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The map label for this gene is rlmH

Identifier: 34495972

GI number: 34495972

Start: 550048

End: 550518

Strand: Reverse

Name: rlmH

Synonym: CV_0517

Alternate gene names: 34495972

Gene position: 550518-550048 (Counterclockwise)

Preceding gene: 34495973

Following gene: 34495967

Centisome position: 11.59

GC content: 65.82

Gene sequence:

>471_bases
ATGAAAATCACCATACTCGCCGTCGGCACCAAGATGCCGCGCTGGGTCGACGAGGCCTATACCGATTACGCCAAGCGCTT
CGGCCGCGACATCACGCTGGAACTGAAAGAGATCAAGCCGGAGAAGCGCGGCGGCGGCGTCACCGCCGAGAAAGGCATCG
CCGCCGAGCACGAGCGGCTGATCGCCGCCATCCCGCCGCGCGCGCGGCTGGTGGTGATGGACGAGCGCGGCAAGAACTGG
ACCTCGGTCAAGCTGGCCGAGGGCCTGAAGGAATGGATGGCCGGCGGCGGCGACGTGGTGTTCGTCATCGGCGGCGCCGA
CGGCCTGTCTGCGGAGCTGAAACAGCGCGCCGACGTGCTTCTGCAGCTGTCGGCGATGACCTTGCCGCACGGCATGGTGC
GGGTGATGCTGGCCGAACAGATCTACCGCGCCTATTCCATCCTCAACAACCACCCCTACCACCGCGAATAG

Upstream 100 bases:

>100_bases
CGCCGTCGGCGCGGCCAAACCCTGGTCCGCGGTGTAAGCAGGCCGGAGGGCGGGAGCTTCCCGCCCTTTTGTTTTTCCCC
CTCCGCACAAGCCCTTCCCG

Downstream 100 bases:

>100_bases
CGCGGCCGCCCGGCGGCGTCCCGCATGAAAACAGCCCCGCGGCCTGAGCCGACGGGGCTGTCGTTTTCGCTGTCTCCGCC
GCGCGCAAGGCGCGGCGGGA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKITILAVGTKMPRWVDEAYTDYAKRFGRDITLELKEIKPEKRGGGVTAEKGIAAEHERLIAAIPPRARLVVMDERGKNW
TSVKLAEGLKEWMAGGGDVVFVIGGADGLSAELKQRADVLLQLSAMTLPHGMVRVMLAEQIYRAYSILNNHPYHRE

Sequences:

>Translated_156_residues
MKITILAVGTKMPRWVDEAYTDYAKRFGRDITLELKEIKPEKRGGGVTAEKGIAAEHERLIAAIPPRARLVVMDERGKNW
TSVKLAEGLKEWMAGGGDVVFVIGGADGLSAELKQRADVLLQLSAMTLPHGMVRVMLAEQIYRAYSILNNHPYHRE
>Mature_156_residues
MKITILAVGTKMPRWVDEAYTDYAKRFGRDITLELKEIKPEKRGGGVTAEKGIAAEHERLIAAIPPRARLVVMDERGKNW
TSVKLAEGLKEWMAGGGDVVFVIGGADGLSAELKQRADVLLQLSAMTLPHGMVRVMLAEQIYRAYSILNNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=46.1538461538462, Blast_Score=142, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_CHRVO (Q7P0P9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900187.1
- ProteinModelPortal:   Q7P0P9
- SMR:   Q7P0P9
- GeneID:   2546983
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_0517
- NMPDR:   fig|243365.1.peg.517
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   Q7P0P9
- ProtClustDB:   PRK00103
- BioCyc:   CVIO243365:CV_0517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17371; Mature: 17371

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITILAVGTKMPRWVDEAYTDYAKRFGRDITLELKEIKPEKRGGGVTAEKGIAAEHERL
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHE
IAAIPPRARLVVMDERGKNWTSVKLAEGLKEWMAGGGDVVFVIGGADGLSAELKQRADVL
EEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LQLSAMTLPHGMVRVMLAEQIYRAYSILNNHPYHRE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKITILAVGTKMPRWVDEAYTDYAKRFGRDITLELKEIKPEKRGGGVTAEKGIAAEHERL
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHE
IAAIPPRARLVVMDERGKNWTSVKLAEGLKEWMAGGGDVVFVIGGADGLSAELKQRADVL
EEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LQLSAMTLPHGMVRVMLAEQIYRAYSILNNHPYHRE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782