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The map label for this gene is mscL

Identifier: 34496815

GI number: 34496815

Start: 1431782

End: 1432243

Strand: Reverse

Name: mscL

Synonym: CV_1360

Alternate gene names: 34496815

Gene position: 1432243-1431782 (Counterclockwise)

Preceding gene: 34496816

Following gene: 34496814

Centisome position: 30.15

GC content: 64.29

Gene sequence:

>462_bases
ATGTCCGTTCTGAAGGAATTCAAGGAGTTCGCCGTCAAAGGCAATGTGATCGACCTCGCGGTCGGCGTCGTGATCGGCGG
CGCCTTCGGCTCCATCGTCAAATCGCTGGTCGACGACGTGATCATGCCGCCCATCGGCCTGCTGATCGGCAACGTGGACT
TCTCCAACCTGTTCTTCGTGCTGAAGGACGGCGCCAAGCAGGCCGGCCCCTACGTCTCCGTGGCCGCCGCCAAGCAGGCC
GGCGCCACCACGCTGAACCTGGGCCTGTTCATCAACGCCCTGGTCAGCTTCACCATCGTCGCCTTCGCCATCTTCATGCT
GGTCAAGGCCATCAACCGGCTGAAGCGCGAAGAAGCCGCGCCGGCGCCCGCCGCCCCCGCCACCAAGGAATGCCGCTATT
GCCTGTCCGCCATTCCCGAAAAGGCGACCCGCTGCCCCTGCTGCACCTCCCAGCTGGATTAG

Upstream 100 bases:

>100_bases
CGCTGCCCGGCCGCCGCCACGCGCAAACGCAAGCCACCTAGCTTTGCCAAAAACATAACAAAACAGTTACAATAATCACT
ATCAAATAAAAGGAAAAACC

Downstream 100 bases:

>100_bases
GCGCGCGCCCTTTGCGCTTGCCCGCGTCCGGCTATTCCCCTACAATCGCTTCCGTTCTGGGGGAGTAGCCTCGGCGCGCG
ACAGCGCCGGCACCGGTCAA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSVLKEFKEFAVKGNVIDLAVGVVIGGAFGSIVKSLVDDVIMPPIGLLIGNVDFSNLFFVLKDGAKQAGPYVSVAAAKQA
GATTLNLGLFINALVSFTIVAFAIFMLVKAINRLKREEAAPAPAAPATKECRYCLSAIPEKATRCPCCTSQLD

Sequences:

>Translated_153_residues
MSVLKEFKEFAVKGNVIDLAVGVVIGGAFGSIVKSLVDDVIMPPIGLLIGNVDFSNLFFVLKDGAKQAGPYVSVAAAKQA
GATTLNLGLFINALVSFTIVAFAIFMLVKAINRLKREEAAPAPAAPATKECRYCLSAIPEKATRCPCCTSQLD
>Mature_152_residues
SVLKEFKEFAVKGNVIDLAVGVVIGGAFGSIVKSLVDDVIMPPIGLLIGNVDFSNLFFVLKDGAKQAGPYVSVAAAKQAG
ATTLNLGLFINALVSFTIVAFAIFMLVKAINRLKREEAAPAPAAPATKECRYCLSAIPEKATRCPCCTSQLD

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=130, Percent_Identity=50.7692307692308, Blast_Score=125, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CHRVO (Q7NYB4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901030.1
- ProteinModelPortal:   Q7NYB4
- SMR:   Q7NYB4
- GeneID:   2551048
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1360
- NMPDR:   fig|243365.1.peg.1360
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q7NYB4
- ProtClustDB:   PRK00567
- BioCyc:   CVIO243365:CV_1360-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16094; Mature: 15963

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd0b5aac)-; HASH(0xf28740c)-;

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLKEFKEFAVKGNVIDLAVGVVIGGAFGSIVKSLVDDVIMPPIGLLIGNVDFSNLFFV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHH
LKDGAKQAGPYVSVAAAKQAGATTLNLGLFINALVSFTIVAFAIFMLVKAINRLKREEAA
HHCCCHHCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAPAAPATKECRYCLSAIPEKATRCPCCTSQLD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure 
SVLKEFKEFAVKGNVIDLAVGVVIGGAFGSIVKSLVDDVIMPPIGLLIGNVDFSNLFFV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHH
LKDGAKQAGPYVSVAAAKQAGATTLNLGLFINALVSFTIVAFAIFMLVKAINRLKREEAA
HHCCCHHCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAPAAPATKECRYCLSAIPEKATRCPCCTSQLD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782