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The map label for this gene is pnp

Identifier: 34496921

GI number: 34496921

Start: 1555925

End: 1558093

Strand: Direct

Name: pnp

Synonym: CV_1466

Alternate gene names: 34496921

Gene position: 1555925-1558093 (Clockwise)

Preceding gene: 34496920

Following gene: 34496922

Centisome position: 32.75

GC content: 63.76

Gene sequence:

>2169_bases
GTGAACCAAGATAGGGAAACCCTCGTGTTCAACAAGATCACCAAAACCTTCCAGTTCGGCCGCCAGACCGTCACCTTGGA
AACCGGCGAAATCGCTCGCCAGGCATCCGGTTCCGTAGTCGTTTCCGTTGACGACACCGTCGTCATGGTCAGCGTGGTGG
GCGGCAAAAACGTCAAGCCGGGCCAGGACTTCTTCCCGCTGACCGTCGACTACCTCGAGCGTACCTACGCCGCCGGCAAG
ATCCCGGGCGGCTTCTTCAAGCGCGAAGGCAAGCAGTCCGAGAAGGAAGTGCTGACCAGCCGCCTGATCGACCGTCCGAT
CCGCCCGCTGTTCCCGGAAGGCTTCTACCATGACGTGCAGATCGTCGCCACCGTGTTGTCGCTGAATCCGGAAGTGGATT
CCGACATCCCGGCGATGATCGGCGCTTCCGCCGCGCTGGCCATTTCCGGCCTGCCGTTCGCCGGCCCGATCGGCGCCGCC
CGCGTCGGCTACGCCAACGGCGAATACATCCTGAACCCGACCAAGACCGAGCTGCAAACCTCGCAGATGGATCTGGTGGT
CGCCGGCACCTCGCGCGCCGTGCTGATGGTGGAATCTGAAGCCAAGGAACTGCCGGAAGCCGTGATGCTGGGCGCTGTGG
TGTTCGGCCACGAACAGATGCAGGCTGCGATCAAGGCCATCAACGAACTGGCCGACGAAGTGAATCCGGTGATGTTCGAC
TGGGCCGCTCCGGCCAAGGACGAAGCGCTGATCGCCCAGATCCGCGGCATCGCCGGCGAGAAGCTGGCCGAAGCCTTCCG
CCTGCGCCAGAAGCAAGCCCGCACCGTCGCCATCAACGAAGCTTGGGACGCGGTCAAGGCCGGCCTGATCAACGAAGAAA
CCGACACCCTGAAGGCCAACGAGATCAAGGGCATCTTCAAGAGCCTGGAAGCTGAAATCGTCCGCGGCCAGATCCTGGCT
GGCGAAGCGCGCATCGACGGCCGCGACACCCGCACCGTGCGTCCGATCAGCATCCGCAACAACGTGCTGCCGCGCACCCA
CGGCTCCGCGCTGTTCACCCGCGGCGAAACCCAGGCGCTGGTCGTGGCCACGCTGGGCACCAAGCAGGACGAGCAGATCA
TCGACGCGCTGGCCGGCGAGTACACCGAGCGCTTCATGCTGCACTACAACTTCCCGCCGTACTCCACCGGCGAAGCCGGC
CGCATGGGCCCGCCGAAGCGCCGCGAAATCGGCCACGGCCGCCTGGCCAAGCGCGCGCTGGTGGCCGTGCTGCCGCCGGA
AGACGAGTTCGGCTACTCGATGCGCGTGGTGTCCGAGATCACCGAATCCAACGGCTCCTCGTCGATGGCTTCCGTGTGCG
GCGGCTGCCTGTCGCTGCTGTCGGCCGGCGTGCCGCTGAAGGCGCACGTGGCCGGCATCGCCATGGGCCTGATCCTGGAA
GGCAACCGCTTCGCCGTGCTGACCGACATCCTGGGCGATGAAGATCACCTGGGCGACATGGACTTCAAGGTGGCCGGCAC
CGCCGCAGGCGTGACCGCGCTGCAGATGGACATCAAGATCCAGGGCATCACCAAGGAAATCATGCAGGTTGCGCTGGAGC
AGGCCAAGGAAGGCCGTCTGCACATCCTGGGCCTGATGAAGGAAGCCGTGGACGGCCCGCAAGAGCTGTCCGCCCACGCT
CCGCGCCTGTACGTGATGAAGATCAACCCGGAGAAGATCCGCGAAGTGATCGGCAAGGGCGGCGAGACCATCCGCTCCAT
CACCAAGGATACCGGCTGCGAGATCAACATCGAAGAAGACGGCACCATCACCATCGCCTCGGTAAGCAGCGAGGGCGCGG
AAGCGGCCAAGAAGCGCATCGAAGAGATCACTGTCGAAGTCGAAGTGGGCAAGGTGTACGAAGGCACCGTGGTCAAGATC
CTCGACAACAACGTCGGCGCCATCGTTTCCATCCTGCCGGGCAAGGACGGTCTGGTCCACATCTCGCAGATCGCCAACGA
GCGCATCAAGAATGTGTCCGACTACCTGAAGGAAGGCCAGGTTGTGAAGGTCAAGGCCATCGAAATGGATGACCGCGGCC
GCATCCGCCTGTCCATCAAGGCGCTGCTGGAAGAAGAAGCGAAAACCGCCCAAGCGACCGCTGACGCCTTCGGCCTGAAG
ACCCAGTAA

