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The map label for this gene is truB

Identifier: 34496919

GI number: 34496919

Start: 1554472

End: 1555392

Strand: Direct

Name: truB

Synonym: CV_1464

Alternate gene names: 34496919

Gene position: 1554472-1555392 (Clockwise)

Preceding gene: 34496918

Following gene: 34496920

Centisome position: 32.72

GC content: 66.12

Gene sequence:

>921_bases
ATGAGCAAGCCGCGCAGCAACCGCCGCCACATCGATGGCGTGCTGCTGATCGACAAGCCTTACGACATTTCCAGCAACAA
TGCGCTGCAGAAGGCGCGCTGGCTGCTCAATGCCGCCAAGGCCGGCCATACCGGCGTGCTGGACCCGCTGGCGACCGGCC
TGCTGCCGGTGTGCCTGGGGGAAGCCACCAAGTTCTCCTCGTATCTGCTGGACGCGGACAAGGGGTACCGCGCCACGGTC
AGGTTCGGCGTAGTGACGACGACCGGCGACGTGGAGGGCGAAGTGGTGTCCGAGCGGCCGGTCGAATTCGGCCGCGAGCA
GCTGGAAGCCGCGCTGGCGCGCTTTCGCGGCGAAATCAGCCAGGTGCCGCCGATGTATTCGGCGCTGAAGCATCAGGGCA
AGCCGCTGTACGAATACGCGCGCGCCGGCATCGAGGTGCCGCGCGAGGCGCGCCAGGTCACCATCCGCAAGCTGGAGCTG
CTGTCGTTCGACGGCGTGTCGGCCGAGATCGACGTGCTGTGCACCAAGGGGACGTATATCCGCACGCTGGGCTGCGACAT
CGGCGAGGCGCTGGGCTGCGGCGCGCATCTGACCGCGCTGCGCCGCACCGCCACCGGCGGTTTCAGCCTGGACGAATCGC
ACCGGCTGGCCGATCTGGAATCGCTGGACATGCCGGCGCGCGAAGCCTTGTTGATGCCGGCGGATGTGCTGGTGATGCAT
TTTCCGCAGCTGGAGCTGGCGGATGAGGAGATCGGCAAGTTTCTTCACGGCCAGCCCGTGCGTTTTGAACAAAAGTGTGA
GAAAATGCAGCGTTTCCGGGTTTACCAACAGTCTTCCCGGAGATTCGTGGGGCTGGGCGAGGCTCGCGGCGATGGCCGCC
TGCATCCGATCCGGCTCCTGGCGAACCAGGCCCAGGCCTGA

Upstream 100 bases:

>100_bases
GCATGCACCTGACCAGCCTGATCAATCAGGTGGCGCGCGAGGATGCCGAGAAGTTCGGCGAGGCGGCCGCTGGCGAAGCC
GAAGGCGAGGGCAAGGCCGA

Downstream 100 bases:

>100_bases
TTCGCCACTTACCGGAGTGTGGAATGGGTTGGGTTTTCCGGCTCGCCGCACTTGTCGGGTTTTCAAACCCCTATTGAAAC
GGAGCAATCCAAATGGCAAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSKPRSNRRHIDGVLLIDKPYDISSNNALQKARWLLNAAKAGHTGVLDPLATGLLPVCLGEATKFSSYLLDADKGYRATV
RFGVVTTTGDVEGEVVSERPVEFGREQLEAALARFRGEISQVPPMYSALKHQGKPLYEYARAGIEVPREARQVTIRKLEL
LSFDGVSAEIDVLCTKGTYIRTLGCDIGEALGCGAHLTALRRTATGGFSLDESHRLADLESLDMPAREALLMPADVLVMH
FPQLELADEEIGKFLHGQPVRFEQKCEKMQRFRVYQQSSRRFVGLGEARGDGRLHPIRLLANQAQA

Sequences:

>Translated_306_residues
MSKPRSNRRHIDGVLLIDKPYDISSNNALQKARWLLNAAKAGHTGVLDPLATGLLPVCLGEATKFSSYLLDADKGYRATV
RFGVVTTTGDVEGEVVSERPVEFGREQLEAALARFRGEISQVPPMYSALKHQGKPLYEYARAGIEVPREARQVTIRKLEL
LSFDGVSAEIDVLCTKGTYIRTLGCDIGEALGCGAHLTALRRTATGGFSLDESHRLADLESLDMPAREALLMPADVLVMH
FPQLELADEEIGKFLHGQPVRFEQKCEKMQRFRVYQQSSRRFVGLGEARGDGRLHPIRLLANQAQA
>Mature_305_residues
SKPRSNRRHIDGVLLIDKPYDISSNNALQKARWLLNAAKAGHTGVLDPLATGLLPVCLGEATKFSSYLLDADKGYRATVR
FGVVTTTGDVEGEVVSERPVEFGREQLEAALARFRGEISQVPPMYSALKHQGKPLYEYARAGIEVPREARQVTIRKLELL
SFDGVSAEIDVLCTKGTYIRTLGCDIGEALGCGAHLTALRRTATGGFSLDESHRLADLESLDMPAREALLMPADVLVMHF
PQLELADEEIGKFLHGQPVRFEQKCEKMQRFRVYQQSSRRFVGLGEARGDGRLHPIRLLANQAQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=186, Percent_Identity=38.7096774193548, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI4503337, Length=277, Percent_Identity=28.5198555956679, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI215599015, Length=277, Percent_Identity=28.5198555956679, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=50.3205128205128, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17553978, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=131, Percent_Identity=45.8015267175573, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323204, Length=257, Percent_Identity=27.2373540856031, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CHRVO (Q7MBF7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901134.1
- ProteinModelPortal:   Q7MBF7
- SMR:   Q7MBF7
- GeneID:   2550352
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1464
- NMPDR:   fig|243365.1.peg.1464
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q7MBF7
- ProtClustDB:   PRK05033
- BioCyc:   CVIO243365:CV_1464-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33828; Mature: 33697

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPRSNRRHIDGVLLIDKPYDISSNNALQKARWLLNAAKAGHTGVLDPLATGLLPVCLG
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
EATKFSSYLLDADKGYRATVRFGVVTTTGDVEGEVVSERPVEFGREQLEAALARFRGEIS
CHHHHHHHHEECCCCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QVPPMYSALKHQGKPLYEYARAGIEVPREARQVTIRKLELLSFDGVSAEIDVLCTKGTYI
CCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEE
RTLGCDIGEALGCGAHLTALRRTATGGFSLDESHRLADLESLDMPAREALLMPADVLVMH
EEECCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHCCHHHHHHC
FPQLELADEEIGKFLHGQPVRFEQKCEKMQRFRVYQQSSRRFVGLGEARGDGRLHPIRLL
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
ANQAQA
HHHCCC
>Mature Secondary Structure 
SKPRSNRRHIDGVLLIDKPYDISSNNALQKARWLLNAAKAGHTGVLDPLATGLLPVCLG
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
EATKFSSYLLDADKGYRATVRFGVVTTTGDVEGEVVSERPVEFGREQLEAALARFRGEIS
CHHHHHHHHEECCCCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QVPPMYSALKHQGKPLYEYARAGIEVPREARQVTIRKLELLSFDGVSAEIDVLCTKGTYI
CCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEE
RTLGCDIGEALGCGAHLTALRRTATGGFSLDESHRLADLESLDMPAREALLMPADVLVMH
EEECCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHCCHHHHHHC
FPQLELADEEIGKFLHGQPVRFEQKCEKMQRFRVYQQSSRRFVGLGEARGDGRLHPIRLL
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
ANQAQA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782