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The map label for this gene is cheB2

Identifier: 34496464

GI number: 34496464

Start: 1047647

End: 1048717

Strand: Reverse

Name: cheB2

Synonym: CV_1009

Alternate gene names: 34496464

Gene position: 1048717-1047647 (Counterclockwise)

Preceding gene: 34496465

Following gene: 34496463

Centisome position: 22.07

GC content: 66.39

Gene sequence:

>1071_bases
ATGAGCATCAAGGTTCTGATCGTCGACGACTCCGCCGTGGTCCGGCAGGTGTTGAGCCAGGTCTTCACCGCCGCCAGCGG
CATCGAGGTGATGGATGTCGCCAACGACCCCTATATGGCGATGGACAAGATGAAGGCGCGCTGGCCGGACGTGATCGTGC
TGGACGTGGAAATGCCGCGGATGGACGGCATCACCTTCCTCAAGCAGCTGATGGCCAGCCGGCCGACGCCGGTGGTGATC
TGCTCGTCGCTGACGCAGAAAGGCACCGACATCAGCATGCAGGCGATGGCGGCCGGCGCGGTGGAGGTGATCGCCAAGCC
GGTGTCCGGCGTCAAAGGCTTTCTCGAAGAGAGCTCGCACGAACTGGTGATGGCGGTGCGCAGCGCCGCCGCCGCGCGGA
TGAACCGGGTGCGGGTGATGCCGTCCGCCAGCGGAAATCCGCTGGAGACGCGGCCCAAGCTGTCGGCCGACGCCATCCTG
TCCGCGCCGACCGGCAGCAATGTGTTCGCCACCACCGAGCGCATCATCGCCATCGGCACCTCCACCGGCGGCACCCAGGC
GCTGGAGGCGGTGCTGACCCAGCTGCCCCGCACCTGTCCCGGCCTGGCCATCGTCCAGCACATGCCGGAAAAATTCACCC
GCTCCTTCGCCGACCGGCTGAACAGCCTGTCGCAGATAGAGGTGAAGGAAGCCGACAACGGCGACCGCATCCTGCCCGGC
CGCGCGCTGATCGCCCCCGGCGGCAAGCACATGATGGTCAAGCGCAGCGGCGCCTACTACCAGGTGGAAGTCGTGGACGG
ACCGTTGGTCAGCCGCCACAAGCCATCGGTGGACGTGCTGTTCCGCTCCGCCGCCAAGTTCGCCGGCAAGAACGCGCTGG
GCATCATCATGACCGGCATGGGCGACGACGGCGCCAAGGGGCTGAAGGAAATGCACGACTGCGGCGCCAAGACCATCGCC
CAGGACGAAGAGAGCTGCGTGGTGTTCGGCATGCCCAAGGAAGCGATCAAGCTGGGCGCGGCCGACGAGGTGATGCCGCT
GGAACTGATAGCCAAGGCCATCTGCCGCTGA

Upstream 100 bases:

>100_bases
GCCCGCGCATGGTGCTGTTCGATGTCGCCAGCGGCAACGTCTGGATCAAGCAATCGCAAGAAGCCGAGCTGGAGCAGGCT
CCCCATACGAGGACGCGCAA

Downstream 100 bases:

>100_bases
ACCTCACGGACCCTGCCGAATGGACATCCACGACATCCTGGTCATCGACGACAGCCGCCTGCAGCGACAGCATGCGATGG
ATCTGTGCCGCCAGTTGGGC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSIKVLIVDDSAVVRQVLSQVFTAASGIEVMDVANDPYMAMDKMKARWPDVIVLDVEMPRMDGITFLKQLMASRPTPVVI
CSSLTQKGTDISMQAMAAGAVEVIAKPVSGVKGFLEESSHELVMAVRSAAAARMNRVRVMPSASGNPLETRPKLSADAIL
SAPTGSNVFATTERIIAIGTSTGGTQALEAVLTQLPRTCPGLAIVQHMPEKFTRSFADRLNSLSQIEVKEADNGDRILPG
RALIAPGGKHMMVKRSGAYYQVEVVDGPLVSRHKPSVDVLFRSAAKFAGKNALGIIMTGMGDDGAKGLKEMHDCGAKTIA
QDEESCVVFGMPKEAIKLGAADEVMPLELIAKAICR

Sequences:

>Translated_356_residues
MSIKVLIVDDSAVVRQVLSQVFTAASGIEVMDVANDPYMAMDKMKARWPDVIVLDVEMPRMDGITFLKQLMASRPTPVVI
CSSLTQKGTDISMQAMAAGAVEVIAKPVSGVKGFLEESSHELVMAVRSAAAARMNRVRVMPSASGNPLETRPKLSADAIL
SAPTGSNVFATTERIIAIGTSTGGTQALEAVLTQLPRTCPGLAIVQHMPEKFTRSFADRLNSLSQIEVKEADNGDRILPG
RALIAPGGKHMMVKRSGAYYQVEVVDGPLVSRHKPSVDVLFRSAAKFAGKNALGIIMTGMGDDGAKGLKEMHDCGAKTIA
QDEESCVVFGMPKEAIKLGAADEVMPLELIAKAICR
>Mature_355_residues
SIKVLIVDDSAVVRQVLSQVFTAASGIEVMDVANDPYMAMDKMKARWPDVIVLDVEMPRMDGITFLKQLMASRPTPVVIC
SSLTQKGTDISMQAMAAGAVEVIAKPVSGVKGFLEESSHELVMAVRSAAAARMNRVRVMPSASGNPLETRPKLSADAILS
APTGSNVFATTERIIAIGTSTGGTQALEAVLTQLPRTCPGLAIVQHMPEKFTRSFADRLNSLSQIEVKEADNGDRILPGR
ALIAPGGKHMMVKRSGAYYQVEVVDGPLVSRHKPSVDVLFRSAAKFAGKNALGIIMTGMGDDGAKGLKEMHDCGAKTIAQ
DEESCVVFGMPKEAIKLGAADEVMPLELIAKAICR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=350, Percent_Identity=49.4285714285714, Blast_Score=340, Evalue=7e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_CHRVO (Q7NZB3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900679.1
- ProteinModelPortal:   Q7NZB3
- SMR:   Q7NZB3
- GeneID:   2550627
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1009
- NMPDR:   fig|243365.1.peg.1009
- HOGENOM:   HBG705324
- OMA:   PGGKHML
- PhylomeDB:   Q7NZB3
- ProtClustDB:   PRK00742
- BioCyc:   CVIO243365:CV_1009-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 37924; Mature: 37793

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 181-181 ACT_SITE 207-207 ACT_SITE 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKVLIVDDSAVVRQVLSQVFTAASGIEVMDVANDPYMAMDKMKARWPDVIVLDVEMPR
CEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCC
MDGITFLKQLMASRPTPVVICSSLTQKGTDISMQAMAAGAVEVIAKPVSGVKGFLEESSH
CCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELVMAVRSAAAARMNRVRVMPSASGNPLETRPKLSADAILSAPTGSNVFATTERIIAIGT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEECCCCCCEEEECCEEEEEEC
STGGTQALEAVLTQLPRTCPGLAIVQHMPEKFTRSFADRLNSLSQIEVKEADNGDRILPG
CCCCHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHCCHHEEEEEECCCCCEECCC
RALIAPGGKHMMVKRSGAYYQVEVVDGPLVSRHKPSVDVLFRSAAKFAGKNALGIIMTGM
CEEECCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECC
GDDGAKGLKEMHDCGAKTIAQDEESCVVFGMPKEAIKLGAADEVMPLELIAKAICR
CCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SIKVLIVDDSAVVRQVLSQVFTAASGIEVMDVANDPYMAMDKMKARWPDVIVLDVEMPR
EEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCC
MDGITFLKQLMASRPTPVVICSSLTQKGTDISMQAMAAGAVEVIAKPVSGVKGFLEESSH
CCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELVMAVRSAAAARMNRVRVMPSASGNPLETRPKLSADAILSAPTGSNVFATTERIIAIGT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEECCCCCCEEEECCEEEEEEC
STGGTQALEAVLTQLPRTCPGLAIVQHMPEKFTRSFADRLNSLSQIEVKEADNGDRILPG
CCCCHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHCCHHEEEEEECCCCCEECCC
RALIAPGGKHMMVKRSGAYYQVEVVDGPLVSRHKPSVDVLFRSAAKFAGKNALGIIMTGM
CEEECCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECC
GDDGAKGLKEMHDCGAKTIAQDEESCVVFGMPKEAIKLGAADEVMPLELIAKAICR
CCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782