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The map label for this gene is 34496657

Identifier: 34496657

GI number: 34496657

Start: 1265663

End: 1266106

Strand: Reverse

Name: 34496657

Synonym: CV_1202

Alternate gene names: NA

Gene position: 1266106-1265663 (Counterclockwise)

Preceding gene: 34496658

Following gene: 34496656

Centisome position: 26.65

GC content: 66.67

Gene sequence:

>444_bases
ATGAAAACAAGATTGCTGTTACTGCTGCTGTCGGCGCTGAGCCTCCCCGCGCTGGCCGCGCCGTCCTGTCGCGACGTCGC
CGCCAAGGTCAATCAGCAAGCCTCGCGCCAGCTGGACGCGGCCGAACTCGGCGACGTGCTGCAAAGCCTGGGCCGCGACG
GCCGGCTGCCAGGCAAGTTCGTCACCAAGCGCCAGGCCCAGGCCGCCGGCTGGAAGCCGGGCCGCAACCTGTGGAGCGTG
GCCGGCCTGCAAGGCAAGTCCATGGGCGGAGACCGCTTCGGCAACTACGAAAAACGTCTGCCGCCCGGCCAATGGCAGGA
GGCGGATTTGAGCTATAAAGGCGGCAAGCGCGGCGCCAAGCGCCTGCTGTTCAGCCGCCAGGGCCAGCGCTTCGTCACCG
TGGATCACTACAACAGCTTCATCGAGGTCCCGCCATGCCAGTAA

Upstream 100 bases:

>100_bases
CATCCCTATCCGGTGGAGTCGCAGCCGCTGGACTGAACGCTTCTCCCGCCTCCGCCGCCCGGCTAGAATGGAGCGGCACC
CTTTGCCTCCAGGTTTCATC

Downstream 100 bases:

>100_bases
AAGTGTGCGAGCTGCGCCATATCCGCAGCCTGGATCAACTGTACGACGAGCTGCAGCGCCAGCTGCCGCTGCCCGGCCAT
TTCGGCCGCAATCTGGACGC

Product: ribonuclease

Products: NA

Alternate protein names: Barnase; RNase Ba [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKTRLLLLLLSALSLPALAAPSCRDVAAKVNQQASRQLDAAELGDVLQSLGRDGRLPGKFVTKRQAQAAGWKPGRNLWSV
AGLQGKSMGGDRFGNYEKRLPPGQWQEADLSYKGGKRGAKRLLFSRQGQRFVTVDHYNSFIEVPPCQ

Sequences:

>Translated_147_residues
MKTRLLLLLLSALSLPALAAPSCRDVAAKVNQQASRQLDAAELGDVLQSLGRDGRLPGKFVTKRQAQAAGWKPGRNLWSV
AGLQGKSMGGDRFGNYEKRLPPGQWQEADLSYKGGKRGAKRLLFSRQGQRFVTVDHYNSFIEVPPCQ
>Mature_147_residues
MKTRLLLLLLSALSLPALAAPSCRDVAAKVNQQASRQLDAAELGDVLQSLGRDGRLPGKFVTKRQAQAAGWKPGRNLWSV
AGLQGKSMGGDRFGNYEKRLPPGQWQEADLSYKGGKRGAKRLLFSRQGQRFVTVDHYNSFIEVPPCQ

Specific function: Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonuclease N1/T1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001887
- InterPro:   IPR000026
- InterPro:   IPR016191 [H]

Pfam domain/function: PF00545 Ribonuclease [H]

EC number: NA

Molecular weight: Translated: 16136; Mature: 16136

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLLLLLLSALSLPALAAPSCRDVAAKVNQQASRQLDAAELGDVLQSLGRDGRLPGKF
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHH
VTKRQAQAAGWKPGRNLWSVAGLQGKSMGGDRFGNYEKRLPPGQWQEADLSYKGGKRGAK
HHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RLLFSRQGQRFVTVDHYNSFIEVPPCQ
HHHHHCCCCEEEEECCCCCEECCCCCC
>Mature Secondary Structure
MKTRLLLLLLSALSLPALAAPSCRDVAAKVNQQASRQLDAAELGDVLQSLGRDGRLPGKF
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHH
VTKRQAQAAGWKPGRNLWSVAGLQGKSMGGDRFGNYEKRLPPGQWQEADLSYKGGKRGAK
HHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RLLFSRQGQRFVTVDHYNSFIEVPPCQ
HHHHHCCCCEEEEECCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3007290; 3050134; 4553460; 1888730; 1569554; 9708913; 2023257; 11914482; 2696173 [H]