Upstream 100 bases:

>100_bases
TGTCGTAACAACGGGGGGAGCCTCTACAAATTCCGATCTTTCGTCCAGGTCCGAATTTTTAGGGGTTCCCGTTGTTTTAT
CCGCTCGCGCATTTTCATCA

Downstream 100 bases:

>100_bases
GTCCGGTTGATAGCCGTTCCTGCCTGGAGCGGACGCCAGCCAGAACCCCCTGCCATGGCAGGGGGTTTTGTTTTAAATCG
CGAGGCTGTTTAATTGACTG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MNQDRETLVFNKITKTFQFGRQTVTLETGEIARQASGSVVVSVDDTVVMVSVVGGKNVKPGQDFFPLTVDYLERTYAAGK
IPGGFFKREGKQSEKEVLTSRLIDRPIRPLFPEGFYHDVQIVATVLSLNPEVDSDIPAMIGASAALAISGLPFAGPIGAA
RVGYANGEYILNPTKTELQTSQMDLVVAGTSRAVLMVESEAKELPEAVMLGAVVFGHEQMQAAIKAINELADEVNPVMFD
WAAPAKDEALIAQIRGIAGEKLAEAFRLRQKQARTVAINEAWDAVKAGLINEETDTLKANEIKGIFKSLEAEIVRGQILA
GEARIDGRDTRTVRPISIRNNVLPRTHGSALFTRGETQALVVATLGTKQDEQIIDALAGEYTERFMLHYNFPPYSTGEAG
RMGPPKRREIGHGRLAKRALVAVLPPEDEFGYSMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILE
GNRFAVLTDILGDEDHLGDMDFKVAGTAAGVTALQMDIKIQGITKEIMQVALEQAKEGRLHILGLMKEAVDGPQELSAHA
PRLYVMKINPEKIREVIGKGGETIRSITKDTGCEINIEEDGTITIASVSSEGAEAAKKRIEEITVEVEVGKVYEGTVVKI
LDNNVGAIVSILPGKDGLVHISQIANERIKNVSDYLKEGQVVKVKAIEMDDRGRIRLSIKALLEEEAKTAQATADAFGLK
TQ

Sequences:

