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The map label for this gene is cobS [H]

Identifier: 34495946

GI number: 34495946

Start: 519974

End: 520717

Strand: Reverse

Name: cobS [H]

Synonym: CV_0491

Alternate gene names: 34495946

Gene position: 520717-519974 (Counterclockwise)

Preceding gene: 34495947

Following gene: 34495931

Centisome position: 10.96

GC content: 73.39

Gene sequence:

>744_bases
ATGCGCGGACTGATACTGGCGGTGCAGTTCCTGACCCGGCTGCCGACGCCGCAGCTGGCCGATTTCAAACCCGAGTGGCT
GGCGGCCAGCGCGCGCTGGTTTCCCGCCGTCGGCGTCATCGTCGGCGCGCTGCTGCTGGCGGCGCTGCAGCTGGGCAGCC
TGGCCGACCCGTGGCTGGCGGCGCTGCTGGCGCTGCTGGCCTGGGTCATGGTCACCGGCGGCCTGCACCTGGACGGCCTG
GCCGATCTGGCCGACGCGCTGGGCGCGGCCCACCGTTCGTGCGAGCGCTTCTTCGAGGTGCTGAAGGATCCGCACCTGGG
CAGCTTCGGCGTGCTGTCGCTGCTGCTGGCGGTGATCGCCAAGCTGGTGCTGCTGATGCTGATCGCACGCCAGGCCGGAA
CGGCCTGGGGCCTGCTGCTGGTCCCGGCCTGGGCGCGCGGCTTCGCCGTGATCTGGTCAGCCACGCTGCCGGCCATCGCG
CCCGGCAGCGGCGAGCGCTTCGCCTGGCGCCGCGACTGGGCCGGCATGGGGCTGAACCTGGCCGCGCTGCTGGCGCTGTC
CGCCTGGCTGGCCCCGGCGCTGCTGCTGGCCCCGCTGGCCGGCCTGGCCTGGCGGACCTTCCTGCAGCGCCGCCTGGGCG
GCATGACCGGCGACTGCCTGGGCGCCGGGGTGGAGCTGTGCGAGATCGCGATGTTGGGATTGTTGCTGTGGCGGGTGCCG
GCACCCTTCGGCTGGCTACATTAG

Upstream 100 bases:

>100_bases
AGTTCGCGGTGGCCAAGCTGATGACGGTGCAGCGCGGCGGCTTCGTCCAGCTGTCGATGTTGGAGGGCCATCCGGCCTAT
CTGCTGCGCCTGGAGTCGGC

Downstream 100 bases:

>100_bases
CCCCCCGCGAAAACGACGACGGTCCGTTTGCATGGTCACAAAACCGCACCAGGCCTCGATGATTGTGAGGCGCCCGCTTT
CCTCTTCTGGCCGCGCCGTC

Product: cobalamin (5'-phosphate) synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRGLILAVQFLTRLPTPQLADFKPEWLAASARWFPAVGVIVGALLLAALQLGSLADPWLAALLALLAWVMVTGGLHLDGL
ADLADALGAAHRSCERFFEVLKDPHLGSFGVLSLLLAVIAKLVLLMLIARQAGTAWGLLLVPAWARGFAVIWSATLPAIA
PGSGERFAWRRDWAGMGLNLAALLALSAWLAPALLLAPLAGLAWRTFLQRRLGGMTGDCLGAGVELCEIAMLGLLLWRVP
APFGWLH

Sequences:

>Translated_247_residues
MRGLILAVQFLTRLPTPQLADFKPEWLAASARWFPAVGVIVGALLLAALQLGSLADPWLAALLALLAWVMVTGGLHLDGL
ADLADALGAAHRSCERFFEVLKDPHLGSFGVLSLLLAVIAKLVLLMLIARQAGTAWGLLLVPAWARGFAVIWSATLPAIA
PGSGERFAWRRDWAGMGLNLAALLALSAWLAPALLLAPLAGLAWRTFLQRRLGGMTGDCLGAGVELCEIAMLGLLLWRVP
APFGWLH
>Mature_247_residues
MRGLILAVQFLTRLPTPQLADFKPEWLAASARWFPAVGVIVGALLLAALQLGSLADPWLAALLALLAWVMVTGGLHLDGL
ADLADALGAAHRSCERFFEVLKDPHLGSFGVLSLLLAVIAKLVLLMLIARQAGTAWGLLLVPAWARGFAVIWSATLPAIA
PGSGERFAWRRDWAGMGLNLAALLALSAWLAPALLLAPLAGLAWRTFLQRRLGGMTGDCLGAGVELCEIAMLGLLLWRVP
APFGWLH

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=239, Percent_Identity=35.9832635983264, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26315; Mature: 26315

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLILAVQFLTRLPTPQLADFKPEWLAASARWFPAVGVIVGALLLAALQLGSLADPWLA
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ALLALLAWVMVTGGLHLDGLADLADALGAAHRSCERFFEVLKDPHLGSFGVLSLLLAVIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KLVLLMLIARQAGTAWGLLLVPAWARGFAVIWSATLPAIAPGSGERFAWRRDWAGMGLNL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHH
AALLALSAWLAPALLLAPLAGLAWRTFLQRRLGGMTGDCLGAGVELCEIAMLGLLLWRVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
APFGWLH
CCCCCCC
>Mature Secondary Structure
MRGLILAVQFLTRLPTPQLADFKPEWLAASARWFPAVGVIVGALLLAALQLGSLADPWLA
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ALLALLAWVMVTGGLHLDGLADLADALGAAHRSCERFFEVLKDPHLGSFGVLSLLLAVIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KLVLLMLIARQAGTAWGLLLVPAWARGFAVIWSATLPAIAPGSGERFAWRRDWAGMGLNL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHH
AALLALSAWLAPALLLAPLAGLAWRTFLQRRLGGMTGDCLGAGVELCEIAMLGLLLWRVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
APFGWLH
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA