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The map label for this gene is arrD [H]

Identifier: 34496182

GI number: 34496182

Start: 750657

End: 751097

Strand: Reverse

Name: arrD [H]

Synonym: CV_0727

Alternate gene names: 34496182

Gene position: 751097-750657 (Counterclockwise)

Preceding gene: 34496183

Following gene: 34496181

Centisome position: 15.81

GC content: 65.53

Gene sequence:

>441_bases
ATGAAAACCAACGCAGCAGGCATCAGCCTGATCAAGCAATTCGAGGGCGTCCGCCTCGCCGCCTACCAGGACATGGTGGG
TGTCTGGACCATAGGCTACGGCCACACCGGGCCGGACGTGAAAGCCGGCATGACGATCACCCAGCAACAGGCGGACCAGT
TGCTGGCCGCCGATCTGGAGAAATTCGAAACCGGCGTCCGCAAAGCCGTGATCGTGCCGCTGAACGCCAACCAGTTCTCC
GCCTTGGTCAGCTTCAGCTACAACCTGGGGCTGGGAAACCTCCGCAGCTCCACGCTGCTGCGCCTGTTGAACAAGGGCGA
TTACGACGGCGCCGCCGCGCAGTTCCCGCGCTGGAACCGGGCCGGCGGCCAGGCCGTGCCCGGACTGACTCGCCGCCGCA
AAGCCGAGCAGGCGCTGTTCCTGACGCCTGCCCCCTGCTGA

Upstream 100 bases:

>100_bases
GCAGCAATGGCTGACGCCGCCGCCCGAGAACCCGCCCGAGACCTGAATCGCCGCCCCGGCGCCGGGGCGGCGCCCACCAC
CCTGAAATGGAGAACACGCG

Downstream 100 bases:

>100_bases
GCTTCACAAGCCGCCTCTCCCTCCGTCCTGACAACAGTCCGCCGGCTCCGCCGCGGGCTGTTTTCGCACTCAACCTCCGG
AGATACGCCATGCACAACGA

Product: phage-related lysozyme

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKTNAAGISLIKQFEGVRLAAYQDMVGVWTIGYGHTGPDVKAGMTITQQQADQLLAADLEKFETGVRKAVIVPLNANQFS
ALVSFSYNLGLGNLRSSTLLRLLNKGDYDGAAAQFPRWNRAGGQAVPGLTRRRKAEQALFLTPAPC

Sequences:

>Translated_146_residues
MKTNAAGISLIKQFEGVRLAAYQDMVGVWTIGYGHTGPDVKAGMTITQQQADQLLAADLEKFETGVRKAVIVPLNANQFS
ALVSFSYNLGLGNLRSSTLLRLLNKGDYDGAAAQFPRWNRAGGQAVPGLTRRRKAEQALFLTPAPC
>Mature_146_residues
MKTNAAGISLIKQFEGVRLAAYQDMVGVWTIGYGHTGPDVKAGMTITQQQADQLLAADLEKFETGVRKAVIVPLNANQFS
ALVSFSYNLGLGNLRSSTLLRLLNKGDYDGAAAQFPRWNRAGGQAVPGLTRRRKAEQALFLTPAPC

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=128, Percent_Identity=42.96875, Blast_Score=109, Evalue=7e-26,
Organism=Escherichia coli, GI1787836, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 15753; Mature: 15753

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNAAGISLIKQFEGVRLAAYQDMVGVWTIGYGHTGPDVKAGMTITQQQADQLLAADLE
CCCCHHHHHHHHHHCCCEEEHHHCCCEEEEECCCCCCCCCCCCCEECHHHHHHHHHHHHH
KFETGVRKAVIVPLNANQFSALVSFSYNLGLGNLRSSTLLRLLNKGDYDGAAAQFPRWNR
HHHCCCCEEEEEEECCCHHHEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
AGGQAVPGLTRRRKAEQALFLTPAPC
CCCCCCCCHHHHHHHCCEEEECCCCC
>Mature Secondary Structure
MKTNAAGISLIKQFEGVRLAAYQDMVGVWTIGYGHTGPDVKAGMTITQQQADQLLAADLE
CCCCHHHHHHHHHHCCCEEEHHHCCCEEEEECCCCCCCCCCCCCEECHHHHHHHHHHHHH
KFETGVRKAVIVPLNANQFSALVSFSYNLGLGNLRSSTLLRLLNKGDYDGAAAQFPRWNR
HHHCCCCEEEEEEECCCHHHEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
AGGQAVPGLTRRRKAEQALFLTPAPC
CCCCCCCCHHHHHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]