>Translated_722_residues
MNQDRETLVFNKITKTFQFGRQTVTLETGEIARQASGSVVVSVDDTVVMVSVVGGKNVKPGQDFFPLTVDYLERTYAAGK
IPGGFFKREGKQSEKEVLTSRLIDRPIRPLFPEGFYHDVQIVATVLSLNPEVDSDIPAMIGASAALAISGLPFAGPIGAA
RVGYANGEYILNPTKTELQTSQMDLVVAGTSRAVLMVESEAKELPEAVMLGAVVFGHEQMQAAIKAINELADEVNPVMFD
WAAPAKDEALIAQIRGIAGEKLAEAFRLRQKQARTVAINEAWDAVKAGLINEETDTLKANEIKGIFKSLEAEIVRGQILA
GEARIDGRDTRTVRPISIRNNVLPRTHGSALFTRGETQALVVATLGTKQDEQIIDALAGEYTERFMLHYNFPPYSTGEAG
RMGPPKRREIGHGRLAKRALVAVLPPEDEFGYSMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILE
GNRFAVLTDILGDEDHLGDMDFKVAGTAAGVTALQMDIKIQGITKEIMQVALEQAKEGRLHILGLMKEAVDGPQELSAHA
PRLYVMKINPEKIREVIGKGGETIRSITKDTGCEINIEEDGTITIASVSSEGAEAAKKRIEEITVEVEVGKVYEGTVVKI
LDNNVGAIVSILPGKDGLVHISQIANERIKNVSDYLKEGQVVKVKAIEMDDRGRIRLSIKALLEEEAKTAQATADAFGLK
TQ
>Mature_722_residues
MNQDRETLVFNKITKTFQFGRQTVTLETGEIARQASGSVVVSVDDTVVMVSVVGGKNVKPGQDFFPLTVDYLERTYAAGK
IPGGFFKREGKQSEKEVLTSRLIDRPIRPLFPEGFYHDVQIVATVLSLNPEVDSDIPAMIGASAALAISGLPFAGPIGAA
RVGYANGEYILNPTKTELQTSQMDLVVAGTSRAVLMVESEAKELPEAVMLGAVVFGHEQMQAAIKAINELADEVNPVMFD
WAAPAKDEALIAQIRGIAGEKLAEAFRLRQKQARTVAINEAWDAVKAGLINEETDTLKANEIKGIFKSLEAEIVRGQILA
GEARIDGRDTRTVRPISIRNNVLPRTHGSALFTRGETQALVVATLGTKQDEQIIDALAGEYTERFMLHYNFPPYSTGEAG
RMGPPKRREIGHGRLAKRALVAVLPPEDEFGYSMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILE
GNRFAVLTDILGDEDHLGDMDFKVAGTAAGVTALQMDIKIQGITKEIMQVALEQAKEGRLHILGLMKEAVDGPQELSAHA
PRLYVMKINPEKIREVIGKGGETIRSITKDTGCEINIEEDGTITIASVSSEGAEAAKKRIEEITVEVEVGKVYEGTVVKI
LDNNVGAIVSILPGKDGLVHISQIANERIKNVSDYLKEGQVVKVKAIEMDDRGRIRLSIKALLEEEAKTAQATADAFGLK
TQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=36.6058906030856, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI145693187, Length=698, Percent_Identity=61.1747851002865, Blast_Score=870, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=719, Percent_Identity=32.5452016689847, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI281362905, Length=716, Percent_Identity=36.8715083798883, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651641, Length=716, Percent_Identity=36.8715083798883, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651643, Length=716, Percent_Identity=36.8715083798883, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.3774733637747, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHRVO (Q7NY10)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901136.1
- HSSP:   P05055
- ProteinModelPortal:   Q7NY10
- GeneID:   2550306
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1466
- NMPDR:   fig|243365.1.peg.1466
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q7NY10
- ProtClustDB:   PRK11824
- BioCyc:   CVIO243365:CV_1466-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77891; Mature: 77891

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDRETLVFNKITKTFQFGRQTVTLETGEIARQASGSVVVSVDDTVVMVSVVGGKNVKP
CCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCC
GQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEVLTSRLIDRPIRPLFPEGFYHDVQ
CCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
IVATVLSLNPEVDSDIPAMIGASAALAISGLPFAGPIGAARVGYANGEYILNPTKTELQT
HHHHHHHCCCCCCCCCHHHHCCCHHHEECCCCCCCCCCCCEEECCCCCEEECCCHHHHHH
SQMDLVVAGTSRAVLMVESEAKELPEAVMLGAVVFGHEQMQAAIKAINELADEVNPVMFD
CCEEEEEECCCCEEEEECCHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEE
WAAPAKDEALIAQIRGIAGEKLAEAFRLRQKQARTVAINEAWDAVKAGLINEETDTLKAN
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCCCHH
EIKGIFKSLEAEIVRGQILAGEARIDGRDTRTVRPISIRNNVLPRTHGSALFTRGETQAL
HHHHHHHHHHHHHHHCEEEECCEEECCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEE
VVATLGTKQDEQIIDALAGEYTERFMLHYNFPPYSTGEAGRMGPPKRREIGHGRLAKRAL
EEEECCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHEE
VAVLPPEDEFGYSMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILE
EEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEE
GNRFAVLTDILGDEDHLGDMDFKVAGTAAGVTALQMDIKIQGITKEIMQVALEQAKEGRL
CCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCCCE
HILGLMKEAVDGPQELSAHAPRLYVMKINPEKIREVIGKGGETIRSITKDTGCEINIEED
EEEHHHHHHCCCHHHHHCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCEEEECCC
GTITIASVSSEGAEAAKKRIEEITVEVEVGKVYEGTVVKILDNNVGAIVSILPGKDGLVH
CEEEEEECCCCCHHHHHHHHHHEEEEEEECCEECCEEEEEEECCCCEEEEEECCCCCCEE
ISQIANERIKNVSDYLKEGQVVKVKAIEMDDRGRIRLSIKALLEEEAKTAQATADAFGLK
HHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
TQ
CC
>Mature Secondary Structure
MNQDRETLVFNKITKTFQFGRQTVTLETGEIARQASGSVVVSVDDTVVMVSVVGGKNVKP
CCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCC
GQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEVLTSRLIDRPIRPLFPEGFYHDVQ
CCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
IVATVLSLNPEVDSDIPAMIGASAALAISGLPFAGPIGAARVGYANGEYILNPTKTELQT
HHHHHHHCCCCCCCCCHHHHCCCHHHEECCCCCCCCCCCCEEECCCCCEEECCCHHHHHH
SQMDLVVAGTSRAVLMVESEAKELPEAVMLGAVVFGHEQMQAAIKAINELADEVNPVMFD
CCEEEEEECCCCEEEEECCHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEE
WAAPAKDEALIAQIRGIAGEKLAEAFRLRQKQARTVAINEAWDAVKAGLINEETDTLKAN
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCCCHH
EIKGIFKSLEAEIVRGQILAGEARIDGRDTRTVRPISIRNNVLPRTHGSALFTRGETQAL
HHHHHHHHHHHHHHHCEEEECCEEECCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEE
VVATLGTKQDEQIIDALAGEYTERFMLHYNFPPYSTGEAGRMGPPKRREIGHGRLAKRAL
EEEECCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHEE
VAVLPPEDEFGYSMRVVSEITESNGSSSMASVCGGCLSLLSAGVPLKAHVAGIAMGLILE
EEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEE
GNRFAVLTDILGDEDHLGDMDFKVAGTAAGVTALQMDIKIQGITKEIMQVALEQAKEGRL
CCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCCCE
HILGLMKEAVDGPQELSAHAPRLYVMKINPEKIREVIGKGGETIRSITKDTGCEINIEED
EEEHHHHHHCCCHHHHHCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCEEEECCC
GTITIASVSSEGAEAAKKRIEEITVEVEVGKVYEGTVVKILDNNVGAIVSILPGKDGLVH
CEEEEEECCCCCHHHHHHHHHHEEEEEEECCEECCEEEEEEECCCCEEEEEECCCCCCEE
ISQIANERIKNVSDYLKEGQVVKVKAIEMDDRGRIRLSIKALLEEEAKTAQATADAFGLK
HHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
TQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782