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The map label for this gene is rpfG [H]

Identifier: 34496644

GI number: 34496644

Start: 1253264

End: 1254283

Strand: Reverse

Name: rpfG [H]

Synonym: CV_1189

Alternate gene names: 34496644

Gene position: 1254283-1253264 (Counterclockwise)

Preceding gene: 34496645

Following gene: 34496641

Centisome position: 26.4

GC content: 64.9

Gene sequence:

>1020_bases
ATGCAGCTCTCCCCTCCCTTGCTCGTCGTCGACGACGAACCGCAAAACCTCGCGGCGATGCGCCAGGTGCTGGCCCCGCA
ATATCCGTTGGTGTTCGCGCGCAACGGCGCCGAGGCGCTGGCCGCCACCGCCAAGCATAATCCCTCGCTGGTGCTGCTGG
ACATCGAGATGCCGGATATGGACGGTTACGAAGTGTGCCGCCGCATCAAGAAGGATCCGCAGTCCTCGTCGACGCCGGTG
ATCTTCGTCACCTCGCTGGGGGAAATCGGCAACGAGGAAGCCGGCTTCGAAGCCGGCGCGGTCGACTACCTGATCAAACC
GGTTTCGCCGTCCATCGTGAAGGCCAGGGTGCGCACCCATTTGTCACTGGTGCAGGCCACCCAACTGGAAAAGAGCCACC
GCGACGCGATCTACATGCTGGGCCAGGCCGGTCACTTCAATGACACCGACACCGGGGTCCACATCTGGCGGATGGCCGCC
TACTCCGCGATGCTGGCCTCGACCAGCGGCTGGGACGCGGCCGCCTGCCACCTGCTGGAACTGGCCGCGCCGATGCACGA
CACCGGCAAACTGGGCATACCGGACGCCATCCTGCGCAAGCCCGGCGCGTTGACCCCGGACGAATGGGAGGTGATGAAGA
CCCATACCCGGATCGGTCACGACATCCTGATCCGCAGCGAAGCGCCCGTCTTCAAGCTGGCGGCGGAAATCGCGCTGCGC
CACCATGAAAAATGGGACGGCAGCGGTTATCCGGACGGGCTGGCCGGCGAGGCGATTCCGGAATCCGCCCGCATCGTTTC
ATTGGCCGACGTGTTCGACGCGCTGACAATGCGGCGGCCATACAAGGATGCCTGGCCGATGGAGAAGGCGCTGGCCACGC
TGCGCCAGGGCAGCGGCGTCCATTTCGAGCCCAGGCTGGTGGAGGTGTTCGACGCCGCGCTGCCCAAGCTGCTGCAGATC
AAGGAAATATGGGGCGACCTGTCGCAGCAGGCCGAGCATGACGTGCTGATGCTGTTCTGA

Upstream 100 bases:

>100_bases
GCCGCTGCGCGACGCGGTGGAAAGCTTCGATTTCCGCGGCGGGGAAGCCGCCGCGCAAGCCCTGGCCGAAACGCTGGGGG
TTTCCGAGGAAAGGTGAAAC

Downstream 100 bases:

>100_bases
AACAGCCGGCTGCGGCCCGGCGCGCGGAGGCAGAGCGGATCAAACCGCCTCGACGTTGTTGTGCACCTCGCTGTCGCGCA
GCACCACCTGCGCCCACACC

Product: two-component system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQLSPPLLVVDDEPQNLAAMRQVLAPQYPLVFARNGAEALAATAKHNPSLVLLDIEMPDMDGYEVCRRIKKDPQSSSTPV
IFVTSLGEIGNEEAGFEAGAVDYLIKPVSPSIVKARVRTHLSLVQATQLEKSHRDAIYMLGQAGHFNDTDTGVHIWRMAA
YSAMLASTSGWDAAACHLLELAAPMHDTGKLGIPDAILRKPGALTPDEWEVMKTHTRIGHDILIRSEAPVFKLAAEIALR
HHEKWDGSGYPDGLAGEAIPESARIVSLADVFDALTMRRPYKDAWPMEKALATLRQGSGVHFEPRLVEVFDAALPKLLQI
KEIWGDLSQQAEHDVLMLF

Sequences:

>Translated_339_residues
MQLSPPLLVVDDEPQNLAAMRQVLAPQYPLVFARNGAEALAATAKHNPSLVLLDIEMPDMDGYEVCRRIKKDPQSSSTPV
IFVTSLGEIGNEEAGFEAGAVDYLIKPVSPSIVKARVRTHLSLVQATQLEKSHRDAIYMLGQAGHFNDTDTGVHIWRMAA
YSAMLASTSGWDAAACHLLELAAPMHDTGKLGIPDAILRKPGALTPDEWEVMKTHTRIGHDILIRSEAPVFKLAAEIALR
HHEKWDGSGYPDGLAGEAIPESARIVSLADVFDALTMRRPYKDAWPMEKALATLRQGSGVHFEPRLVEVFDAALPKLLQI
KEIWGDLSQQAEHDVLMLF
>Mature_339_residues
MQLSPPLLVVDDEPQNLAAMRQVLAPQYPLVFARNGAEALAATAKHNPSLVLLDIEMPDMDGYEVCRRIKKDPQSSSTPV
IFVTSLGEIGNEEAGFEAGAVDYLIKPVSPSIVKARVRTHLSLVQATQLEKSHRDAIYMLGQAGHFNDTDTGVHIWRMAA
YSAMLASTSGWDAAACHLLELAAPMHDTGKLGIPDAILRKPGALTPDEWEVMKTHTRIGHDILIRSEAPVFKLAAEIALR
HHEKWDGSGYPDGLAGEAIPESARIVSLADVFDALTMRRPYKDAWPMEKALATLRQGSGVHFEPRLVEVFDAALPKLLQI
KEIWGDLSQQAEHDVLMLF

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789809, Length=145, Percent_Identity=33.7931034482759, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=37.037037037037, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=135, Percent_Identity=30.3703703703704, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790860, Length=95, Percent_Identity=40, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=31.8965517241379, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=32.8125, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37247; Mature: 37247

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSPPLLVVDDEPQNLAAMRQVLAPQYPLVFARNGAEALAATAKHNPSLVLLDIEMPDM
CCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCC
DGYEVCRRIKKDPQSSSTPVIFVTSLGEIGNEEAGFEAGAVDYLIKPVSPSIVKARVRTH
CHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHH
LSLVQATQLEKSHRDAIYMLGQAGHFNDTDTGVHIWRMAAYSAMLASTSGWDAAACHLLE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAAPMHDTGKLGIPDAILRKPGALTPDEWEVMKTHTRIGHDILIRSEAPVFKLAAEIALR
HHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHH
HHEKWDGSGYPDGLAGEAIPESARIVSLADVFDALTMRRPYKDAWPMEKALATLRQGSGV
HCCCCCCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
HFEPRLVEVFDAALPKLLQIKEIWGDLSQQAEHDVLMLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MQLSPPLLVVDDEPQNLAAMRQVLAPQYPLVFARNGAEALAATAKHNPSLVLLDIEMPDM
CCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCC
DGYEVCRRIKKDPQSSSTPVIFVTSLGEIGNEEAGFEAGAVDYLIKPVSPSIVKARVRTH
CHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHH
LSLVQATQLEKSHRDAIYMLGQAGHFNDTDTGVHIWRMAAYSAMLASTSGWDAAACHLLE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAAPMHDTGKLGIPDAILRKPGALTPDEWEVMKTHTRIGHDILIRSEAPVFKLAAEIALR
HHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHH
HHEKWDGSGYPDGLAGEAIPESARIVSLADVFDALTMRRPYKDAWPMEKALATLRQGSGV
HCCCCCCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
HFEPRLVEVFDAALPKLLQIKEIWGDLSQQAEHDVLMLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]