLDETAINEIERCIKLAPLHNPAHILGIRTAIKEFPSLPQVAVFDTSFHQTMPEKAYLYAV
PMKLYRENSLRRYGMHGTSYRFVAEEAAKMLGKPANETSLVIAHLGNGASISAIRNGKCADTSMGLTPLEGLVMGTRSGD
IDPSVFGYLATERGMDIQSITNMLNKESGLLGLSELSNDCRELEEAAAKGHEGAKRALEVFAYRLAKYVASMSVGAGRLD
AVVFTGGIGENSSFLRAEVINQLGFLGLKLDAAANEACIRGNAGRITAADSVPALVINTNEELMIAMDTAKLAGLAN

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=394, Percent_Identity=53.2994923857868, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI145693184, Length=392, Percent_Identity=46.9387755102041, Blast_Score=352, Evalue=2e-98,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_CHRVO (Q7NXU5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901201.1
- ProteinModelPortal:   Q7NXU5
- GeneID:   2548646
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1531
- NMPDR:   fig|243365.1.peg.1531
- HOGENOM:   HBG285863
- OMA:   KENHIRR
- PhylomeDB:   Q7NXU5
- BioCyc:   CVIO243365:CV_1531-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42278; Mature: 42278

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSILVINCGSSSLKFALIDSASHEAVMTGLAEKLGLADACITFKHNGDKQTALLSAPD
CCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCC
HAAAMHAIIEKLQQVSLLDSVKAIGHRVVHGGEHFKQSALLDETAINEIERCIKLAPLHN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAHILGIRTAIKEFPSLPQVAVFDTSFHQTMPEKAYLYAVPMKLYRENSLRRYGMHGTSY
CHHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCEEEEEEHHHHHHHCCHHHHCCCCCCC
RFVAEEAAKMLGKPANETSLVIAHLGNGASISAIRNGKCADTSMGLTPLEGLVMGTRSGD
HHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCC
IDPSVFGYLATERGMDIQSITNMLNKESGLLGLSELSNDCRELEEAAAKGHEGAKRALEV
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FAYRLAKYVASMSVGAGRLDAVVFTGGIGENSSFLRAEVINQLGFLGLKLDAAANEACIR
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCEEE
GNAGRITAADSVPALVINTNEELMIAMDTAKLAGLAN
CCCCCEEECCCCCEEEEECCCCEEEEEECHHHCCCCC
>Mature Secondary Structure
MNSSILVINCGSSSLKFALIDSASHEAVMTGLAEKLGLADACITFKHNGDKQTALLSAPD
CCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCC
HAAAMHAIIEKLQQVSLLDSVKAIGHRVVHGGEHFKQSALLDETAINEIERCIKLAPLHN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAHILGIRTAIKEFPSLPQVAVFDTSFHQTMPEKAYLYAVPMKLYRENSLRRYGMHGTSY
CHHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCEEEEEEHHHHHHHCCHHHHCCCCCCC
RFVAEEAAKMLGKPANETSLVIAHLGNGASISAIRNGKCADTSMGLTPLEGLVMGTRSGD
HHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCC
IDPSVFGYLATERGMDIQSITNMLNKESGLLGLSELSNDCRELEEAAAKGHEGAKRALEV
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FAYRLAKYVASMSVGAGRLDAVVFTGGIGENSSFLRAEVINQLGFLGLKLDAAANEACIR
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCEEE
GNAGRITAADSVPALVINTNEELMIAMDTAKLAGLAN
CCCCCEEECCCCCEEEEECCCCEEEEEECHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782