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The map label for this gene is yafA [C]

Identifier: 34496320

GI number: 34496320

Start: 894588

End: 895829

Strand: Reverse

Name: yafA [C]

Synonym: CV_0865

Alternate gene names: 34496320

Gene position: 895829-894588 (Counterclockwise)

Preceding gene: 34496322

Following gene: 34496319

Centisome position: 18.86

GC content: 70.13

Gene sequence:

>1242_bases
ATGTCACGTTCAGAAGCGCAAAGCTGGTCGCTGGACATCGCGCTGGCACAGGGCGGTTTCGATGCCCTGCATCCGGAAGG
CAAGAACACCCTGCTGCAGCTGGGGCACGATCCGGCCGATTTCGACGCCGTGTTCGAACAGGTGAAAAGCGGCGCGATGC
TGCCCAAGGCCTGGGCCGAGGTGGCGGCCCGCGCCGAACGCCGCGCCGCCCACTACGAGCGCGACGGCTACCGCCGCACC
GCCACCGACCTCTACCTGCGCGCCGCGGTGATGTGGGGCCGCGCGCAGTACTCGATCTTCGACACCGCCGATCCGCGCAA
GGCGGTGCTGCGCGCGCGCTGCAACCACTGCGTGGCGCGCATCGGCGAGCTGCGCGACGGCCGGGTGCGGCGGGTGGAGA
TTCCGTTCGAGGGCGGCCTCATCCACGCGCTGCTGCACCTGCCTGACGGCGAAATCCGCCAGGCGCCGGCGGTGATACTC
GGCCCCGGTATGGACATGATCAAGGAAGACTACCTGTACGCCGCCGAACGCTACTACACCTCGCGCGGCATGGTGGCGCT
GTCGATAGAAGGCCCCGGCCAGGGCGAAAGCCGCGCCGGCGGCCTGGCGGTGACGCTGGACAACTACGAGCGGGCGCTGG
AAGCCTACGCCGGCTACCTGGCCGGCCTGCCGGAAGTGGACGCCGGCCGCATCGGCATGTTCGGCATCAGCATGAGCGGC
TACTGGGGCATGCGCGCCGCCGCCGGCGACACCCGGCTGGCCGCGCTGGCCGCCTACGAGCCGGTATGCGGCGACTTCCT
CACCATCTTCGAGCGCGCCCAGCCCAGCTTCAAGAAAAACTACATGTATATGAGCGGCTACCGCGACGAGGCCCTGTTCG
ACCGCGAGATGCGCGACCGCATGCCGCTGGACGCGCTAGCCGCCGACATCCGCATCCCGGTGCTGCTGGGCATCGGCGAG
TTCGACGAGCTGACGCCGCTGGAACAGGCGCTGGCCTGCTACCGGCGCATCCGCGCGCCCAAGGAGATGCGCGTATACGA
GAACGAGTTCCACCCGCTGGGCGGCGTGGCGGCCGAAGTCTTCCGCTACGGCGCGGAGTGGCTGGAGCGCGCGCTGTCCG
GCGACCCGGCATTGCGCGAGCCGGGCCGGGACGTCCGCCACTACCTGCGCCGCGACGGCCATATCGGCGACGGCAGCGCC
GCGCCGGACTGGTGGCAGGGAGCAGACCCGACGGCAGAGTAA

Upstream 100 bases:

>100_bases
GCATCCCCCTGGGGTTTGGTTGACATGCGTTCCGGCTCCACAATAATATAACTAAGTACTTAGTTATTTTTTGGTACGAT
CAATTTACGAGGTGAAAATT

Downstream 100 bases:

>100_bases
GCGCCCCGCCGGCGCCGGCAGGCGTTTGAACGATTGACGCCGCCCGGCGCCGACGCCAGACTGCCTTCATGCCAAAGACA
TGGAGGCGGTCATGAGTCAG

Product: dipeptidyl aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSRSEAQSWSLDIALAQGGFDALHPEGKNTLLQLGHDPADFDAVFEQVKSGAMLPKAWAEVAARAERRAAHYERDGYRRT
ATDLYLRAAVMWGRAQYSIFDTADPRKAVLRARCNHCVARIGELRDGRVRRVEIPFEGGLIHALLHLPDGEIRQAPAVIL
GPGMDMIKEDYLYAAERYYTSRGMVALSIEGPGQGESRAGGLAVTLDNYERALEAYAGYLAGLPEVDAGRIGMFGISMSG
YWGMRAAAGDTRLAALAAYEPVCGDFLTIFERAQPSFKKNYMYMSGYRDEALFDREMRDRMPLDALAADIRIPVLLGIGE
FDELTPLEQALACYRRIRAPKEMRVYENEFHPLGGVAAEVFRYGAEWLERALSGDPALREPGRDVRHYLRRDGHIGDGSA
APDWWQGADPTAE

Sequences:

>Translated_413_residues
MSRSEAQSWSLDIALAQGGFDALHPEGKNTLLQLGHDPADFDAVFEQVKSGAMLPKAWAEVAARAERRAAHYERDGYRRT
ATDLYLRAAVMWGRAQYSIFDTADPRKAVLRARCNHCVARIGELRDGRVRRVEIPFEGGLIHALLHLPDGEIRQAPAVIL
GPGMDMIKEDYLYAAERYYTSRGMVALSIEGPGQGESRAGGLAVTLDNYERALEAYAGYLAGLPEVDAGRIGMFGISMSG
YWGMRAAAGDTRLAALAAYEPVCGDFLTIFERAQPSFKKNYMYMSGYRDEALFDREMRDRMPLDALAADIRIPVLLGIGE
FDELTPLEQALACYRRIRAPKEMRVYENEFHPLGGVAAEVFRYGAEWLERALSGDPALREPGRDVRHYLRRDGHIGDGSA
APDWWQGADPTAE
>Mature_412_residues
SRSEAQSWSLDIALAQGGFDALHPEGKNTLLQLGHDPADFDAVFEQVKSGAMLPKAWAEVAARAERRAAHYERDGYRRTA
TDLYLRAAVMWGRAQYSIFDTADPRKAVLRARCNHCVARIGELRDGRVRRVEIPFEGGLIHALLHLPDGEIRQAPAVILG
PGMDMIKEDYLYAAERYYTSRGMVALSIEGPGQGESRAGGLAVTLDNYERALEAYAGYLAGLPEVDAGRIGMFGISMSGY
WGMRAAAGDTRLAALAAYEPVCGDFLTIFERAQPSFKKNYMYMSGYRDEALFDREMRDRMPLDALAADIRIPVLLGIGEF
DELTPLEQALACYRRIRAPKEMRVYENEFHPLGGVAAEVFRYGAEWLERALSGDPALREPGRDVRHYLRRDGHIGDGSAA
PDWWQGADPTAE

Specific function: L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6- dihydroxypseudooxyicotine [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0255 family [H]

Homologues:

Organism=Escherichia coli, GI1786434, Length=303, Percent_Identity=26.0726072607261, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010520 [H]

Pfam domain/function: PF06500 DUF1100 [H]

EC number: NA

Molecular weight: Translated: 45899; Mature: 45768

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSEAQSWSLDIALAQGGFDALHPEGKNTLLQLGHDPADFDAVFEQVKSGAMLPKAWAE
CCCCCCCCCEEEEEEECCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHCCCCCHHHHHH
VAARAERRAAHYERDGYRRTATDLYLRAAVMWGRAQYSIFDTADPRKAVLRARCNHCVAR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
IGELRDGRVRRVEIPFEGGLIHALLHLPDGEIRQAPAVILGPGMDMIKEDYLYAAERYYT
HHCCCCCCEEEEECCCCCCHHHHEEECCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHH
SRGMVALSIEGPGQGESRAGGLAVTLDNYERALEAYAGYLAGLPEVDAGRIGMFGISMSG
CCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECC
YWGMRAAAGDTRLAALAAYEPVCGDFLTIFERAQPSFKKNYMYMSGYRDEALFDREMRDR
CCCCEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCEEECCCCHHHHHHHHHHHC
MPLDALAADIRIPVLLGIGEFDELTPLEQALACYRRIRAPKEMRVYENEFHPLGGVAAEV
CCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
FRYGAEWLERALSGDPALREPGRDVRHYLRRDGHIGDGSAAPDWWQGADPTAE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRSEAQSWSLDIALAQGGFDALHPEGKNTLLQLGHDPADFDAVFEQVKSGAMLPKAWAE
CCCCCCCCEEEEEEECCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHCCCCCHHHHHH
VAARAERRAAHYERDGYRRTATDLYLRAAVMWGRAQYSIFDTADPRKAVLRARCNHCVAR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
IGELRDGRVRRVEIPFEGGLIHALLHLPDGEIRQAPAVILGPGMDMIKEDYLYAAERYYT
HHCCCCCCEEEEECCCCCCHHHHEEECCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHH
SRGMVALSIEGPGQGESRAGGLAVTLDNYERALEAYAGYLAGLPEVDAGRIGMFGISMSG
CCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECC
YWGMRAAAGDTRLAALAAYEPVCGDFLTIFERAQPSFKKNYMYMSGYRDEALFDREMRDR
CCCCEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCEEECCCCHHHHHHHHHHHC
MPLDALAADIRIPVLLGIGEFDELTPLEQALACYRRIRAPKEMRVYENEFHPLGGVAAEV
CCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
FRYGAEWLERALSGDPALREPGRDVRHYLRRDGHIGDGSAAPDWWQGADPTAE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11514508; 12618462 [H]