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The map label for this gene is phaJ [H]

Identifier: 34496361

GI number: 34496361

Start: 934944

End: 936437

Strand: Reverse

Name: phaJ [H]

Synonym: CV_0906

Alternate gene names: 34496361

Gene position: 936437-934944 (Counterclockwise)

Preceding gene: 34496362

Following gene: 34496360

Centisome position: 19.71

GC content: 72.02

Gene sequence:

>1494_bases
ATGAGCGCCTCGCAGCTGCTGTTCCTGGCGCTGACGCTGCTGACGCTGGGCCTGACCGCCGCCGCCCGCCGCCGCACCGC
CTCCCTCAATGATTTCTATACCGCCGGCGGCCGCCTCCCCCCCTGGCAGAACGGCTTGGCGCTGGCTGGCGACTACCTGT
CCGCCGCCGCCTTCCTCGGCGCGGCCGGCATGTATCTGGGCCAAGGTTACGACTCGCTGGCCTACGCGGTGGGCACGCTG
GCCGGCTGGCCGCTGCTGCTGTTGCTGCTGGCCGGACCGCTGCGCCGGCGCGGACGCTACACCGTGGCCGGCGTGCTCAG
CCAGCGTTTCGACCACCCCGGCGTGCGGCTGGTCAGCATCGCCAGTTCGCTGACGGTGACGCTGTGCTACCTGATCGTCC
AGCTGGTGGGCGCCGGCAAGTTGATCGAGCTGTTGTTCGGCCTGCCCTACCTGGCCGCCGTCGGCCTGGTCGGCGCGCTG
ATGATGGCCTACGTGGCGCTGGGCGGCATGCTGGCCGCCACCTGGGTGCAGATCAGCAAGGCCAGCCTGCTGTTCGTCTG
CGCGCTGCTGCTGGCCGCCGGCGTGCTGGCCCGCTACGGCGGCAGCCCGGCCGCGCTGTTCGCCGCCGCCGCCAAGCTGC
GCGGCGCGGCGGCCTTCCTGCCCAGCCAGGCGCTGGCCGACCCGGTGGAGGCGCTGTCGCTGGGCCTGGGCCTGAGCCTG
GGCCTCTTGGGCCTGCCGCACGTGCTGATGCGTTTCTTCACCGTGGCCGACGCCCGCGCCGCGCGCCGCTCGGTCGGCAT
CGCCACCTGGCTGGTGGCGGCCTTCTTCCTGCTCAACCTGGTCATCGGCTACGGCGCGATGACCCTGGTCGGCCCCGACC
CGCAGTTCCATGCGGTCGACGGCAAGCTGATCGGCGGCGGCAATATGGCGGCGCTGCACCTGGCCGCTCTGCTGGGCGGC
GATTTCGCGCGCGCGCTGGTGGCGGCGGTGGCCTTCGCCACCATCCTGGCGGTGGTGGCCGGCCTGATGCTGGCCGGCGC
CAGCGCGGTCAGCCACGACCTGTACGCCAGCTGGCTGAAACAGGGCCGGGCCGATCCGCGCAGCGAGCTCAAGGTTTCCC
GGCTGGCCACGCTGGGCCTGGGCCTGGCGGCGATGCTGCTGTCCACCCTGTTCCAGCAGCTGAACATCGCGGTGATGATG
GGCCTGGCCTTCGCCATCGCCGCCTCGGCCAATTTCCCGTTGCTGCTGCTCGCCTTGCACTGGCGCGGCCTGAGCGCCCG
CGGCGTGGTGTGGGGCGGCGCGGCCGGCATCGCCTCCTCGCTGGCGCTGATCGTCTGCGGCCCGGCGGTATGGATGGGCG
CGCTGGGCCACGCCGCGCCGCTGTTCCCCTATTCCAACCCGGCGCTGTTCTCGCTGCCGCTGGCCTTCGCCGCGGCCTGG
CTGGGATCGCGCCGGGCGAATCAATCAACGCATTCAAGAAAGAAAACGCAATGA

Upstream 100 bases:

>100_bases
TCGCCGGCCTGCCGCTGTCCATCCTGCTGGCGCTGGCGGTGATCCTGCTGCTGTGCTTGGTGTCCACGCTGTACAGCCGC
GTCGGCGACGGAGAGGACGC

Downstream 100 bases:

>100_bases
GCACGCTGATCCTGTACGGCAACCGCTACTCCGGCCACAGCTACAAGGTGAGGCTGGCGCTGATGCTGGCCGACATTTCC
CACGACTACGTCCACATCGA

Product: phenylacetic acid permease

Products: NA

Alternate protein names: Acetate permease; Acetate transporter ActP [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSASQLLFLALTLLTLGLTAAARRRTASLNDFYTAGGRLPPWQNGLALAGDYLSAAAFLGAAGMYLGQGYDSLAYAVGTL
AGWPLLLLLLAGPLRRRGRYTVAGVLSQRFDHPGVRLVSIASSLTVTLCYLIVQLVGAGKLIELLFGLPYLAAVGLVGAL
MMAYVALGGMLAATWVQISKASLLFVCALLLAAGVLARYGGSPAALFAAAAKLRGAAAFLPSQALADPVEALSLGLGLSL
GLLGLPHVLMRFFTVADARAARRSVGIATWLVAAFFLLNLVIGYGAMTLVGPDPQFHAVDGKLIGGGNMAALHLAALLGG
DFARALVAAVAFATILAVVAGLMLAGASAVSHDLYASWLKQGRADPRSELKVSRLATLGLGLAAMLLSTLFQQLNIAVMM
GLAFAIAASANFPLLLLALHWRGLSARGVVWGGAAGIASSLALIVCGPAVWMGALGHAAPLFPYSNPALFSLPLAFAAAW
LGSRRANQSTHSRKKTQ

Sequences:

>Translated_497_residues
MSASQLLFLALTLLTLGLTAAARRRTASLNDFYTAGGRLPPWQNGLALAGDYLSAAAFLGAAGMYLGQGYDSLAYAVGTL
AGWPLLLLLLAGPLRRRGRYTVAGVLSQRFDHPGVRLVSIASSLTVTLCYLIVQLVGAGKLIELLFGLPYLAAVGLVGAL
MMAYVALGGMLAATWVQISKASLLFVCALLLAAGVLARYGGSPAALFAAAAKLRGAAAFLPSQALADPVEALSLGLGLSL
GLLGLPHVLMRFFTVADARAARRSVGIATWLVAAFFLLNLVIGYGAMTLVGPDPQFHAVDGKLIGGGNMAALHLAALLGG
DFARALVAAVAFATILAVVAGLMLAGASAVSHDLYASWLKQGRADPRSELKVSRLATLGLGLAAMLLSTLFQQLNIAVMM
GLAFAIAASANFPLLLLALHWRGLSARGVVWGGAAGIASSLALIVCGPAVWMGALGHAAPLFPYSNPALFSLPLAFAAAW
LGSRRANQSTHSRKKTQ
>Mature_496_residues
SASQLLFLALTLLTLGLTAAARRRTASLNDFYTAGGRLPPWQNGLALAGDYLSAAAFLGAAGMYLGQGYDSLAYAVGTLA
GWPLLLLLLAGPLRRRGRYTVAGVLSQRFDHPGVRLVSIASSLTVTLCYLIVQLVGAGKLIELLFGLPYLAAVGLVGALM
MAYVALGGMLAATWVQISKASLLFVCALLLAAGVLARYGGSPAALFAAAAKLRGAAAFLPSQALADPVEALSLGLGLSLG
LLGLPHVLMRFFTVADARAARRSVGIATWLVAAFFLLNLVIGYGAMTLVGPDPQFHAVDGKLIGGGNMAALHLAALLGGD
FARALVAAVAFATILAVVAGLMLAGASAVSHDLYASWLKQGRADPRSELKVSRLATLGLGLAAMLLSTLFQQLNIAVMMG
LAFAIAASANFPLLLLALHWRGLSARGVVWGGAAGIASSLALIVCGPAVWMGALGHAAPLFPYSNPALFSLPLAFAAAWL
GSRRANQSTHSRKKTQ

Specific function: Transports acetate [H]

COG id: COG4147

COG function: function code R; Predicted symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Escherichia coli, GI1790503, Length=493, Percent_Identity=51.3184584178499, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787251, Length=363, Percent_Identity=24.5179063360882, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014083
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 51225; Mature: 51094

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCHHHHHCCCC
>Mature Secondary Structure 
SASQLLFLALTLLTLGLTAAARRRTASLNDFYTAGGRLPPWQNGLALAGDYLSAAAFLG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH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HHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA